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The map label for this gene is prpE [H]

Identifier: 124265588

GI number: 124265588

Start: 427626

End: 429521

Strand: Direct

Name: prpE [H]

Synonym: Mpe_A0395

Alternate gene names: 124265588

Gene position: 427626-429521 (Clockwise)

Preceding gene: 124265587

Following gene: 124265590

Centisome position: 10.57

GC content: 68.72

Gene sequence:

>1896_bases
ATGACCGACTACGCCACCTTCTACCGCCGCTCGATCGAAGACCCGAAGGGTTTCTGGGCCGAGCAGGCCGAGCTGATCGA
CTGGCACCGCCCGTTCGACGAAGTGCTCGACGACAGCCGGCCGCCCTTCACCCGCTGGTTCGTCGGCGGCCAGACCAACC
TCTGCCACAACGCCGTCGACCGCCACGCCGCCCGGCGCCCCGACGACAACGCCCTCATCTGGGTCTCCACCGAAGTCGGT
CAGGAACGCACCTACAGCTACGCCCAACTCCAGCGCGAAGTCGAACGCATGGCCGCCGGCCTCGTCGAACTCGGCGTCGG
CCAGGGCGACCGCGTCCTCATCTACATGCCCATGATCCCCGAGGCCGCCTTCGCCATGCTCGCCTGCGTGCGCATCGGCG
CCATCCACTCCGTCGTCTTCGGCGGCTTCGCCAGCGTCAGCCTCGCCAGCCGCATCGACGACGCCCAACCCAAACTCATC
GTCAGCGCCGACGCCGGCAGCCGCGCCGGCAAAGTCGTCCCCTACAAACCCCTCCTCGACGAAGCCATCCGCCTGGCCAA
GAACAAACCCCGGCACGTCCTCATGGTCGACCGCGGCCTCGCCCCCAGCCAACCCGTCGAAGGCCGCGACATCGACTACG
CCACCCTCAGGGCGCGCCACCTCGACAGCCGCGTCCCCTGCACCTGGGTCGACGCCACCCACCCCAGCTACACCCTCTAC
ACCAGCGGCACCACCGGCACCCCCAAGGGCGTACAGCGCGACACCGGCGGCTACGCCGTCGCGCTGGCCGCCAGCATGAA
ACACATCTACCTCGGCAACGCCGGCGAAACCTACTTCTCCACCAGCGACATCGGCTGGGTCGTCGGCCACAGCTACATCG
TCTACGGCCCGCTCCTCGCCGGCATGGCCACCATCATGTACGAAGGCCTGCCCATCCGCCCCGACGCCGCCATCTGGTGG
AGCCTGGTCGAGAAATACAAAGTCACCGCCATGTTCAGCGCCCCCACCGCCGTGCGCGTGCTCAAGAAACAAGACCCCGC
GGCCCTCAAGAAACACGACCTCAGCAGCCTGCGCGCCCTGTTCCTGGCCGGAGAGCCGCTCGACGAACCCACCGCCCAGT
GGATCGCCGACGCCCTGGGCGTGCCCATCGTCGACAACTACTGGCAGACCGAATCCGGCTGGCCCATCCTCAGCATCGCC
AACGGCGTGCAGCGCCAGCCCAGCAAGTTCGGCAGCCCCGGCATCCCCACCTACGGCTACAAGGTCAAGCTCGTCGACGA
CGCCAGCGGCGAAGAACTGCACGGCGCCCACCAGAAAGGCGTGCTCGTGGTCGAAGGCCCGCTGCCGCCCGGCTTCATGC
AGACCGTCTGGCGCGACGACGACCGCTTCGTCAACACCTACTGGAAAAGCGTGCCCGGCCAGAGCGTCTACAGCAGCTTC
GACTGGGGCATCCGGGACGAAGACGGCTACTACTACATCCTCGGGCGCACCGACGACGTCATCAACGTCGCCGGGCACCG
GCTGGGCACCCGCGAGATCGAGGAAAGCGTCTCCAGCCACGCCAACGTGGCCGAAGTGGCGGTGGTCGGGGTGGCCGATC
AGCTCAAGGGCCAGGTGGCCGTGGCCTTCGTGGTGCCCAAGGACGCGCGGGGGCTGGAGGAGCCGCAGGCGCGGCTGAAG
CTCGAGGGCGACATCATGAAGACGGTGGACCAGCAGCTCGGGGCGGTGGCGAGGCCGGCGCGGGTGCACTTCGTGACGCT
GCTGCCCAAGACCCGCTCGGGCAAGCTGCTGCGCCGTGCGCTGCAGGCGGTGGCCGAGGGCCGCGACCCGGGCGACCTGA
CCACCATCGAGGACCCCACGGCCTTGCAGGCGATCCGGGAGTTGCTCGGGCGGTGA

Upstream 100 bases:

>100_bases
TCGGCGGCTACTGGGCCACGCAGATCCTGCCGCTGATGGACGCCGCGTCCTACCCGGGCTTCGTGGAGTTCGAGTCCACC
GTCTACCAGCACCGCAGCGC

Downstream 100 bases:

>100_bases
GGCAGGGGGCGGTGGCCGCGCGGCGGCCACCGTTCAGTGGATGCCGTCGCGCGCGATCGGCCGCTCGTCGTGGCCCGCCT
GGGTGAACTCGCTCTGGTCG

Product: propionyl-CoA synthetase

Products: NA

Alternate protein names: Propionyl-CoA synthetase [H]

Number of amino acids: Translated: 631; Mature: 630

Protein sequence:

>631_residues
MTDYATFYRRSIEDPKGFWAEQAELIDWHRPFDEVLDDSRPPFTRWFVGGQTNLCHNAVDRHAARRPDDNALIWVSTEVG
QERTYSYAQLQREVERMAAGLVELGVGQGDRVLIYMPMIPEAAFAMLACVRIGAIHSVVFGGFASVSLASRIDDAQPKLI
VSADAGSRAGKVVPYKPLLDEAIRLAKNKPRHVLMVDRGLAPSQPVEGRDIDYATLRARHLDSRVPCTWVDATHPSYTLY
TSGTTGTPKGVQRDTGGYAVALAASMKHIYLGNAGETYFSTSDIGWVVGHSYIVYGPLLAGMATIMYEGLPIRPDAAIWW
SLVEKYKVTAMFSAPTAVRVLKKQDPAALKKHDLSSLRALFLAGEPLDEPTAQWIADALGVPIVDNYWQTESGWPILSIA
NGVQRQPSKFGSPGIPTYGYKVKLVDDASGEELHGAHQKGVLVVEGPLPPGFMQTVWRDDDRFVNTYWKSVPGQSVYSSF
DWGIRDEDGYYYILGRTDDVINVAGHRLGTREIEESVSSHANVAEVAVVGVADQLKGQVAVAFVVPKDARGLEEPQARLK
LEGDIMKTVDQQLGAVARPARVHFVTLLPKTRSGKLLRRALQAVAEGRDPGDLTTIEDPTALQAIRELLGR

Sequences:

>Translated_631_residues
MTDYATFYRRSIEDPKGFWAEQAELIDWHRPFDEVLDDSRPPFTRWFVGGQTNLCHNAVDRHAARRPDDNALIWVSTEVG
QERTYSYAQLQREVERMAAGLVELGVGQGDRVLIYMPMIPEAAFAMLACVRIGAIHSVVFGGFASVSLASRIDDAQPKLI
VSADAGSRAGKVVPYKPLLDEAIRLAKNKPRHVLMVDRGLAPSQPVEGRDIDYATLRARHLDSRVPCTWVDATHPSYTLY
TSGTTGTPKGVQRDTGGYAVALAASMKHIYLGNAGETYFSTSDIGWVVGHSYIVYGPLLAGMATIMYEGLPIRPDAAIWW
SLVEKYKVTAMFSAPTAVRVLKKQDPAALKKHDLSSLRALFLAGEPLDEPTAQWIADALGVPIVDNYWQTESGWPILSIA
NGVQRQPSKFGSPGIPTYGYKVKLVDDASGEELHGAHQKGVLVVEGPLPPGFMQTVWRDDDRFVNTYWKSVPGQSVYSSF
DWGIRDEDGYYYILGRTDDVINVAGHRLGTREIEESVSSHANVAEVAVVGVADQLKGQVAVAFVVPKDARGLEEPQARLK
LEGDIMKTVDQQLGAVARPARVHFVTLLPKTRSGKLLRRALQAVAEGRDPGDLTTIEDPTALQAIRELLGR
>Mature_630_residues
TDYATFYRRSIEDPKGFWAEQAELIDWHRPFDEVLDDSRPPFTRWFVGGQTNLCHNAVDRHAARRPDDNALIWVSTEVGQ
ERTYSYAQLQREVERMAAGLVELGVGQGDRVLIYMPMIPEAAFAMLACVRIGAIHSVVFGGFASVSLASRIDDAQPKLIV
SADAGSRAGKVVPYKPLLDEAIRLAKNKPRHVLMVDRGLAPSQPVEGRDIDYATLRARHLDSRVPCTWVDATHPSYTLYT
SGTTGTPKGVQRDTGGYAVALAASMKHIYLGNAGETYFSTSDIGWVVGHSYIVYGPLLAGMATIMYEGLPIRPDAAIWWS
LVEKYKVTAMFSAPTAVRVLKKQDPAALKKHDLSSLRALFLAGEPLDEPTAQWIADALGVPIVDNYWQTESGWPILSIAN
GVQRQPSKFGSPGIPTYGYKVKLVDDASGEELHGAHQKGVLVVEGPLPPGFMQTVWRDDDRFVNTYWKSVPGQSVYSSFD
WGIRDEDGYYYILGRTDDVINVAGHRLGTREIEESVSSHANVAEVAVVGVADQLKGQVAVAFVVPKDARGLEEPQARLKL
EGDIMKTVDQQLGAVARPARVHFVTLLPKTRSGKLLRRALQAVAEGRDPGDLTTIEDPTALQAIRELLGR

Specific function: Catalyzes the synthesis of propionyl-CoA from propionate and CoA. Also converts acetate to acetyl-CoA but with a lower specific activity [H]

COG id: COG0365

COG function: function code I; Acyl-coenzyme A synthetases/AMP-(fatty) acid ligases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATP-dependent AMP-binding enzyme family [H]

Homologues:

Organism=Homo sapiens, GI13375727, Length=635, Percent_Identity=43.4645669291339, Blast_Score=513, Evalue=1e-145,
Organism=Homo sapiens, GI8923896, Length=659, Percent_Identity=37.1775417298938, Blast_Score=414, Evalue=1e-115,
Organism=Homo sapiens, GI253970489, Length=675, Percent_Identity=36.2962962962963, Blast_Score=409, Evalue=1e-114,
Organism=Homo sapiens, GI28416953, Length=636, Percent_Identity=36.7924528301887, Blast_Score=397, Evalue=1e-110,
Organism=Homo sapiens, GI38505220, Length=565, Percent_Identity=26.9026548672566, Blast_Score=179, Evalue=6e-45,
Organism=Homo sapiens, GI42544132, Length=546, Percent_Identity=26.3736263736264, Blast_Score=166, Evalue=5e-41,
Organism=Homo sapiens, GI122937307, Length=572, Percent_Identity=26.3986013986014, Blast_Score=160, Evalue=3e-39,
Organism=Homo sapiens, GI31982927, Length=652, Percent_Identity=24.2331288343558, Blast_Score=156, Evalue=6e-38,
Organism=Homo sapiens, GI58082049, Length=557, Percent_Identity=25.8527827648115, Blast_Score=152, Evalue=1e-36,
Organism=Homo sapiens, GI115511026, Length=560, Percent_Identity=25.7142857142857, Blast_Score=150, Evalue=3e-36,
Organism=Homo sapiens, GI157311624, Length=559, Percent_Identity=25.5813953488372, Blast_Score=150, Evalue=5e-36,
Organism=Homo sapiens, GI157311622, Length=559, Percent_Identity=25.5813953488372, Blast_Score=150, Evalue=5e-36,
Organism=Homo sapiens, GI42544134, Length=372, Percent_Identity=23.6559139784946, Blast_Score=96, Evalue=1e-19,
Organism=Homo sapiens, GI195947378, Length=488, Percent_Identity=21.3114754098361, Blast_Score=94, Evalue=4e-19,
Organism=Homo sapiens, GI187761345, Length=399, Percent_Identity=23.8095238095238, Blast_Score=77, Evalue=5e-14,
Organism=Homo sapiens, GI187761343, Length=399, Percent_Identity=23.8095238095238, Blast_Score=77, Evalue=5e-14,
Organism=Escherichia coli, GI1786529, Length=626, Percent_Identity=63.7380191693291, Blast_Score=822, Evalue=0.0,
Organism=Escherichia coli, GI1790505, Length=633, Percent_Identity=39.652448657188, Blast_Score=452, Evalue=1e-128,
Organism=Escherichia coli, GI221142682, Length=544, Percent_Identity=21.3235294117647, Blast_Score=120, Evalue=2e-28,
Organism=Escherichia coli, GI145693145, Length=545, Percent_Identity=23.4862385321101, Blast_Score=107, Evalue=2e-24,
Organism=Escherichia coli, GI1788107, Length=587, Percent_Identity=24.3611584327087, Blast_Score=96, Evalue=7e-21,
Organism=Escherichia coli, GI1786810, Length=527, Percent_Identity=22.011385199241, Blast_Score=81, Evalue=2e-16,
Organism=Escherichia coli, GI1786801, Length=529, Percent_Identity=22.4952741020794, Blast_Score=75, Evalue=1e-14,
Organism=Caenorhabditis elegans, GI71982997, Length=650, Percent_Identity=36, Blast_Score=364, Evalue=1e-101,
Organism=Caenorhabditis elegans, GI71983001, Length=650, Percent_Identity=36, Blast_Score=364, Evalue=1e-101,
Organism=Caenorhabditis elegans, GI17531443, Length=558, Percent_Identity=26.8817204301075, Blast_Score=134, Evalue=1e-31,
Organism=Caenorhabditis elegans, GI17569507, Length=655, Percent_Identity=22.2900763358779, Blast_Score=121, Evalue=1e-27,
Organism=Caenorhabditis elegans, GI17557194, Length=599, Percent_Identity=24.2070116861436, Blast_Score=103, Evalue=4e-22,
Organism=Caenorhabditis elegans, GI17538037, Length=577, Percent_Identity=23.2235701906412, Blast_Score=99, Evalue=9e-21,
Organism=Caenorhabditis elegans, GI71996755, Length=536, Percent_Identity=21.455223880597, Blast_Score=98, Evalue=1e-20,
Organism=Caenorhabditis elegans, GI71985884, Length=581, Percent_Identity=23.2358003442341, Blast_Score=94, Evalue=2e-19,
Organism=Caenorhabditis elegans, GI17558820, Length=530, Percent_Identity=22.4528301886792, Blast_Score=94, Evalue=3e-19,
Organism=Caenorhabditis elegans, GI17560140, Length=532, Percent_Identity=21.8045112781955, Blast_Score=89, Evalue=5e-18,
Organism=Caenorhabditis elegans, GI17559526, Length=570, Percent_Identity=22.9824561403509, Blast_Score=80, Evalue=3e-15,
Organism=Saccharomyces cerevisiae, GI6319264, Length=636, Percent_Identity=40.8805031446541, Blast_Score=484, Evalue=1e-137,
Organism=Saccharomyces cerevisiae, GI6323182, Length=640, Percent_Identity=39.21875, Blast_Score=441, Evalue=1e-124,
Organism=Saccharomyces cerevisiae, GI6319699, Length=370, Percent_Identity=22.972972972973, Blast_Score=84, Evalue=8e-17,
Organism=Drosophila melanogaster, GI21355225, Length=641, Percent_Identity=40.8736349453978, Blast_Score=483, Evalue=1e-136,
Organism=Drosophila melanogaster, GI24667955, Length=651, Percent_Identity=36.8663594470046, Blast_Score=397, Evalue=1e-111,
Organism=Drosophila melanogaster, GI62472339, Length=561, Percent_Identity=38.1461675579323, Blast_Score=361, Evalue=1e-100,
Organism=Drosophila melanogaster, GI24667959, Length=528, Percent_Identity=37.6893939393939, Blast_Score=336, Evalue=3e-92,
Organism=Drosophila melanogaster, GI21355181, Length=534, Percent_Identity=26.0299625468165, Blast_Score=118, Evalue=2e-26,
Organism=Drosophila melanogaster, GI24648260, Length=387, Percent_Identity=27.1317829457364, Blast_Score=86, Evalue=7e-17,
Organism=Drosophila melanogaster, GI24648257, Length=372, Percent_Identity=27.4193548387097, Blast_Score=80, Evalue=3e-15,
Organism=Drosophila melanogaster, GI21356947, Length=371, Percent_Identity=25.0673854447439, Blast_Score=80, Evalue=5e-15,
Organism=Drosophila melanogaster, GI21358303, Length=497, Percent_Identity=22.1327967806841, Blast_Score=77, Evalue=3e-14,
Organism=Drosophila melanogaster, GI20130357, Length=387, Percent_Identity=25.3229974160207, Blast_Score=67, Evalue=4e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020845
- InterPro:   IPR000873
- InterPro:   IPR012694 [H]

Pfam domain/function: PF00501 AMP-binding [H]

EC number: =6.2.1.17 [H]

Molecular weight: Translated: 69405; Mature: 69273

Theoretical pI: Translated: 6.47; Mature: 6.47

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTDYATFYRRSIEDPKGFWAEQAELIDWHRPFDEVLDDSRPPFTRWFVGGQTNLCHNAVD
CCCHHHHHHHHCCCCCCCHHHHHHHHHHCCCHHHHHCCCCCCCEEEEECCCCHHHHHHHH
RHAARRPDDNALIWVSTEVGQERTYSYAQLQREVERMAAGLVELGVGQGDRVLIYMPMIP
HHHHCCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCC
EAAFAMLACVRIGAIHSVVFGGFASVSLASRIDDAQPKLIVSADAGSRAGKVVPYKPLLD
HHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHCCCCCCCEEEEECCCCCCCCCCCCHHHHH
EAIRLAKNKPRHVLMVDRGLAPSQPVEGRDIDYATLRARHLDSRVPCTWVDATHPSYTLY
HHHHHHCCCCCEEEEEECCCCCCCCCCCCCCCHHHHHHHHCCCCCCEEEEECCCCCEEEE
TSGTTGTPKGVQRDTGGYAVALAASMKHIYLGNAGETYFSTSDIGWVVGHSYIVYGPLLA
ECCCCCCCCCCCCCCCCEEEEEEECCCEEEECCCCCCEEECCCCCHHHCCCHHHHHHHHH
GMATIMYEGLPIRPDAAIWWSLVEKYKVTAMFSAPTAVRVLKKQDPAALKKHDLSSLRAL
HHHHHHHCCCCCCCCHHHHHHHHHHHEEEEEECCCHHHHHHHCCCCHHHHHHHHHHHHHH
FLAGEPLDEPTAQWIADALGVPIVDNYWQTESGWPILSIANGVQRQPSKFGSPGIPTYGY
HHCCCCCCCHHHHHHHHHCCCCEECCCCCCCCCCEEEEECCCCCCCCHHCCCCCCCCCCE
KVKLVDDASGEELHGAHQKGVLVVEGPLPPGFMQTVWRDDDRFVNTYWKSVPGQSVYSSF
EEEEEECCCCCHHCCCCCCCEEEEECCCCCHHHHHHHCCCCHHHHHHHHCCCCHHHHHHC
DWGIRDEDGYYYILGRTDDVINVAGHRLGTREIEESVSSHANVAEVAVVGVADQLKGQVA
CCCCCCCCCEEEEEECCCCHHHHHHCCCCHHHHHHHHHCCCCEEHEEEEECHHHCCCCEE
VAFVVPKDARGLEEPQARLKLEGDIMKTVDQQLGAVARPARVHFVTLLPKTRSGKLLRRA
EEEEECCCCCCCCCCHHHEEECCHHHHHHHHHHHHHCCCCEEEEEEECCCCCCCHHHHHH
LQAVAEGRDPGDLTTIEDPTALQAIRELLGR
HHHHHCCCCCCCCCCCCCCHHHHHHHHHHCC
>Mature Secondary Structure 
TDYATFYRRSIEDPKGFWAEQAELIDWHRPFDEVLDDSRPPFTRWFVGGQTNLCHNAVD
CCHHHHHHHHCCCCCCCHHHHHHHHHHCCCHHHHHCCCCCCCEEEEECCCCHHHHHHHH
RHAARRPDDNALIWVSTEVGQERTYSYAQLQREVERMAAGLVELGVGQGDRVLIYMPMIP
HHHHCCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCC
EAAFAMLACVRIGAIHSVVFGGFASVSLASRIDDAQPKLIVSADAGSRAGKVVPYKPLLD
HHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHCCCCCCCEEEEECCCCCCCCCCCCHHHHH
EAIRLAKNKPRHVLMVDRGLAPSQPVEGRDIDYATLRARHLDSRVPCTWVDATHPSYTLY
HHHHHHCCCCCEEEEEECCCCCCCCCCCCCCCHHHHHHHHCCCCCCEEEEECCCCCEEEE
TSGTTGTPKGVQRDTGGYAVALAASMKHIYLGNAGETYFSTSDIGWVVGHSYIVYGPLLA
ECCCCCCCCCCCCCCCCEEEEEEECCCEEEECCCCCCEEECCCCCHHHCCCHHHHHHHHH
GMATIMYEGLPIRPDAAIWWSLVEKYKVTAMFSAPTAVRVLKKQDPAALKKHDLSSLRAL
HHHHHHHCCCCCCCCHHHHHHHHHHHEEEEEECCCHHHHHHHCCCCHHHHHHHHHHHHHH
FLAGEPLDEPTAQWIADALGVPIVDNYWQTESGWPILSIANGVQRQPSKFGSPGIPTYGY
HHCCCCCCCHHHHHHHHHCCCCEECCCCCCCCCCEEEEECCCCCCCCHHCCCCCCCCCCE
KVKLVDDASGEELHGAHQKGVLVVEGPLPPGFMQTVWRDDDRFVNTYWKSVPGQSVYSSF
EEEEEECCCCCHHCCCCCCCEEEEECCCCCHHHHHHHCCCCHHHHHHHHCCCCHHHHHHC
DWGIRDEDGYYYILGRTDDVINVAGHRLGTREIEESVSSHANVAEVAVVGVADQLKGQVA
CCCCCCCCCEEEEEECCCCHHHHHHCCCCHHHHHHHHHCCCCEEHEEEEECHHHCCCCEE
VAFVVPKDARGLEEPQARLKLEGDIMKTVDQQLGAVARPARVHFVTLLPKTRSGKLLRRA
EEEEECCCCCCCCCCHHHEEECCHHHHHHHHHHHHHCCCCEEEEEEECCCCCCCHHHHHH
LQAVAEGRDPGDLTTIEDPTALQAIRELLGR
HHHHHCCCCCCCCCCCCCCHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9006051; 10411265; 11677609; 10482501 [H]