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The map label for this gene is cysP [H]

Identifier: 124265315

GI number: 124265315

Start: 124475

End: 125488

Strand: Direct

Name: cysP [H]

Synonym: Mpe_A0122

Alternate gene names: 124265315

Gene position: 124475-125488 (Clockwise)

Preceding gene: 124265314

Following gene: 124265316

Centisome position: 3.08

GC content: 65.09

Gene sequence:

>1014_bases
ATGACCCGTTTCGCTTCCGTCCGTGGCGCGCTCGCCGCCTCGATCCTTGTTTTGGCCGCCGGTGCCGCCGCCGCCAAGGA
CGTGACCCTGCTCAATGTCTCCTACGACCCGACGCGCGAGCTCTACGTCGAGTACAACGCCGCCTTCGCCAAGTACTGGA
AGGGCAAGACCGGCGACAACGTGACCGTGAAGCAGTCGCACGGCGGCTCGGGCAAGCAGGCTCGCTCGGTGATCGACGGC
ATCGACGCCGACGTGGTGACGCTGGCGCTGGCCTACGACATCGACGAGATCGGCGAGAAGGCCAAGCTGCTGCCGGCCGA
CTGGCAGAAGCGCCTGAAGCACAACAGCTCGCCCTACACCTCCACCTACATCTTCCTGGTGCGCAAGGGCAACCCGAAGG
GCATCAAGAACTGGGACGACCTGGTGAAGCCGGGCGTGTCGGTGATCACCGCGAACCCCAAGACCTCGGGTGGCGCCCGC
TGGGGCTACCTGGCGGCCTACGGCTTCGCGCTCAAGCAGCCCGGAGGCGACGATGCCAAGGCGCGCGAGTTCGTCGGCAA
CCTGTTCAAGAACGTGCCGGTGCTGGATTCCGGCGCCCGCGGCTCCACGGTGACCTTCGCCGAGCGTGGTATCGGTGACG
TGCTGCTGGCCTGGGAGAACGAGGCTCACCTTTCGCTGAAGGAGTTCGGCGTCGACAAGTTCGACATCGTCTACCCGCCG
CTGAGCATCCTGGCCGAGCCGCCGGTGACGGTGGTCGACAAGGTGGTCGACAAGAAGGGCACCCGCGACGTGGCCCAGGC
CTACCTCGAGTACCTGTACACCGCGGAGGGCCAGGAAATCGCCGCGCGCAACTTCTACCGGCCGATCGACGAGAAGGTCG
CGGCGAAGTACGCGAAGAACTTCCCGAAGGTCAACCTGTTCACCATCGACGAGGTGTTCGGCGGGTGGGCCAAGGCGCAG
AAGACCCACTTCGCCGACGGCGGTGTGTTCGATCAGATCTATACGAAGAAGTAA

Upstream 100 bases:

>100_bases
TAAGCGTCCTTGCTTTCATTCGTTCCGCGTTGAAGCGGCGCTTTCTAGCATCTCCCCATTCGCTCATTCCCGCCCTGATT
CCGTGATCCCGAGGACCTTC

Downstream 100 bases:

>100_bases
GCACCGGGATTTTTTCGAGAGCAGGCGCCCACTTGCTGGGCGCCTGTCGGCGTTTGGCTACGCCATTTTTCTCATAAGCA
TTCGACGAATCTGTCGTTCC

Product: thiosulfate binding protein

Products: ADP; phosphate; S2O32- [Cytoplasm]; SO42- [Cytoplasm] [C]

Alternate protein names: Sulfate starvation-induced protein 2; SSI2 [H]

Number of amino acids: Translated: 337; Mature: 336

Protein sequence:

>337_residues
MTRFASVRGALAASILVLAAGAAAAKDVTLLNVSYDPTRELYVEYNAAFAKYWKGKTGDNVTVKQSHGGSGKQARSVIDG
IDADVVTLALAYDIDEIGEKAKLLPADWQKRLKHNSSPYTSTYIFLVRKGNPKGIKNWDDLVKPGVSVITANPKTSGGAR
WGYLAAYGFALKQPGGDDAKAREFVGNLFKNVPVLDSGARGSTVTFAERGIGDVLLAWENEAHLSLKEFGVDKFDIVYPP
LSILAEPPVTVVDKVVDKKGTRDVAQAYLEYLYTAEGQEIAARNFYRPIDEKVAAKYAKNFPKVNLFTIDEVFGGWAKAQ
KTHFADGGVFDQIYTKK

Sequences:

>Translated_337_residues
MTRFASVRGALAASILVLAAGAAAAKDVTLLNVSYDPTRELYVEYNAAFAKYWKGKTGDNVTVKQSHGGSGKQARSVIDG
IDADVVTLALAYDIDEIGEKAKLLPADWQKRLKHNSSPYTSTYIFLVRKGNPKGIKNWDDLVKPGVSVITANPKTSGGAR
WGYLAAYGFALKQPGGDDAKAREFVGNLFKNVPVLDSGARGSTVTFAERGIGDVLLAWENEAHLSLKEFGVDKFDIVYPP
LSILAEPPVTVVDKVVDKKGTRDVAQAYLEYLYTAEGQEIAARNFYRPIDEKVAAKYAKNFPKVNLFTIDEVFGGWAKAQ
KTHFADGGVFDQIYTKK
>Mature_336_residues
TRFASVRGALAASILVLAAGAAAAKDVTLLNVSYDPTRELYVEYNAAFAKYWKGKTGDNVTVKQSHGGSGKQARSVIDGI
DADVVTLALAYDIDEIGEKAKLLPADWQKRLKHNSSPYTSTYIFLVRKGNPKGIKNWDDLVKPGVSVITANPKTSGGARW
GYLAAYGFALKQPGGDDAKAREFVGNLFKNVPVLDSGARGSTVTFAERGIGDVLLAWENEAHLSLKEFGVDKFDIVYPPL
SILAEPPVTVVDKVVDKKGTRDVAQAYLEYLYTAEGQEIAARNFYRPIDEKVAAKYAKNFPKVNLFTIDEVFGGWAKAQK
THFADGGVFDQIYTKK

Specific function: This protein specifically binds sulfate and is involved in its transmembrane transport [H]

COG id: COG1613

COG function: function code P; ABC-type sulfate transport system, periplasmic component

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the prokaryotic sulfate-binding protein family [H]

Homologues:

Organism=Escherichia coli, GI1790351, Length=318, Percent_Identity=68.8679245283019, Blast_Score=453, Evalue=1e-129,
Organism=Escherichia coli, GI1788765, Length=305, Percent_Identity=48.1967213114754, Blast_Score=293, Evalue=8e-81,

Paralogues:

None

Copy number: 1240 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006059
- InterPro:   IPR000957
- InterPro:   IPR005669 [H]

Pfam domain/function: PF01547 SBP_bac_1 [H]

EC number: NA

Molecular weight: Translated: 36740; Mature: 36609

Theoretical pI: Translated: 9.43; Mature: 9.43

Prosite motif: PS00401 PROK_SULFATE_BIND_1 ; PS00757 PROK_SULFATE_BIND_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
0.3 %Met     (Translated Protein)
0.3 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
0.0 %Met     (Mature Protein)
0.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTRFASVRGALAASILVLAAGAAAAKDVTLLNVSYDPTRELYVEYNAAFAKYWKGKTGDN
CCCHHHHHHHHHHHHHHHHCCCCHHCCEEEEEECCCCCEEEEEEECHHHHHHHCCCCCCC
VTVKQSHGGSGKQARSVIDGIDADVVTLALAYDIDEIGEKAKLLPADWQKRLKHNSSPYT
EEEECCCCCCCHHHHHHHHCCCHHHHHHHHHHCHHHHCCCCCCCCHHHHHHHHCCCCCCC
STYIFLVRKGNPKGIKNWDDLVKPGVSVITANPKTSGGARWGYLAAYGFALKQPGGDDAK
EEEEEEEECCCCCCCCCHHHHHCCCCEEEECCCCCCCCCCCHHHHHHHHHCCCCCCCCHH
AREFVGNLFKNVPVLDSGARGSTVTFAERGIGDVLLAWENEAHLSLKEFGVDKFDIVYPP
HHHHHHHHHHCCCCCCCCCCCCEEEEHHCCCCEEEEEECCCCCEEHHHHCCCCEEEECCC
LSILAEPPVTVVDKVVDKKGTRDVAQAYLEYLYTAEGQEIAARNFYRPIDEKVAAKYAKN
HHHHCCCCHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCHHHHHHHCCCHHHHHHHHHHHC
FPKVNLFTIDEVFGGWAKAQKTHFADGGVFDQIYTKK
CCCEEEEEHHHHHCCHHHHHHCCCCCCCCHHHHHCCC
>Mature Secondary Structure 
TRFASVRGALAASILVLAAGAAAAKDVTLLNVSYDPTRELYVEYNAAFAKYWKGKTGDN
CCHHHHHHHHHHHHHHHHCCCCHHCCEEEEEECCCCCEEEEEEECHHHHHHHCCCCCCC
VTVKQSHGGSGKQARSVIDGIDADVVTLALAYDIDEIGEKAKLLPADWQKRLKHNSSPYT
EEEECCCCCCCHHHHHHHHCCCHHHHHHHHHHCHHHHCCCCCCCCHHHHHHHHCCCCCCC
STYIFLVRKGNPKGIKNWDDLVKPGVSVITANPKTSGGARWGYLAAYGFALKQPGGDDAK
EEEEEEEECCCCCCCCCHHHHHCCCCEEEECCCCCCCCCCCHHHHHHHHHCCCCCCCCHH
AREFVGNLFKNVPVLDSGARGSTVTFAERGIGDVLLAWENEAHLSLKEFGVDKFDIVYPP
HHHHHHHHHHCCCCCCCCCCCCEEEEHHCCCCEEEEEECCCCCEEHHHHCCCCEEEECCC
LSILAEPPVTVVDKVVDKKGTRDVAQAYLEYLYTAEGQEIAARNFYRPIDEKVAAKYAKN
HHHHCCCCHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCHHHHHHHCCCHHHHHHHHHHHC
FPKVNLFTIDEVFGGWAKAQKTHFADGGVFDQIYTKK
CCCEEEEEHHHHHCCHHHHHHCCCCCCCCHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; S2O32- [Periplasm]; H2O; SO42- [Periplasm]; ATP [C]

Specific reaction: ATP + S2O32- [Periplasm] + H2O = ADP + phosphate + S2O32- [Cytoplasm] SO42- [Periplasm] + H2O + ATP = SO42- [Cytoplasm] + phosphate + ADP [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 3158524; 2002055; 8346018; 9278503; 9298646; 8774726 [H]