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The map label for this gene is msbB [H]

Identifier: 126207660

GI number: 126207660

Start: 189971

End: 190927

Strand: Direct

Name: msbB [H]

Synonym: APL_0172

Alternate gene names: 126207660

Gene position: 189971-190927 (Clockwise)

Preceding gene: 126207659

Following gene: 126207662

Centisome position: 8.35

GC content: 42.11

Gene sequence:

>957_bases
ATGACAGAGCGAATTACAGCCCGAGAAGGGTATGAACATAAATTTTTAAAGGAATTTTTGTATCCGAAATATTGGGGCGT
ATGGCTTGGCGTTTTGGCGCTGGTCGTTCTGGCTTATGTGCCGGCTCGTTTACGTGATAAATTAGCCGTTTTTGTCGGAA
GAGTAGTCAAAAATTACTTAAAGAAAAAAGGCAAAAAGCAATATCATCGTGCGGATACAAACTTACGTTATTGCTTTCCG
AATTGGTCCGAACAGCAGCGTGAACAAGTGATTGAGCGAATGTTTATTACCGTAACACAAACTATGCTGGGGATTGGTGA
AATCGCAATTCGTTCGGTAAAACATTTACAACGGCGTAGTGAATTTTTAGGTTTAGCGCATCTGCAACAAGCTAAACAAG
ACGGTAAGAATATTATTTTGCTTGTGCCGCATACGTGGGCGATTGACGCTTCAGGTATTATTCTGCATACACACGGAATG
CCAATGGTTTCAATGTACAATCCGCACCGTAATGCATTAGTTGATTGGCTTTGGAATGCAACGCGCGAACGTTTCGGCGG
AAAAATGCATACACGCCAAAACGGTATTAAACCGTTTCTAAACGATGTGCGTAAAGGAAATATGGGCTATTTTTTACCTG
ATGAGGATTTCGGCGAAGAATTAAGCGTTTATGCCGATTTCTTTGCGACGGAAAAAGCCACCCTACCGGGGCTGAATAAA
ATGGCTCGTGTCGCTAATGCGGAAGTGATTCCGATGTTTCCGATTTACAATGCGGAAAAGAGCCTATATCAAATGGAAAT
TTTGCCGGCGTTGGAATTTTCCGGTGACCCGGTGCAATCCGCACGAGAAATGAATAAAGTGATTGAGTATTTTGTGACGA
AAAATCCGGAGCAATACGTATGGATTTTACGTTTACTCAAAACTCGCCGAAACGGTGAAGATATTTATAAACGCTAG

Upstream 100 bases:

>100_bases
ACTCGGGTTTATTACTGGCAATTTTACCTCCCGGTGCATTTATTGGGCTTGGGGTCATTTTGGCTGTGAAGAATTTGATT
GATAAGAAATAGAGAACCGA

Downstream 100 bases:

>100_bases
TATTGAACGATGATAAAAAACCGAGCGTTTTCGCTCGGTTTTTTTGTCGTATGAGAGGGTTAGAGTAAACCTAATACTTT
TCTGCCGTTAATTGAAGCGA

Product: lipid A biosynthesis (KDO)2-(lauroyl)-lipid IVA acyltransferase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 318; Mature: 317

Protein sequence:

>318_residues
MTERITAREGYEHKFLKEFLYPKYWGVWLGVLALVVLAYVPARLRDKLAVFVGRVVKNYLKKKGKKQYHRADTNLRYCFP
NWSEQQREQVIERMFITVTQTMLGIGEIAIRSVKHLQRRSEFLGLAHLQQAKQDGKNIILLVPHTWAIDASGIILHTHGM
PMVSMYNPHRNALVDWLWNATRERFGGKMHTRQNGIKPFLNDVRKGNMGYFLPDEDFGEELSVYADFFATEKATLPGLNK
MARVANAEVIPMFPIYNAEKSLYQMEILPALEFSGDPVQSAREMNKVIEYFVTKNPEQYVWILRLLKTRRNGEDIYKR

Sequences:

>Translated_318_residues
MTERITAREGYEHKFLKEFLYPKYWGVWLGVLALVVLAYVPARLRDKLAVFVGRVVKNYLKKKGKKQYHRADTNLRYCFP
NWSEQQREQVIERMFITVTQTMLGIGEIAIRSVKHLQRRSEFLGLAHLQQAKQDGKNIILLVPHTWAIDASGIILHTHGM
PMVSMYNPHRNALVDWLWNATRERFGGKMHTRQNGIKPFLNDVRKGNMGYFLPDEDFGEELSVYADFFATEKATLPGLNK
MARVANAEVIPMFPIYNAEKSLYQMEILPALEFSGDPVQSAREMNKVIEYFVTKNPEQYVWILRLLKTRRNGEDIYKR
>Mature_317_residues
TERITAREGYEHKFLKEFLYPKYWGVWLGVLALVVLAYVPARLRDKLAVFVGRVVKNYLKKKGKKQYHRADTNLRYCFPN
WSEQQREQVIERMFITVTQTMLGIGEIAIRSVKHLQRRSEFLGLAHLQQAKQDGKNIILLVPHTWAIDASGIILHTHGMP
MVSMYNPHRNALVDWLWNATRERFGGKMHTRQNGIKPFLNDVRKGNMGYFLPDEDFGEELSVYADFFATEKATLPGLNKM
ARVANAEVIPMFPIYNAEKSLYQMEILPALEFSGDPVQSAREMNKVIEYFVTKNPEQYVWILRLLKTRRNGEDIYKR

Specific function: Transfers myristate or laurate, activated on ACP, to (KDO)2-(lauroyl)-lipid IVA [H]

COG id: COG1560

COG function: function code M; Lauroyl/myristoyl acyltransferase

Gene ontology:

Cell location: Cell inner membrane; Single-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the htrB/msbB family [H]

Homologues:

Organism=Escherichia coli, GI1788161, Length=311, Percent_Identity=41.1575562700965, Blast_Score=271, Evalue=3e-74,
Organism=Escherichia coli, GI87082094, Length=305, Percent_Identity=29.8360655737705, Blast_Score=150, Evalue=1e-37,
Organism=Escherichia coli, GI1787292, Length=305, Percent_Identity=30.4918032786885, Blast_Score=147, Evalue=1e-36,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004960
- InterPro:   IPR011921 [H]

Pfam domain/function: PF03279 Lip_A_acyltrans [H]

EC number: 2.3.1.-

Molecular weight: Translated: 37116; Mature: 36985

Theoretical pI: Translated: 10.08; Mature: 10.08

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTERITAREGYEHKFLKEFLYPKYWGVWLGVLALVVLAYVPARLRDKLAVFVGRVVKNYL
CCCCCCCCCCCHHHHHHHHHCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHH
KKKGKKQYHRADTNLRYCFPNWSEQQREQVIERMFITVTQTMLGIGEIAIRSVKHLQRRS
HHCCHHHHHHCCCCEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
EFLGLAHLQQAKQDGKNIILLVPHTWAIDASGIILHTHGMPMVSMYNPHRNALVDWLWNA
HHHHHHHHHHHHHCCCEEEEEECCCEEECCCCEEEEECCCCEEEECCCCHHHHHHHHHHH
TRERFGGKMHTRQNGIKPFLNDVRKGNMGYFLPDEDFGEELSVYADFFATEKATLPGLNK
HHHHHCCCHHHHHCCCHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCHHH
MARVANAEVIPMFPIYNAEKSLYQMEILPALEFSGDPVQSAREMNKVIEYFVTKNPEQYV
HHHHHCCCEEEECCCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCCHHHH
WILRLLKTRRNGEDIYKR
HHHHHHHHCCCCHHHHCC
>Mature Secondary Structure 
TERITAREGYEHKFLKEFLYPKYWGVWLGVLALVVLAYVPARLRDKLAVFVGRVVKNYL
CCCCCCCCCCHHHHHHHHHCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHH
KKKGKKQYHRADTNLRYCFPNWSEQQREQVIERMFITVTQTMLGIGEIAIRSVKHLQRRS
HHCCHHHHHHCCCCEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
EFLGLAHLQQAKQDGKNIILLVPHTWAIDASGIILHTHGMPMVSMYNPHRNALVDWLWNA
HHHHHHHHHHHHHCCCEEEEEECCCEEECCCCEEEEECCCCEEEECCCCHHHHHHHHHHH
TRERFGGKMHTRQNGIKPFLNDVRKGNMGYFLPDEDFGEELSVYADFFATEKATLPGLNK
HHHHHCCCHHHHHCCCHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCHHH
MARVANAEVIPMFPIYNAEKSLYQMEILPALEFSGDPVQSAREMNKVIEYFVTKNPEQYV
HHHHHCCCEEEECCCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCCHHHH
WILRLLKTRRNGEDIYKR
HHHHHHHHCCCCHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Transferases; Acyltransferases [C]

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]