Click here to switch to the map view.

The map label for this gene is mdeA [H]

Identifier: 124265221

GI number: 124265221

Start: 29475

End: 30776

Strand: Reverse

Name: mdeA [H]

Synonym: Mpe_A0028

Alternate gene names: 124265221

Gene position: 30776-29475 (Counterclockwise)

Preceding gene: 124265222

Following gene: 124265220

Centisome position: 0.76

GC content: 69.28

Gene sequence:

>1302_bases
ATGAGCGACCGCCCGCCGTTGAAGTTCGAAACCCTGGCCGTGCACGGCGGCTACAAGCCCGACCCGACCACCAAGGCGGT
CGCGGTGCCGATCTACCAGACCGTGGCCTACGCGTTCGACAGCGCGCAGCACGGCGCCGACCTGTTCGACCTGAAGGTGC
CGGGCAACATCTACACGCGGATCATGAATCCGACGCAGGACGTGCTGGAGCAGCGCGTCGCGGCGCTCGAGGGCGGCATC
GCCGCGCTGGCGCTGGCCTCCGGCCAGGCCGCGGTCACGTATGCCATCCTGACCATCGCCGAGGCCGGCGACAACATCGT
GGCCTCCAGCGCGCTGTACGGCGGCACCTACAACCTGCTGGCGCACACGCTGCCGCAATACGGCATCGCCACGCGCTTCG
CCGACTACCGCGACCCGGCCTCGTTCGAGCCGCTGATCGACGGAAAGACCAAGGCGGTGTTCGTGGAATCGCTCGGCAAC
CCGCAGGGCAACATCACCGACATCGCGAAGATCGCCGAGGTGGCGCACCGCCACGGCGTGCCGCTGATCGTGGACAACAC
CGTGCCCTCGCCCTACCTGAGCCGGCCCTTCGAGCACGGCGCCGACATCGTGGTGCACTCGCTCACCAAGTACCTGGGCG
GCCACGGCAACAGCGTGGGCGGCGCGATCGTCGACTCGGGCAAGTTCCCGTGGGCACAGCACAAGGAACGCTTCAAGCGC
CTGAACGAGCCCGACGTCAGCTACCACGGCGTGGTCTACACCGACGCGCTGGGCCCGGCGGCCTACATCGGCCGCGCGCG
CGTGGTGCCGCTGCGCAACACCGGCGCGGCGATCTCGCCGTTCAACGCGTTCCTGATCCTGCAGGGCATCGAGACGCTGG
CGCTGCGCATGGACCGCATCTGCAGCAACGCGCTGGCGGTGGCGAAGTACCTGCAGCGCCACGCCAAGGTGGGCTGGGTC
AACTACGCCGGGCTCGAGGACCACCCCGACCACGCGCTGGTGCAGAAGTACCTGTCGGGCAAGGCTTCGGGGCTGCTGAC
CTTCGGCATCAAGAGCCCGGCCGGCGGTGGCCGCGAGGCCGGCGCGCGCTTCCTCGACGCGCTGGGCTTGTTCACGCGGC
TCGTGAACATCGGCGACGCGAAATCGTTGGCCACTCACCCGGCCTCGACCACCCACCGGCAACTCTCGCCCGAAGAGCTG
TTGAAGGCCGGCGTCGGCGAGGACACGGTGCGGCTGTCGCTGGGCATCGAGCACATCGACGACCTGCTGGCCGACCTGGA
GCAGGCGCTGGCCGGCGTGTAG

Upstream 100 bases:

>100_bases
GCGCGGGCTCCGGGGCGGTGTCGCTATGCGATAACCATAGTTCCCAATCCGCCTCCGAGCGCCTAGGCTGCCGGCTCCCG
CCACCCCCTGGAGCCGCCCG

Downstream 100 bases:

>100_bases
CGGCCCGCGGTCGCGCCGGCGTGGACGGCGGCGCATCCTTGCTTCCTGCATAAGCAAGCGCGCAGAGATTGGTTCCGGTT
CGGCCGCCAGCTCCCTACGC

Product: O-acetylhomoserine aminocarboxypropyltransferase

Products: NA

Alternate protein names: L-methioninase [H]

Number of amino acids: Translated: 433; Mature: 432

Protein sequence:

>433_residues
MSDRPPLKFETLAVHGGYKPDPTTKAVAVPIYQTVAYAFDSAQHGADLFDLKVPGNIYTRIMNPTQDVLEQRVAALEGGI
AALALASGQAAVTYAILTIAEAGDNIVASSALYGGTYNLLAHTLPQYGIATRFADYRDPASFEPLIDGKTKAVFVESLGN
PQGNITDIAKIAEVAHRHGVPLIVDNTVPSPYLSRPFEHGADIVVHSLTKYLGGHGNSVGGAIVDSGKFPWAQHKERFKR
LNEPDVSYHGVVYTDALGPAAYIGRARVVPLRNTGAAISPFNAFLILQGIETLALRMDRICSNALAVAKYLQRHAKVGWV
NYAGLEDHPDHALVQKYLSGKASGLLTFGIKSPAGGGREAGARFLDALGLFTRLVNIGDAKSLATHPASTTHRQLSPEEL
LKAGVGEDTVRLSLGIEHIDDLLADLEQALAGV

Sequences:

>Translated_433_residues
MSDRPPLKFETLAVHGGYKPDPTTKAVAVPIYQTVAYAFDSAQHGADLFDLKVPGNIYTRIMNPTQDVLEQRVAALEGGI
AALALASGQAAVTYAILTIAEAGDNIVASSALYGGTYNLLAHTLPQYGIATRFADYRDPASFEPLIDGKTKAVFVESLGN
PQGNITDIAKIAEVAHRHGVPLIVDNTVPSPYLSRPFEHGADIVVHSLTKYLGGHGNSVGGAIVDSGKFPWAQHKERFKR
LNEPDVSYHGVVYTDALGPAAYIGRARVVPLRNTGAAISPFNAFLILQGIETLALRMDRICSNALAVAKYLQRHAKVGWV
NYAGLEDHPDHALVQKYLSGKASGLLTFGIKSPAGGGREAGARFLDALGLFTRLVNIGDAKSLATHPASTTHRQLSPEEL
LKAGVGEDTVRLSLGIEHIDDLLADLEQALAGV
>Mature_432_residues
SDRPPLKFETLAVHGGYKPDPTTKAVAVPIYQTVAYAFDSAQHGADLFDLKVPGNIYTRIMNPTQDVLEQRVAALEGGIA
ALALASGQAAVTYAILTIAEAGDNIVASSALYGGTYNLLAHTLPQYGIATRFADYRDPASFEPLIDGKTKAVFVESLGNP
QGNITDIAKIAEVAHRHGVPLIVDNTVPSPYLSRPFEHGADIVVHSLTKYLGGHGNSVGGAIVDSGKFPWAQHKERFKRL
NEPDVSYHGVVYTDALGPAAYIGRARVVPLRNTGAAISPFNAFLILQGIETLALRMDRICSNALAVAKYLQRHAKVGWVN
YAGLEDHPDHALVQKYLSGKASGLLTFGIKSPAGGGREAGARFLDALGLFTRLVNIGDAKSLATHPASTTHRQLSPEELL
KAGVGEDTVRLSLGIEHIDDLLADLEQALAGV

Specific function: Methionine biosynthesis; second step. [C]

COG id: COG2873

COG function: function code E; O-acetylhomoserine sulfhydrylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the trans-sulfuration enzymes family [H]

Homologues:

Organism=Homo sapiens, GI21361334, Length=411, Percent_Identity=33.5766423357664, Blast_Score=194, Evalue=1e-49,
Organism=Homo sapiens, GI299473759, Length=426, Percent_Identity=31.2206572769953, Blast_Score=154, Evalue=2e-37,
Organism=Homo sapiens, GI25453487, Length=425, Percent_Identity=30.3529411764706, Blast_Score=137, Evalue=2e-32,
Organism=Escherichia coli, GI1790375, Length=423, Percent_Identity=31.6784869976359, Blast_Score=187, Evalue=1e-48,
Organism=Escherichia coli, GI1789383, Length=438, Percent_Identity=26.2557077625571, Blast_Score=125, Evalue=5e-30,
Organism=Caenorhabditis elegans, GI17538055, Length=427, Percent_Identity=34.8946135831382, Blast_Score=200, Evalue=1e-51,
Organism=Caenorhabditis elegans, GI71980708, Length=438, Percent_Identity=29.4520547945205, Blast_Score=195, Evalue=3e-50,
Organism=Caenorhabditis elegans, GI17560056, Length=431, Percent_Identity=34.1067285382831, Blast_Score=191, Evalue=5e-49,
Organism=Saccharomyces cerevisiae, GI6323333, Length=437, Percent_Identity=47.1395881006865, Blast_Score=383, Evalue=1e-107,
Organism=Saccharomyces cerevisiae, GI6319307, Length=430, Percent_Identity=33.953488372093, Blast_Score=191, Evalue=2e-49,
Organism=Saccharomyces cerevisiae, GI6321254, Length=449, Percent_Identity=26.9487750556793, Blast_Score=133, Evalue=5e-32,
Organism=Saccharomyces cerevisiae, GI6321170, Length=339, Percent_Identity=28.023598820059, Blast_Score=113, Evalue=6e-26,
Organism=Saccharomyces cerevisiae, GI6321904, Length=433, Percent_Identity=24.0184757505774, Blast_Score=80, Evalue=5e-16,
Organism=Drosophila melanogaster, GI19922554, Length=430, Percent_Identity=34.6511627906977, Blast_Score=202, Evalue=4e-52,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000277
- InterPro:   IPR006237
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422 [H]

Pfam domain/function: PF01053 Cys_Met_Meta_PP [H]

EC number: =4.4.1.11 [H]

Molecular weight: Translated: 46139; Mature: 46008

Theoretical pI: Translated: 6.84; Mature: 6.84

Prosite motif: PS00868 CYS_MET_METAB_PP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
0.7 %Met     (Translated Protein)
0.9 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
0.5 %Met     (Mature Protein)
0.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSDRPPLKFETLAVHGGYKPDPTTKAVAVPIYQTVAYAFDSAQHGADLFDLKVPGNIYTR
CCCCCCCEEEEEEEECCCCCCCCCCEEEHHHHHHHHHHHHHHCCCCCEEEEECCCHHHHH
IMNPTQDVLEQRVAALEGGIAALALASGQAAVTYAILTIAEAGDNIVASSALYGGTYNLL
CCCCHHHHHHHHHHHHHCCHHEEEECCCCHHHHHHHHHHHHCCCCEEEHHHHCCCHHHHH
AHTLPQYGIATRFADYRDPASFEPLIDGKTKAVFVESLGNPQGNITDIAKIAEVAHRHGV
HHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCEEEEECCCCCCCCHHHHHHHHHHHHHCCC
PLIVDNTVPSPYLSRPFEHGADIVVHSLTKYLGGHGNSVGGAIVDSGKFPWAQHKERFKR
CEEECCCCCCHHHCCCHHCCHHHHHHHHHHHHCCCCCCCCCEEEECCCCCCHHHHHHHHH
LNEPDVSYHGVVYTDALGPAAYIGRARVVPLRNTGAAISPFNAFLILQGIETLALRMDRI
CCCCCCCCCCEEEECCCCHHHHHCCEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHH
CSNALAVAKYLQRHAKVGWVNYAGLEDHPDHALVQKYLSGKASGLLTFGIKSPAGGGREA
HHHHHHHHHHHHHHCCCCEEEECCCCCCCCHHHHHHHHCCCCCCEEEEECCCCCCCCHHH
GARFLDALGLFTRLVNIGDAKSLATHPASTTHRQLSPEELLKAGVGEDTVRLSLGIEHID
HHHHHHHHHHHHHHHCCCCCHHHCCCCCCCCCCCCCHHHHHHCCCCCCEEEEEECHHHHH
DLLADLEQALAGV
HHHHHHHHHHCCC
>Mature Secondary Structure 
SDRPPLKFETLAVHGGYKPDPTTKAVAVPIYQTVAYAFDSAQHGADLFDLKVPGNIYTR
CCCCCCEEEEEEEECCCCCCCCCCEEEHHHHHHHHHHHHHHCCCCCEEEEECCCHHHHH
IMNPTQDVLEQRVAALEGGIAALALASGQAAVTYAILTIAEAGDNIVASSALYGGTYNLL
CCCCHHHHHHHHHHHHHCCHHEEEECCCCHHHHHHHHHHHHCCCCEEEHHHHCCCHHHHH
AHTLPQYGIATRFADYRDPASFEPLIDGKTKAVFVESLGNPQGNITDIAKIAEVAHRHGV
HHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCEEEEECCCCCCCCHHHHHHHHHHHHHCCC
PLIVDNTVPSPYLSRPFEHGADIVVHSLTKYLGGHGNSVGGAIVDSGKFPWAQHKERFKR
CEEECCCCCCHHHCCCHHCCHHHHHHHHHHHHCCCCCCCCCEEEECCCCCCHHHHHHHHH
LNEPDVSYHGVVYTDALGPAAYIGRARVVPLRNTGAAISPFNAFLILQGIETLALRMDRI
CCCCCCCCCCEEEECCCCHHHHHCCEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHH
CSNALAVAKYLQRHAKVGWVNYAGLEDHPDHALVQKYLSGKASGLLTFGIKSPAGGGREA
HHHHHHHHHHHHHHCCCCEEEECCCCCCCCHHHHHHHHCCCCCCEEEEECCCCCCCCHHH
GARFLDALGLFTRLVNIGDAKSLATHPASTTHRQLSPEELLKAGVGEDTVRLSLGIEHID
HHHHHHHHHHHHHHHCCCCCHHHCCCCCCCCCCCCCHHHHHHCCCCCCEEEEEECHHHHH
DLLADLEQALAGV
HHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8586629; 9190812; 3365412 [H]