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The map label for this gene is hisJ [H]

Identifier: 124265324

GI number: 124265324

Start: 135211

End: 136068

Strand: Direct

Name: hisJ [H]

Synonym: Mpe_A0131

Alternate gene names: 124265324

Gene position: 135211-136068 (Clockwise)

Preceding gene: 124265323

Following gene: 124265325

Centisome position: 3.34

GC content: 66.08

Gene sequence:

>858_bases
ATGAAGCTCACGACCCGCCAGTTTCTGAAGACCGCCGGCCTGTTCGGTGCCGGCCTGCTGCTCGCCGCCTGCGGCAAGAA
GGAAGAGCCGGCGCCCGCCGCCGCCCCCGCTTCCGCGCCCGCGCCGGTGGCTTCCGCTCCGGCGCCCGCGCCCGCCAAGG
TCTACGTCGTCGGCACCGATGCCGCCTACGCCCCGTTCGAGTTCCAGAACGAGAAGGGCGAGATCGTCGGCTTCAGCATC
GACCTGCTCCAGGCCGTGGCCGCCAAGGGCGGCTTCGAGGTGAAGTTCGTCAACACGCCGTGGGAAGGCATCTTCAACGC
GCTGCAGCAGGGTGACCGCGACCTGCTGATCTCGTCGATCACCATCACCGACGAGCGCAAGCAGACGATGGACTTCTCCA
ACCCCTACTTCGACGCCTTCCAGCTGATCGCGGTGAAGGCCACCTCCAAGGTCGCGAAGTTCGACGACCTGAAGAAGCTC
AAGGTCGGCGTGCAGACCGGCACCACCGGCGACGAGGTCGTGACCAAGCTGCAGGGCAAGAACAGCGCCAACGTCAAGCG
CTTCGAGTCGACGCCGCTGGCGTTGAAGGAGCTCGAGGCGGGCGGTGTCGATGCCGTCGTGGCCGACAACGGCGTGATCA
TCAACTACGTCACCAACAACGCGGGCGCCAACTTCAAGACCGTCAGCGACAGCGCCTTCGCGCCCGAGCAGTACGGCATC
GCCGTCAAGAAGGGCAACGCCGAACTGCTGGAGAAGGTGAACAAGGGCATCGCGGCCATCAAGGCCGACGGCAGCTACGA
CCAGGTCTACGCCAAGACCTTCGGTGCAGCCGCTCCCGCGGCGGCGGCTTCCAAGTAA

Upstream 100 bases:

>100_bases
AAAGCGGCCTCCGGGCCGCTTTTTTGCATCAACAATCCGGTGACAATCGGTCACCGCCGATGTGTCGCCATCGATTCCGG
AGCCCCCACGAGGATCCCGC

Downstream 100 bases:

>100_bases
GCAGCGGCTTCAGGACCCCGAGGATCTCCCATGGACTTTCGCTGGGAGATCCTGGCCGGCTACGGACCGCTGTTCCTGGC
CGGCGTCCTGATGACGATCA

Product: glutamine transport system substrate-binding protein

Products: NA

Alternate protein names: HBP [H]

Number of amino acids: Translated: 285; Mature: 285

Protein sequence:

>285_residues
MKLTTRQFLKTAGLFGAGLLLAACGKKEEPAPAAAPASAPAPVASAPAPAPAKVYVVGTDAAYAPFEFQNEKGEIVGFSI
DLLQAVAAKGGFEVKFVNTPWEGIFNALQQGDRDLLISSITITDERKQTMDFSNPYFDAFQLIAVKATSKVAKFDDLKKL
KVGVQTGTTGDEVVTKLQGKNSANVKRFESTPLALKELEAGGVDAVVADNGVIINYVTNNAGANFKTVSDSAFAPEQYGI
AVKKGNAELLEKVNKGIAAIKADGSYDQVYAKTFGAAAPAAAASK

Sequences:

>Translated_285_residues
MKLTTRQFLKTAGLFGAGLLLAACGKKEEPAPAAAPASAPAPVASAPAPAPAKVYVVGTDAAYAPFEFQNEKGEIVGFSI
DLLQAVAAKGGFEVKFVNTPWEGIFNALQQGDRDLLISSITITDERKQTMDFSNPYFDAFQLIAVKATSKVAKFDDLKKL
KVGVQTGTTGDEVVTKLQGKNSANVKRFESTPLALKELEAGGVDAVVADNGVIINYVTNNAGANFKTVSDSAFAPEQYGI
AVKKGNAELLEKVNKGIAAIKADGSYDQVYAKTFGAAAPAAAASK
>Mature_285_residues
MKLTTRQFLKTAGLFGAGLLLAACGKKEEPAPAAAPASAPAPVASAPAPAPAKVYVVGTDAAYAPFEFQNEKGEIVGFSI
DLLQAVAAKGGFEVKFVNTPWEGIFNALQQGDRDLLISSITITDERKQTMDFSNPYFDAFQLIAVKATSKVAKFDDLKKL
KVGVQTGTTGDEVVTKLQGKNSANVKRFESTPLALKELEAGGVDAVVADNGVIINYVTNNAGANFKTVSDSAFAPEQYGI
AVKKGNAELLEKVNKGIAAIKADGSYDQVYAKTFGAAAPAAAASK

Specific function: Component of the high-affinity histidine permease, a binding-protein-dependent transport system. The other components are proteins hisQ, hisM, and hisP [H]

COG id: COG0834

COG function: function code ET; ABC-type amino acid transport/signal transduction systems, periplasmic component/domain

Gene ontology:

Cell location: Cell membrane; Lipid-anchor (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 3 family [H]

Homologues:

Organism=Escherichia coli, GI1788228, Length=225, Percent_Identity=34.2222222222222, Blast_Score=129, Evalue=2e-31,
Organism=Escherichia coli, GI1787031, Length=230, Percent_Identity=35.6521739130435, Blast_Score=124, Evalue=7e-30,
Organism=Escherichia coli, GI1787085, Length=220, Percent_Identity=33.1818181818182, Blast_Score=110, Evalue=1e-25,
Organism=Escherichia coli, GI1787088, Length=218, Percent_Identity=31.1926605504587, Blast_Score=105, Evalue=3e-24,
Organism=Escherichia coli, GI1788649, Length=237, Percent_Identity=30.379746835443, Blast_Score=105, Evalue=4e-24,
Organism=Escherichia coli, GI1788648, Length=224, Percent_Identity=28.5714285714286, Blast_Score=100, Evalue=1e-22,

Paralogues:

None

Copy number: 1920 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1060 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 80 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015683
- InterPro:   IPR001638
- InterPro:   IPR018313 [H]

Pfam domain/function: PF00497 SBP_bac_3 [H]

EC number: NA

Molecular weight: Translated: 29771; Mature: 29771

Theoretical pI: Translated: 8.36; Mature: 8.36

Prosite motif: PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
0.7 %Met     (Translated Protein)
1.1 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
0.7 %Met     (Mature Protein)
1.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLTTRQFLKTAGLFGAGLLLAACGKKEEPAPAAAPASAPAPVASAPAPAPAKVYVVGTD
CCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEEECC
AAYAPFEFQNEKGEIVGFSIDLLQAVAAKGGFEVKFVNTPWEGIFNALQQGDRDLLISSI
CCCCCEEEECCCCCEEEEHHHHHHHHHHCCCEEEEEECCCHHHHHHHHHCCCCEEEEEEE
TITDERKQTMDFSNPYFDAFQLIAVKATSKVAKFDDLKKLKVGVQTGTTGDEVVTKLQGK
EEECCHHHHCCCCCCHHHHEEEEEEHHHHHHHHHHHHHHHEEEECCCCCHHHHHHHHCCC
NSANVKRFESTPLALKELEAGGVDAVVADNGVIINYVTNNAGANFKTVSDSAFAPEQYGI
CCCCCEEECCCCHHHHHHCCCCCEEEEECCCEEEEEEECCCCCCEEECCCCCCCCHHHCE
AVKKGNAELLEKVNKGIAAIKADGSYDQVYAKTFGAAAPAAAASK
EEECCCHHHHHHHCCCEEEEECCCCHHHHHHHHHCCCCCCCCCCC
>Mature Secondary Structure
MKLTTRQFLKTAGLFGAGLLLAACGKKEEPAPAAAPASAPAPVASAPAPAPAKVYVVGTD
CCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEEECC
AAYAPFEFQNEKGEIVGFSIDLLQAVAAKGGFEVKFVNTPWEGIFNALQQGDRDLLISSI
CCCCCEEEECCCCCEEEEHHHHHHHHHHCCCEEEEEECCCHHHHHHHHHCCCCEEEEEEE
TITDERKQTMDFSNPYFDAFQLIAVKATSKVAKFDDLKKLKVGVQTGTTGDEVVTKLQGK
EEECCHHHHCCCCCCHHHHEEEEEEHHHHHHHHHHHHHHHEEEECCCCCHHHHHHHHCCC
NSANVKRFESTPLALKELEAGGVDAVVADNGVIINYVTNNAGANFKTVSDSAFAPEQYGI
CCCCCEEECCCCHHHHHHCCCCCEEEEECCCEEEEEEECCCCCCEEECCCCCCCCHHHCE
AVKKGNAELLEKVNKGIAAIKADGSYDQVYAKTFGAAAPAAAASK
EEECCCHHHHHHHCCCEEEEECCCCHHHHHHHHHCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 1448614 [H]