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The map label for this gene is purN [H]

Identifier: 126173963

GI number: 126173963

Start: 2018060

End: 2018704

Strand: Reverse

Name: purN [H]

Synonym: Sbal_1734

Alternate gene names: 126173963

Gene position: 2018704-2018060 (Counterclockwise)

Preceding gene: 126173964

Following gene: 126173962

Centisome position: 39.37

GC content: 48.37

Gene sequence:

>645_bases
ATGCCCCAGAGCTGTCGTGTAGTTGTATTAATTTCCGGAAATGGCAGTAACCTGCAAGCGATTATCGACGGTTGCGACGA
TAATCTGCAGGCCGAAGTTGTCGGAGTCATCAGTAATAAACCCGATGCCTATGGCCTAGTGCGCGCCCATCATGGCGAAA
TTGATACCAGCTGCGTGATAGCTCACCAAGGTGAATCTCGCTCTGAATACGATGCACGTTTAATGACAGTCATCGAACAG
TACCAACCCGATTTAATCGTGTTGGCAGGATTTATGCGGATCTTAACCGATGATTTCGTGAATCATTATCTAGGTCGCAT
GATCAATATCCATCCTTCACTGTTACCGAAATACACTGGCTTAAACACCCATCAACGTGCCATTGACGCTAACGACAGCG
AACATGGTGCCAGCGTGCATTTTGTCACGCCAGAGCTTGATGCAGGTCCGGTGATTTTACAGGCAAAAGTACCTGTTTAT
GAAGAAGATACCGCTGACATGCTCGCCGCCCGCGTGCATGAGCAAGAACATGCCATTTACCCACTCGTGGTGAAATGGTT
TAGTCAGCAACGTCTTAACATGCAAAACGGCCAAGCCTATCTTGATGGCAATCTTATCGGCCCGAGCGGTTACGCGCCTG
ATTAG

Upstream 100 bases:

>100_bases
ATGCAGCACTTGCATTACTGGCTGCAGAAGGCGAACAAGCTTGGCTGATCGGTGCTATCGCAGATCGTGAAGGCAATGAA
GAGCAAGTGGAGATCCTGTA

Downstream 100 bases:

>100_bases
TCCATCACTTCGTCAGTCAGACTTAGATCACAAGTAGAGACCTTCGGGTCTCTATTTTTTTAACTATTTCCCACAAAAGC
ATTGTTTTCTAGGGCAATGT

Product: phosphoribosylglycinamide formyltransferase

Products: NA

Alternate protein names: 5'-phosphoribosylglycinamide transformylase; GAR transformylase; GART [H]

Number of amino acids: Translated: 214; Mature: 213

Protein sequence:

>214_residues
MPQSCRVVVLISGNGSNLQAIIDGCDDNLQAEVVGVISNKPDAYGLVRAHHGEIDTSCVIAHQGESRSEYDARLMTVIEQ
YQPDLIVLAGFMRILTDDFVNHYLGRMINIHPSLLPKYTGLNTHQRAIDANDSEHGASVHFVTPELDAGPVILQAKVPVY
EEDTADMLAARVHEQEHAIYPLVVKWFSQQRLNMQNGQAYLDGNLIGPSGYAPD

Sequences:

>Translated_214_residues
MPQSCRVVVLISGNGSNLQAIIDGCDDNLQAEVVGVISNKPDAYGLVRAHHGEIDTSCVIAHQGESRSEYDARLMTVIEQ
YQPDLIVLAGFMRILTDDFVNHYLGRMINIHPSLLPKYTGLNTHQRAIDANDSEHGASVHFVTPELDAGPVILQAKVPVY
EEDTADMLAARVHEQEHAIYPLVVKWFSQQRLNMQNGQAYLDGNLIGPSGYAPD
>Mature_213_residues
PQSCRVVVLISGNGSNLQAIIDGCDDNLQAEVVGVISNKPDAYGLVRAHHGEIDTSCVIAHQGESRSEYDARLMTVIEQY
QPDLIVLAGFMRILTDDFVNHYLGRMINIHPSLLPKYTGLNTHQRAIDANDSEHGASVHFVTPELDAGPVILQAKVPVYE
EDTADMLAARVHEQEHAIYPLVVKWFSQQRLNMQNGQAYLDGNLIGPSGYAPD

Specific function: De novo purine biosynthesis; third step. [C]

COG id: COG0299

COG function: function code F; Folate-dependent phosphoribosylglycinamide formyltransferase PurN

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GART family [H]

Homologues:

Organism=Homo sapiens, GI4503915, Length=198, Percent_Identity=41.4141414141414, Blast_Score=155, Evalue=2e-38,
Organism=Homo sapiens, GI209869995, Length=198, Percent_Identity=41.4141414141414, Blast_Score=155, Evalue=2e-38,
Organism=Homo sapiens, GI209869993, Length=198, Percent_Identity=41.4141414141414, Blast_Score=155, Evalue=2e-38,
Organism=Escherichia coli, GI1788846, Length=209, Percent_Identity=59.3301435406699, Blast_Score=252, Evalue=1e-68,
Organism=Escherichia coli, GI1787483, Length=187, Percent_Identity=30.4812834224599, Blast_Score=93, Evalue=1e-20,
Organism=Caenorhabditis elegans, GI17567511, Length=177, Percent_Identity=36.7231638418079, Blast_Score=109, Evalue=8e-25,
Organism=Saccharomyces cerevisiae, GI6320616, Length=197, Percent_Identity=32.994923857868, Blast_Score=89, Evalue=4e-19,
Organism=Drosophila melanogaster, GI24582400, Length=180, Percent_Identity=49.4444444444444, Blast_Score=164, Evalue=3e-41,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002376
- InterPro:   IPR001555
- InterPro:   IPR004607 [H]

Pfam domain/function: PF00551 Formyl_trans_N [H]

EC number: =2.1.2.2 [H]

Molecular weight: Translated: 23575; Mature: 23444

Theoretical pI: Translated: 4.74; Mature: 4.74

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPQSCRVVVLISGNGSNLQAIIDGCDDNLQAEVVGVISNKPDAYGLVRAHHGEIDTSCVI
CCCCCEEEEEEECCCCCEEEEEECCCCCCEEEEEEEECCCCCCEEEEEEECCCCCCEEEE
AHQGESRSEYDARLMTVIEQYQPDLIVLAGFMRILTDDFVNHYLGRMINIHPSLLPKYTG
EECCCCCCHHHHHHHHHHHHHCCCEEHHHHHHHHHHHHHHHHHHHHEEECCHHHCCCCCC
LNTHQRAIDANDSEHGASVHFVTPELDAGPVILQAKVPVYEEDTADMLAARVHEQEHAIY
CCCHHHCCCCCCCCCCCEEEEECCCCCCCCEEEEEECCCCCCCHHHHHHHHHHHHCCCHH
PLVVKWFSQQRLNMQNGQAYLDGNLIGPSGYAPD
HHHHHHHHHHHCCCCCCCEEECCEEECCCCCCCC
>Mature Secondary Structure 
PQSCRVVVLISGNGSNLQAIIDGCDDNLQAEVVGVISNKPDAYGLVRAHHGEIDTSCVI
CCCCEEEEEEECCCCCEEEEEECCCCCCEEEEEEEECCCCCCEEEEEEECCCCCCEEEE
AHQGESRSEYDARLMTVIEQYQPDLIVLAGFMRILTDDFVNHYLGRMINIHPSLLPKYTG
EECCCCCCHHHHHHHHHHHHHCCCEEHHHHHHHHHHHHHHHHHHHHEEECCHHHCCCCCC
LNTHQRAIDANDSEHGASVHFVTPELDAGPVILQAKVPVYEEDTADMLAARVHEQEHAIY
CCCHHHCCCCCCCCCCCEEEEECCCCCCCCEEEEEECCCCCCCHHHHHHHHHHHHCCCHH
PLVVKWFSQQRLNMQNGQAYLDGNLIGPSGYAPD
HHHHHHHHHHHCCCCCCCEEECCEEECCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 3301838; 9205837; 9278503; 10954745; 2204419; 1522592; 1631098; 9698564; 10606510 [H]