Click here to switch to the map view.

The map label for this gene is nrfC [H]

Identifier: 126207590

GI number: 126207590

Start: 108539

End: 109216

Strand: Direct

Name: nrfC [H]

Synonym: APL_0102

Alternate gene names: 126207590

Gene position: 108539-109216 (Clockwise)

Preceding gene: 126207589

Following gene: 126207591

Centisome position: 4.77

GC content: 46.17

Gene sequence:

>678_bases
ATGACTTGCACCCGTAGAGACTTCGTCTCAGGGGCAGGTGCTATCGCTGTCGTAGCAAGCACTGGGCTTGCTACCTCAGT
AACTCTTGCTACTGAGAAAGAAGAGAAAAAAATTCGCTATGCGATGGTGCACGATGAAACCTCTTGTATCGGTTGTACCG
CTTGTATGGACGCATGTCGTACGACAAACAAAGTACCGGAAGGCGTTTCACGTTTAGAAATTATCCGTAGCGAGCCTTAT
GGCGAATTCCCTAATGTCGAGTATGAATTTTTCCGTCAATCTTGCCAACACTGTACGAATGCTCCGTGCGTAGCGGTATG
TCCGACTGGCGCTTCATTCGTAGATCCGGAAACCGGCATTGTTGATGTACATTCAGACTTATGTGTCGGTTGCCAATACT
GTATCGCAGTTTGCCCGTACCGTGTACGTTTTATCCATCCGGAGAAAAAATCCGCAGACAAATGTAACTTCTGTCGTGAT
ACTAACCTTGCGGAAGGTAAACAACCGGCTTGTGTAGAAGCATGCCCGACTAAAGCATTAACCTTTGGCGATATGAACGA
TCACAATAGCGAAGTGTACAAAAAAGTGCGTGAAAATCCTGTGTATCGTACTAAAGTTGCGTTAGGTACCGAGCCTAACT
TATACCACATTCCATTTGGCAAGGGGGAGCATAGATAA

Upstream 100 bases:

>100_bases
AAGAAAAACAACGTATTAAACTTTGCGTTGATTGTCATACCAAAGTTCAAAAAGGCGAGTTTAACACTCCGGCAAAAACA
GAAGTGAAGGAAACCAAATA

Downstream 100 bases:

>100_bases
TGAACAGCTATATTCCGTTCCAAACCCCGAATTTAGTGTGGGACAGCACCATTGCGATTTACCTTTTCTTATTGGGTATT
TCATCAGGTGCGGTGCAGTT

Product: nitrate reductase

Products: oxidized cytochrome C552; NH3 [C]

Alternate protein names: NA

Number of amino acids: Translated: 225; Mature: 224

Protein sequence:

>225_residues
MTCTRRDFVSGAGAIAVVASTGLATSVTLATEKEEKKIRYAMVHDETSCIGCTACMDACRTTNKVPEGVSRLEIIRSEPY
GEFPNVEYEFFRQSCQHCTNAPCVAVCPTGASFVDPETGIVDVHSDLCVGCQYCIAVCPYRVRFIHPEKKSADKCNFCRD
TNLAEGKQPACVEACPTKALTFGDMNDHNSEVYKKVRENPVYRTKVALGTEPNLYHIPFGKGEHR

Sequences:

>Translated_225_residues
MTCTRRDFVSGAGAIAVVASTGLATSVTLATEKEEKKIRYAMVHDETSCIGCTACMDACRTTNKVPEGVSRLEIIRSEPY
GEFPNVEYEFFRQSCQHCTNAPCVAVCPTGASFVDPETGIVDVHSDLCVGCQYCIAVCPYRVRFIHPEKKSADKCNFCRD
TNLAEGKQPACVEACPTKALTFGDMNDHNSEVYKKVRENPVYRTKVALGTEPNLYHIPFGKGEHR
>Mature_224_residues
TCTRRDFVSGAGAIAVVASTGLATSVTLATEKEEKKIRYAMVHDETSCIGCTACMDACRTTNKVPEGVSRLEIIRSEPYG
EFPNVEYEFFRQSCQHCTNAPCVAVCPTGASFVDPETGIVDVHSDLCVGCQYCIAVCPYRVRFIHPEKKSADKCNFCRDT
NLAEGKQPACVEACPTKALTFGDMNDHNSEVYKKVRENPVYRTKVALGTEPNLYHIPFGKGEHR

Specific function: Probably involved in the transfer of electrons from the quinone pool to the type-c cytochromes [H]

COG id: COG0437

COG function: function code C; Fe-S-cluster-containing hydrogenase components 1

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 3 4Fe-4S ferredoxin-type domains [H]

Homologues:

Organism=Escherichia coli, GI2367345, Length=224, Percent_Identity=66.5178571428571, Blast_Score=313, Evalue=7e-87,
Organism=Escherichia coli, GI226510944, Length=215, Percent_Identity=47.906976744186, Blast_Score=200, Evalue=8e-53,
Organism=Escherichia coli, GI1789370, Length=223, Percent_Identity=31.8385650224215, Blast_Score=105, Evalue=3e-24,
Organism=Escherichia coli, GI1790326, Length=189, Percent_Identity=29.6296296296296, Blast_Score=102, Evalue=3e-23,
Organism=Escherichia coli, GI1787749, Length=174, Percent_Identity=30.4597701149425, Blast_Score=100, Evalue=8e-23,
Organism=Escherichia coli, GI1787872, Length=192, Percent_Identity=34.375, Blast_Score=94, Evalue=1e-20,
Organism=Escherichia coli, GI1787122, Length=192, Percent_Identity=33.8541666666667, Blast_Score=92, Evalue=2e-20,
Organism=Escherichia coli, GI1787740, Length=145, Percent_Identity=30.3448275862069, Blast_Score=78, Evalue=4e-16,
Organism=Escherichia coli, GI1787478, Length=142, Percent_Identity=28.8732394366197, Blast_Score=72, Evalue=4e-14,
Organism=Escherichia coli, GI2367245, Length=173, Percent_Identity=31.2138728323699, Blast_Score=71, Evalue=7e-14,
Organism=Escherichia coli, GI87082179, Length=149, Percent_Identity=28.8590604026846, Blast_Score=67, Evalue=8e-13,
Organism=Escherichia coli, GI1789067, Length=176, Percent_Identity=27.2727272727273, Blast_Score=65, Evalue=2e-12,
Organism=Escherichia coli, GI87082180, Length=169, Percent_Identity=30.7692307692308, Blast_Score=65, Evalue=4e-12,
Organism=Escherichia coli, GI1789079, Length=191, Percent_Identity=28.7958115183246, Blast_Score=64, Evalue=7e-12,
Organism=Escherichia coli, GI1788811, Length=152, Percent_Identity=30.9210526315789, Blast_Score=62, Evalue=3e-11,
Organism=Escherichia coli, GI1787963, Length=202, Percent_Identity=24.7524752475248, Blast_Score=62, Evalue=3e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017896
- InterPro:   IPR017900
- InterPro:   IPR017567
- InterPro:   IPR006311 [H]

Pfam domain/function: NA

EC number: NA

Molecular weight: Translated: 24792; Mature: 24661

Theoretical pI: Translated: 6.81; Mature: 6.81

Prosite motif: PS00198 4FE4S_FERREDOXIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

7.6 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
9.3 %Cys+Met (Translated Protein)
7.6 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
8.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTCTRRDFVSGAGAIAVVASTGLATSVTLATEKEEKKIRYAMVHDETSCIGCTACMDACR
CCCCCCHHHCCCCEEEEEEECCCEEEEEEECCCHHHHEEEEEEECCCCHHHHHHHHHHHH
TTNKVPEGVSRLEIIRSEPYGEFPNVEYEFFRQSCQHCTNAPCVAVCPTGASFVDPETGI
HCCCCCCHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHCCCCEEEECCCCCCCCCCCCCE
VDVHSDLCVGCQYCIAVCPYRVRFIHPEKKSADKCNFCRDTNLAEGKQPACVEACPTKAL
EECCHHHHHHHHHHHHHCCEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCHHHHCCCCEE
TFGDMNDHNSEVYKKVRENPVYRTKVALGTEPNLYHIPFGKGEHR
EECCCCCCHHHHHHHHHCCCCEEEEEEECCCCCEEECCCCCCCCC
>Mature Secondary Structure 
TCTRRDFVSGAGAIAVVASTGLATSVTLATEKEEKKIRYAMVHDETSCIGCTACMDACR
CCCCCHHHCCCCEEEEEEECCCEEEEEEECCCHHHHEEEEEEECCCCHHHHHHHHHHHH
TTNKVPEGVSRLEIIRSEPYGEFPNVEYEFFRQSCQHCTNAPCVAVCPTGASFVDPETGI
HCCCCCCHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHCCCCEEEECCCCCCCCCCCCCE
VDVHSDLCVGCQYCIAVCPYRVRFIHPEKKSADKCNFCRDTNLAEGKQPACVEACPTKAL
EECCHHHHHHHHHHHHHCCEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCHHHHCCCCEE
TFGDMNDHNSEVYKKVRENPVYRTKVALGTEPNLYHIPFGKGEHR
EECCCCCCHHHHHHHHHCCCCEEEEEEECCCCCEEECCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: Fe [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: reduced cytochrome C552; nitrite [C]

Specific reaction: reduced cytochrome C552 + nitrite = oxidized cytochrome C552 + NH3 [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]