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The map label for this gene is uraA [H]

Identifier: 126207593

GI number: 126207593

Start: 120177

End: 121406

Strand: Reverse

Name: uraA [H]

Synonym: APL_0105

Alternate gene names: 126207593

Gene position: 121406-120177 (Counterclockwise)

Preceding gene: 126207594

Following gene: 126207587

Centisome position: 5.34

GC content: 46.1

Gene sequence:

>1230_bases
ATGACAAATCATCAAACTATCAGCACGCCTAAACAGGTGTTTGTTGGCTTACAAATGCTATTTGTAGCATTCGGCGCTTT
AGTATTAATGCCGTTAATTACCGGCTTAGATCCTAATACCGCCCTCCTTACCGCCGGTATCGGCACCCTACTTTTCCAAC
TTTGTACTAAAGGTCAAGTACCTATTTTCCTTGCATCTTCCTTCGCATTTATCGCACCGATTCAATACGGCGTACAGCAA
TGGGGAATCCCGACCACTATGGGTGGCTTGGTATTCACCGGCTTTGTCTATTTTGCATTAAGTGCGTTAGTAAAATGGAA
AGGGGCGAATATCCTCGAAAAACTGTTCCCGCCGGTGGTGGTTGGTCCGGTAATTATTATTATCGGTTTAGGCTTAGCCC
CGACTGCGGTGGATATGGCATTAGGAAAAGGCACAACGATTGAACCGAATACTGCCTTACTAATTTCGATGGCAACCTTA
ATCACTACACTTATCGTGGCGGTGTTTGCCAAAGGTTTAATGAAATTAGTCCCGATTATGTTTGGAATTGTGGTCGGCTA
CATTCTTTCTCTGATTTTCGGCATCATTGATTTTAGCTCAGTCACCAATGCCGCTTGGTTTAGCTTACCGAAACTCACTA
CGCCGGAATTTAAGTTAGAAGCGATTCTTTACTTACTCCCAATCGCTCTTGCACCGGCAGTCGAACACGTCGGCGGCATT
ATGGCGATCAGCTCGGTAACCGGCAAAGATTTTATGACGAAACCGGGTTTACACCGTACCTTATTAGGTGACGGTGTTGC
TACGTCTGCCGCCGCATTCTTAGGCGGTCCGCCGAATACTACCTATGCGGAAGTTACCGGTGCGGTTATGCTGACCAAAA
ACTTTAACCCGCGTGTAATGACTTTTGCCGCGATCTGGGCAATTGCAATTTCATTCTGCGGCAAAGTGGGTGCATTCTTA
CAAACCATTCCGGGCGTAGTGATGGGCGGTATTATGATGTTAGTTTTCGGTTCTATCGCAGCGGTCGGTATGAGTACATT
AATTCGTGCCAAAGTAGATATGGGCGAAGCGCGCAACCTCTGTATCGTTTCCGTGGTAATGACCTTCGGTATCGGCGGTA
TGTTGATTAACGTCGGTGAGTTCTCACTGAAAGGCATCAGCCTATGTGCTATCGCTGCAATTATTATGAACCTACTTCTT
CCAAAAGAAGCGGCTAAAAAAGCGGAATAA

Upstream 100 bases:

>100_bases
TTTCTGTTTAGCAAGTCATTTCCTTTTCAGCTATAATTCGCCGACTATCCCGTGAGGTAGTGTTTACGTATGTTAATTCA
AACCGAGAGTTGAAACAAAA

Downstream 100 bases:

>100_bases
TTGCTAACGATTCTATTCTAAAACAAGCGGTCGAATTTCACTAAAAACTTGCAAATTGCAAAAGATTTAGGAAATTCGAC
CGCTTTTTTTAAATATTCGG

Product: putative uracil permease

Products: Proton [Cytoplasm]; uracil [Cytoplasm] [C]

Alternate protein names: Uracil transporter [H]

Number of amino acids: Translated: 409; Mature: 408

Protein sequence:

>409_residues
MTNHQTISTPKQVFVGLQMLFVAFGALVLMPLITGLDPNTALLTAGIGTLLFQLCTKGQVPIFLASSFAFIAPIQYGVQQ
WGIPTTMGGLVFTGFVYFALSALVKWKGANILEKLFPPVVVGPVIIIIGLGLAPTAVDMALGKGTTIEPNTALLISMATL
ITTLIVAVFAKGLMKLVPIMFGIVVGYILSLIFGIIDFSSVTNAAWFSLPKLTTPEFKLEAILYLLPIALAPAVEHVGGI
MAISSVTGKDFMTKPGLHRTLLGDGVATSAAAFLGGPPNTTYAEVTGAVMLTKNFNPRVMTFAAIWAIAISFCGKVGAFL
QTIPGVVMGGIMMLVFGSIAAVGMSTLIRAKVDMGEARNLCIVSVVMTFGIGGMLINVGEFSLKGISLCAIAAIIMNLLL
PKEAAKKAE

Sequences:

>Translated_409_residues
MTNHQTISTPKQVFVGLQMLFVAFGALVLMPLITGLDPNTALLTAGIGTLLFQLCTKGQVPIFLASSFAFIAPIQYGVQQ
WGIPTTMGGLVFTGFVYFALSALVKWKGANILEKLFPPVVVGPVIIIIGLGLAPTAVDMALGKGTTIEPNTALLISMATL
ITTLIVAVFAKGLMKLVPIMFGIVVGYILSLIFGIIDFSSVTNAAWFSLPKLTTPEFKLEAILYLLPIALAPAVEHVGGI
MAISSVTGKDFMTKPGLHRTLLGDGVATSAAAFLGGPPNTTYAEVTGAVMLTKNFNPRVMTFAAIWAIAISFCGKVGAFL
QTIPGVVMGGIMMLVFGSIAAVGMSTLIRAKVDMGEARNLCIVSVVMTFGIGGMLINVGEFSLKGISLCAIAAIIMNLLL
PKEAAKKAE
>Mature_408_residues
TNHQTISTPKQVFVGLQMLFVAFGALVLMPLITGLDPNTALLTAGIGTLLFQLCTKGQVPIFLASSFAFIAPIQYGVQQW
GIPTTMGGLVFTGFVYFALSALVKWKGANILEKLFPPVVVGPVIIIIGLGLAPTAVDMALGKGTTIEPNTALLISMATLI
TTLIVAVFAKGLMKLVPIMFGIVVGYILSLIFGIIDFSSVTNAAWFSLPKLTTPEFKLEAILYLLPIALAPAVEHVGGIM
AISSVTGKDFMTKPGLHRTLLGDGVATSAAAFLGGPPNTTYAEVTGAVMLTKNFNPRVMTFAAIWAIAISFCGKVGAFLQ
TIPGVVMGGIMMLVFGSIAAVGMSTLIRAKVDMGEARNLCIVSVVMTFGIGGMLINVGEFSLKGISLCAIAAIIMNLLLP
KEAAKKAE

Specific function: Transport of uracil in the cell [H]

COG id: COG2233

COG function: function code F; Xanthine/uracil permeases

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the xanthine/uracil permease family. Nucleobase:cation symporter-2 (NCS2) (TC 2.A.40) subfamily [H]

Homologues:

Organism=Homo sapiens, GI44680148, Length=440, Percent_Identity=22.9545454545455, Blast_Score=74, Evalue=3e-13,
Organism=Homo sapiens, GI40316845, Length=440, Percent_Identity=22.9545454545455, Blast_Score=74, Evalue=3e-13,
Organism=Homo sapiens, GI44680145, Length=432, Percent_Identity=24.3055555555556, Blast_Score=73, Evalue=4e-13,
Organism=Homo sapiens, GI44680143, Length=435, Percent_Identity=23.9080459770115, Blast_Score=72, Evalue=1e-12,
Organism=Escherichia coli, GI1788843, Length=403, Percent_Identity=39.9503722084367, Blast_Score=280, Evalue=9e-77,
Organism=Escherichia coli, GI87081818, Length=406, Percent_Identity=35.9605911330049, Blast_Score=225, Evalue=5e-60,
Organism=Escherichia coli, GI87082181, Length=390, Percent_Identity=27.9487179487179, Blast_Score=107, Evalue=2e-24,
Organism=Escherichia coli, GI87082178, Length=242, Percent_Identity=28.5123966942149, Blast_Score=74, Evalue=2e-14,
Organism=Escherichia coli, GI1790087, Length=367, Percent_Identity=24.7956403269755, Blast_Score=62, Evalue=5e-11,
Organism=Drosophila melanogaster, GI21356175, Length=284, Percent_Identity=27.4647887323944, Blast_Score=72, Evalue=5e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006042
- InterPro:   IPR006043 [H]

Pfam domain/function: PF00860 Xan_ur_permease [H]

EC number: NA

Molecular weight: Translated: 42848; Mature: 42717

Theoretical pI: Translated: 9.61; Mature: 9.61

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
4.9 %Met     (Translated Protein)
5.9 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
4.7 %Met     (Mature Protein)
5.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTNHQTISTPKQVFVGLQMLFVAFGALVLMPLITGLDPNTALLTAGIGTLLFQLCTKGQV
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCCC
PIFLASSFAFIAPIQYGVQQWGIPTTMGGLVFTGFVYFALSALVKWKGANILEKLFPPVV
CEEEECCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHCCHHH
VGPVIIIIGLGLAPTAVDMALGKGTTIEPNTALLISMATLITTLIVAVFAKGLMKLVPIM
HHHHHHHHHCCCCHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
FGIVVGYILSLIFGIIDFSSVTNAAWFSLPKLTTPEFKLEAILYLLPIALAPAVEHVGGI
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCHH
MAISSVTGKDFMTKPGLHRTLLGDGVATSAAAFLGGPPNTTYAEVTGAVMLTKNFNPRVM
HHHHCCCCCCCCCCCCCHHHHHCCCHHHHHHHHCCCCCCCCHHHHHCEEEEECCCCCHHH
TFAAIWAIAISFCGKVGAFLQTIPGVVMGGIMMLVFGSIAAVGMSTLIRAKVDMGEARNL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCH
CIVSVVMTFGIGGMLINVGEFSLKGISLCAIAAIIMNLLLPKEAAKKAE
HHHHHHHHHCCCHHEEECCCHHCCHHHHHHHHHHHHHHHCCHHHHHCCC
>Mature Secondary Structure 
TNHQTISTPKQVFVGLQMLFVAFGALVLMPLITGLDPNTALLTAGIGTLLFQLCTKGQV
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCCC
PIFLASSFAFIAPIQYGVQQWGIPTTMGGLVFTGFVYFALSALVKWKGANILEKLFPPVV
CEEEECCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHCCHHH
VGPVIIIIGLGLAPTAVDMALGKGTTIEPNTALLISMATLITTLIVAVFAKGLMKLVPIM
HHHHHHHHHCCCCHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
FGIVVGYILSLIFGIIDFSSVTNAAWFSLPKLTTPEFKLEAILYLLPIALAPAVEHVGGI
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCHH
MAISSVTGKDFMTKPGLHRTLLGDGVATSAAAFLGGPPNTTYAEVTGAVMLTKNFNPRVM
HHHHCCCCCCCCCCCCCHHHHHCCCHHHHHHHHCCCCCCCCHHHHHCEEEEECCCCCHHH
TFAAIWAIAISFCGKVGAFLQTIPGVVMGGIMMLVFGSIAAVGMSTLIRAKVDMGEARNL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCH
CIVSVVMTFGIGGMLINVGEFSLKGISLCAIAAIIMNLLLPKEAAKKAE
HHHHHHHHHCCCHHEEECCCHHCCHHHHHHHHHHHHHHHCCHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; uracil [Periplasm] [C]

Specific reaction: Proton [Periplasm] + uracil [Periplasm] = Proton [Cytoplasm] + uracil [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11248100 [H]