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The map label for this gene is proB

Identifier: 124265707

GI number: 124265707

Start: 552323

End: 553441

Strand: Direct

Name: proB

Synonym: Mpe_A0514

Alternate gene names: 124265707

Gene position: 552323-553441 (Clockwise)

Preceding gene: 124265706

Following gene: 124265708

Centisome position: 13.66

GC content: 66.93

Gene sequence:

>1119_bases
ATGTCCGAAGTGCTGCGTGGAGCGAGGCGCATCGTCGTCAAGGTCGGATCCAGCCTCGTGACCAACGAGGGGCGCGGTGT
CGATGCCGCCGCGATCGGCAACTGGTGCCGCCAGCTCGCGTCGCTGGCGGGTCAGGGGCGCGAGGTGCTGATGGTGTCCA
GCGGCGCGATCGCCGAAGGCATGAAGCGCTTGGGCTGGAACGCGCGACCGAAGGAGATTCATGAGTTGCAGGCCGCTGCC
GCCGTCGGACAGATGGGGCTGGCGCAGATGTACGAGAGCAAGCTTTCGGAGCATGGCATCGGCAGCGCGCAGGTGCTGTT
GACGCACGCCGACCTCGCGGACCGCGAGCGCTACCTGAACGCACGCTCGACCCTGCTCACCCTGTTGTCACTGAAGGTGA
TCCCGGTCATCAACGAGAACGACACGGTCGTCAACGACGAGATCAAGTTCGGCGACAACGACACGCTGGGCGCACTGGTC
GCCAACCTCGTCGACGCTGATGCGCTGGTGATCCTGACCGATCAGCCTGGCCTGTATTCCGCCGATCCGCGCAAGGACCC
GCAGGCCCGGTTCATCGGTGAGGCTGTCGCCGGAACGCCGGAACTCGAGCGGATGGCAGGTGGTGCGGGCTCCAGCCTCG
GGCGTGGCGGCATGATCACCAAGGTGCTGGCCGCCAAGCGTGCCTCCAGCAGCGGGGCCTCGACCGTTATCGCCTGGGGT
CGTGAACCCGACGTGCTGCTGCGACTCGCTGACGGTGAGGCGATCGGCACGCTGCTGGTCGCTCGCACTGCCAAGCTCGC
GGCGCGCAAGCAGTGGATGGCCGACCATCTGCAGATGCGCGGCACGGTCGTCATCGACGACGGCGCCGTCGCCAAGCTGC
GCGACGAAGGCAAGAGTCTGCTGCCGATCGGCGTGGTCGAGGTGCAGGGCGAGTTCGTGCGAGGTGACGTGATCGCGGTG
CGGAGCCAGGTCGGTATCGAAATCGCGCGGGGCCTGGCCAACTACGCGAGCAGCGAGGCGCGGCTGATCGCGCGCAAGCC
GTCGTCGCAGATCGAGAGTCTGCTGGGCTACAGCAACGAGCCCGAGATGATCCACCGCACGAATCTCGTGCTGGCCTGA

Upstream 100 bases:

>100_bases
GGCCGACGAGAGCGATGACGAGCCCCGCTTTGCACCGCAGGCGGATGACCCGCGCTTCCGTTGAAGCGCGCCTGTTTCGG
ACCGAGAGAGTAGAGGCCGC

Downstream 100 bases:

>100_bases
CGAGAACTCTTGCGTCTGCGCTCTGGCTGTCCCCGAATCCCCCGCATCCGGTGCTCCCGACGCGGTGCGGAGCACCCCGC
GTGACGACGGAGCGCGAACC

Product: gamma-glutamyl kinase

Products: NA

Alternate protein names: Gamma-glutamyl kinase; GK

Number of amino acids: Translated: 372; Mature: 371

Protein sequence:

>372_residues
MSEVLRGARRIVVKVGSSLVTNEGRGVDAAAIGNWCRQLASLAGQGREVLMVSSGAIAEGMKRLGWNARPKEIHELQAAA
AVGQMGLAQMYESKLSEHGIGSAQVLLTHADLADRERYLNARSTLLTLLSLKVIPVINENDTVVNDEIKFGDNDTLGALV
ANLVDADALVILTDQPGLYSADPRKDPQARFIGEAVAGTPELERMAGGAGSSLGRGGMITKVLAAKRASSSGASTVIAWG
REPDVLLRLADGEAIGTLLVARTAKLAARKQWMADHLQMRGTVVIDDGAVAKLRDEGKSLLPIGVVEVQGEFVRGDVIAV
RSQVGIEIARGLANYASSEARLIARKPSSQIESLLGYSNEPEMIHRTNLVLA

Sequences:

>Translated_372_residues
MSEVLRGARRIVVKVGSSLVTNEGRGVDAAAIGNWCRQLASLAGQGREVLMVSSGAIAEGMKRLGWNARPKEIHELQAAA
AVGQMGLAQMYESKLSEHGIGSAQVLLTHADLADRERYLNARSTLLTLLSLKVIPVINENDTVVNDEIKFGDNDTLGALV
ANLVDADALVILTDQPGLYSADPRKDPQARFIGEAVAGTPELERMAGGAGSSLGRGGMITKVLAAKRASSSGASTVIAWG
REPDVLLRLADGEAIGTLLVARTAKLAARKQWMADHLQMRGTVVIDDGAVAKLRDEGKSLLPIGVVEVQGEFVRGDVIAV
RSQVGIEIARGLANYASSEARLIARKPSSQIESLLGYSNEPEMIHRTNLVLA
>Mature_371_residues
SEVLRGARRIVVKVGSSLVTNEGRGVDAAAIGNWCRQLASLAGQGREVLMVSSGAIAEGMKRLGWNARPKEIHELQAAAA
VGQMGLAQMYESKLSEHGIGSAQVLLTHADLADRERYLNARSTLLTLLSLKVIPVINENDTVVNDEIKFGDNDTLGALVA
NLVDADALVILTDQPGLYSADPRKDPQARFIGEAVAGTPELERMAGGAGSSLGRGGMITKVLAAKRASSSGASTVIAWGR
EPDVLLRLADGEAIGTLLVARTAKLAARKQWMADHLQMRGTVVIDDGAVAKLRDEGKSLLPIGVVEVQGEFVRGDVIAVR
SQVGIEIARGLANYASSEARLIARKPSSQIESLLGYSNEPEMIHRTNLVLA

Specific function: Catalyzes the transfer of a phosphate group to glutamate to form glutamate 5-phosphate which rapidly cyclizes to 5- oxoproline

COG id: COG0263

COG function: function code E; Glutamate 5-kinase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PUA domain

Homologues:

Organism=Homo sapiens, GI62912457, Length=288, Percent_Identity=33.6805555555556, Blast_Score=134, Evalue=1e-31,
Organism=Homo sapiens, GI21361368, Length=290, Percent_Identity=33.1034482758621, Blast_Score=134, Evalue=2e-31,
Organism=Escherichia coli, GI1786437, Length=363, Percent_Identity=43.5261707988981, Blast_Score=276, Evalue=1e-75,
Organism=Caenorhabditis elegans, GI17569969, Length=272, Percent_Identity=34.5588235294118, Blast_Score=135, Evalue=4e-32,
Organism=Caenorhabditis elegans, GI17569967, Length=278, Percent_Identity=33.8129496402878, Blast_Score=134, Evalue=5e-32,
Organism=Saccharomyces cerevisiae, GI6320506, Length=420, Percent_Identity=34.7619047619048, Blast_Score=198, Evalue=1e-51,
Organism=Saccharomyces cerevisiae, GI6321822, Length=419, Percent_Identity=33.1742243436754, Blast_Score=190, Evalue=3e-49,
Organism=Drosophila melanogaster, GI21357643, Length=291, Percent_Identity=33.6769759450172, Blast_Score=138, Evalue=7e-33,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): PROB_METPP (A2SD37)

Other databases:

- EMBL:   CP000555
- RefSeq:   YP_001019711.1
- ProteinModelPortal:   A2SD37
- STRING:   A2SD37
- GeneID:   4787090
- GenomeReviews:   CP000555_GR
- KEGG:   mpt:Mpe_A0514
- NMPDR:   fig|279263.3.peg.2758
- eggNOG:   COG0263
- HOGENOM:   HBG507643
- OMA:   TFGDNDM
- PhylomeDB:   A2SD37
- ProtClustDB:   PRK05429
- BioCyc:   MPET420662:MPE_A0514-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00456
- InterPro:   IPR001048
- InterPro:   IPR001057
- InterPro:   IPR011529
- InterPro:   IPR005715
- InterPro:   IPR019797
- InterPro:   IPR002478
- InterPro:   IPR015947
- Gene3D:   G3DSA:3.40.1160.10
- PIRSF:   PIRSF000729
- PRINTS:   PR00474
- SMART:   SM00359
- TIGRFAMs:   TIGR01027

Pfam domain/function: PF00696 AA_kinase; PF01472 PUA; SSF53633 Aa_kinase; SSF88697 PUA-like

EC number: =2.7.2.11

Molecular weight: Translated: 39425; Mature: 39294

Theoretical pI: Translated: 7.09; Mature: 7.09

Prosite motif: PS00902 GLUTAMATE_5_KINASE; PS50890 PUA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSEVLRGARRIVVKVGSSLVTNEGRGVDAAAIGNWCRQLASLAGQGREVLMVSSGAIAEG
CCHHHHHHHHHHHHHCCHHCCCCCCCCCHHHHHHHHHHHHHHHCCCCEEEEEECCHHHHH
MKRLGWNARPKEIHELQAAAAVGQMGLAQMYESKLSEHGIGSAQVLLTHADLADRERYLN
HHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCHHHHHHHHH
ARSTLLTLLSLKVIPVINENDTVVNDEIKFGDNDTLGALVANLVDADALVILTDQPGLYS
HHHHHHHHHHHHEEEEECCCCCEECCEEECCCCCHHHHHHHHHHCCCEEEEEECCCCCCC
ADPRKDPQARFIGEAVAGTPELERMAGGAGSSLGRGGMITKVLAAKRASSSGASTVIAWG
CCCCCCCHHHHHHHHHCCCCHHHHHCCCCCCCCCCCCHHHHHHHHHHCCCCCCCEEEEEC
REPDVLLRLADGEAIGTLLVARTAKLAARKQWMADHLQMRGTVVIDDGAVAKLRDEGKSL
CCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEECCCCEEHHHHCCCCC
LPIGVVEVQGEFVRGDVIAVRSQVGIEIARGLANYASSEARLIARKPSSQIESLLGYSNE
CEEEEEEECCCEECCCEEEHHHHHHHHHHHHHHHHCCCCCEEEECCCHHHHHHHHCCCCC
PEMIHRTNLVLA
CCCEECCCEEEC
>Mature Secondary Structure 
SEVLRGARRIVVKVGSSLVTNEGRGVDAAAIGNWCRQLASLAGQGREVLMVSSGAIAEG
CHHHHHHHHHHHHHCCHHCCCCCCCCCHHHHHHHHHHHHHHHCCCCEEEEEECCHHHHH
MKRLGWNARPKEIHELQAAAAVGQMGLAQMYESKLSEHGIGSAQVLLTHADLADRERYLN
HHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCHHHHHHHHH
ARSTLLTLLSLKVIPVINENDTVVNDEIKFGDNDTLGALVANLVDADALVILTDQPGLYS
HHHHHHHHHHHHEEEEECCCCCEECCEEECCCCCHHHHHHHHHHCCCEEEEEECCCCCCC
ADPRKDPQARFIGEAVAGTPELERMAGGAGSSLGRGGMITKVLAAKRASSSGASTVIAWG
CCCCCCCHHHHHHHHHCCCCHHHHHCCCCCCCCCCCCHHHHHHHHHHCCCCCCCEEEEEC
REPDVLLRLADGEAIGTLLVARTAKLAARKQWMADHLQMRGTVVIDDGAVAKLRDEGKSL
CCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEECCCCEEHHHHCCCCC
LPIGVVEVQGEFVRGDVIAVRSQVGIEIARGLANYASSEARLIARKPSSQIESLLGYSNE
CEEEEEEECCCEECCCEEEHHHHHHHHHHHHHHHHCCCCCEEEECCCHHHHHHHHCCCCC
PEMIHRTNLVLA
CCCEECCCEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA