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The map label for this gene is murF [H]

Identifier: 126207502

GI number: 126207502

Start: 15471

End: 16865

Strand: Direct

Name: murF [H]

Synonym: APL_0014

Alternate gene names: 126207502

Gene position: 15471-16865 (Clockwise)

Preceding gene: 126207501

Following gene: 126207503

Centisome position: 0.68

GC content: 44.73

Gene sequence:

>1395_bases
ATGATAAAACTAACAACAAATGAAATTGCCGGCATTCTTAATGCACAGCTAATTGGTGATGGCAATGTGCTGGTGGAAAC
CACTAGCACCGATACTCGACAAGCGGTCGAAAACGGGCTGTTTTTTGCGTTAAAAGGCGAGAACTTTGATGCGCACCATT
ACTTAGCTAATGCGGTGGAACAAGGCAGTGTGGCAGTAGTGGTTGAGCGTGAATGTGAGATTTCCGTGCCGCAAATTGTG
GTGAAAGATACACGCCTTGCGTTAGGCGAGTTAGCCAAATGGTTAAAAGCGAAACTTAATCCGAAAACCGTGGCGATGAC
CGGTTCTTCAGGCAAAACCACCGTAAAAGAAATGACGGCAAAAATTTTGCAAAAAATGACCGCTTGTGAAGATGAAGTGC
TTTATACCTTTGGCAACCTAAATAACGACCTCGGTGTGCCGATGACCTTGTTACGTTTAACGCCGCAACATAAATTTGCG
GTGGTGGAACTCGGGGCAAACCATATCGGCGAAATCGCTTATACCACAGAAATTACTCAGCCGGACGCTTGTTTAGTGAA
TAACGTTGCCGCGGCGCATTTAGAAGGTTTCGGCTCACTCGAAGGTGTAGCGCAAGCAAAAGGCGAAATTTATCGAGGCT
TAAAAGCCGGTGGTAAAGCGATTGTGAATCAAGCCTTTTATTATCCGCAATGGCAAAAAGAAATCGGCGAGCATGAGCTA
CAATCATTTAGCTATATCGGTTCGGATCAAGATTCGTATGCGGATTTTTGGGCGGAAAATGTGGAATTGGGTTTAGCCGG
ATCTCGCTTTACCTTACATTCTCCGCAAGGTGAAATCGAAATTAATTTGCCTTACTTAGGCGCACATAATATCAGCAACG
CTTTAGCAGCAACTTCGCTCTCTATGGCGGTCGGTGCGGATTTACAAGCGGTCAAATCCGGTTTGGAACAACGTTCGCAA
GTAAAAGGTCGTTTGTATCCGGTTGAAGTCAATGATCATTGTTTGTTCATTGACGATACTTATAACGCCAATGTCGATTC
GATGAAATCGGCGGTGTCGGTGTTAAAAAATTATCCGGCTTTCCGCATTTTTGCGGTCGGCGATATGGCGGAACTCGGTG
GCGAAAGTGAGGCTTGTCATCAGCAAGTGGCGGATTTCGTACGTGAAGCGAATTTAGATTTAGTCGTCAGCTTCGGTAAG
CAAAGTGCGGTAATCAGTCATGATACGGCACATCATTTTACCGATAAACAAGCGATGCACGATTTCTTATTGCCGATTAT
TTCGCAAAAAATTGCGGAAAAACAACCGCTTGTGTTATTGGCAAAAGGCTCTCGCAGCCAAAAAATGGAAACGTTGATTT
CCGCTTTATGCCTTTCTTTGGGCGTTTCTTTTTAA

Upstream 100 bases:

>100_bases
AAGCAAGCTCAGACACAAAAAATTGTCCTCAAAAGTTTACGCAACCGCTTCGGAGGAAAATAGGGGGAAATGCAATAAAT
AGACTGAAGATAGATATTCA

Downstream 100 bases:

>100_bases
TGTTAAGGTAGATAGCGGTGCGAGCGTTTGCTCGTCGCTATTTAAATATAAATCGGGTGATTTACCCGCTTAAAATAATT
TAACAAGGGTTTTGTTATGT

Product: UDP-MurNAc-pentapeptide synthetase

Products: NA

Alternate protein names: D-alanyl-D-alanine-adding enzyme; UDP-MurNAc-pentapeptide synthetase [H]

Number of amino acids: Translated: 464; Mature: 464

Protein sequence:

>464_residues
MIKLTTNEIAGILNAQLIGDGNVLVETTSTDTRQAVENGLFFALKGENFDAHHYLANAVEQGSVAVVVERECEISVPQIV
VKDTRLALGELAKWLKAKLNPKTVAMTGSSGKTTVKEMTAKILQKMTACEDEVLYTFGNLNNDLGVPMTLLRLTPQHKFA
VVELGANHIGEIAYTTEITQPDACLVNNVAAAHLEGFGSLEGVAQAKGEIYRGLKAGGKAIVNQAFYYPQWQKEIGEHEL
QSFSYIGSDQDSYADFWAENVELGLAGSRFTLHSPQGEIEINLPYLGAHNISNALAATSLSMAVGADLQAVKSGLEQRSQ
VKGRLYPVEVNDHCLFIDDTYNANVDSMKSAVSVLKNYPAFRIFAVGDMAELGGESEACHQQVADFVREANLDLVVSFGK
QSAVISHDTAHHFTDKQAMHDFLLPIISQKIAEKQPLVLLAKGSRSQKMETLISALCLSLGVSF

Sequences:

>Translated_464_residues
MIKLTTNEIAGILNAQLIGDGNVLVETTSTDTRQAVENGLFFALKGENFDAHHYLANAVEQGSVAVVVERECEISVPQIV
VKDTRLALGELAKWLKAKLNPKTVAMTGSSGKTTVKEMTAKILQKMTACEDEVLYTFGNLNNDLGVPMTLLRLTPQHKFA
VVELGANHIGEIAYTTEITQPDACLVNNVAAAHLEGFGSLEGVAQAKGEIYRGLKAGGKAIVNQAFYYPQWQKEIGEHEL
QSFSYIGSDQDSYADFWAENVELGLAGSRFTLHSPQGEIEINLPYLGAHNISNALAATSLSMAVGADLQAVKSGLEQRSQ
VKGRLYPVEVNDHCLFIDDTYNANVDSMKSAVSVLKNYPAFRIFAVGDMAELGGESEACHQQVADFVREANLDLVVSFGK
QSAVISHDTAHHFTDKQAMHDFLLPIISQKIAEKQPLVLLAKGSRSQKMETLISALCLSLGVSF
>Mature_464_residues
MIKLTTNEIAGILNAQLIGDGNVLVETTSTDTRQAVENGLFFALKGENFDAHHYLANAVEQGSVAVVVERECEISVPQIV
VKDTRLALGELAKWLKAKLNPKTVAMTGSSGKTTVKEMTAKILQKMTACEDEVLYTFGNLNNDLGVPMTLLRLTPQHKFA
VVELGANHIGEIAYTTEITQPDACLVNNVAAAHLEGFGSLEGVAQAKGEIYRGLKAGGKAIVNQAFYYPQWQKEIGEHEL
QSFSYIGSDQDSYADFWAENVELGLAGSRFTLHSPQGEIEINLPYLGAHNISNALAATSLSMAVGADLQAVKSGLEQRSQ
VKGRLYPVEVNDHCLFIDDTYNANVDSMKSAVSVLKNYPAFRIFAVGDMAELGGESEACHQQVADFVREANLDLVVSFGK
QSAVISHDTAHHFTDKQAMHDFLLPIISQKIAEKQPLVLLAKGSRSQKMETLISALCLSLGVSF

Specific function: Involved in cell wall formation. Catalyzes the final step in the synthesis of UDP-N-acetylmuramoyl-pentapeptide, the precursor of murein [H]

COG id: COG0770

COG function: function code M; UDP-N-acetylmuramyl pentapeptide synthase

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MurCDEF family [H]

Homologues:

Organism=Escherichia coli, GI1786274, Length=459, Percent_Identity=50.3267973856209, Blast_Score=407, Evalue=1e-115,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004101
- InterPro:   IPR013221
- InterPro:   IPR000713
- InterPro:   IPR005863 [H]

Pfam domain/function: PF01225 Mur_ligase; PF02875 Mur_ligase_C; PF08245 Mur_ligase_M [H]

EC number: =6.3.2.10 [H]

Molecular weight: Translated: 50347; Mature: 50347

Theoretical pI: Translated: 5.18; Mature: 5.18

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIKLTTNEIAGILNAQLIGDGNVLVETTSTDTRQAVENGLFFALKGENFDAHHYLANAVE
CEEEEHHHHHHHHEEEEEECCCEEEEECCCHHHHHHHCCCEEEEECCCCCHHHHHHHHHC
QGSVAVVVERECEISVPQIVVKDTRLALGELAKWLKAKLNPKTVAMTGSSGKTTVKEMTA
CCCEEEEEECCCCCCCCEEEEEHHHHHHHHHHHHHHHCCCCCEEEEECCCCCHHHHHHHH
KILQKMTACEDEVLYTFGNLNNDLGVPMTLLRLTPQHKFAVVELGANHIGEIAYTTEITQ
HHHHHHHCCCHHEEEEECCCCCCCCCCEEEEEECCCCCEEEEEECCCCCCCEEEEEECCC
PDACLVNNVAAAHLEGFGSLEGVAQAKGEIYRGLKAGGKAIVNQAFYYPQWQKEIGEHEL
CCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCCHHHHHHHHHH
QSFSYIGSDQDSYADFWAENVELGLAGSRFTLHSPQGEIEINLPYLGAHNISNALAATSL
HHHHHCCCCCCHHHHHHHCCCEEEECCCEEEEECCCCCEEEECCCCCCCCHHHHHHHHHH
SMAVGADLQAVKSGLEQRSQVKGRLYPVEVNDHCLFIDDTYNANVDSMKSAVSVLKNYPA
HHHHCCCHHHHHHHHHHHHHHCCCEEEEEECCEEEEEECCCCCCHHHHHHHHHHHHCCCC
FRIFAVGDMAELGGESEACHQQVADFVREANLDLVVSFGKQSAVISHDTAHHFTDKQAMH
EEEEEECCHHHHCCCHHHHHHHHHHHHHHCCCEEEEEECCCCEEEECCCHHHHCHHHHHH
DFLLPIISQKIAEKQPLVLLAKGSRSQKMETLISALCLSLGVSF
HHHHHHHHHHHCCCCCEEEEECCCCHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MIKLTTNEIAGILNAQLIGDGNVLVETTSTDTRQAVENGLFFALKGENFDAHHYLANAVE
CEEEEHHHHHHHHEEEEEECCCEEEEECCCHHHHHHHCCCEEEEECCCCCHHHHHHHHHC
QGSVAVVVERECEISVPQIVVKDTRLALGELAKWLKAKLNPKTVAMTGSSGKTTVKEMTA
CCCEEEEEECCCCCCCCEEEEEHHHHHHHHHHHHHHHCCCCCEEEEECCCCCHHHHHHHH
KILQKMTACEDEVLYTFGNLNNDLGVPMTLLRLTPQHKFAVVELGANHIGEIAYTTEITQ
HHHHHHHCCCHHEEEEECCCCCCCCCCEEEEEECCCCCEEEEEECCCCCCCEEEEEECCC
PDACLVNNVAAAHLEGFGSLEGVAQAKGEIYRGLKAGGKAIVNQAFYYPQWQKEIGEHEL
CCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCCHHHHHHHHHH
QSFSYIGSDQDSYADFWAENVELGLAGSRFTLHSPQGEIEINLPYLGAHNISNALAATSL
HHHHHCCCCCCHHHHHHHCCCEEEECCCEEEEECCCCCEEEECCCCCCCCHHHHHHHHHH
SMAVGADLQAVKSGLEQRSQVKGRLYPVEVNDHCLFIDDTYNANVDSMKSAVSVLKNYPA
HHHHCCCHHHHHHHHHHHHHHCCCEEEEEECCEEEEEECCCCCCHHHHHHHHHHHHCCCC
FRIFAVGDMAELGGESEACHQQVADFVREANLDLVVSFGKQSAVISHDTAHHFTDKQAMH
EEEEEECCHHHHCCCHHHHHHHHHHHHHHCCCEEEEEECCCCEEEECCCHHHHCHHHHHH
DFLLPIISQKIAEKQPLVLLAKGSRSQKMETLISALCLSLGVSF
HHHHHHHHHHHCCCCCEEEEECCCCHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]