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The map label for this gene is hemC

Identifier: 124027712

GI number: 124027712

Start: 832447

End: 833358

Strand: Reverse

Name: hemC

Synonym: Hbut_0836

Alternate gene names: 124027712

Gene position: 833358-832447 (Counterclockwise)

Preceding gene: 124027713

Following gene: 124027711

Centisome position: 49.99

GC content: 54.93

Gene sequence:

>912_bases
ATGAAGCTCCGCGTAGCTACGCGGGGTAGTAAGCTCAGCATAGCACAGACAATGATAGCTCTGGAGGCAATAAAACGTGT
CGAGCCAAGCCTAGAGTATGAACTAGTGATAGTCAAGACTAGAGGTGATATACACCAGGACAAACCATTTACAGCTATAG
GCGGCAAGGGCCTCTTCGAGAAAGAGGTAAACCTTGCAGTACTGGAGGGACGTGCTGACATAGCAGTTCACAGTCTAAAG
GATGTACCTAGCGCTATTTCGCCCGGTCTAGTACTAGCCATGACGCCGCCGAGGGATCCACCCTACGACGTGCTCGTTGT
GAGAGGCGGGAAGGAGAAGACGATATGGGATCTGCCCAGCGGCGCTATCGTTGGAACATCGAGCGCCCGTAGGGTTGCAA
TGCTCAAACATGTCCGCCGCGACCTAGTCTTCAAGGTTTTAAGGGGTAATGTGGATACCAGGCTTCGAAAGCTAGAGCAA
GGACAGTATGACGCCATAGTGCTTGCCGAGGCCGGGCTAAAGAGGCTAGGTGTAGACATAGAGTATTGGCGTATACCTCC
AGACATCCTGCCCCCAGCGCCTGGCCAGGGCATAATAGGGGTCTATACTCTGTCCAGTCGCAGCGACATACTCCCGATCC
TCGAGAAGGCCTCAGACCAGAAGGCTATGGCCGAGGCTAGGGCTGAGAGAGCATTCCTAGCCTATGCTGGCGGTGGCTGC
CACACCCCACTCGGAGCCTACGCTGAGCTGCGTGGCAACACCCTCTACTTCCATGCAGCATTAGCATCTCCCGATGGCAG
CCGAAGGGTTGAAGTAAGGGTCGAGGGTGATCCGGATAAGCCTACACAGGTAGGCCTAGAGGCAGCATTCGAGCTGAGGA
GGCTAGCAGCAAGGGAGGGGATAAGCCTGTAA

Upstream 100 bases:

>100_bases
GCCAGATGGAGGCAATATTCACGAGTGCAGCCCACACGAGCGACGTAGTCTCCGAGACTATCGAGAAGCTTAGGAAGGCC
TTTAAGAGGCTTAGATAACC

Downstream 100 bases:

>100_bases
TGCAATGCAGTGGTAGAGTCTACATCGTTGGTGCAGGTCCTGGAGACCCTGAGCTGATAACCGTCAAGGGGCTAAAGCTG
ATCCGGGAAGCAGACGTCAT

Product: porphobilinogen deaminase

Products: NA

Alternate protein names: PBG; Hydroxymethylbilane synthase; HMBS; Pre-uroporphyrinogen synthase

Number of amino acids: Translated: 303; Mature: 303

Protein sequence:

>303_residues
MKLRVATRGSKLSIAQTMIALEAIKRVEPSLEYELVIVKTRGDIHQDKPFTAIGGKGLFEKEVNLAVLEGRADIAVHSLK
DVPSAISPGLVLAMTPPRDPPYDVLVVRGGKEKTIWDLPSGAIVGTSSARRVAMLKHVRRDLVFKVLRGNVDTRLRKLEQ
GQYDAIVLAEAGLKRLGVDIEYWRIPPDILPPAPGQGIIGVYTLSSRSDILPILEKASDQKAMAEARAERAFLAYAGGGC
HTPLGAYAELRGNTLYFHAALASPDGSRRVEVRVEGDPDKPTQVGLEAAFELRRLAAREGISL

Sequences:

>Translated_303_residues
MKLRVATRGSKLSIAQTMIALEAIKRVEPSLEYELVIVKTRGDIHQDKPFTAIGGKGLFEKEVNLAVLEGRADIAVHSLK
DVPSAISPGLVLAMTPPRDPPYDVLVVRGGKEKTIWDLPSGAIVGTSSARRVAMLKHVRRDLVFKVLRGNVDTRLRKLEQ
GQYDAIVLAEAGLKRLGVDIEYWRIPPDILPPAPGQGIIGVYTLSSRSDILPILEKASDQKAMAEARAERAFLAYAGGGC
HTPLGAYAELRGNTLYFHAALASPDGSRRVEVRVEGDPDKPTQVGLEAAFELRRLAAREGISL
>Mature_303_residues
MKLRVATRGSKLSIAQTMIALEAIKRVEPSLEYELVIVKTRGDIHQDKPFTAIGGKGLFEKEVNLAVLEGRADIAVHSLK
DVPSAISPGLVLAMTPPRDPPYDVLVVRGGKEKTIWDLPSGAIVGTSSARRVAMLKHVRRDLVFKVLRGNVDTRLRKLEQ
GQYDAIVLAEAGLKRLGVDIEYWRIPPDILPPAPGQGIIGVYTLSSRSDILPILEKASDQKAMAEARAERAFLAYAGGGC
HTPLGAYAELRGNTLYFHAALASPDGSRRVEVRVEGDPDKPTQVGLEAAFELRRLAAREGISL

Specific function: Tetrapolymerization of the monopyrrole PBG into the hydroxymethylbilane pre-uroporphyrinogen in several discrete steps

COG id: COG0181

COG function: function code H; Porphobilinogen deaminase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the HMBS family

Homologues:

Organism=Homo sapiens, GI20149500, Length=318, Percent_Identity=36.1635220125786, Blast_Score=172, Evalue=5e-43,
Organism=Homo sapiens, GI66933009, Length=318, Percent_Identity=36.1635220125786, Blast_Score=171, Evalue=6e-43,
Organism=Escherichia coli, GI48994974, Length=298, Percent_Identity=42.6174496644295, Blast_Score=212, Evalue=3e-56,
Organism=Saccharomyces cerevisiae, GI6319996, Length=283, Percent_Identity=34.2756183745583, Blast_Score=146, Evalue=5e-36,
Organism=Drosophila melanogaster, GI20130425, Length=258, Percent_Identity=34.1085271317829, Blast_Score=142, Evalue=3e-34,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): HEM3_HYPBU (A2BL26)

Other databases:

- EMBL:   CP000493
- RefSeq:   YP_001013032.1
- ProteinModelPortal:   A2BL26
- SMR:   A2BL26
- STRING:   A2BL26
- GeneID:   4781918
- GenomeReviews:   CP000493_GR
- KEGG:   hbu:Hbut_0836
- eggNOG:   arNOG08614
- HOGENOM:   HBG624842
- OMA:   SLHAVGQ
- PhylomeDB:   A2BL26
- ProtClustDB:   PRK00072
- BioCyc:   HBUT415426:HBUT_0836-MONOMER
- HAMAP:   MF_00260
- InterPro:   IPR000860
- InterPro:   IPR022419
- InterPro:   IPR022417
- InterPro:   IPR022418
- Gene3D:   G3DSA:3.30.160.40
- PANTHER:   PTHR11557
- PIRSF:   PIRSF001438
- PRINTS:   PR00151
- TIGRFAMs:   TIGR00212

Pfam domain/function: PF01379 Porphobil_deam; PF03900 Porphobil_deamC; SSF54782 Porphobil_deam

EC number: =2.5.1.61

Molecular weight: Translated: 32927; Mature: 32927

Theoretical pI: Translated: 9.78; Mature: 9.78

Prosite motif: PS00533 PORPHOBILINOGEN_DEAM

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLRVATRGSKLSIAQTMIALEAIKRVEPSLEYELVIVKTRGDIHQDKPFTAIGGKGLFE
CEEEEECCCCCHHHHHHHHHHHHHHHCCCCCCEEEEEEEECCCCCCCCCCEEECCCCCEE
KEVNLAVLEGRADIAVHSLKDVPSAISPGLVLAMTPPRDPPYDVLVVRGGKEKTIWDLPS
CCCCEEEEECCCCEEHHHHHHCCHHCCCCEEEEECCCCCCCCEEEEEECCCCCEEEECCC
GAIVGTSSARRVAMLKHVRRDLVFKVLRGNVDTRLRKLEQGQYDAIVLAEAGLKRLGVDI
CCEEECCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHCCCCCEEEEECCCHHHHCCEE
EYWRIPPDILPPAPGQGIIGVYTLSSRSDILPILEKASDQKAMAEARAERAFLAYAGGGC
EEEECCCCCCCCCCCCCEEEEEEECCCCCHHHHHHCCCCHHHHHHHHHHEEEEEEECCCC
HTPLGAYAELRGNTLYFHAALASPDGSRRVEVRVEGDPDKPTQVGLEAAFELRRLAAREG
CCCCCCHHHHCCCEEEEEEEEECCCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHCC
ISL
CCC
>Mature Secondary Structure
MKLRVATRGSKLSIAQTMIALEAIKRVEPSLEYELVIVKTRGDIHQDKPFTAIGGKGLFE
CEEEEECCCCCHHHHHHHHHHHHHHHCCCCCCEEEEEEEECCCCCCCCCCEEECCCCCEE
KEVNLAVLEGRADIAVHSLKDVPSAISPGLVLAMTPPRDPPYDVLVVRGGKEKTIWDLPS
CCCCEEEEECCCCEEHHHHHHCCHHCCCCEEEEECCCCCCCCEEEEEECCCCCEEEECCC
GAIVGTSSARRVAMLKHVRRDLVFKVLRGNVDTRLRKLEQGQYDAIVLAEAGLKRLGVDI
CCEEECCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHCCCCCEEEEECCCHHHHCCEE
EYWRIPPDILPPAPGQGIIGVYTLSSRSDILPILEKASDQKAMAEARAERAFLAYAGGGC
EEEECCCCCCCCCCCCCEEEEEEECCCCCHHHHHHCCCCHHHHHHHHHHEEEEEEECCCC
HTPLGAYAELRGNTLYFHAALASPDGSRRVEVRVEGDPDKPTQVGLEAAFELRRLAAREG
CCCCCCHHHHCCCEEEEEEEEECCCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHCC
ISL
CCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA