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The map label for this gene is rumA [H]

Identifier: 126207796

GI number: 126207796

Start: 346529

End: 347857

Strand: Reverse

Name: rumA [H]

Synonym: APL_0312

Alternate gene names: 126207796

Gene position: 347857-346529 (Counterclockwise)

Preceding gene: 126207800

Following gene: 126207793

Centisome position: 15.29

GC content: 43.12

Gene sequence:

>1329_bases
ATGGCACTGTTCTACTCTGAAAAAGCTGCAAAACAGCAAGCAAAAAAATCGGCAAAAACGACCGCTTGTCCGCCAGTTAC
CATTCAATCTTTGGATTACCAAGGTTTAGGCGTGGCGAAAATTAACGGTAAAACGTGGTTTATCGAAAATGCCTTACCTA
ACGAACAAGTTGAAATCCAAGTGTTGGAAGAAAAACGCCAATACGGACGAGCAAAAGCGGTTAAAATTTTAAAGCAATCC
GCCGCACGCCAACAGCCGAGTTGTGCTCTCTACGGCAAATGCGGCGGCAGCCAAATGCAGCATATTCCGCTGGATTTACA
GCGTGAAGCCAAACAACAGGCACTTTTTCAACGCTTACAAAAACTGCAATCACAGCCGATTGATTTTCAACCGATGATCA
TCGGTAACGATAAAGGCTATCGCCGCCGAGCAAAACTCAGTATTGCGTCACAAAATAATCAGTTAGCGATTGGTTTTAGA
ATGCAAAATTCAAACCAAATCATACCGCTTGAGCAGTGTGAGGTATTAGTCGAGCCGCTTTCTCAGCTTATCAAACCATT
GCAAAGCCTATTTAGCACATGGCAGAACAAAAAATCACTGGGACATATTGAACTGGTTCAAGCGGATGATACCATTACGA
TGTTGATACGTAACGTAGGACAATTCCATCCGCAAGACAGCCGAAACTTGCAACAATTTGCCGAACGGCATTCACTTTCA
TTGTATGTGATGACCGCTGAAAATGACATTACTCACTTAAGCGGTGAAGCGCCGTATTATCAAATTCACGGCGTTAAACT
GGGCTTTTCGATTCGTGATTTCATTCAAGTAAACGGCGACTTAAATGAAAAGATGGTAGATAAAGCGTTGGAATGGCTTG
ATCTTTCCGCACAAGATCGAGTACTGGATTTATTCTGCGGTATGGGAAACTTCACTTTGCCGATTGCCAAGCAAGCCGGC
TTTGTTGTGGGGGTGGAAGGCGTTGAGCCGATGGTTGCACAAGCTAAGCAGAATCGTGATACAAGCGGTCTAAAAAACGT
AGCATTTTACCAAACCAACCTAGACGAACCTTTTGCCGATAAACCTTGGGCAAGTGAACCGTTTAACAAAGTTTTATTAG
ATCCGGCGCGTAACGGCGCATTTTTCTGTTTAGATCATTTAGCCGCATTGAATCCCGGAACGATCGTTTATGTTTCTTGT
AATCCCGCTACCTTAGTCCGAGATGCAGAAAAACTGATTCAAGCTGGTTATAAACTGCAAAAAGCGGCAATGATCGATAT
GTTCCCACATACCGGACATTTGGAATCTATTTCGTTATTTACTAAATAA

Upstream 100 bases:

>100_bases
TTTTATCCGCCAAAAAACGTTCGGTCCAAACATTGGAAATCATCTTTCCCCCTTTTTCATATATAATAACTGACTTTACG
AATTTGATTTTACACTAAAA

Downstream 100 bases:

>100_bases
TGAACACATCATCACAGCATAAACCGAATTAGGTTGATTTATACTTAGGATATTCTGTGATGATAATCTTGAAGAAATAA
GAATTGTTTTGACAAGATAT

Product: 23S rRNA 5-methyluridine methyltransferase

Products: NA

Alternate protein names: 23S rRNA(M-5-U1939)-methyltransferase [H]

Number of amino acids: Translated: 442; Mature: 441

Protein sequence:

>442_residues
MALFYSEKAAKQQAKKSAKTTACPPVTIQSLDYQGLGVAKINGKTWFIENALPNEQVEIQVLEEKRQYGRAKAVKILKQS
AARQQPSCALYGKCGGSQMQHIPLDLQREAKQQALFQRLQKLQSQPIDFQPMIIGNDKGYRRRAKLSIASQNNQLAIGFR
MQNSNQIIPLEQCEVLVEPLSQLIKPLQSLFSTWQNKKSLGHIELVQADDTITMLIRNVGQFHPQDSRNLQQFAERHSLS
LYVMTAENDITHLSGEAPYYQIHGVKLGFSIRDFIQVNGDLNEKMVDKALEWLDLSAQDRVLDLFCGMGNFTLPIAKQAG
FVVGVEGVEPMVAQAKQNRDTSGLKNVAFYQTNLDEPFADKPWASEPFNKVLLDPARNGAFFCLDHLAALNPGTIVYVSC
NPATLVRDAEKLIQAGYKLQKAAMIDMFPHTGHLESISLFTK

Sequences:

>Translated_442_residues
MALFYSEKAAKQQAKKSAKTTACPPVTIQSLDYQGLGVAKINGKTWFIENALPNEQVEIQVLEEKRQYGRAKAVKILKQS
AARQQPSCALYGKCGGSQMQHIPLDLQREAKQQALFQRLQKLQSQPIDFQPMIIGNDKGYRRRAKLSIASQNNQLAIGFR
MQNSNQIIPLEQCEVLVEPLSQLIKPLQSLFSTWQNKKSLGHIELVQADDTITMLIRNVGQFHPQDSRNLQQFAERHSLS
LYVMTAENDITHLSGEAPYYQIHGVKLGFSIRDFIQVNGDLNEKMVDKALEWLDLSAQDRVLDLFCGMGNFTLPIAKQAG
FVVGVEGVEPMVAQAKQNRDTSGLKNVAFYQTNLDEPFADKPWASEPFNKVLLDPARNGAFFCLDHLAALNPGTIVYVSC
NPATLVRDAEKLIQAGYKLQKAAMIDMFPHTGHLESISLFTK
>Mature_441_residues
ALFYSEKAAKQQAKKSAKTTACPPVTIQSLDYQGLGVAKINGKTWFIENALPNEQVEIQVLEEKRQYGRAKAVKILKQSA
ARQQPSCALYGKCGGSQMQHIPLDLQREAKQQALFQRLQKLQSQPIDFQPMIIGNDKGYRRRAKLSIASQNNQLAIGFRM
QNSNQIIPLEQCEVLVEPLSQLIKPLQSLFSTWQNKKSLGHIELVQADDTITMLIRNVGQFHPQDSRNLQQFAERHSLSL
YVMTAENDITHLSGEAPYYQIHGVKLGFSIRDFIQVNGDLNEKMVDKALEWLDLSAQDRVLDLFCGMGNFTLPIAKQAGF
VVGVEGVEPMVAQAKQNRDTSGLKNVAFYQTNLDEPFADKPWASEPFNKVLLDPARNGAFFCLDHLAALNPGTIVYVSCN
PATLVRDAEKLIQAGYKLQKAAMIDMFPHTGHLESISLFTK

Specific function: Catalyzes the formation of 5-methyl-uridine at position 1939 (M-5-U1939) in 23S rRNA [H]

COG id: COG2265

COG function: function code J; SAM-dependent methyltransferases related to tRNA (uracil-5-)-methyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. RNA M5U methyltransferase family. RumA subfamily [H]

Homologues:

Organism=Homo sapiens, GI34222389, Length=206, Percent_Identity=29.126213592233, Blast_Score=75, Evalue=2e-13,
Organism=Homo sapiens, GI51173878, Length=206, Percent_Identity=29.126213592233, Blast_Score=75, Evalue=2e-13,
Organism=Escherichia coli, GI1789148, Length=444, Percent_Identity=46.1711711711712, Blast_Score=401, Evalue=1e-113,
Organism=Escherichia coli, GI1787083, Length=386, Percent_Identity=23.8341968911917, Blast_Score=101, Evalue=1e-22,
Organism=Escherichia coli, GI1790403, Length=373, Percent_Identity=26.5415549597855, Blast_Score=87, Evalue=2e-18,
Organism=Drosophila melanogaster, GI24666579, Length=218, Percent_Identity=30.2752293577982, Blast_Score=86, Evalue=4e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016027
- InterPro:   IPR001566
- InterPro:   IPR002792
- InterPro:   IPR010280 [H]

Pfam domain/function: PF01938 TRAM; PF05958 tRNA_U5-meth_tr [H]

EC number: =2.1.1.190 [H]

Molecular weight: Translated: 49479; Mature: 49348

Theoretical pI: Translated: 8.62; Mature: 8.62

Prosite motif: PS50926 TRAM ; PS01230 TRMA_1 ; PS01231 TRMA_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MALFYSEKAAKQQAKKSAKTTACPPVTIQSLDYQGLGVAKINGKTWFIENALPNEQVEIQ
CCEEECHHHHHHHHHHHCCCCCCCCCEEECCCCCCCEEEEECCEEEEEECCCCCCCEEEE
VLEEKRQYGRAKAVKILKQSAARQQPSCALYGKCGGSQMQHIPLDLQREAKQQALFQRLQ
EHHHHHHHCHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHH
KLQSQPIDFQPMIIGNDKGYRRRAKLSIASQNNQLAIGFRMQNSNQIIPLEQCEVLVEPL
HHHCCCCCCCCEEEECCCCCCEEEEEEEECCCCEEEEEEEECCCCCEECHHHHHHHHHHH
SQLIKPLQSLFSTWQNKKSLGHIELVQADDTITMLIRNVGQFHPQDSRNLQQFAERHSLS
HHHHHHHHHHHHHHHCCCCCCCEEEEECCCHHHHHHHHHHHCCCCCCHHHHHHHHHCCEE
LYVMTAENDITHLSGEAPYYQIHGVKLGFSIRDFIQVNGDLNEKMVDKALEWLDLSAQDR
EEEEECCCCCEECCCCCCEEEEEEEEECCCCEEHEEECCCCCHHHHHHHHHHHCCCCHHH
VLDLFCGMGNFTLPIAKQAGFVVGVEGVEPMVAQAKQNRDTSGLKNVAFYQTNLDEPFAD
HHHHHHCCCCCEEEECCCCCEEEEECCCCHHHHHHHCCCCCCCCCCEEEEECCCCCCCCC
KPWASEPFNKVLLDPARNGAFFCLDHLAALNPGTIVYVSCNPATLVRDAEKLIQAGYKLQ
CCCCCCCCHHHEECCCCCCCCHHHHHHHHCCCCEEEEEECCCHHHHHHHHHHHHCCCHHH
KAAMIDMFPHTGHLESISLFTK
HHHHEEECCCCCCCCCEEEECC
>Mature Secondary Structure 
ALFYSEKAAKQQAKKSAKTTACPPVTIQSLDYQGLGVAKINGKTWFIENALPNEQVEIQ
CEEECHHHHHHHHHHHCCCCCCCCCEEECCCCCCCEEEEECCEEEEEECCCCCCCEEEE
VLEEKRQYGRAKAVKILKQSAARQQPSCALYGKCGGSQMQHIPLDLQREAKQQALFQRLQ
EHHHHHHHCHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHH
KLQSQPIDFQPMIIGNDKGYRRRAKLSIASQNNQLAIGFRMQNSNQIIPLEQCEVLVEPL
HHHCCCCCCCCEEEECCCCCCEEEEEEEECCCCEEEEEEEECCCCCEECHHHHHHHHHHH
SQLIKPLQSLFSTWQNKKSLGHIELVQADDTITMLIRNVGQFHPQDSRNLQQFAERHSLS
HHHHHHHHHHHHHHHCCCCCCCEEEEECCCHHHHHHHHHHHCCCCCCHHHHHHHHHCCEE
LYVMTAENDITHLSGEAPYYQIHGVKLGFSIRDFIQVNGDLNEKMVDKALEWLDLSAQDR
EEEEECCCCCEECCCCCCEEEEEEEEECCCCEEHEEECCCCCHHHHHHHHHHHCCCCHHH
VLDLFCGMGNFTLPIAKQAGFVVGVEGVEPMVAQAKQNRDTSGLKNVAFYQTNLDEPFAD
HHHHHHCCCCCEEEECCCCCEEEEECCCCHHHHHHHCCCCCCCCCCEEEEECCCCCCCCC
KPWASEPFNKVLLDPARNGAFFCLDHLAALNPGTIVYVSCNPATLVRDAEKLIQAGYKLQ
CCCCCCCCHHHEECCCCCCCCHHHHHHHHCCCCEEEEEECCCHHHHHHHHHHHHCCCHHH
KAAMIDMFPHTGHLESISLFTK
HHHHEEECCCCCCCCCEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA