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The map label for this gene is ilvG [H]

Identifier: 126207587

GI number: 126207587

Start: 104311

End: 105810

Strand: Reverse

Name: ilvG [H]

Synonym: APL_0099

Alternate gene names: 126207587

Gene position: 105810-104311 (Counterclockwise)

Preceding gene: 126207593

Following gene: 126207586

Centisome position: 4.65

GC content: 45.93

Gene sequence:

>1500_bases
ATGGCGGCTATCGGTTACGCTCGTGCGACCGGTAAAGTCGGCGTTTGTATCGCAACATCCGGCCCCGGCGCAACCAATTT
AATTACCGGCTTGGGCGATGCGGCGTTAGATTCGATTCCGGTCGTCGCAATTACCGGACAAGTGGCAAGTCATTTAATCG
GTACCGATGCGTTCCAAGAATCAGACGTATTAGGCTTATCTCTCGCTTGTACCAAACACAGCTTTATCGTGCAAAATATC
GAAGAATTACCGGAAATCATCGCACGTGCCTTCCAAATCGCGCAAAGGGGTCGCCCCGGTCCGGTATTAGTGGATATTCC
GCGCGATATTCAACTTGCACTGACCTCAGCAAAACCTATCGTTTATGAAAAACAAGCGGTTGAAGCGCAAAATCAAGACT
TATTAAACGAAGCGAAAACATTGCTGGCAAATGCCAAACGTCCGGTACTTTATGTCGGGGGCGGTGTCGGTATGGCTGGC
GGTGTGCAAGCGGTCAGAAATTTCCTAAAAGTCACAAATATTCCGTCCGTATCAACATTAAAAGGATTAGGCACAATTAC
CATTGATGATCCGCTTTATATGGGTATGATTGGTATGCACGGTACTAAAGCGGCAAACTACGCAGTACAAGAATGTGATC
TTTTAATCGCATGCGGTGCGCGTTTTGACGACCGTGTAACCGGTAAATTGGATACTTTCGCCCCACACGCTAAAGTGATT
CATATTGATATTGATATTGCCGAGATTAATAAATTACGCAAAGTGGAAGTTGCGCTACGAGGCGATTTAGTTGAAGCGGT
TAATACATTAGCGCAACCGCTAGATATTGATCCTTGGCGTGAAGACGTAAAACGCTTAAAAGCGGATTTGGATTTCCAAT
ATGTTGATAATGCGGGCGAAGGCGATATTAATGCGAAAGCGTTACTGAATACGCTTTCTCAGCGTAAACCGAAAAATGCG
ATTATTACTACCGATGTCGGTCAGCACCAAATGTGGTCGGCACAACATTTACAACATTTCGCCCCGGAAAACTTCATCAC
TTCGGCAGGTTTCGGCTCGATGGGCTTCGGTTTACCGGCTGCGGTTGGTGCGGTTAAGGCTCGTCCGCAAGATCCGGTGA
TTTTAGTTACCGGTGACGGTTCGATTATGATGAATATCCAAGAATTAGGTACGCTTAAACGCGGTCATTTACCGGTTAAG
ATCGTGTTATTGGATAACCAACGTTTAGGTATGGTGCGTCAATGGCAATCGCTGTTCTTCCACGGTCGCCACAGTAATAC
TATTTTAGACGACAATCCTGATTTCGTCGTATTAGCTTCAGCATTCGGTATTCAAGGTGAACGAATCGAAAAAGCAAGTG
AAGTATCCGATGCGTTAGATCGTTTACTCAATGCTAAAGGAGCTTATCTATTACACGTTTGTATTCCGGAAGAAGATAAC
GTATGGCCGCTTGTGCCGCCGGGTGCGTGTAATGCGGATATGCTGGATGATGAGATTTAG

Upstream 100 bases:

>100_bases
CCTTTTCGGTTATCCGGGCGGTGCAATCATGCCTACCTACGATGCGATTTACGACTCGGGATTAGATCACTTATTAGTAC
GCAACGAACAAGGTGCGGCG

Downstream 100 bases:

>100_bases
TGATTGAGGAGAATGAAGAAATGCAACGTTATGATTTAACGCTTAAAGCGGAAAAACGTCCTGAAACGCTGGAGCGTATT
TTACGTGTTGTACGCCATCG

Product: acetolactate synthase isozyme II large subunit (AHAS-II)

Products: NA

Alternate protein names: AHAS-II; ALS-II; Acetohydroxy-acid synthase II large subunit [H]

Number of amino acids: Translated: 499; Mature: 498

Protein sequence:

>499_residues
MAAIGYARATGKVGVCIATSGPGATNLITGLGDAALDSIPVVAITGQVASHLIGTDAFQESDVLGLSLACTKHSFIVQNI
EELPEIIARAFQIAQRGRPGPVLVDIPRDIQLALTSAKPIVYEKQAVEAQNQDLLNEAKTLLANAKRPVLYVGGGVGMAG
GVQAVRNFLKVTNIPSVSTLKGLGTITIDDPLYMGMIGMHGTKAANYAVQECDLLIACGARFDDRVTGKLDTFAPHAKVI
HIDIDIAEINKLRKVEVALRGDLVEAVNTLAQPLDIDPWREDVKRLKADLDFQYVDNAGEGDINAKALLNTLSQRKPKNA
IITTDVGQHQMWSAQHLQHFAPENFITSAGFGSMGFGLPAAVGAVKARPQDPVILVTGDGSIMMNIQELGTLKRGHLPVK
IVLLDNQRLGMVRQWQSLFFHGRHSNTILDDNPDFVVLASAFGIQGERIEKASEVSDALDRLLNAKGAYLLHVCIPEEDN
VWPLVPPGACNADMLDDEI

Sequences:

>Translated_499_residues
MAAIGYARATGKVGVCIATSGPGATNLITGLGDAALDSIPVVAITGQVASHLIGTDAFQESDVLGLSLACTKHSFIVQNI
EELPEIIARAFQIAQRGRPGPVLVDIPRDIQLALTSAKPIVYEKQAVEAQNQDLLNEAKTLLANAKRPVLYVGGGVGMAG
GVQAVRNFLKVTNIPSVSTLKGLGTITIDDPLYMGMIGMHGTKAANYAVQECDLLIACGARFDDRVTGKLDTFAPHAKVI
HIDIDIAEINKLRKVEVALRGDLVEAVNTLAQPLDIDPWREDVKRLKADLDFQYVDNAGEGDINAKALLNTLSQRKPKNA
IITTDVGQHQMWSAQHLQHFAPENFITSAGFGSMGFGLPAAVGAVKARPQDPVILVTGDGSIMMNIQELGTLKRGHLPVK
IVLLDNQRLGMVRQWQSLFFHGRHSNTILDDNPDFVVLASAFGIQGERIEKASEVSDALDRLLNAKGAYLLHVCIPEEDN
VWPLVPPGACNADMLDDEI
>Mature_498_residues
AAIGYARATGKVGVCIATSGPGATNLITGLGDAALDSIPVVAITGQVASHLIGTDAFQESDVLGLSLACTKHSFIVQNIE
ELPEIIARAFQIAQRGRPGPVLVDIPRDIQLALTSAKPIVYEKQAVEAQNQDLLNEAKTLLANAKRPVLYVGGGVGMAGG
VQAVRNFLKVTNIPSVSTLKGLGTITIDDPLYMGMIGMHGTKAANYAVQECDLLIACGARFDDRVTGKLDTFAPHAKVIH
IDIDIAEINKLRKVEVALRGDLVEAVNTLAQPLDIDPWREDVKRLKADLDFQYVDNAGEGDINAKALLNTLSQRKPKNAI
ITTDVGQHQMWSAQHLQHFAPENFITSAGFGSMGFGLPAAVGAVKARPQDPVILVTGDGSIMMNIQELGTLKRGHLPVKI
VLLDNQRLGMVRQWQSLFFHGRHSNTILDDNPDFVVLASAFGIQGERIEKASEVSDALDRLLNAKGAYLLHVCIPEEDNV
WPLVPPGACNADMLDDEI

Specific function: Catalyzes the first step in the biosynthesis of branched-chain amino acids [H]

COG id: COG0028

COG function: function code EH; Thiamine pyrophosphate-requiring enzymes [acetolactate synthase, pyruvate dehydrogenase (cytochrome), glyoxylate carboligase, phosphonopyruvate decarboxylase]

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TPP enzyme family [H]

Homologues:

Organism=Homo sapiens, GI93004078, Length=488, Percent_Identity=23.9754098360656, Blast_Score=138, Evalue=1e-32,
Organism=Homo sapiens, GI21361361, Length=448, Percent_Identity=23.2142857142857, Blast_Score=100, Evalue=5e-21,
Organism=Escherichia coli, GI1790104, Length=500, Percent_Identity=44.4, Blast_Score=418, Evalue=1e-118,
Organism=Escherichia coli, GI87081685, Length=503, Percent_Identity=41.948310139165, Blast_Score=380, Evalue=1e-106,
Organism=Escherichia coli, GI1786717, Length=483, Percent_Identity=29.6066252587992, Blast_Score=219, Evalue=3e-58,
Organism=Escherichia coli, GI1787096, Length=484, Percent_Identity=29.3388429752066, Blast_Score=186, Evalue=3e-48,
Organism=Escherichia coli, GI1788716, Length=489, Percent_Identity=24.3353783231084, Blast_Score=115, Evalue=6e-27,
Organism=Caenorhabditis elegans, GI17531299, Length=431, Percent_Identity=29.4663573085847, Blast_Score=127, Evalue=1e-29,
Organism=Caenorhabditis elegans, GI17531301, Length=431, Percent_Identity=29.4663573085847, Blast_Score=127, Evalue=2e-29,
Organism=Caenorhabditis elegans, GI17542570, Length=520, Percent_Identity=24.6153846153846, Blast_Score=111, Evalue=9e-25,
Organism=Saccharomyces cerevisiae, GI6323755, Length=515, Percent_Identity=41.1650485436893, Blast_Score=384, Evalue=1e-107,
Organism=Drosophila melanogaster, GI19922626, Length=431, Percent_Identity=27.6102088167053, Blast_Score=137, Evalue=2e-32,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012846
- InterPro:   IPR012000
- InterPro:   IPR012001
- InterPro:   IPR000399
- InterPro:   IPR011766 [H]

Pfam domain/function: PF02775 TPP_enzyme_C; PF00205 TPP_enzyme_M; PF02776 TPP_enzyme_N [H]

EC number: =2.2.1.6 [H]

Molecular weight: Translated: 53569; Mature: 53438

Theoretical pI: Translated: 5.44; Mature: 5.44

Prosite motif: PS00187 TPP_ENZYMES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAAIGYARATGKVGVCIATSGPGATNLITGLGDAALDSIPVVAITGQVASHLIGTDAFQE
CCCCCCEECCCCEEEEEEECCCCHHHHHHHCCHHHHCCCCEEEEEHHHHHHHHCCCCCCC
SDVLGLSLACTKHSFIVQNIEELPEIIARAFQIAQRGRPGPVLVDIPRDIQLALTSAKPI
CCCEEEEEEECHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEECCCCEEEEEECCCCE
VYEKQAVEAQNQDLLNEAKTLLANAKRPVLYVGGGVGMAGGVQAVRNFLKVTNIPSVSTL
EEEHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCHHHHHHHHHHHHCCCCCHHHH
KGLGTITIDDPLYMGMIGMHGTKAANYAVQECDLLIACGARFDDRVTGKLDTFAPHAKVI
CCCEEEEECCCHHHHEECCCCCCHHHHHHHHCCEEEEECCCCCCCCCCCCCCCCCCCEEE
HIDIDIAEINKLRKVEVALRGDLVEAVNTLAQPLDIDPWREDVKRLKADLDFQYVDNAGE
EEEEEHHHHHHHHEEHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCEEEECCCCC
GDINAKALLNTLSQRKPKNAIITTDVGQHQMWSAQHLQHFAPENFITSAGFGSMGFGLPA
CCCHHHHHHHHHHHCCCCCCEEEECCCCHHHHHHHHHHHHCCCCCEECCCCCCCCCCCHH
AVGAVKARPQDPVILVTGDGSIMMNIQELGTLKRGHLPVKIVLLDNQRLGMVRQWQSLFF
HHHHHCCCCCCCEEEEECCCCEEEEHHHHCCCCCCCCCEEEEEECCCCCCHHHHHHHHHE
HGRHSNTILDDNPDFVVLASAFGIQGERIEKASEVSDALDRLLNAKGAYLLHVCIPEEDN
ECCCCCCEECCCCCEEEEEEECCCCCHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCC
VWPLVPPGACNADMLDDEI
EEECCCCCCCCCCCCCCCC
>Mature Secondary Structure 
AAIGYARATGKVGVCIATSGPGATNLITGLGDAALDSIPVVAITGQVASHLIGTDAFQE
CCCCCEECCCCEEEEEEECCCCHHHHHHHCCHHHHCCCCEEEEEHHHHHHHHCCCCCCC
SDVLGLSLACTKHSFIVQNIEELPEIIARAFQIAQRGRPGPVLVDIPRDIQLALTSAKPI
CCCEEEEEEECHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEECCCCEEEEEECCCCE
VYEKQAVEAQNQDLLNEAKTLLANAKRPVLYVGGGVGMAGGVQAVRNFLKVTNIPSVSTL
EEEHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCHHHHHHHHHHHHCCCCCHHHH
KGLGTITIDDPLYMGMIGMHGTKAANYAVQECDLLIACGARFDDRVTGKLDTFAPHAKVI
CCCEEEEECCCHHHHEECCCCCCHHHHHHHHCCEEEEECCCCCCCCCCCCCCCCCCCEEE
HIDIDIAEINKLRKVEVALRGDLVEAVNTLAQPLDIDPWREDVKRLKADLDFQYVDNAGE
EEEEEHHHHHHHHEEHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCEEEECCCCC
GDINAKALLNTLSQRKPKNAIITTDVGQHQMWSAQHLQHFAPENFITSAGFGSMGFGLPA
CCCHHHHHHHHHHHCCCCCCEEEECCCCHHHHHHHHHHHHCCCCCEECCCCCCCCCCCHH
AVGAVKARPQDPVILVTGDGSIMMNIQELGTLKRGHLPVKIVLLDNQRLGMVRQWQSLFF
HHHHHCCCCCCCEEEEECCCCEEEEHHHHCCCCCCCCCEEEEEECCCCCCHHHHHHHHHE
HGRHSNTILDDNPDFVVLASAFGIQGERIEKASEVSDALDRLLNAKGAYLLHVCIPEEDN
ECCCCCCEECCCCCEEEEEEECCCCCHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCC
VWPLVPPGACNADMLDDEI
EEECCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 3550695; 1379743; 9278503; 1995430; 6154938; 7015336; 3897211 [H]