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The map label for this gene is nrfD [H]

Identifier: 126207591

GI number: 126207591

Start: 109216

End: 110184

Strand: Direct

Name: nrfD [H]

Synonym: APL_0103

Alternate gene names: 126207591

Gene position: 109216-110184 (Clockwise)

Preceding gene: 126207590

Following gene: 126207592

Centisome position: 4.8

GC content: 41.49

Gene sequence:

>969_bases
ATGAACAGCTATATTCCGTTCCAAACCCCGAATTTAGTGTGGGACAGCACCATTGCGATTTACCTTTTCTTATTGGGTAT
TTCATCAGGTGCGGTGCAGTTGGCGATTGCTTATCGTAATAGCGGTGCCAAAATTGCAAAAAACAGCGATAACTGGGTTA
TCCGTAGTGCGGCGATTTTAGGTACGTTACCGACGCTTATCGGTTTAACATTATTAATTTTCCACTTGACCAAACCGTGG
ACATTCTGGAAATTAATGTTTAACTATAACGGTACTTCCGTAATGTCGATGGGGGTAATGTTATTCCAAGTTTACATGGC
TGTGCTTGTAGTTTGGATTGCCATTATGTTCAAAGATTGGCTGGCTGTTTTAGTGAATCGCTACTTACCGATGTTTAAAT
TCGTATTACCGTTGGTTGATTTTGCCGAAACTCGCTTGCTTAAAGCGATCGAATTCGTACTCTTTGTATTAGCGGCAGTG
TTAGGGGCTTATCCCGGTTTCCTACTTTCTGCATTAATCAGTTATCCGATGTTGAATAACCCTGTATTACCGGCGTTATT
CTTAGCATCAGGTACGTCCTCAGGTATTGCGGCAACATTCTTAGTGATTCTGCTTGCCGGTAAATTATCCGGCGAAAGTA
ACGAAGTACATTTTATGCATAAATTCGAAGTACCGATTATGGTTACCGAATTAGGTTTACTCGTTGCATTCTTCGTCGGT
TTACACTTCGGCGGCGCAGATAAACAGTTGGCATTACAAAACGCCTTATCAGGCTTCTGGGGAACGATATTCTGGGTTGG
GGTGTTCTTTATCGGTATCTTAATCCCGTTGGTAGCGAATATCTTCGGCAGCCATAGCTTGAAACATAATGTTAAATTTA
TCATTTTAGTTTCAATCTTCGACCTAATCGGCGTACTCTGCTTACGTTACTTTATCCTCTATGCAGGACAATTAACCGTA
GCGAGTTAA

Upstream 100 bases:

>100_bases
ACAAAAAAGTGCGTGAAAATCCTGTGTATCGTACTAAAGTTGCGTTAGGTACCGAGCCTAACTTATACCACATTCCATTT
GGCAAGGGGGAGCATAGATA

Downstream 100 bases:

>100_bases
TTTAATCTATTTCTAATAAATAAAAACTCTGTACTTGAAATCGGATTTCACTTTGGTTTTGAGGCAGAGTTTTTTCGTTT
TTATTCATAGTAAGAGCGGT

Product: nitrite reductase transmembrane protein

Products: oxidized cytochrome C552; NH3 [C]

Alternate protein names: NA

Number of amino acids: Translated: 322; Mature: 322

Protein sequence:

>322_residues
MNSYIPFQTPNLVWDSTIAIYLFLLGISSGAVQLAIAYRNSGAKIAKNSDNWVIRSAAILGTLPTLIGLTLLIFHLTKPW
TFWKLMFNYNGTSVMSMGVMLFQVYMAVLVVWIAIMFKDWLAVLVNRYLPMFKFVLPLVDFAETRLLKAIEFVLFVLAAV
LGAYPGFLLSALISYPMLNNPVLPALFLASGTSSGIAATFLVILLAGKLSGESNEVHFMHKFEVPIMVTELGLLVAFFVG
LHFGGADKQLALQNALSGFWGTIFWVGVFFIGILIPLVANIFGSHSLKHNVKFIILVSIFDLIGVLCLRYFILYAGQLTV
AS

Sequences:

>Translated_322_residues
MNSYIPFQTPNLVWDSTIAIYLFLLGISSGAVQLAIAYRNSGAKIAKNSDNWVIRSAAILGTLPTLIGLTLLIFHLTKPW
TFWKLMFNYNGTSVMSMGVMLFQVYMAVLVVWIAIMFKDWLAVLVNRYLPMFKFVLPLVDFAETRLLKAIEFVLFVLAAV
LGAYPGFLLSALISYPMLNNPVLPALFLASGTSSGIAATFLVILLAGKLSGESNEVHFMHKFEVPIMVTELGLLVAFFVG
LHFGGADKQLALQNALSGFWGTIFWVGVFFIGILIPLVANIFGSHSLKHNVKFIILVSIFDLIGVLCLRYFILYAGQLTV
AS
>Mature_322_residues
MNSYIPFQTPNLVWDSTIAIYLFLLGISSGAVQLAIAYRNSGAKIAKNSDNWVIRSAAILGTLPTLIGLTLLIFHLTKPW
TFWKLMFNYNGTSVMSMGVMLFQVYMAVLVVWIAIMFKDWLAVLVNRYLPMFKFVLPLVDFAETRLLKAIEFVLFVLAAV
LGAYPGFLLSALISYPMLNNPVLPALFLASGTSSGIAATFLVILLAGKLSGESNEVHFMHKFEVPIMVTELGLLVAFFVG
LHFGGADKQLALQNALSGFWGTIFWVGVFFIGILIPLVANIFGSHSLKHNVKFIILVSIFDLIGVLCLRYFILYAGQLTV
AS

Specific function: Probably involved in the transfer of electrons from the quinone pool to the type-c cytochromes [H]

COG id: COG3301

COG function: function code P; Formate-dependent nitrite reductase, membrane component

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the nrfD family [H]

Homologues:

Organism=Escherichia coli, GI1790509, Length=322, Percent_Identity=47.2049689440994, Blast_Score=260, Evalue=8e-71,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017566
- InterPro:   IPR005614 [H]

Pfam domain/function: PF03916 NrfD [H]

EC number: NA

Molecular weight: Translated: 35534; Mature: 35534

Theoretical pI: Translated: 9.61; Mature: 9.61

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNSYIPFQTPNLVWDSTIAIYLFLLGISSGAVQLAIAYRNSGAKIAKNSDNWVIRSAAIL
CCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCEEEEEEEECCCCEEEECCCCEEEEHHHHH
GTLPTLIGLTLLIFHLTKPWTFWKLMFNYNGTSVMSMGVMLFQVYMAVLVVWIAIMFKDW
HHHHHHHHHHHHHHHHCCCCEEEHEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LAVLVNRYLPMFKFVLPLVDFAETRLLKAIEFVLFVLAAVLGAYPGFLLSALISYPMLNN
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHCCCCCC
PVLPALFLASGTSSGIAATFLVILLAGKLSGESNEVHFMHKFEVPIMVTELGLLVAFFVG
CHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCCCCEEEEEEECCCHHHHHHHHHHHHHHH
LHFGGADKQLALQNALSGFWGTIFWVGVFFIGILIPLVANIFGSHSLKHNVKFIILVSIF
HCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHCCCHHHHHHHHH
DLIGVLCLRYFILYAGQLTVAS
HHHHHHHHHHHHHHHCCCEECC
>Mature Secondary Structure
MNSYIPFQTPNLVWDSTIAIYLFLLGISSGAVQLAIAYRNSGAKIAKNSDNWVIRSAAIL
CCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCEEEEEEEECCCCEEEECCCCEEEEHHHHH
GTLPTLIGLTLLIFHLTKPWTFWKLMFNYNGTSVMSMGVMLFQVYMAVLVVWIAIMFKDW
HHHHHHHHHHHHHHHHCCCCEEEHEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LAVLVNRYLPMFKFVLPLVDFAETRLLKAIEFVLFVLAAVLGAYPGFLLSALISYPMLNN
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHCCCCCC
PVLPALFLASGTSSGIAATFLVILLAGKLSGESNEVHFMHKFEVPIMVTELGLLVAFFVG
CHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCCCCEEEEEEECCCHHHHHHHHHHHHHHH
LHFGGADKQLALQNALSGFWGTIFWVGVFFIGILIPLVANIFGSHSLKHNVKFIILVSIF
HCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHCCCHHHHHHHHH
DLIGVLCLRYFILYAGQLTVAS
HHHHHHHHHHHHHHHCCCEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: reduced cytochrome C552; nitrite [C]

Specific reaction: reduced cytochrome C552 + nitrite = oxidized cytochrome C552 + NH3 [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]