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The map label for this gene is rpfG [H]

Identifier: 126174234

GI number: 126174234

Start: 2332166

End: 2333761

Strand: Direct

Name: rpfG [H]

Synonym: Sbal_2012

Alternate gene names: 126174234

Gene position: 2332166-2333761 (Clockwise)

Preceding gene: 126174233

Following gene: 126174235

Centisome position: 45.48

GC content: 43.92

Gene sequence:

>1596_bases
ATGAGTGATTTTCTCAATGATTGGCTAACCGAAGACACCTGTGAAACAAAAGAAAACAACCACCAACGAGTGTGGAATGT
ATTAGTGGTAGATGATGAGCCTGATATCGTCAACGTTACCAGATTAACGCTAAACAGCTTCACCTATAAAAACAAGACAC
TTAATGTACAACACGCCTACTCTGCTAGCGAGGCGATCGAAATCATAAGCCACTCAACTGACCTCGCACTTATATTACTT
GATGTGGTGATGGAGACGGACGACGCCGGCCTCAAGCTAGTGAAATGGATCAGAAATGAATTAGGTAATCACATGGTACG
TATCGTGTTACGTACTGGCCAACCTGGCTATGCTCCTGAAAAAAGCGTTATCGTTGACTACGACATTAATGACTATAAAT
TAAAAACAGAATTAACAGCGGAAAAACTATTCACTGTGGTGATCGCTTCGCTGCGCGCCTATGACAGTCTTTGGTCTCTC
GAGGAAAACCGCATCGGCCTGATGAAAATTTTAGAGGGCACATCGAGCCTGTACCGCATTTCATCTCTGAAACAGTTTGC
CTCAGGTATACTTGATCAAATTAGTTTACTGCTAAAGTTCCCCCCTGAAGGCATCCTGGTGATGGCCAAAGGCATGCAAC
CAGATACACCAGAACGGTTAACCATAATAGCTACCACTGGACTGTATGAAACGCTTGAGGCCGATGAGCAAGGTCTTATT
AATCAGCCTGAGTTAACCCACAACATAGCATTAGCCCACCAAAACCAAAAATCCGTTTTTCTACACCCTCACGACGTGCT
TTGCATCCCCGCACACAACGGACACATATTCTTTATATACTTCTCCCCCATATATGAAATTGACGCTGCAGAACAAGCAT
TGCTTGAAATATTTTGTGAGCGCATGTCAGCTGCCTACGACAATTATCATTTATTTGGTCAGCTTAAAAAATCACAGGAA
GCCAGCGTTGTAGCCATGGCGAGCTTAGCCGAACATAAGGATGAAGACACAGGATTGCATGTGTTACGCGTACGTGATTT
TACTGAGGCAATTGCTAATGAACTATTGCAAAATGGACATTTTACAGACGAGCTGACTCAGGGCTTTTTATCAAAAGTAG
GTTGGGCGAGTATTCTCCACGACATAGGTAAAATAGGCATTCCAGATTGCATCTTGCAAAAAAAAGGCAAACTAGATAGT
GACGAGCGCACCATCATGGAAACACATGCCCGCATGGGTGAAGATATTCTATTTAAAGCATCCGAATTGATACAAGGCAG
AAATTATTTTTCTATCGCTGCTGACATTGCCGGCGGCCATCATGAATACTTTGACGGCAACGGCTATCCAAGAGGATTAT
CTGGAGAGCAAATCCCACTCAGTGCACGCATTGTAGCTGTGGTTGATGTATTTGATGCCTTAATGAGTGAGCGCCCATAC
AAAACCGCTTGGCCCCTTAATCAGGCAATAGACTATATCCTCGGTCGCAGCAGCAGCCAGTTTGATCCTCTTGTGGTGCA
AGCTATTTTAGCTTTAGTCACAGAAAAACGACTCCCGCCAGTGTTGTGTGAATACTATGGCCTTGGCAAACATTGA

Upstream 100 bases:

>100_bases
GTCACGGATAAACCCAAATAGGACCTCTACCGAACTCAATACATCAGATTAGGCAATAACTATCAAACTCAAACTACTCG
TAAGTAATGAGGCTTTCCAC

Downstream 100 bases:

>100_bases
TTGGATATCCACAATGAAAAAGCCCCATGCATTGAGCGTATTCTATGCCACATTGGCTGCCGCATTTTTATTACTTTTGG
CCATGTCTATAACGTTCTAT

Product: response regulator receiver modulated metal dependent phosphohydrolase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 531; Mature: 530

Protein sequence:

>531_residues
MSDFLNDWLTEDTCETKENNHQRVWNVLVVDDEPDIVNVTRLTLNSFTYKNKTLNVQHAYSASEAIEIISHSTDLALILL
DVVMETDDAGLKLVKWIRNELGNHMVRIVLRTGQPGYAPEKSVIVDYDINDYKLKTELTAEKLFTVVIASLRAYDSLWSL
EENRIGLMKILEGTSSLYRISSLKQFASGILDQISLLLKFPPEGILVMAKGMQPDTPERLTIIATTGLYETLEADEQGLI
NQPELTHNIALAHQNQKSVFLHPHDVLCIPAHNGHIFFIYFSPIYEIDAAEQALLEIFCERMSAAYDNYHLFGQLKKSQE
ASVVAMASLAEHKDEDTGLHVLRVRDFTEAIANELLQNGHFTDELTQGFLSKVGWASILHDIGKIGIPDCILQKKGKLDS
DERTIMETHARMGEDILFKASELIQGRNYFSIAADIAGGHHEYFDGNGYPRGLSGEQIPLSARIVAVVDVFDALMSERPY
KTAWPLNQAIDYILGRSSSQFDPLVVQAILALVTEKRLPPVLCEYYGLGKH

Sequences:

>Translated_531_residues
MSDFLNDWLTEDTCETKENNHQRVWNVLVVDDEPDIVNVTRLTLNSFTYKNKTLNVQHAYSASEAIEIISHSTDLALILL
DVVMETDDAGLKLVKWIRNELGNHMVRIVLRTGQPGYAPEKSVIVDYDINDYKLKTELTAEKLFTVVIASLRAYDSLWSL
EENRIGLMKILEGTSSLYRISSLKQFASGILDQISLLLKFPPEGILVMAKGMQPDTPERLTIIATTGLYETLEADEQGLI
NQPELTHNIALAHQNQKSVFLHPHDVLCIPAHNGHIFFIYFSPIYEIDAAEQALLEIFCERMSAAYDNYHLFGQLKKSQE
ASVVAMASLAEHKDEDTGLHVLRVRDFTEAIANELLQNGHFTDELTQGFLSKVGWASILHDIGKIGIPDCILQKKGKLDS
DERTIMETHARMGEDILFKASELIQGRNYFSIAADIAGGHHEYFDGNGYPRGLSGEQIPLSARIVAVVDVFDALMSERPY
KTAWPLNQAIDYILGRSSSQFDPLVVQAILALVTEKRLPPVLCEYYGLGKH
>Mature_530_residues
SDFLNDWLTEDTCETKENNHQRVWNVLVVDDEPDIVNVTRLTLNSFTYKNKTLNVQHAYSASEAIEIISHSTDLALILLD
VVMETDDAGLKLVKWIRNELGNHMVRIVLRTGQPGYAPEKSVIVDYDINDYKLKTELTAEKLFTVVIASLRAYDSLWSLE
ENRIGLMKILEGTSSLYRISSLKQFASGILDQISLLLKFPPEGILVMAKGMQPDTPERLTIIATTGLYETLEADEQGLIN
QPELTHNIALAHQNQKSVFLHPHDVLCIPAHNGHIFFIYFSPIYEIDAAEQALLEIFCERMSAAYDNYHLFGQLKKSQEA
SVVAMASLAEHKDEDTGLHVLRVRDFTEAIANELLQNGHFTDELTQGFLSKVGWASILHDIGKIGIPDCILQKKGKLDSD
ERTIMETHARMGEDILFKASELIQGRNYFSIAADIAGGHHEYFDGNGYPRGLSGEQIPLSARIVAVVDVFDALMSERPYK
TAWPLNQAIDYILGRSSSQFDPLVVQAILALVTEKRLPPVLCEYYGLGKH

Specific function: Positively regulates the synthesis of extracellular enzymes and extracellular polysaccharides. The cell-cell signaling system encoded by rpf genes is required for full virulence and is implicated in regulation of the formation and dispersal of biofilms [H

COG id: COG3437

COG function: function code KT; Response regulator containing a CheY-like receiver domain and an HD-GYP domain

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Contains 1 response regulatory domain [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011006
- InterPro:   IPR003607
- InterPro:   IPR001789 [H]

Pfam domain/function: PF00072 Response_reg [H]

EC number: NA

Molecular weight: Translated: 59647; Mature: 59516

Theoretical pI: Translated: 4.85; Mature: 4.85

Prosite motif: PS50110 RESPONSE_REGULATORY

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSDFLNDWLTEDTCETKENNHQRVWNVLVVDDEPDIVNVTRLTLNSFTYKNKTLNVQHAY
CCHHHHHHHCCCCHHCCCCCCEEEEEEEEECCCCCEEEEEEEEECCEEECCCEEEEEECC
SASEAIEIISHSTDLALILLDVVMETDDAGLKLVKWIRNELGNHMVRIVLRTGQPGYAPE
CHHHHHHHHHCCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCEEEEEEECCCCCCCCC
KSVIVDYDINDYKLKTELTAEKLFTVVIASLRAYDSLWSLEENRIGLMKILEGTSSLYRI
CCEEEEECCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHCCHHHHHH
SSLKQFASGILDQISLLLKFPPEGILVMAKGMQPDTPERLTIIATTGLYETLEADEQGLI
HHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCCCEEEEEECCCHHHHCCCCCCCC
NQPELTHNIALAHQNQKSVFLHPHDVLCIPAHNGHIFFIYFSPIYEIDAAEQALLEIFCE
CCCCCHHEEEEEECCCCEEEECCCCEEEEECCCCEEEEEEECCEEECCHHHHHHHHHHHH
RMSAAYDNYHLFGQLKKSQEASVVAMASLAEHKDEDTGLHVLRVRDFTEAIANELLQNGH
HHHHHCCCCCEEHHHHCCCCCHHHHHHHHHHCCCCCCCEEEEEEHHHHHHHHHHHHHCCC
FTDELTQGFLSKVGWASILHDIGKIGIPDCILQKKGKLDSDERTIMETHARMGEDILFKA
CHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHCCCCCCCCHHHHHHHHHHCCHHHHHHH
SELIQGRNYFSIAADIAGGHHEYFDGNGYPRGLSGEQIPLSARIVAVVDVFDALMSERPY
HHHHCCCCEEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCC
KTAWPLNQAIDYILGRSSSQFDPLVVQAILALVTEKRLPPVLCEYYGLGKH
CCCCCHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHCCCCHHHHHHCCCCCC
>Mature Secondary Structure 
SDFLNDWLTEDTCETKENNHQRVWNVLVVDDEPDIVNVTRLTLNSFTYKNKTLNVQHAY
CHHHHHHHCCCCHHCCCCCCEEEEEEEEECCCCCEEEEEEEEECCEEECCCEEEEEECC
SASEAIEIISHSTDLALILLDVVMETDDAGLKLVKWIRNELGNHMVRIVLRTGQPGYAPE
CHHHHHHHHHCCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCEEEEEEECCCCCCCCC
KSVIVDYDINDYKLKTELTAEKLFTVVIASLRAYDSLWSLEENRIGLMKILEGTSSLYRI
CCEEEEECCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHCCHHHHHH
SSLKQFASGILDQISLLLKFPPEGILVMAKGMQPDTPERLTIIATTGLYETLEADEQGLI
HHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCCCEEEEEECCCHHHHCCCCCCCC
NQPELTHNIALAHQNQKSVFLHPHDVLCIPAHNGHIFFIYFSPIYEIDAAEQALLEIFCE
CCCCCHHEEEEEECCCCEEEECCCCEEEEECCCCEEEEEEECCEEECCHHHHHHHHHHHH
RMSAAYDNYHLFGQLKKSQEASVVAMASLAEHKDEDTGLHVLRVRDFTEAIANELLQNGH
HHHHHCCCCCEEHHHHCCCCCHHHHHHHHHHCCCCCCCEEEEEEHHHHHHHHHHHHHCCC
FTDELTQGFLSKVGWASILHDIGKIGIPDCILQKKGKLDSDERTIMETHARMGEDILFKA
CHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHCCCCCCCCHHHHHHHHHHCCHHHHHHH
SELIQGRNYFSIAADIAGGHHEYFDGNGYPRGLSGEQIPLSARIVAVVDVFDALMSERPY
HHHHCCCCEEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCC
KTAWPLNQAIDYILGRSSSQFDPLVVQAILALVTEKRLPPVLCEYYGLGKH
CCCCCHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHCCCCHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11123673 [H]