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The map label for this gene is dus [H]

Identifier: 126207677

GI number: 126207677

Start: 209057

End: 210019

Strand: Direct

Name: dus [H]

Synonym: APL_0189

Alternate gene names: 126207677

Gene position: 209057-210019 (Clockwise)

Preceding gene: 126207676

Following gene: 126207678

Centisome position: 9.19

GC content: 43.09

Gene sequence:

>963_bases
ATGCGTATAGGACAATATGAATTAAAAAACCGTATTTTCCTCGCGCCGATGGCAGGTATTACCGACCAACCTTTCCGCCG
TTTGTGTACTAAACTTGGAGCAAGTTTGACTTTTTCGGAGATGATGTCCACAAACCCTGAGGTATGGCATACGGAAAAAT
CTCGTTTGAGGCTTGCGCATCATGAGGAGATTGGCATCAATGCCGTACAAATTGCAGGGTCGGATCCAGCGGAAATGGCA
ATGGCGGCTAAAGTCAATGTTGAGTACGGAGCGCAAATTATTGATATTAATATGGGCTGTCCGGCTAAAAAGGTAAACAA
GAAAATGGCGGGATCCGCACTTTTACGCGAACCCGAATTAGTCGCTCAAATTTTAGATGCGGTAGTCAATGCTGTGGATG
TGCCTGTAACATTAAAAATTAGAACAGGTTGGGATCAGGAAAATAGAAACTGTTTGGAGATTGCAAAAATTGCCGAAAAG
GCAGGTATCTCCGCTTTAACGATTCACGGTCGTACGCGTCATTGCCTTTTTGAAGGTAATGCCGAATACGACAGTATTAA
AGCCGTTAAACAAGCGGTTTCTATTCCGATTATTGCTAACGGCGATATAACCTCCGCAGAGAAAGCGAAGTATGTGTTAG
ATTATACGCAAGCGGATGCGGTTATGATCGGTAGAGGCAGTTTCGGTCGCCCTTGGTTGTTTAAGGAAGTTGAACAATTT
TTAGAGAACGGTCGCCTTTTGGATTTGCCTTTGGATGAAAAATGCCGGCTCATGTTTAAGCATATCGAAGACCTTCATCG
GTTTTACGGTGAACAAAAAGGTTATCGTATCGCACGTAAACATGTCGGTTGGTATGCGGATCAATTGTCGCCAGACTTAA
CTTTTAAACGTACTTTCAATGCGTTGGATTCGACAAAAGAACAATCACTCGCATTGGAAGATTTTATGAAATCGATTCGG
TAG

Upstream 100 bases:

>100_bases
ATAGATTGATTAAAGAGTACGGTTTTTTAGCCGTACTTTTTATTTGGATAGTTATCTCGTTAAATTTGTTTTAAATTTAA
TCACTTACGGAAGAAAAAGT

Downstream 100 bases:

>100_bases
AATCCTATTTTAGTTGGATAGAGGACAATGTTAGAACAACAACCTGTACAAAACCCATTAACGGTATCAATGTTAAATGC
CCAAGCACAACAAGTGAACA

Product: tRNA-dihydrouridine synthase B

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 320; Mature: 320

Protein sequence:

>320_residues
MRIGQYELKNRIFLAPMAGITDQPFRRLCTKLGASLTFSEMMSTNPEVWHTEKSRLRLAHHEEIGINAVQIAGSDPAEMA
MAAKVNVEYGAQIIDINMGCPAKKVNKKMAGSALLREPELVAQILDAVVNAVDVPVTLKIRTGWDQENRNCLEIAKIAEK
AGISALTIHGRTRHCLFEGNAEYDSIKAVKQAVSIPIIANGDITSAEKAKYVLDYTQADAVMIGRGSFGRPWLFKEVEQF
LENGRLLDLPLDEKCRLMFKHIEDLHRFYGEQKGYRIARKHVGWYADQLSPDLTFKRTFNALDSTKEQSLALEDFMKSIR

Sequences:

>Translated_320_residues
MRIGQYELKNRIFLAPMAGITDQPFRRLCTKLGASLTFSEMMSTNPEVWHTEKSRLRLAHHEEIGINAVQIAGSDPAEMA
MAAKVNVEYGAQIIDINMGCPAKKVNKKMAGSALLREPELVAQILDAVVNAVDVPVTLKIRTGWDQENRNCLEIAKIAEK
AGISALTIHGRTRHCLFEGNAEYDSIKAVKQAVSIPIIANGDITSAEKAKYVLDYTQADAVMIGRGSFGRPWLFKEVEQF
LENGRLLDLPLDEKCRLMFKHIEDLHRFYGEQKGYRIARKHVGWYADQLSPDLTFKRTFNALDSTKEQSLALEDFMKSIR
>Mature_320_residues
MRIGQYELKNRIFLAPMAGITDQPFRRLCTKLGASLTFSEMMSTNPEVWHTEKSRLRLAHHEEIGINAVQIAGSDPAEMA
MAAKVNVEYGAQIIDINMGCPAKKVNKKMAGSALLREPELVAQILDAVVNAVDVPVTLKIRTGWDQENRNCLEIAKIAEK
AGISALTIHGRTRHCLFEGNAEYDSIKAVKQAVSIPIIANGDITSAEKAKYVLDYTQADAVMIGRGSFGRPWLFKEVEQF
LENGRLLDLPLDEKCRLMFKHIEDLHRFYGEQKGYRIARKHVGWYADQLSPDLTFKRTFNALDSTKEQSLALEDFMKSIR

Specific function: Catalyzes the synthesis of dihydrouridine, a modified base found in the D-loop of most tRNAs [H]

COG id: COG0042

COG function: function code J; tRNA-dihydrouridine synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the dus family. DusB subfamily [H]

Homologues:

Organism=Homo sapiens, GI239788483, Length=242, Percent_Identity=31.8181818181818, Blast_Score=128, Evalue=5e-30,
Organism=Homo sapiens, GI239788462, Length=237, Percent_Identity=32.0675105485232, Blast_Score=125, Evalue=4e-29,
Organism=Homo sapiens, GI31742496, Length=231, Percent_Identity=33.7662337662338, Blast_Score=114, Evalue=2e-25,
Organism=Homo sapiens, GI8923374, Length=245, Percent_Identity=31.4285714285714, Blast_Score=102, Evalue=5e-22,
Organism=Homo sapiens, GI40807366, Length=229, Percent_Identity=31.4410480349345, Blast_Score=97, Evalue=2e-20,
Organism=Escherichia coli, GI1789660, Length=319, Percent_Identity=65.8307210031348, Blast_Score=442, Evalue=1e-125,
Organism=Escherichia coli, GI1788462, Length=238, Percent_Identity=34.453781512605, Blast_Score=128, Evalue=4e-31,
Organism=Escherichia coli, GI145693211, Length=271, Percent_Identity=29.1512915129151, Blast_Score=94, Evalue=1e-20,
Organism=Caenorhabditis elegans, GI17543114, Length=263, Percent_Identity=30.7984790874525, Blast_Score=118, Evalue=4e-27,
Organism=Caenorhabditis elegans, GI25144369, Length=214, Percent_Identity=35.0467289719626, Blast_Score=102, Evalue=2e-22,
Organism=Caenorhabditis elegans, GI17510279, Length=265, Percent_Identity=34.3396226415094, Blast_Score=99, Evalue=3e-21,
Organism=Caenorhabditis elegans, GI17507177, Length=237, Percent_Identity=29.535864978903, Blast_Score=89, Evalue=4e-18,
Organism=Saccharomyces cerevisiae, GI6323433, Length=267, Percent_Identity=30.3370786516854, Blast_Score=110, Evalue=2e-25,
Organism=Saccharomyces cerevisiae, GI6323560, Length=231, Percent_Identity=29.8701298701299, Blast_Score=88, Evalue=2e-18,
Organism=Saccharomyces cerevisiae, GI6323437, Length=224, Percent_Identity=27.6785714285714, Blast_Score=87, Evalue=5e-18,
Organism=Drosophila melanogaster, GI24585320, Length=240, Percent_Identity=31.25, Blast_Score=126, Evalue=2e-29,
Organism=Drosophila melanogaster, GI24580595, Length=229, Percent_Identity=33.1877729257642, Blast_Score=112, Evalue=5e-25,
Organism=Drosophila melanogaster, GI19920448, Length=229, Percent_Identity=33.1877729257642, Blast_Score=112, Evalue=5e-25,
Organism=Drosophila melanogaster, GI19921524, Length=316, Percent_Identity=29.746835443038, Blast_Score=105, Evalue=3e-23,
Organism=Drosophila melanogaster, GI45549423, Length=262, Percent_Identity=29.0076335877863, Blast_Score=95, Evalue=6e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR004652
- InterPro:   IPR001269
- InterPro:   IPR018517 [H]

Pfam domain/function: PF01207 Dus [H]

EC number: NA

Molecular weight: Translated: 35998; Mature: 35998

Theoretical pI: Translated: 8.21; Mature: 8.21

Prosite motif: PS01136 UPF0034

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRIGQYELKNRIFLAPMAGITDQPFRRLCTKLGASLTFSEMMSTNPEVWHTEKSRLRLAH
CCCCCEECCCEEEEEECCCCCCCHHHHHHHHHCCCCCHHHHHCCCCCEEECHHHHHEEHH
HEEIGINAVQIAGSDPAEMAMAAKVNVEYGAQIIDINMGCPAKKVNKKMAGSALLREPEL
HHCCCCEEEEECCCCHHHHHHHEEEEHHHCCEEEEEECCCCHHHHHHHHHHHHHHCCHHH
VAQILDAVVNAVDVPVTLKIRTGWDQENRNCLEIAKIAEKAGISALTIHGRTRHCLFEGN
HHHHHHHHHHHHCCCEEEEEECCCCCCCCCHHHHHHHHHHCCCEEEEECCCCEEEEEECC
AEYDSIKAVKQAVSIPIIANGDITSAEKAKYVLDYTQADAVMIGRGSFGRPWLFKEVEQF
CCHHHHHHHHHHHCCCEEECCCCCCHHHHHEEEEECCCCEEEEECCCCCCCHHHHHHHHH
LENGRLLDLPLDEKCRLMFKHIEDLHRFYGEQKGYRIARKHVGWYADQLSPDLTFKRTFN
HHCCCEEECCCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCCHHHHHHH
ALDSTKEQSLALEDFMKSIR
HHHCHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MRIGQYELKNRIFLAPMAGITDQPFRRLCTKLGASLTFSEMMSTNPEVWHTEKSRLRLAH
CCCCCEECCCEEEEEECCCCCCCHHHHHHHHHCCCCCHHHHHCCCCCEEECHHHHHEEHH
HEEIGINAVQIAGSDPAEMAMAAKVNVEYGAQIIDINMGCPAKKVNKKMAGSALLREPEL
HHCCCCEEEEECCCCHHHHHHHEEEEHHHCCEEEEEECCCCHHHHHHHHHHHHHHCCHHH
VAQILDAVVNAVDVPVTLKIRTGWDQENRNCLEIAKIAEKAGISALTIHGRTRHCLFEGN
HHHHHHHHHHHHCCCEEEEEECCCCCCCCCHHHHHHHHHHCCCEEEEECCCCEEEEEECC
AEYDSIKAVKQAVSIPIIANGDITSAEKAKYVLDYTQADAVMIGRGSFGRPWLFKEVEQF
CCHHHHHHHHHHHCCCEEECCCCCCHHHHHEEEEECCCCEEEEECCCCCCCHHHHHHHHH
LENGRLLDLPLDEKCRLMFKHIEDLHRFYGEQKGYRIARKHVGWYADQLSPDLTFKRTFN
HHCCCEEECCCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCCHHHHHHH
ALDSTKEQSLALEDFMKSIR
HHHCHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA