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The map label for this gene is dnaN [H]

Identifier: 124265195

GI number: 124265195

Start: 1824

End: 2930

Strand: Direct

Name: dnaN [H]

Synonym: Mpe_A0002

Alternate gene names: 124265195

Gene position: 1824-2930 (Clockwise)

Preceding gene: 124265194

Following gene: 124265196

Centisome position: 0.05

GC content: 63.96

Gene sequence:

>1107_bases
ATGATTGTCTTGAAGAGCCCGCAGGAAAAATTTCTGGGTGCACTGCAAGCCGTCGCCGGCATCGTCGAACGGCGTCATAC
CTTGCCGGTGCTGGCCAACGTGCTGATGCGCAAGACCGGCAACGCGATCGAATTCACCACCAGCGATCTCGAGATCCAGG
TCCGCACCCAGGCCGAGTTCGACGGCGACGCCGGCAACTTCAGCACCACGGTCGGAGCGCGCAAGCTGATCGACATCTTG
CGCACCCTGCCGGCCGACCAGAACGTGACGCTCAGCAGCGCGCAGAACAAGCTCACGCTCACCGGCGGCAAGAGTCGCTT
CACGCTGCAGACGCTGCCGGCGGAAGACTTCCCGCTGGTGCAGGAGGCAGCTGATTTCGGCCCGGCCTTCAGCGTGCCGC
AGAAGAGCCTGAAGGCGCTGATCGCCCAGGTGCACTTCGCGATGGCGGTGCACGACATCCGCTACTACCTCAACGGCATC
CTGTTCGTCGCCGAGGGCAAGAGTCTCACGCTGGTGGCCACCGACGGCCACCGCCTGGCGCTGGCCCAGGCCACGCTCGA
TGTGGAGATGCCCAAGCAGGAAGTCATCCTGCCGCGCAAGACCGTGCTGGAGCTGCAGCGCCTGCTGAAGGACGAAGACA
CCGCCATCGAGATGCGCTTCGCCGGCAACCAGGCCAAGTTCGCGTTCTCGGGCATGGAGTTCGTGACCAAGCTGGTCGAG
GGCAAGTTCCCCGACTACAACCGCGTGATCCCCAAGAACCACAAGAACCACGTGACGCTGGGCCGTGCGCCGCTGCTGGC
CAGCCTGCAGCGCGCCGCCATCCTGACCAGCGAGAAGTTCAAGGGCGTGCGCCTGAACTTCGACCCCGGCACGCTGCGCA
TCGCCTCCAGCAACGCCGAGCAGGAAGAGGCCAAGGAAGAGCTCGAGATCGACTACGGCGGCGACGCCATCGAGATCGGC
TTCAACGTCACCTACCTGATGGACGTGCTGGCCAACATGGGCCAAGAGATGGTCACCTTGTCGCTGCAGGATTCCAACAG
CTCGGCTCTCGTCACCGTGCCCGAGCAAGGCGGCTTCAAGTACGTGGTGATGCCGATGCGGATCTAG

Upstream 100 bases:

>100_bases
AGGGTTGAAGCCCAGCTAAGTGATTGCCGGAACAAGCGAAAATGGCCTTGTCCACACGAAGTGCCGGCCCCTACTAAAAC
GACTACCTGAAGAGGTTTAA

Downstream 100 bases:

>100_bases
CCCGCAACCCAGCCGACACCAGCACAGCGGGCCGCGAGCCCGCTGCAGCGTTTTTGAAGGGGCCGGTCCACCGGCCCCGT
GATGGAAAAGAGCCCCCATG

Product: DNA polymerase III subunit beta

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 368; Mature: 368

Protein sequence:

>368_residues
MIVLKSPQEKFLGALQAVAGIVERRHTLPVLANVLMRKTGNAIEFTTSDLEIQVRTQAEFDGDAGNFSTTVGARKLIDIL
RTLPADQNVTLSSAQNKLTLTGGKSRFTLQTLPAEDFPLVQEAADFGPAFSVPQKSLKALIAQVHFAMAVHDIRYYLNGI
LFVAEGKSLTLVATDGHRLALAQATLDVEMPKQEVILPRKTVLELQRLLKDEDTAIEMRFAGNQAKFAFSGMEFVTKLVE
GKFPDYNRVIPKNHKNHVTLGRAPLLASLQRAAILTSEKFKGVRLNFDPGTLRIASSNAEQEEAKEELEIDYGGDAIEIG
FNVTYLMDVLANMGQEMVTLSLQDSNSSALVTVPEQGGFKYVVMPMRI

Sequences:

>Translated_368_residues
MIVLKSPQEKFLGALQAVAGIVERRHTLPVLANVLMRKTGNAIEFTTSDLEIQVRTQAEFDGDAGNFSTTVGARKLIDIL
RTLPADQNVTLSSAQNKLTLTGGKSRFTLQTLPAEDFPLVQEAADFGPAFSVPQKSLKALIAQVHFAMAVHDIRYYLNGI
LFVAEGKSLTLVATDGHRLALAQATLDVEMPKQEVILPRKTVLELQRLLKDEDTAIEMRFAGNQAKFAFSGMEFVTKLVE
GKFPDYNRVIPKNHKNHVTLGRAPLLASLQRAAILTSEKFKGVRLNFDPGTLRIASSNAEQEEAKEELEIDYGGDAIEIG
FNVTYLMDVLANMGQEMVTLSLQDSNSSALVTVPEQGGFKYVVMPMRI
>Mature_368_residues
MIVLKSPQEKFLGALQAVAGIVERRHTLPVLANVLMRKTGNAIEFTTSDLEIQVRTQAEFDGDAGNFSTTVGARKLIDIL
RTLPADQNVTLSSAQNKLTLTGGKSRFTLQTLPAEDFPLVQEAADFGPAFSVPQKSLKALIAQVHFAMAVHDIRYYLNGI
LFVAEGKSLTLVATDGHRLALAQATLDVEMPKQEVILPRKTVLELQRLLKDEDTAIEMRFAGNQAKFAFSGMEFVTKLVE
GKFPDYNRVIPKNHKNHVTLGRAPLLASLQRAAILTSEKFKGVRLNFDPGTLRIASSNAEQEEAKEELEIDYGGDAIEIG
FNVTYLMDVLANMGQEMVTLSLQDSNSSALVTVPEQGGFKYVVMPMRI

Specific function: DNA polymerase III is a complex, multichain enzyme responsible for most of the replicative synthesis in bacteria. This DNA polymerase also exhibits 3' to 5' exonuclease activity. The beta chain is required for initiation of replication once it is clamped

COG id: COG0592

COG function: function code L; DNA polymerase sliding clamp subunit (PCNA homolog)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1790136, Length=361, Percent_Identity=40.9972299168975, Blast_Score=276, Evalue=1e-75,

Paralogues:

None

Copy number: 5954 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001001
- InterPro:   IPR022635
- InterPro:   IPR022637
- InterPro:   IPR022634 [H]

Pfam domain/function: PF00712 DNA_pol3_beta; PF02767 DNA_pol3_beta_2; PF02768 DNA_pol3_beta_3 [H]

EC number: =2.7.7.7 [H]

Molecular weight: Translated: 40450; Mature: 40450

Theoretical pI: Translated: 6.27; Mature: 6.27

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIVLKSPQEKFLGALQAVAGIVERRHTLPVLANVLMRKTGNAIEFTTSDLEIQVRTQAEF
CEEECCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCEEEEEECCEEEEEEEECCC
DGDAGNFSTTVGARKLIDILRTLPADQNVTLSSAQNKLTLTGGKSRFTLQTLPAEDFPLV
CCCCCCCCHHHHHHHHHHHHHHCCCCCCEEEECCCCEEEEECCCCEEEEEECCCCCCCHH
QEAADFGPAFSVPQKSLKALIAQVHFAMAVHDIRYYLNGILFVAEGKSLTLVATDGHRLA
HHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCEEEEEECCCEEEEEEECCCEEE
LAQATLDVEMPKQEVILPRKTVLELQRLLKDEDTAIEMRFAGNQAKFAFSGMEFVTKLVE
EEEEEEEECCCHHHCCCCHHHHHHHHHHHCCCCCEEEEEECCCCCEEEECHHHHHHHHHC
GKFPDYNRVIPKNHKNHVTLGRAPLLASLQRAAILTSEKFKGVRLNFDPGTLRIASSNAE
CCCCCCCCCCCCCCCCCEEECCCHHHHHHHHHHHHHHCCCCCEEEEECCCEEEEECCCCH
QEEAKEELEIDYGGDAIEIGFNVTYLMDVLANMGQEMVTLSLQDSNSSALVTVPEQGGFK
HHHHHHHEEEECCCCEEEECCCHHHHHHHHHHCCCEEEEEEEECCCCCEEEEECCCCCEE
YVVMPMRI
EEEEEECC
>Mature Secondary Structure
MIVLKSPQEKFLGALQAVAGIVERRHTLPVLANVLMRKTGNAIEFTTSDLEIQVRTQAEF
CEEECCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCEEEEEECCEEEEEEEECCC
DGDAGNFSTTVGARKLIDILRTLPADQNVTLSSAQNKLTLTGGKSRFTLQTLPAEDFPLV
CCCCCCCCHHHHHHHHHHHHHHCCCCCCEEEECCCCEEEEECCCCEEEEEECCCCCCCHH
QEAADFGPAFSVPQKSLKALIAQVHFAMAVHDIRYYLNGILFVAEGKSLTLVATDGHRLA
HHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCEEEEEECCCEEEEEEECCCEEE
LAQATLDVEMPKQEVILPRKTVLELQRLLKDEDTAIEMRFAGNQAKFAFSGMEFVTKLVE
EEEEEEEECCCHHHCCCCHHHHHHHHHHHCCCCCEEEEEECCCCCEEEECHHHHHHHHHC
GKFPDYNRVIPKNHKNHVTLGRAPLLASLQRAAILTSEKFKGVRLNFDPGTLRIASSNAE
CCCCCCCCCCCCCCCCCEEECCCHHHHHHHHHHHHHHCCCCCEEEEECCCEEEEECCCCH
QEEAKEELEIDYGGDAIEIGFNVTYLMDVLANMGQEMVTLSLQDSNSSALVTVPEQGGFK
HHHHHHHEEEECCCCEEEECCCHHHHHHHHHHCCCEEEEEEEECCCCCEEEEECCCCCEE
YVVMPMRI
EEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10984043 [H]