Click here to switch to the map view.

The map label for this gene is cysP [H]

Identifier: 124265316

GI number: 124265316

Start: 125660

End: 126673

Strand: Direct

Name: cysP [H]

Synonym: Mpe_A0123

Alternate gene names: 124265316

Gene position: 125660-126673 (Clockwise)

Preceding gene: 124265315

Following gene: 124265317

Centisome position: 3.11

GC content: 66.07

Gene sequence:

>1014_bases
ATGTCTCTCCTGCCTTCGTCGCTGCGGCGCGCACTGATCGTTTCTCCCCTGTTGCTGGCCGCCGGCTGGGCCACGGCCGC
CGGGCCGACGCTGCTGAACGTCAGCTACGACGTGGCGCGCGAGTTCTACAAGGAGATCAACCCGGCCTTCCAGAAACAAT
GGAAGCAGGCCAGCGGCGAGGACGTGACGATCAACCAATCCCACGGCGGCTCGAGCAAGCAGGCCCGCGCGGTGATCGAC
GGTCTGGAGGCCGACGTCGTGACGATGAACCAGTCGAGCGACATCGACGCCATCGCCACCCGCGGCAAGCTGATCCCCGA
AGACTGGGCGAAGCGGCTGCCGAACAACGCCGCGCCCACCACCTCGGTCACGGTGATCCTGGTGCGCAAGGGCAACCCGA
AGAAGATCGCCGACTGGCCCGATCTCGCCAAGCCCGGCGTCAGCGTTGTGATCCCGAATCCCAAGGTCACCGGCAACGGT
CGCTACAGCTATGTGGCCGCCTGGGGCTCGGCCATCAAGGCCGGCGGTACGCCGGCCCAGGCGCGCGAGCTGGTGCAGAA
GATCTTCGCCAACGTGCCGGTGTTCGACGGTGGCGGCCGTGCGGCGACCACCACCTTCGCACAGCGCAACATCGGCGACG
CGCTGGCGACCTTCGAGTCGGAGGTCAACCTGATCAAGGCCGAGTTCGGCGACAACTTCGACGTGGTCTACCCCAAATCG
ACCATCCTGGCCGAGAACCCGGTGAGCGTGATCGACACCGTGGTCGACAAGAAGGGCACGCGCAAGCAGGCCGAGGCCTA
CCTGAAGTTCCTGTGGTCCGACGAGGCCCAGGAGATCGCCGCCAAGCACCAGTTGCGCCCGCGCTCGCCCGCGCTGCTGG
CGAAGTACGCGAAGGACTTCCCGAAGGTCAACACCTTCACGATCGACGAGATCTTCGGCGGCTGGAGCAAGGCGCAGAAG
GAGCACTTCGACGACGGCGGGATCTACGACCAGATCCTGGCGGCGCGCAAGTGA

Upstream 100 bases:

>100_bases
TCATAAGCATTCGACGAATCTGTCGTTCCCGTTTTAAGCGACGGTCCCTAGCATCCGCTCCCATCGACCTTTCCGCGGGG
ACTCCGGGCCCTGCCACATC

Downstream 100 bases:

>100_bases
TCGTGCCGGTTCGATCATTTTCCTGAAGTACTGACCGTGTTCCTCTCTCGCGCCCTGCTGCGGCGACACAGCGTGCTGCC
CGGCTTCGACCTCGCGCTCG

Product: thiosulfate binding protein

Products: ADP; phosphate; S2O32- [Cytoplasm]; SO42- [Cytoplasm] [C]

Alternate protein names: Sulfate starvation-induced protein 2; SSI2 [H]

Number of amino acids: Translated: 337; Mature: 336

Protein sequence:

>337_residues
MSLLPSSLRRALIVSPLLLAAGWATAAGPTLLNVSYDVAREFYKEINPAFQKQWKQASGEDVTINQSHGGSSKQARAVID
GLEADVVTMNQSSDIDAIATRGKLIPEDWAKRLPNNAAPTTSVTVILVRKGNPKKIADWPDLAKPGVSVVIPNPKVTGNG
RYSYVAAWGSAIKAGGTPAQARELVQKIFANVPVFDGGGRAATTTFAQRNIGDALATFESEVNLIKAEFGDNFDVVYPKS
TILAENPVSVIDTVVDKKGTRKQAEAYLKFLWSDEAQEIAAKHQLRPRSPALLAKYAKDFPKVNTFTIDEIFGGWSKAQK
EHFDDGGIYDQILAARK

Sequences:

>Translated_337_residues
MSLLPSSLRRALIVSPLLLAAGWATAAGPTLLNVSYDVAREFYKEINPAFQKQWKQASGEDVTINQSHGGSSKQARAVID
GLEADVVTMNQSSDIDAIATRGKLIPEDWAKRLPNNAAPTTSVTVILVRKGNPKKIADWPDLAKPGVSVVIPNPKVTGNG
RYSYVAAWGSAIKAGGTPAQARELVQKIFANVPVFDGGGRAATTTFAQRNIGDALATFESEVNLIKAEFGDNFDVVYPKS
TILAENPVSVIDTVVDKKGTRKQAEAYLKFLWSDEAQEIAAKHQLRPRSPALLAKYAKDFPKVNTFTIDEIFGGWSKAQK
EHFDDGGIYDQILAARK
>Mature_336_residues
SLLPSSLRRALIVSPLLLAAGWATAAGPTLLNVSYDVAREFYKEINPAFQKQWKQASGEDVTINQSHGGSSKQARAVIDG
LEADVVTMNQSSDIDAIATRGKLIPEDWAKRLPNNAAPTTSVTVILVRKGNPKKIADWPDLAKPGVSVVIPNPKVTGNGR
YSYVAAWGSAIKAGGTPAQARELVQKIFANVPVFDGGGRAATTTFAQRNIGDALATFESEVNLIKAEFGDNFDVVYPKST
ILAENPVSVIDTVVDKKGTRKQAEAYLKFLWSDEAQEIAAKHQLRPRSPALLAKYAKDFPKVNTFTIDEIFGGWSKAQKE
HFDDGGIYDQILAARK

Specific function: This protein specifically binds sulfate and is involved in its transmembrane transport [H]

COG id: COG1613

COG function: function code P; ABC-type sulfate transport system, periplasmic component

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the prokaryotic sulfate-binding protein family [H]

Homologues:

Organism=Escherichia coli, GI1790351, Length=318, Percent_Identity=55.3459119496855, Blast_Score=349, Evalue=1e-97,
Organism=Escherichia coli, GI1788765, Length=308, Percent_Identity=49.3506493506493, Blast_Score=309, Evalue=2e-85,

Paralogues:

None

Copy number: 1240 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006059
- InterPro:   IPR000957
- InterPro:   IPR005669 [H]

Pfam domain/function: PF01547 SBP_bac_1 [H]

EC number: NA

Molecular weight: Translated: 36510; Mature: 36379

Theoretical pI: Translated: 9.68; Mature: 9.68

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
0.6 %Met     (Translated Protein)
0.6 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
0.3 %Met     (Mature Protein)
0.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSLLPSSLRRALIVSPLLLAAGWATAAGPTLLNVSYDVAREFYKEINPAFQKQWKQASGE
CCCCHHHHHHHHHHHHHHHHHCCCHHCCCEEEECCHHHHHHHHHHCCHHHHHHHHHCCCC
DVTINQSHGGSSKQARAVIDGLEADVVTMNQSSDIDAIATRGKLIPEDWAKRLPNNAAPT
EEEEECCCCCCCHHHHHHHHCCCCCEEEECCCCCCHHHHHCCCCCCHHHHHHCCCCCCCC
TSVTVILVRKGNPKKIADWPDLAKPGVSVVIPNPKVTGNGRYSYVAAWGSAIKAGGTPAQ
CEEEEEEEECCCCCCCCCCCCCCCCCCEEEECCCEECCCCCEEEEEEHHHHHHCCCCHHH
ARELVQKIFANVPVFDGGGRAATTTFAQRNIGDALATFESEVNLIKAEFGDNFDVVYPKS
HHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHEEEECCCCCCEEECCCC
TILAENPVSVIDTVVDKKGTRKQAEAYLKFLWSDEAQEIAAKHQLRPRSPALLAKYAKDF
CEECCCCHHHHHHHHCCCCCHHHHHHHHHHHHCCHHHHHHHHHCCCCCCHHHHHHHHHHC
PKVNTFTIDEIFGGWSKAQKEHFDDGGIYDQILAARK
CCCCEEEHHHHHCCHHHHHHHHCCCCCHHHHHHHCCC
>Mature Secondary Structure 
SLLPSSLRRALIVSPLLLAAGWATAAGPTLLNVSYDVAREFYKEINPAFQKQWKQASGE
CCCHHHHHHHHHHHHHHHHHCCCHHCCCEEEECCHHHHHHHHHHCCHHHHHHHHHCCCC
DVTINQSHGGSSKQARAVIDGLEADVVTMNQSSDIDAIATRGKLIPEDWAKRLPNNAAPT
EEEEECCCCCCCHHHHHHHHCCCCCEEEECCCCCCHHHHHCCCCCCHHHHHHCCCCCCCC
TSVTVILVRKGNPKKIADWPDLAKPGVSVVIPNPKVTGNGRYSYVAAWGSAIKAGGTPAQ
CEEEEEEEECCCCCCCCCCCCCCCCCCEEEECCCEECCCCCEEEEEEHHHHHHCCCCHHH
ARELVQKIFANVPVFDGGGRAATTTFAQRNIGDALATFESEVNLIKAEFGDNFDVVYPKS
HHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHEEEECCCCCCEEECCCC
TILAENPVSVIDTVVDKKGTRKQAEAYLKFLWSDEAQEIAAKHQLRPRSPALLAKYAKDF
CEECCCCHHHHHHHHCCCCCHHHHHHHHHHHHCCHHHHHHHHHCCCCCCHHHHHHHHHHC
PKVNTFTIDEIFGGWSKAQKEHFDDGGIYDQILAARK
CCCCEEEHHHHHCCHHHHHHHHCCCCCHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; S2O32- [Periplasm]; H2O; SO42- [Periplasm]; ATP [C]

Specific reaction: ATP + S2O32- [Periplasm] + H2O = ADP + phosphate + S2O32- [Cytoplasm] SO42- [Periplasm] + H2O + ATP = SO42- [Cytoplasm] + phosphate + ADP [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 3158524; 2002055; 8346018; 9278503; 9298646; 8774726 [H]