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The map label for this gene is nusB

Identifier: 126207689

GI number: 126207689

Start: 220866

End: 221279

Strand: Direct

Name: nusB

Synonym: APL_0201

Alternate gene names: 126207689

Gene position: 220866-221279 (Clockwise)

Preceding gene: 126207688

Following gene: 126207690

Centisome position: 9.71

GC content: 40.58

Gene sequence:

>414_bases
ATGAAAGTTTCTCCACGTCGCCGTGCCAGAGAGTGTGCGGTTCAGGCTCTTTATTCTTGGTATGTTTCGCAAAACAGTGT
AGAAGAAGTCGAATTATCATTTGTCACGGACCAAGATATGAACGGTGTTGATTTGCCTTATTTCCGTAAATTATTACGCG
GTACGGTTTTATATGTAGAAGCGATTGATAATGATCTTCGTCCGTTTTTAGACCGTGCGGAAGATGAGGTGGATCCGATT
GAACGGACCATCTTACGTCTTTCGGCATACGAATTAAAATATGAACTTGATGTACCTTATAAGGTGGTCATCAATGAAGG
TATTGAAGTGGCGAAAGTATTCGGTTCGGACGATAGCCATAAGTATATCAACGGTATTTTAGATAAATTAGCACCGGCTT
TAGGTCGTAAATAA

Upstream 100 bases:

>100_bases
TTAAATTGCAATAAGTTATTTAGTAATTGCATACGAATTTTTATGTGGCGGTTGATTAAAATACCGCCATTTTGCTCTTT
ATAATAAACTCAGAGGTTTT

Downstream 100 bases:

>100_bases
TTTATGGATAAAAAGAAGGTGAGCGTAGGTTTGCCTTCTTTTTTTGTGCTGTTACGGAGGAAAAATGATGGAATTTGAAG
TTATTGCACAATATTTTAAA

Product: transcription antitermination protein NusB

Products: NA

Alternate protein names: Protein nusB

Number of amino acids: Translated: 137; Mature: 137

Protein sequence:

>137_residues
MKVSPRRRARECAVQALYSWYVSQNSVEEVELSFVTDQDMNGVDLPYFRKLLRGTVLYVEAIDNDLRPFLDRAEDEVDPI
ERTILRLSAYELKYELDVPYKVVINEGIEVAKVFGSDDSHKYINGILDKLAPALGRK

Sequences:

>Translated_137_residues
MKVSPRRRARECAVQALYSWYVSQNSVEEVELSFVTDQDMNGVDLPYFRKLLRGTVLYVEAIDNDLRPFLDRAEDEVDPI
ERTILRLSAYELKYELDVPYKVVINEGIEVAKVFGSDDSHKYINGILDKLAPALGRK
>Mature_137_residues
MKVSPRRRARECAVQALYSWYVSQNSVEEVELSFVTDQDMNGVDLPYFRKLLRGTVLYVEAIDNDLRPFLDRAEDEVDPI
ERTILRLSAYELKYELDVPYKVVINEGIEVAKVFGSDDSHKYINGILDKLAPALGRK

Specific function: Involved in the transcription termination process

COG id: COG0781

COG function: function code K; Transcription termination factor

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the nusB family

Homologues:

Organism=Escherichia coli, GI1786618, Length=134, Percent_Identity=52.2388059701493, Blast_Score=140, Evalue=3e-35,

Paralogues:

None

Copy number: 900 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NUSB_ACTP2 (A3MYS3)

Other databases:

- EMBL:   CP000569
- RefSeq:   YP_001052914.1
- ProteinModelPortal:   A3MYS3
- SMR:   A3MYS3
- STRING:   A3MYS3
- GeneID:   4850347
- GenomeReviews:   CP000569_GR
- KEGG:   apl:APL_0201
- eggNOG:   COG0781
- HOGENOM:   HBG734409
- OMA:   IPYKVVI
- PhylomeDB:   A3MYS3
- ProtClustDB:   PRK00202
- BioCyc:   APLE416269:APL_0201-MONOMER
- HAMAP:   MF_00073
- InterPro:   IPR011605
- InterPro:   IPR006027
- Gene3D:   G3DSA:1.10.940.10
- TIGRFAMs:   TIGR01951

Pfam domain/function: PF01029 NusB; SSF48013 NusB_RsmB_TIM44

EC number: NA

Molecular weight: Translated: 15771; Mature: 15771

Theoretical pI: Translated: 4.71; Mature: 4.71

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKVSPRRRARECAVQALYSWYVSQNSVEEVELSFVTDQDMNGVDLPYFRKLLRGTVLYVE
CCCCHHHHHHHHHHHHHHHHHHCCCCHHHEEEEEECCCCCCCCCCHHHHHHHHHHHHHHH
AIDNDLRPFLDRAEDEVDPIERTILRLSAYELKYELDVPYKVVINEGIEVAKVFGSDDSH
HHCCHHHHHHHHCCCCCCHHHHHHHHHHHHHEEEECCCCEEEEECCCCCEEHCCCCCCCH
KYINGILDKLAPALGRK
HHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MKVSPRRRARECAVQALYSWYVSQNSVEEVELSFVTDQDMNGVDLPYFRKLLRGTVLYVE
CCCCHHHHHHHHHHHHHHHHHHCCCCHHHEEEEEECCCCCCCCCCHHHHHHHHHHHHHHH
AIDNDLRPFLDRAEDEVDPIERTILRLSAYELKYELDVPYKVVINEGIEVAKVFGSDDSH
HHCCHHHHHHHHCCCCCCHHHHHHHHHHHHHEEEECCCCEEEEECCCCCEEHCCCCCCCH
KYINGILDKLAPALGRK
HHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA