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The map label for this gene is purK [H]

Identifier: 124265489

GI number: 124265489

Start: 320634

End: 321827

Strand: Direct

Name: purK [H]

Synonym: Mpe_A0296

Alternate gene names: 124265489

Gene position: 320634-321827 (Clockwise)

Preceding gene: 124265488

Following gene: 124265490

Centisome position: 7.93

GC content: 71.94

Gene sequence:

>1194_bases
ATGTCGAGCCACCACAAGACCCTTCTGCCCGCGGCCGTTGCCGCCGGTGCCCAGGCCAGCACACTCGGCGTGATGGGCGG
CGGCCAACTGGGCCGCATGTTCGTGCACGCCGCGCAGCGCCTGGGTTACTTCACCGCCGTGCTCGACCCGGACGTGGCGA
GCCCGGCCGGCCTCGTGTCGCACGTGCATGTGCAGACCGACTACCTCGACGAGCAGGGCCTGGCGCAGATGCTGCAGCGC
TGCGCCGCGATCACCACCGAGTTCGAGAACGTGCCGGCCGGCGCGCTCTACACGCTGGGGGCGCACCTGCCGGCGGCGCC
GGGCGCCGAGGCGGTGGCGGTGTGCCAGGACCGCGCGCGCGAGAAGCGGCATTTCGAGCAGTGCGGCGTGCCATGTGCGC
CGCATGCGCTCATCGAGACGCCCGAGCAGCTGGCCGGGGTCGATGCGGCGTTGTTGCCGGGCATCCTGAAGACCGCTCGC
CTGGGCTATGACGGCAAGGGCCAGCGCCGCGTGGCGACCCGCGCCGAGCTCGCACTCGCCTGGGACGAACTCAAGCGCGC
GCCCTGCGTGCTCGAGAGGCTGCTGCCGCTGGCCTTCGAGATCAGCGTGATCCTGGCCCGCAATGCTGACGGCGAGCTGG
TCTCCTTGCCGGTGCAGCAGAACCTGCACCGCGACGGCATCCTGGCGGTGACGCAAGTCCCGGCGCCGAACGTCCCCGCG
GGGCTGTCCGCAGCGGCGCGCGATGCGGCCGGTCGCATCGCCGAAGGTCTGGGCTACGTCGGTGTGCTGTGCGTCGAGTT
CTTCGTGCTCCAGGACGGCACGCTGGTGGTCAACGAGATGGCGCCGCGGCCGCACAACTCAGGCCACTACAGCATCGATG
CCTGCGACGTGTCGCAGTTCGAACTGCAGGTGCGCTGCATGACCGGGCTGCCGCTGGTCGCGCCGCGCCTGCATTCCCCG
GCGGTCATGCTCAACCTGCTCGGCGACCTGTGGTTTGATGCCGACGGCCGCGAGCGCACGCCCGACTGGGCCGCCGTGCT
GGCGCTGCCGGGCGCGCACCTGCACCTGTACGGCAAGAGCAGCGCCCAGCGCGGTCGCAAGATGGGTCACCTGACGCTGA
CTGCCGACACGCCCGAGGCAGCGCGCGCGACCGCGCTGCGGGCGGCGGAGCGGCTCGGCCTGCCGGCGTTCTGA

Upstream 100 bases:

>100_bases
ACCGAGGCAGCGCGTGCGCAAAGCGCCGCCTTGTCGAAGCACTCACCCTCCTGACGTCTTGGCCGGCCCGCGACGGTCGG
TCCCGCTGAAAGCTCCGCCG

Downstream 100 bases:

>100_bases
AGAACATGCCGCGGCTCGACGGTCATGCCCCCGAGGCGGTCGCCGAAGCCGCCCGGCTGCTCGCGGCCGGACACCTCGTC
GCCTTTCCTACCGAAACGGT

Product: phosphoribosylaminoimidazole carboxylase ATPase subunit

Products: NA

Alternate protein names: N5-CAIR synthase; 5-(carboxyamino)imidazole ribonucleotide synthetase [H]

Number of amino acids: Translated: 397; Mature: 396

Protein sequence:

>397_residues
MSSHHKTLLPAAVAAGAQASTLGVMGGGQLGRMFVHAAQRLGYFTAVLDPDVASPAGLVSHVHVQTDYLDEQGLAQMLQR
CAAITTEFENVPAGALYTLGAHLPAAPGAEAVAVCQDRAREKRHFEQCGVPCAPHALIETPEQLAGVDAALLPGILKTAR
LGYDGKGQRRVATRAELALAWDELKRAPCVLERLLPLAFEISVILARNADGELVSLPVQQNLHRDGILAVTQVPAPNVPA
GLSAAARDAAGRIAEGLGYVGVLCVEFFVLQDGTLVVNEMAPRPHNSGHYSIDACDVSQFELQVRCMTGLPLVAPRLHSP
AVMLNLLGDLWFDADGRERTPDWAAVLALPGAHLHLYGKSSAQRGRKMGHLTLTADTPEAARATALRAAERLGLPAF

Sequences:

>Translated_397_residues
MSSHHKTLLPAAVAAGAQASTLGVMGGGQLGRMFVHAAQRLGYFTAVLDPDVASPAGLVSHVHVQTDYLDEQGLAQMLQR
CAAITTEFENVPAGALYTLGAHLPAAPGAEAVAVCQDRAREKRHFEQCGVPCAPHALIETPEQLAGVDAALLPGILKTAR
LGYDGKGQRRVATRAELALAWDELKRAPCVLERLLPLAFEISVILARNADGELVSLPVQQNLHRDGILAVTQVPAPNVPA
GLSAAARDAAGRIAEGLGYVGVLCVEFFVLQDGTLVVNEMAPRPHNSGHYSIDACDVSQFELQVRCMTGLPLVAPRLHSP
AVMLNLLGDLWFDADGRERTPDWAAVLALPGAHLHLYGKSSAQRGRKMGHLTLTADTPEAARATALRAAERLGLPAF
>Mature_396_residues
SSHHKTLLPAAVAAGAQASTLGVMGGGQLGRMFVHAAQRLGYFTAVLDPDVASPAGLVSHVHVQTDYLDEQGLAQMLQRC
AAITTEFENVPAGALYTLGAHLPAAPGAEAVAVCQDRAREKRHFEQCGVPCAPHALIETPEQLAGVDAALLPGILKTARL
GYDGKGQRRVATRAELALAWDELKRAPCVLERLLPLAFEISVILARNADGELVSLPVQQNLHRDGILAVTQVPAPNVPAG
LSAAARDAAGRIAEGLGYVGVLCVEFFVLQDGTLVVNEMAPRPHNSGHYSIDACDVSQFELQVRCMTGLPLVAPRLHSPA
VMLNLLGDLWFDADGRERTPDWAAVLALPGAHLHLYGKSSAQRGRKMGHLTLTADTPEAARATALRAAERLGLPAF

Specific function: Catalyzes the ATP-dependent conversion of 5- aminoimidazole ribonucleotide (AIR) and HCO(3)(-) to N5- carboxyaminoimidazole ribonucleotide (N5-CAIR) [H]

COG id: COG0026

COG function: function code F; Phosphoribosylaminoimidazole carboxylase (NCAIR synthetase)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ATP-grasp domain [H]

Homologues:

Organism=Escherichia coli, GI1786733, Length=369, Percent_Identity=36.5853658536585, Blast_Score=171, Evalue=6e-44,
Organism=Escherichia coli, GI1788155, Length=370, Percent_Identity=25.6756756756757, Blast_Score=92, Evalue=4e-20,
Organism=Saccharomyces cerevisiae, GI6324702, Length=374, Percent_Identity=33.4224598930481, Blast_Score=206, Evalue=7e-54,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006115
- InterPro:   IPR005875
- InterPro:   IPR011761
- InterPro:   IPR003135
- InterPro:   IPR013815
- InterPro:   IPR013816
- InterPro:   IPR013817
- InterPro:   IPR016185
- InterPro:   IPR011054 [H]

Pfam domain/function: PF02222 ATP-grasp; PF03446 NAD_binding_2 [H]

EC number: =6.3.4.18 [H]

Molecular weight: Translated: 42043; Mature: 41912

Theoretical pI: Translated: 6.67; Mature: 6.67

Prosite motif: PS50975 ATP_GRASP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSSHHKTLLPAAVAAGAQASTLGVMGGGQLGRMFVHAAQRLGYFTAVLDPDVASPAGLVS
CCCCCCHHHHHHHHCCCCCCEEEECCCCHHHHHHHHHHHHHHHEEEEECCCCCCCHHHHH
HVHVQTDYLDEQGLAQMLQRCAAITTEFENVPAGALYTLGAHLPAAPGAEAVAVCQDRAR
EEEEEHHHCCHHHHHHHHHHHHHHHHHHCCCCCCHHHECCCCCCCCCCCHHHHHHHHHHH
EKRHFEQCGVPCAPHALIETPEQLAGVDAALLPGILKTARLGYDGKGQRRVATRAELALA
HHHHHHHCCCCCCCHHHHCCHHHHHCCCHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHH
WDELKRAPCVLERLLPLAFEISVILARNADGELVSLPVQQNLHRDGILAVTQVPAPNVPA
HHHHHCCHHHHHHHHHHHHEEEEEEEECCCCCEEECCHHHCCCCCCEEEEEECCCCCCCC
GLSAAARDAAGRIAEGLGYVGVLCVEFFVLQDGTLVVNEMAPRPHNSGHYSIDACDVSQF
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCCCCCEEEEECCCCEE
ELQVRCMTGLPLVAPRLHSPAVMLNLLGDLWFDADGRERTPDWAAVLALPGAHLHLYGKS
EEEEEEECCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCEEEEECCCCEEEEEECC
SAQRGRKMGHLTLTADTPEAARATALRAAERLGLPAF
HHHCCCCCCEEEEECCCCHHHHHHHHHHHHHCCCCCC
>Mature Secondary Structure 
SSHHKTLLPAAVAAGAQASTLGVMGGGQLGRMFVHAAQRLGYFTAVLDPDVASPAGLVS
CCCCCHHHHHHHHCCCCCCEEEECCCCHHHHHHHHHHHHHHHEEEEECCCCCCCHHHHH
HVHVQTDYLDEQGLAQMLQRCAAITTEFENVPAGALYTLGAHLPAAPGAEAVAVCQDRAR
EEEEEHHHCCHHHHHHHHHHHHHHHHHHCCCCCCHHHECCCCCCCCCCCHHHHHHHHHHH
EKRHFEQCGVPCAPHALIETPEQLAGVDAALLPGILKTARLGYDGKGQRRVATRAELALA
HHHHHHHCCCCCCCHHHHCCHHHHHCCCHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHH
WDELKRAPCVLERLLPLAFEISVILARNADGELVSLPVQQNLHRDGILAVTQVPAPNVPA
HHHHHCCHHHHHHHHHHHHEEEEEEEECCCCCEEECCHHHCCCCCCEEEEEECCCCCCCC
GLSAAARDAAGRIAEGLGYVGVLCVEFFVLQDGTLVVNEMAPRPHNSGHYSIDACDVSQF
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCCCCCEEEEECCCCEE
ELQVRCMTGLPLVAPRLHSPAVMLNLLGDLWFDADGRERTPDWAAVLALPGAHLHLYGKS
EEEEEEECCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCEEEEECCCCEEEEEECC
SAQRGRKMGHLTLTADTPEAARATALRAAERLGLPAF
HHHCCCCCCEEEEECCCCHHHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 3036807; 9384377 [H]