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The map label for this gene is murE

Identifier: 126207501

GI number: 126207501

Start: 13754

End: 15256

Strand: Direct

Name: murE

Synonym: APL_0013

Alternate gene names: 126207501

Gene position: 13754-15256 (Clockwise)

Preceding gene: 126207500

Following gene: 126207502

Centisome position: 0.6

GC content: 45.38

Gene sequence:

>1503_bases
ATGAAACGTTTACTTCCCTTTCTCACCGAACTTGATGCTTGGGTGACGGAATTAAAACCGCTTAAGCAAATGACGTTGGA
TTCTCGCCAAGTTGATGAAGGCGATCTGTTTGTGGCGTTAAAAGGGCATCAAGTGGATGGTCGCCGATTTATTCAAAAAG
CGATTGAGCAAGGCGCAGCTTTAGTCTTGGCGGAAGCGGACGAAGGGCAGGACGAAATTGAGTTGGATGCGAAATTTGCA
AAATATAACTTAGATCGCACCGCTTGTAAGGTGGTTTTAGTACCGAATTTGGCTCGTATTTTATCGGAAATTGCCGGTGC
GTTTTATGCCAATCCGTCTGAAAAACTCACTCTCGTAGGTGTAACCGGTACCAACGGCAAGACCACAAGCGCGCAATTAC
TTGCCCAATGGCATAACCTATTAGGCGGAAAATCGGCGGTAATGGGGACGATCGGTAACGGTTTATACGGCAAAGTACAA
GAAGCGGCAAATACCACCGGTTCGGCGATTGAAGTGCAACGTAATTTAGCCGACTTTGTGGAAATGGGCGCTGATTTTTG
TGCGATGGAAGTTTCTTCGCACGGCTTAGCTCAATATCGTGCGGAAGCGTTAAGCTATGATTTAGCGGTATTCACTAATT
TAAGCCGTGATCATCTCGATTACCACAAGACAATGGAAGAATATGCACAAGCAAAATTCCGTTTGTTTAGCGAATTAGAT
ACCAAAGCACAAGTATTAAACGCTGATGATGAAGTCGGGCGTGAATGGTTGGCAAAATTGCCAGATGCGGTTGCCGTCAG
TACCGATGCGAGCTTTAGTAGCGAGCATAAATTCGTTAAAGCGACCGATGTGAGCTTTAGCTTACAAGGTGTAAAAATTG
CGTTTGAATCGAGCTGGGGCAACGGCGAGTTAAATAGCCGTTTAATCGGTGCATTTAACGTCAATAATTTACTCACAGCA
TTAGCCGGCTTATTAGTGCTTGGTCATGATTTACCAAAATTAATTGAAACTGCACCGCTTCTGCAAGGGGTTGCCGGACG
TATGGAGTGTGTCGTTTCGCAAAAAAATCCGCAAAATAGACCGCTTGTATTAGTCGATTACGCTCATACGCCGGATGCGT
TGGAAAAAGCCTTGCAAGCGGCACGATTACATACCGAAGGCGAGCTTTATTGTATTTTCGGTTGCGGTGGTGATCGTGAT
GCGGGCAAACGCCCGATGATGGCGGCGATTGCCGAAGAACTGGCGGATAAAGTGATTGCGACCGATGACAATCCGAGAAC
CGAAGATAACGCTAAGATTATGGCGGATATTCTCAACGGTTTCGTTCAAGTGGAGAAAGTACAAGTGATCCACGATCGTG
AACAAGCAATTAAAACCGCAATTGAACAAGCGAATGAAAAAGATGTGATTTTAATTGCCGGTAAAGGGCACGAAGATTAC
CAAATTATCGGGACGACTAAGCATCATTTTTCGGATCAGGAAACCGCGGCTAAATACCTGTAG

Upstream 100 bases:

>100_bases
GAAATATCAAACCGGATAACCTCACCGACACCGAAAGTGCCGTACGAGAAATTAAATTAGAACAAAAAGTAAATTAGAAC
AGAATAAATTAGGTAACAAT

Downstream 100 bases:

>100_bases
CTTGTCACTTTCTTTGCTTCGCCAAAGAAAGTAACCAAAGAAAGGCGACCCCAGATTTTCCTTTTTCCTCTGCTTATTGC
TAACTTTTGGGACGAATTTT

Product: UDP-N-acetylmuramoylalanyl-D-glutamate--2, 6-diaminopimelate ligase

Products: NA

Alternate protein names: Meso-A2pm-adding enzyme; Meso-diaminopimelate-adding enzyme; UDP-MurNAc-L-Ala-D-Glu:meso-diaminopimelate ligase; UDP-MurNAc-tripeptide synthetase; UDP-N-acetylmuramyl-tripeptide synthetase

Number of amino acids: Translated: 500; Mature: 500

Protein sequence:

>500_residues
MKRLLPFLTELDAWVTELKPLKQMTLDSRQVDEGDLFVALKGHQVDGRRFIQKAIEQGAALVLAEADEGQDEIELDAKFA
KYNLDRTACKVVLVPNLARILSEIAGAFYANPSEKLTLVGVTGTNGKTTSAQLLAQWHNLLGGKSAVMGTIGNGLYGKVQ
EAANTTGSAIEVQRNLADFVEMGADFCAMEVSSHGLAQYRAEALSYDLAVFTNLSRDHLDYHKTMEEYAQAKFRLFSELD
TKAQVLNADDEVGREWLAKLPDAVAVSTDASFSSEHKFVKATDVSFSLQGVKIAFESSWGNGELNSRLIGAFNVNNLLTA
LAGLLVLGHDLPKLIETAPLLQGVAGRMECVVSQKNPQNRPLVLVDYAHTPDALEKALQAARLHTEGELYCIFGCGGDRD
AGKRPMMAAIAEELADKVIATDDNPRTEDNAKIMADILNGFVQVEKVQVIHDREQAIKTAIEQANEKDVILIAGKGHEDY
QIIGTTKHHFSDQETAAKYL

Sequences:

>Translated_500_residues
MKRLLPFLTELDAWVTELKPLKQMTLDSRQVDEGDLFVALKGHQVDGRRFIQKAIEQGAALVLAEADEGQDEIELDAKFA
KYNLDRTACKVVLVPNLARILSEIAGAFYANPSEKLTLVGVTGTNGKTTSAQLLAQWHNLLGGKSAVMGTIGNGLYGKVQ
EAANTTGSAIEVQRNLADFVEMGADFCAMEVSSHGLAQYRAEALSYDLAVFTNLSRDHLDYHKTMEEYAQAKFRLFSELD
TKAQVLNADDEVGREWLAKLPDAVAVSTDASFSSEHKFVKATDVSFSLQGVKIAFESSWGNGELNSRLIGAFNVNNLLTA
LAGLLVLGHDLPKLIETAPLLQGVAGRMECVVSQKNPQNRPLVLVDYAHTPDALEKALQAARLHTEGELYCIFGCGGDRD
AGKRPMMAAIAEELADKVIATDDNPRTEDNAKIMADILNGFVQVEKVQVIHDREQAIKTAIEQANEKDVILIAGKGHEDY
QIIGTTKHHFSDQETAAKYL
>Mature_500_residues
MKRLLPFLTELDAWVTELKPLKQMTLDSRQVDEGDLFVALKGHQVDGRRFIQKAIEQGAALVLAEADEGQDEIELDAKFA
KYNLDRTACKVVLVPNLARILSEIAGAFYANPSEKLTLVGVTGTNGKTTSAQLLAQWHNLLGGKSAVMGTIGNGLYGKVQ
EAANTTGSAIEVQRNLADFVEMGADFCAMEVSSHGLAQYRAEALSYDLAVFTNLSRDHLDYHKTMEEYAQAKFRLFSELD
TKAQVLNADDEVGREWLAKLPDAVAVSTDASFSSEHKFVKATDVSFSLQGVKIAFESSWGNGELNSRLIGAFNVNNLLTA
LAGLLVLGHDLPKLIETAPLLQGVAGRMECVVSQKNPQNRPLVLVDYAHTPDALEKALQAARLHTEGELYCIFGCGGDRD
AGKRPMMAAIAEELADKVIATDDNPRTEDNAKIMADILNGFVQVEKVQVIHDREQAIKTAIEQANEKDVILIAGKGHEDY
QIIGTTKHHFSDQETAAKYL

Specific function: Catalyzes the addition of meso-diaminopimelic acid to the nucleotide precursor UDP-N-acetylmuramoyl-L-alanyl-D-glutamate (UMAG) in the biosynthesis of bacterial cell-wall peptidoglycan

COG id: COG0769

COG function: function code M; UDP-N-acetylmuramyl tripeptide synthase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MurCDEF family. MurE subfamily

Homologues:

Organism=Escherichia coli, GI1786273, Length=496, Percent_Identity=55.6451612903226, Blast_Score=488, Evalue=1e-139,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MURE_ACTP2 (A3MY85)

Other databases:

- EMBL:   CP000569
- RefSeq:   YP_001052726.1
- ProteinModelPortal:   A3MY85
- SMR:   A3MY85
- STRING:   A3MY85
- GeneID:   4849091
- GenomeReviews:   CP000569_GR
- KEGG:   apl:APL_0013
- eggNOG:   COG0769
- HOGENOM:   HBG602753
- OMA:   GHEEGQT
- PhylomeDB:   A3MY85
- ProtClustDB:   PRK00139
- BioCyc:   APLE416269:APL_0013-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00208
- InterPro:   IPR004101
- InterPro:   IPR013221
- InterPro:   IPR000713
- InterPro:   IPR005761
- Gene3D:   G3DSA:3.90.190.20
- Gene3D:   G3DSA:3.40.1190.10
- TIGRFAMs:   TIGR01085

Pfam domain/function: PF01225 Mur_ligase; PF02875 Mur_ligase_C; PF08245 Mur_ligase_M; SSF53244 Mur_ligase_C; SSF53623 Mur_ligase_cen

EC number: =6.3.2.13

Molecular weight: Translated: 54630; Mature: 54630

Theoretical pI: Translated: 4.89; Mature: 4.89

Prosite motif: NA

Important sites: BINDING 26-26 BINDING 28-28 BINDING 164-164 BINDING 192-192 BINDING 198-198 BINDING 200-200 BINDING 399-399 BINDING 474-474 BINDING 478-478

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKRLLPFLTELDAWVTELKPLKQMTLDSRQVDEGDLFVALKGHQVDGRRFIQKAIEQGAA
CCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEECCCCCHHHHHHHHHHCCCE
LVLAEADEGQDEIELDAKFAKYNLDRTACKVVLVPNLARILSEIAGAFYANPSEKLTLVG
EEEEECCCCCCCEEECHHHHHCCCCHHHEEEEECCCHHHHHHHHHHHHCCCCCCCEEEEE
VTGTNGKTTSAQLLAQWHNLLGGKSAVMGTIGNGLYGKVQEAANTTGSAIEVQRNLADFV
EECCCCCCHHHHHHHHHHHHHCCCHHHHHHCCCCCHHHHHHHCCCCCCHHHHHHHHHHHH
EMGADFCAMEVSSHGLAQYRAEALSYDLAVFTNLSRDHLDYHKTMEEYAQAKFRLFSELD
HCCCHHEEHHHHCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHC
TKAQVLNADDEVGREWLAKLPDAVAVSTDASFSSEHKFVKATDVSFSLQGVKIAFESSWG
CHHHHCCCCHHHHHHHHHHCCCCEEEECCCCCCCCCCEEEEECCEEEEEEEEEEEECCCC
NGELNSRLIGAFNVNNLLTALAGLLVLGHDLPKLIETAPLLQGVAGRMECVVSQKNPQNR
CCCCCCEEEEEECHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHEEEEECCCCCCC
PLVLVDYAHTPDALEKALQAARLHTEGELYCIFGCGGDRDAGKRPMMAAIAEELADKVIA
CEEEEEECCCCHHHHHHHHHHHCCCCCCEEEEEECCCCCCCCCCHHHHHHHHHHHHHEEE
TDDNPRTEDNAKIMADILNGFVQVEKVQVIHDREQAIKTAIEQANEKDVILIAGKGHEDY
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCE
QIIGTTKHHFSDQETAAKYL
EEEECCHHHCCCHHHHHHCC
>Mature Secondary Structure
MKRLLPFLTELDAWVTELKPLKQMTLDSRQVDEGDLFVALKGHQVDGRRFIQKAIEQGAA
CCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEECCCCCHHHHHHHHHHCCCE
LVLAEADEGQDEIELDAKFAKYNLDRTACKVVLVPNLARILSEIAGAFYANPSEKLTLVG
EEEEECCCCCCCEEECHHHHHCCCCHHHEEEEECCCHHHHHHHHHHHHCCCCCCCEEEEE
VTGTNGKTTSAQLLAQWHNLLGGKSAVMGTIGNGLYGKVQEAANTTGSAIEVQRNLADFV
EECCCCCCHHHHHHHHHHHHHCCCHHHHHHCCCCCHHHHHHHCCCCCCHHHHHHHHHHHH
EMGADFCAMEVSSHGLAQYRAEALSYDLAVFTNLSRDHLDYHKTMEEYAQAKFRLFSELD
HCCCHHEEHHHHCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHC
TKAQVLNADDEVGREWLAKLPDAVAVSTDASFSSEHKFVKATDVSFSLQGVKIAFESSWG
CHHHHCCCCHHHHHHHHHHCCCCEEEECCCCCCCCCCEEEEECCEEEEEEEEEEEECCCC
NGELNSRLIGAFNVNNLLTALAGLLVLGHDLPKLIETAPLLQGVAGRMECVVSQKNPQNR
CCCCCCEEEEEECHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHEEEEECCCCCCC
PLVLVDYAHTPDALEKALQAARLHTEGELYCIFGCGGDRDAGKRPMMAAIAEELADKVIA
CEEEEEECCCCHHHHHHHHHHHCCCCCCEEEEEECCCCCCCCCCHHHHHHHHHHHHHEEE
TDDNPRTEDNAKIMADILNGFVQVEKVQVIHDREQAIKTAIEQANEKDVILIAGKGHEDY
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCE
QIIGTTKHHFSDQETAAKYL
EEEECCHHHCCCHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA