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The map label for this gene is gyrB [H]

Identifier: 124265196

GI number: 124265196

Start: 3028

End: 5583

Strand: Direct

Name: gyrB [H]

Synonym: Mpe_A0003

Alternate gene names: 124265196

Gene position: 3028-5583 (Clockwise)

Preceding gene: 124265195

Following gene: 124265197

Centisome position: 0.07

GC content: 66.04

Gene sequence:

>2556_bases
ATGAGCGACCCCAAGACCCCGCAACACGACGCCCAGAGCGGCGTCACGCAGCAACCCGTGCCGTCGGCGGAAGGCCATGC
CATCTCCGTGGGTTCGGACGCGTCGGTGGCCTATGGAGAGGGCTCCATCCAGATCCTGGAAGGCCTGGAGGCGGTTCGCA
AGCGGCCCGGCATGTACATCGGCGACACGTCCGACGGCACCGGCCTGCACCACCTCGTGTTCGAGGTGGTCGACAACTCC
ATCGACGAAGCCCTGGCCGGCCACTGCGACGACATCGTGGTGACCATCCACTCCGACAACTCCATCAGCGTCACCGACAA
CGGCCGCGGCATCCCCACCGGCGTGAAGATGGACGACAAGCACGAGCCCAAGCGCAGTGCCGCGGAGATCGCGCTGACCG
AGCTGCACGCTGGCGGCAAGTTCAACCAGAACAGCTACAAGGTCTCGGGCGGGCTGCACGGCGTGGGTGTGAGTTGCGTC
AACGCGCTCTCGAAGTGGTTGCGCCTCACCGTACGCCGCGAAGGCAAGGTGCACCTCATGGAGTTCCGGCGCGGATTCCC
GCAGGACCGTGTGCTGGAGATGCGCAACGGCTTCGAGGTCTCGCCGATGGCCATCGTGGGCGACACCGAGAAGCGCGGCA
CCGAAGTGCACTTCCTGCCCGACGAAGAGATCTTCACCAACATCGACTTCCACTACGACGTGCTCGCCAAGCGCCTGCGC
GAACTGAGCTTCCTGAACAACGGCGTGAAGATCCGCCTGGTCGACGAGCGCAACGCCAAGGAAGACAACTTCGCCTACGC
CGGCGGCGTGAAGGGGTTCGTGGAGTTCATCAACACCGGCAAGAAGGTGCTGCACGGCAACATCTTCCACGCGCTGGGCG
ACAAGATGAGCGAGTTCAACACCAACATCGGTGTCGAAGTGGCGATGCAGTGGAACGACGGCTACAGCGAACAGGTGCTG
TGCTTCACCAACAACATCCCGCAGCGCGACGGCGGTACCCACCTCACCGGCCTGCGCGCGGCCATGACGCGCGTGATCAA
CAAGTACATCGAGGACAACGAACTCGCCAAGAAGGCCAAGGTCGAGATCGCCGGCGACGACATGCGCGAAGGGCTGGCCT
GCGTGGTGAGCGTGAAGGTGCCCGAGCCCAAGTTCAGCAGCCAGACCAAGGACAAGCTGGTGTCCAGCGAAGTGCGCGGC
CCGGTGGAAGAGATCGTGGGCAAGCTGCTCACCGACTACCTGCTCGAGAACCCGATCGACGCCAAGATCATCTGCGGCAA
GATCGTGGAGGCCGCGCGCGCCCGCGAGGCCGCCCGCAAGGCCCGCGAGATGACGCGCCGCAAGGGCGTACTCGACGGCA
TGGGCCTGCCCGGCAAGCTGGCCGACTGCCAGGAGAAGGACCCCGCGCTCTGCGAGATCTACATCGTGGAGGGCGACTCC
GCCGGCGGCTCCGCCAAGCAGGGCCGCGACCGCAAGTTCCAGGCGATCCTGCCGCTGCGCGGCAAGATCCTGAACGTGGA
GCGCGCCCGCTTCGAAAAGCTGCTCACCAGCAACGAGATCCTCACGCTCATCACCGCGCTGGGCACCAGCATCGGCAAGG
ACGAATACAACCCCGACAAGCTGCGCTACCACCGCATCATCATCATGACCGATGCCGACGTGGACGGCGCCCACATCCGC
ACGCTGCTGCTCACCTTCTTCTACCGCCAGATGCCCGAGCTGGTGGAGCGCGGCCACATCTACATCGCCCAGCCGCCGCT
CTACAAGGTGAAGCTCGGCAAGGAAGAGCAGTACCTGAAGGACGGCCACGAGCTCGACGCCTACCTGTTGCGCGTGGCGC
TGCGCGACGCGCGGCTGGAAACCGGCGTGGGCGGCACCGTGCTGCTGGGCGACGCGCTGGAATCGCTGGCGCGCCAGTAC
GTGCTGGCCAACAACGTGACCGCCCGCCTGGCCAACTGGATGGACGTGGAGGCGCTGCGCGCCATCGCCGACGGCCTGGT
ACTCAACCTCGACACCCTGGCCGATGCCGAAACCTCCGCCGCCGCGCTGAAGGCCGCGCTGCACGATGCCGACGTGAGCG
CCGAATTCGACGCCCGGCTCGACAAGCACCTGCTCCGCATCAGCCGCCGCCACCACGGCAACACCAAGAGCAGCGTGATC
ACCGCCGACTTCGTGCACGGCGCCGACTACGAGGCCCTCAGCACCGCCGGCCTCGCCTTCAAGGGCCTGGTGTCCACCGA
GGCCGTGGTGAAGAAGGGCGAGGGCGAGAAGCAGAAGGACGCCAAGGTGGCCGACTTCCGCGAGGCCATGGCCTGGCTGA
TGCAGCAGGCCGAGAACAGCGTGGGCCGCCAGCGCTACAAGGGCCTGGGCGAGATGAACCCCGAGCAGCTCTGGGAAACC
ACCATGGACCCCACGGTGCGCCGCCTGCTGCGCGTACAGATCGACGACGCCATCGAGGCCGACCGCGTGTTCACCATGCT
GATGGGCGACGAGGTGGAACCGCGGCGCGACTTCATCGAGACGAATGCGTTGAGGGCGGCGAACATCGACGTTTGA

Upstream 100 bases:

>100_bases
TAGCCCGCAACCCAGCCGACACCAGCACAGCGGGCCGCGAGCCCGCTGCAGCGTTTTTGAAGGGGCCGGTCCACCGGCCC
CGTGATGGAAAAGAGCCCCC

Downstream 100 bases:

>100_bases
TCCGGGAGCCGGATTGAACGAGATGTACGAAATCAACCAGAACGAAGTCCCCTCTTCGTTCATCGCTCTGTTCATCCCCC
GCGGCGCCACGCGCCCCAGC

Product: DNA gyrase subunit B

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 851; Mature: 850

Protein sequence:

>851_residues
MSDPKTPQHDAQSGVTQQPVPSAEGHAISVGSDASVAYGEGSIQILEGLEAVRKRPGMYIGDTSDGTGLHHLVFEVVDNS
IDEALAGHCDDIVVTIHSDNSISVTDNGRGIPTGVKMDDKHEPKRSAAEIALTELHAGGKFNQNSYKVSGGLHGVGVSCV
NALSKWLRLTVRREGKVHLMEFRRGFPQDRVLEMRNGFEVSPMAIVGDTEKRGTEVHFLPDEEIFTNIDFHYDVLAKRLR
ELSFLNNGVKIRLVDERNAKEDNFAYAGGVKGFVEFINTGKKVLHGNIFHALGDKMSEFNTNIGVEVAMQWNDGYSEQVL
CFTNNIPQRDGGTHLTGLRAAMTRVINKYIEDNELAKKAKVEIAGDDMREGLACVVSVKVPEPKFSSQTKDKLVSSEVRG
PVEEIVGKLLTDYLLENPIDAKIICGKIVEAARAREAARKAREMTRRKGVLDGMGLPGKLADCQEKDPALCEIYIVEGDS
AGGSAKQGRDRKFQAILPLRGKILNVERARFEKLLTSNEILTLITALGTSIGKDEYNPDKLRYHRIIIMTDADVDGAHIR
TLLLTFFYRQMPELVERGHIYIAQPPLYKVKLGKEEQYLKDGHELDAYLLRVALRDARLETGVGGTVLLGDALESLARQY
VLANNVTARLANWMDVEALRAIADGLVLNLDTLADAETSAAALKAALHDADVSAEFDARLDKHLLRISRRHHGNTKSSVI
TADFVHGADYEALSTAGLAFKGLVSTEAVVKKGEGEKQKDAKVADFREAMAWLMQQAENSVGRQRYKGLGEMNPEQLWET
TMDPTVRRLLRVQIDDAIEADRVFTMLMGDEVEPRRDFIETNALRAANIDV

Sequences:

>Translated_851_residues
MSDPKTPQHDAQSGVTQQPVPSAEGHAISVGSDASVAYGEGSIQILEGLEAVRKRPGMYIGDTSDGTGLHHLVFEVVDNS
IDEALAGHCDDIVVTIHSDNSISVTDNGRGIPTGVKMDDKHEPKRSAAEIALTELHAGGKFNQNSYKVSGGLHGVGVSCV
NALSKWLRLTVRREGKVHLMEFRRGFPQDRVLEMRNGFEVSPMAIVGDTEKRGTEVHFLPDEEIFTNIDFHYDVLAKRLR
ELSFLNNGVKIRLVDERNAKEDNFAYAGGVKGFVEFINTGKKVLHGNIFHALGDKMSEFNTNIGVEVAMQWNDGYSEQVL
CFTNNIPQRDGGTHLTGLRAAMTRVINKYIEDNELAKKAKVEIAGDDMREGLACVVSVKVPEPKFSSQTKDKLVSSEVRG
PVEEIVGKLLTDYLLENPIDAKIICGKIVEAARAREAARKAREMTRRKGVLDGMGLPGKLADCQEKDPALCEIYIVEGDS
AGGSAKQGRDRKFQAILPLRGKILNVERARFEKLLTSNEILTLITALGTSIGKDEYNPDKLRYHRIIIMTDADVDGAHIR
TLLLTFFYRQMPELVERGHIYIAQPPLYKVKLGKEEQYLKDGHELDAYLLRVALRDARLETGVGGTVLLGDALESLARQY
VLANNVTARLANWMDVEALRAIADGLVLNLDTLADAETSAAALKAALHDADVSAEFDARLDKHLLRISRRHHGNTKSSVI
TADFVHGADYEALSTAGLAFKGLVSTEAVVKKGEGEKQKDAKVADFREAMAWLMQQAENSVGRQRYKGLGEMNPEQLWET
TMDPTVRRLLRVQIDDAIEADRVFTMLMGDEVEPRRDFIETNALRAANIDV
>Mature_850_residues
SDPKTPQHDAQSGVTQQPVPSAEGHAISVGSDASVAYGEGSIQILEGLEAVRKRPGMYIGDTSDGTGLHHLVFEVVDNSI
DEALAGHCDDIVVTIHSDNSISVTDNGRGIPTGVKMDDKHEPKRSAAEIALTELHAGGKFNQNSYKVSGGLHGVGVSCVN
ALSKWLRLTVRREGKVHLMEFRRGFPQDRVLEMRNGFEVSPMAIVGDTEKRGTEVHFLPDEEIFTNIDFHYDVLAKRLRE
LSFLNNGVKIRLVDERNAKEDNFAYAGGVKGFVEFINTGKKVLHGNIFHALGDKMSEFNTNIGVEVAMQWNDGYSEQVLC
FTNNIPQRDGGTHLTGLRAAMTRVINKYIEDNELAKKAKVEIAGDDMREGLACVVSVKVPEPKFSSQTKDKLVSSEVRGP
VEEIVGKLLTDYLLENPIDAKIICGKIVEAARAREAARKAREMTRRKGVLDGMGLPGKLADCQEKDPALCEIYIVEGDSA
GGSAKQGRDRKFQAILPLRGKILNVERARFEKLLTSNEILTLITALGTSIGKDEYNPDKLRYHRIIIMTDADVDGAHIRT
LLLTFFYRQMPELVERGHIYIAQPPLYKVKLGKEEQYLKDGHELDAYLLRVALRDARLETGVGGTVLLGDALESLARQYV
LANNVTARLANWMDVEALRAIADGLVLNLDTLADAETSAAALKAALHDADVSAEFDARLDKHLLRISRRHHGNTKSSVIT
ADFVHGADYEALSTAGLAFKGLVSTEAVVKKGEGEKQKDAKVADFREAMAWLMQQAENSVGRQRYKGLGEMNPEQLWETT
MDPTVRRLLRVQIDDAIEADRVFTMLMGDEVEPRRDFIETNALRAANIDV

Specific function: DNA gyrase negatively supercoils closed circular double- stranded DNA in an ATP-dependent manner and also catalyzes the interconversion of other topological isomers of double-stranded DNA rings, including catenanes and knotted rings [H]

COG id: COG0187

COG function: function code L; Type IIA topoisomerase (DNA gyrase/topo II, topoisomerase IV), B subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the type II topoisomerase family [H]

Homologues:

Organism=Homo sapiens, GI19913406, Length=572, Percent_Identity=25.5244755244755, Blast_Score=123, Evalue=6e-28,
Organism=Homo sapiens, GI19913408, Length=582, Percent_Identity=25.7731958762887, Blast_Score=119, Evalue=1e-26,
Organism=Escherichia coli, GI48994957, Length=835, Percent_Identity=59.0419161676647, Blast_Score=949, Evalue=0.0,
Organism=Escherichia coli, GI1789408, Length=564, Percent_Identity=39.0070921985816, Blast_Score=369, Evalue=1e-103,
Organism=Caenorhabditis elegans, GI17535065, Length=593, Percent_Identity=23.9460370994941, Blast_Score=124, Evalue=2e-28,
Organism=Caenorhabditis elegans, GI212645845, Length=530, Percent_Identity=24.7169811320755, Blast_Score=103, Evalue=5e-22,
Organism=Caenorhabditis elegans, GI212645657, Length=153, Percent_Identity=33.9869281045752, Blast_Score=74, Evalue=4e-13,
Organism=Saccharomyces cerevisiae, GI6324241, Length=602, Percent_Identity=24.9169435215947, Blast_Score=109, Evalue=1e-24,
Organism=Drosophila melanogaster, GI17136538, Length=548, Percent_Identity=25.5474452554745, Blast_Score=116, Evalue=8e-26,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR011557
- InterPro:   IPR020568
- InterPro:   IPR014721
- InterPro:   IPR001241
- InterPro:   IPR013759
- InterPro:   IPR002288
- InterPro:   IPR013506
- InterPro:   IPR013760
- InterPro:   IPR018522 [H]

Pfam domain/function: PF00204 DNA_gyraseB; PF00986 DNA_gyraseB_C; PF02518 HATPase_c [H]

EC number: =5.99.1.3 [H]

Molecular weight: Translated: 93915; Mature: 93784

Theoretical pI: Translated: 6.04; Mature: 6.04

Prosite motif: PS00177 TOPOISOMERASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSDPKTPQHDAQSGVTQQPVPSAEGHAISVGSDASVAYGEGSIQILEGLEAVRKRPGMYI
CCCCCCCCCHHHCCCCCCCCCCCCCCEEEECCCCCEEECCCCHHHHHHHHHHHHCCCEEE
GDTSDGTGLHHLVFEVVDNSIDEALAGHCDDIVVTIHSDNSISVTDNGRGIPTGVKMDDK
ECCCCCCHHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCEEEECCCCCCCCCCCCCCCC
HEPKRSAAEIALTELHAGGKFNQNSYKVSGGLHGVGVSCVNALSKWLRLTVRREGKVHLM
CCCHHHHHHHHHHHHCCCCCCCCCCEEECCCCCCHHHHHHHHHHHHHHHHHHCCCCEEHH
EFRRGFPQDRVLEMRNGFEVSPMAIVGDTEKRGTEVHFLPDEEIFTNIDFHYDVLAKRLR
HHHCCCCHHHHHHHHCCCCCCCEEEEECCCCCCCEEEECCCHHHHCCCCCHHHHHHHHHH
ELSFLNNGVKIRLVDERNAKEDNFAYAGGVKGFVEFINTGKKVLHGNIFHALGDKMSEFN
HHHHHCCCCEEEEEECCCCCCCCCEECCCHHHHHHHHHCCHHHHHCHHHHHHHHHHHHHC
TNIGVEVAMQWNDGYSEQVLCFTNNIPQRDGGTHLTGLRAAMTRVINKYIEDNELAKKAK
CCCCEEEEEEECCCCCCEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHCCHHHHHHHE
VEIAGDDMREGLACVVSVKVPEPKFSSQTKDKLVSSEVRGPVEEIVGKLLTDYLLENPID
EEECCCHHHCCEEEEEEEECCCCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCC
AKIICGKIVEAARAREAARKAREMTRRKGVLDGMGLPGKLADCQEKDPALCEIYIVEGDS
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCEEEEEEEECCC
AGGSAKQGRDRKFQAILPLRGKILNVERARFEKLLTSNEILTLITALGTSIGKDEYNPDK
CCCCCCCCCCCCEEEECCCCCEEECHHHHHHHHHHCCCHHHHHHHHHCCCCCCCCCCCCC
LRYHRIIIMTDADVDGAHIRTLLLTFFYRQMPELVERGHIYIAQPPLYKVKLGKEEQYLK
EEEEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHCCCEEEECCCEEEEECCCCHHHHH
DGHELDAYLLRVALRDARLETGVGGTVLLGDALESLARQYVLANNVTARLANWMDVEALR
CCHHHHHHHHHHHHHHHHHHCCCCCEEEHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHH
AIADGLVLNLDTLADAETSAAALKAALHDADVSAEFDARLDKHLLRISRRHHGNTKSSVI
HHHCCCEEEEHHHCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCCCCCCEE
TADFVHGADYEALSTAGLAFKGLVSTEAVVKKGEGEKQKDAKVADFREAMAWLMQQAENS
EEEEECCCCCHHHHHCCHHHHHHHHHHHHHHCCCCCCHHCCHHHHHHHHHHHHHHHHHHH
VGRQRYKGLGEMNPEQLWETTMDPTVRRLLRVQIDDAIEADRVFTMLMGDEVEPRRDFIE
HHHHHHHCCCCCCHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHH
TNALRAANIDV
HCCCCCCCCCC
>Mature Secondary Structure 
SDPKTPQHDAQSGVTQQPVPSAEGHAISVGSDASVAYGEGSIQILEGLEAVRKRPGMYI
CCCCCCCCHHHCCCCCCCCCCCCCCEEEECCCCCEEECCCCHHHHHHHHHHHHCCCEEE
GDTSDGTGLHHLVFEVVDNSIDEALAGHCDDIVVTIHSDNSISVTDNGRGIPTGVKMDDK
ECCCCCCHHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCEEEECCCCCCCCCCCCCCCC
HEPKRSAAEIALTELHAGGKFNQNSYKVSGGLHGVGVSCVNALSKWLRLTVRREGKVHLM
CCCHHHHHHHHHHHHCCCCCCCCCCEEECCCCCCHHHHHHHHHHHHHHHHHHCCCCEEHH
EFRRGFPQDRVLEMRNGFEVSPMAIVGDTEKRGTEVHFLPDEEIFTNIDFHYDVLAKRLR
HHHCCCCHHHHHHHHCCCCCCCEEEEECCCCCCCEEEECCCHHHHCCCCCHHHHHHHHHH
ELSFLNNGVKIRLVDERNAKEDNFAYAGGVKGFVEFINTGKKVLHGNIFHALGDKMSEFN
HHHHHCCCCEEEEEECCCCCCCCCEECCCHHHHHHHHHCCHHHHHCHHHHHHHHHHHHHC
TNIGVEVAMQWNDGYSEQVLCFTNNIPQRDGGTHLTGLRAAMTRVINKYIEDNELAKKAK
CCCCEEEEEEECCCCCCEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHCCHHHHHHHE
VEIAGDDMREGLACVVSVKVPEPKFSSQTKDKLVSSEVRGPVEEIVGKLLTDYLLENPID
EEECCCHHHCCEEEEEEEECCCCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCC
AKIICGKIVEAARAREAARKAREMTRRKGVLDGMGLPGKLADCQEKDPALCEIYIVEGDS
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCEEEEEEEECCC
AGGSAKQGRDRKFQAILPLRGKILNVERARFEKLLTSNEILTLITALGTSIGKDEYNPDK
CCCCCCCCCCCCEEEECCCCCEEECHHHHHHHHHHCCCHHHHHHHHHCCCCCCCCCCCCC
LRYHRIIIMTDADVDGAHIRTLLLTFFYRQMPELVERGHIYIAQPPLYKVKLGKEEQYLK
EEEEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHCCCEEEECCCEEEEECCCCHHHHH
DGHELDAYLLRVALRDARLETGVGGTVLLGDALESLARQYVLANNVTARLANWMDVEALR
CCHHHHHHHHHHHHHHHHHHCCCCCEEEHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHH
AIADGLVLNLDTLADAETSAAALKAALHDADVSAEFDARLDKHLLRISRRHHGNTKSSVI
HHHCCCEEEEHHHCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCCCCCCEE
TADFVHGADYEALSTAGLAFKGLVSTEAVVKKGEGEKQKDAKVADFREAMAWLMQQAENS
EEEEECCCCCHHHHHCCHHHHHHHHHHHHHHCCCCCCHHCCHHHHHHHHHHHHHHHHHHH
VGRQRYKGLGEMNPEQLWETTMDPTVRRLLRVQIDDAIEADRVFTMLMGDEVEPRRDFIE
HHHHHHHCCCCCCHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHH
TNALRAANIDV
HCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]