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The map label for this gene is hemL

Identifier: 124027713

GI number: 124027713

Start: 833361

End: 834641

Strand: Reverse

Name: hemL

Synonym: Hbut_0837

Alternate gene names: 124027713

Gene position: 834641-833361 (Counterclockwise)

Preceding gene: 124027714

Following gene: 124027712

Centisome position: 50.06

GC content: 51.6

Gene sequence:

>1281_bases
TTGCCTGGCGAGGTGAGCCGCTCTCTCTACGAGAAGGCACTCACACTATTTCCTGGTGGCGTCAACAGCCCTGTTCGTGC
AGCGGTTAAGCCCTACCCATTTTATGTTGAGCGGGCTGAGGGCCCATACATCTACACTGTTGATGGCGAGAAACTTATAG
ACTACGTGCTGGCCTATGGCCCCCTAATCCTCGGTCATAAACATCCCCGTGTGGAGGAGGCTGTTAGGAGGCAGCTGGAG
AAGGGCTGGCTCTACGGCGCTCCCTACGAGCTGGAGATAAGACTTGCAGAAAAGATACTCAAGTATTACCATCCCGGTGG
AATGGTTCGCTTTGTAAACACGGGCACAGAAGCAACAATGACTGCGATAAGGCTCGCAAGAGGTGTCACTGGCAGGAAGT
ACATTGTAAAGTTTAATGGATGCTACCATGGCGCCCATGATGCTGTGTTGGTGGGAGCTGGTAGTGCAGCAGCAGAGTAT
GGAGTACCGACAAGCAAGGGTATACCCGAGGAAGTTGCCAAGCTAACACTCGTAGCAAAATACAATGACATTGAAAGCGT
AGAGAAGATAATGTCAAAGCATGGTGACGAGGTGGCAGCAATAATAGTCGAACCAGTTGCAGGTAATGCAGGAGTCATAC
CGCCAAAGAAGGGGTTCCTCCAGGAGCTACGCCGCATAGCAAGCGAGCATGGAGCACTGCTAATAATGGACGAGGTTATC
ACTGGGTTCCGCTTAGCCTTGGGCGGCGCCCAGGAATACTATAGAGTAAAGGCAGACATAACCACGCTGGGCAAGATAGT
AGGTGGCGGCTTCCCAATCGGCGTAGTTGTGGCTGACAGGAAGATAATGGAGCATCTGACACCTAGCGGCAAAGTCTTCA
ATGCTGGAACATTTAACGCGCATCCAGTAACAATGGCTGCAGGGCTGGCTACTATTGAAGTTCTCGAGGAGGGCACACCA
TACCGGGTAGCTTCTGAGGCGGGCAGAGCGCTAGCAGAAGAGCTAGAATCGCTCGTATTAAGCTATGGTATAGACGCTGC
TGTAAACCATGTGGAGAGCATGCTGCAGATATTCTTCGTAAAGGGTGAGGTTTGGAGCCCCGAGGACGCAGCAAAGAGCG
ATAAGAAGCTCTACCTGAAGCTACACGAAGAGCTGCTAAAACTAGGCGTATTCATAGCGCCAAGCCAGATGGAGGCAATA
TTCACGAGTGCAGCCCACACGAGCGACGTAGTCTCCGAGACTATCGAGAAGCTTAGGAAGGCCTTTAAGAGGCTTAGATA
A

Upstream 100 bases:

>100_bases
GGGCCTCCAACAACCTATTAGCAGTGACTGTCCTCGTGGATATTCGGCGTTCACAGTATAAGGGACCTAAGCGGCTCCTG
AATGTTTGAGGGCTCCCGCT

Downstream 100 bases:

>100_bases
CCATGAAGCTCCGCGTAGCTACGCGGGGTAGTAAGCTCAGCATAGCACAGACAATGATAGCTCTGGAGGCAATAAAACGT
GTCGAGCCAAGCCTAGAGTA

Product: glutamate-1-semialdehyde aminotransferase

Products: NA

Alternate protein names: GSA; Glutamate-1-semialdehyde aminotransferase; GSA-AT

Number of amino acids: Translated: 426; Mature: 425

Protein sequence:

>426_residues
MPGEVSRSLYEKALTLFPGGVNSPVRAAVKPYPFYVERAEGPYIYTVDGEKLIDYVLAYGPLILGHKHPRVEEAVRRQLE
KGWLYGAPYELEIRLAEKILKYYHPGGMVRFVNTGTEATMTAIRLARGVTGRKYIVKFNGCYHGAHDAVLVGAGSAAAEY
GVPTSKGIPEEVAKLTLVAKYNDIESVEKIMSKHGDEVAAIIVEPVAGNAGVIPPKKGFLQELRRIASEHGALLIMDEVI
TGFRLALGGAQEYYRVKADITTLGKIVGGGFPIGVVVADRKIMEHLTPSGKVFNAGTFNAHPVTMAAGLATIEVLEEGTP
YRVASEAGRALAEELESLVLSYGIDAAVNHVESMLQIFFVKGEVWSPEDAAKSDKKLYLKLHEELLKLGVFIAPSQMEAI
FTSAAHTSDVVSETIEKLRKAFKRLR

Sequences:

>Translated_426_residues
MPGEVSRSLYEKALTLFPGGVNSPVRAAVKPYPFYVERAEGPYIYTVDGEKLIDYVLAYGPLILGHKHPRVEEAVRRQLE
KGWLYGAPYELEIRLAEKILKYYHPGGMVRFVNTGTEATMTAIRLARGVTGRKYIVKFNGCYHGAHDAVLVGAGSAAAEY
GVPTSKGIPEEVAKLTLVAKYNDIESVEKIMSKHGDEVAAIIVEPVAGNAGVIPPKKGFLQELRRIASEHGALLIMDEVI
TGFRLALGGAQEYYRVKADITTLGKIVGGGFPIGVVVADRKIMEHLTPSGKVFNAGTFNAHPVTMAAGLATIEVLEEGTP
YRVASEAGRALAEELESLVLSYGIDAAVNHVESMLQIFFVKGEVWSPEDAAKSDKKLYLKLHEELLKLGVFIAPSQMEAI
FTSAAHTSDVVSETIEKLRKAFKRLR
>Mature_425_residues
PGEVSRSLYEKALTLFPGGVNSPVRAAVKPYPFYVERAEGPYIYTVDGEKLIDYVLAYGPLILGHKHPRVEEAVRRQLEK
GWLYGAPYELEIRLAEKILKYYHPGGMVRFVNTGTEATMTAIRLARGVTGRKYIVKFNGCYHGAHDAVLVGAGSAAAEYG
VPTSKGIPEEVAKLTLVAKYNDIESVEKIMSKHGDEVAAIIVEPVAGNAGVIPPKKGFLQELRRIASEHGALLIMDEVIT
GFRLALGGAQEYYRVKADITTLGKIVGGGFPIGVVVADRKIMEHLTPSGKVFNAGTFNAHPVTMAAGLATIEVLEEGTPY
RVASEAGRALAEELESLVLSYGIDAAVNHVESMLQIFFVKGEVWSPEDAAKSDKKLYLKLHEELLKLGVFIAPSQMEAIF
TSAAHTSDVVSETIEKLRKAFKRLR

Specific function: Porphyrin biosynthesis by the C5 pathway; second step. [C]

COG id: COG0001

COG function: function code H; Glutamate-1-semialdehyde aminotransferase

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-III pyridoxal-phosphate-dependent aminotransferase family. HemL subfamily

Homologues:

Organism=Homo sapiens, GI4557809, Length=323, Percent_Identity=30.9597523219814, Blast_Score=125, Evalue=1e-28,
Organism=Homo sapiens, GI13994255, Length=445, Percent_Identity=25.3932584269663, Blast_Score=108, Evalue=9e-24,
Organism=Homo sapiens, GI37574042, Length=367, Percent_Identity=26.7029972752044, Blast_Score=101, Evalue=1e-21,
Organism=Homo sapiens, GI24119277, Length=366, Percent_Identity=25.9562841530055, Blast_Score=97, Evalue=4e-20,
Organism=Homo sapiens, GI226442705, Length=360, Percent_Identity=26.1111111111111, Blast_Score=94, Evalue=3e-19,
Organism=Homo sapiens, GI226442709, Length=336, Percent_Identity=27.0833333333333, Blast_Score=93, Evalue=5e-19,
Organism=Homo sapiens, GI284507298, Length=232, Percent_Identity=33.6206896551724, Blast_Score=92, Evalue=7e-19,
Organism=Escherichia coli, GI1786349, Length=423, Percent_Identity=46.3356973995272, Blast_Score=383, Evalue=1e-107,
Organism=Escherichia coli, GI1789016, Length=364, Percent_Identity=29.6703296703297, Blast_Score=140, Evalue=2e-34,
Organism=Escherichia coli, GI1788044, Length=324, Percent_Identity=31.1728395061728, Blast_Score=136, Evalue=2e-33,
Organism=Escherichia coli, GI1787560, Length=403, Percent_Identity=28.5359801488834, Blast_Score=132, Evalue=6e-32,
Organism=Escherichia coli, GI145693181, Length=328, Percent_Identity=32.0121951219512, Blast_Score=129, Evalue=5e-31,
Organism=Escherichia coli, GI1789759, Length=296, Percent_Identity=29.7297297297297, Blast_Score=113, Evalue=2e-26,
Organism=Escherichia coli, GI1786991, Length=378, Percent_Identity=25.9259259259259, Blast_Score=96, Evalue=4e-21,
Organism=Caenorhabditis elegans, GI25144271, Length=328, Percent_Identity=27.7439024390244, Blast_Score=119, Evalue=2e-27,
Organism=Caenorhabditis elegans, GI32564660, Length=321, Percent_Identity=27.1028037383178, Blast_Score=107, Evalue=2e-23,
Organism=Caenorhabditis elegans, GI71992977, Length=334, Percent_Identity=27.5449101796407, Blast_Score=99, Evalue=6e-21,
Organism=Caenorhabditis elegans, GI25144274, Length=160, Percent_Identity=30.625, Blast_Score=81, Evalue=1e-15,
Organism=Saccharomyces cerevisiae, GI6323470, Length=329, Percent_Identity=27.6595744680851, Blast_Score=105, Evalue=1e-23,
Organism=Saccharomyces cerevisiae, GI6324432, Length=333, Percent_Identity=24.6246246246246, Blast_Score=92, Evalue=2e-19,
Organism=Drosophila melanogaster, GI21357415, Length=323, Percent_Identity=27.8637770897833, Blast_Score=110, Evalue=1e-24,
Organism=Drosophila melanogaster, GI161085790, Length=384, Percent_Identity=26.8229166666667, Blast_Score=107, Evalue=1e-23,
Organism=Drosophila melanogaster, GI28574759, Length=384, Percent_Identity=26.8229166666667, Blast_Score=107, Evalue=2e-23,
Organism=Drosophila melanogaster, GI21356575, Length=332, Percent_Identity=27.4096385542169, Blast_Score=92, Evalue=7e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): GSA_HYPBU (A2BL27)

Other databases:

- EMBL:   CP000493
- RefSeq:   YP_001013033.1
- ProteinModelPortal:   A2BL27
- SMR:   A2BL27
- STRING:   A2BL27
- GeneID:   4782262
- GenomeReviews:   CP000493_GR
- KEGG:   hbu:Hbut_0837
- eggNOG:   arNOG04972
- HOGENOM:   HBG725944
- OMA:   VDYAFSQ
- PhylomeDB:   A2BL27
- ProtClustDB:   PRK00062
- BioCyc:   HBUT415426:HBUT_0837-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00375
- InterPro:   IPR004639
- InterPro:   IPR005814
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422
- Gene3D:   G3DSA:3.40.640.10
- Gene3D:   G3DSA:3.90.1150.10
- PANTHER:   PTHR11986
- TIGRFAMs:   TIGR00713

Pfam domain/function: PF00202 Aminotran_3; SSF53383 PyrdxlP-dep_Trfase_major

EC number: =5.4.3.8

Molecular weight: Translated: 46427; Mature: 46296

Theoretical pI: Translated: 7.46; Mature: 7.46

Prosite motif: PS00600 AA_TRANSFER_CLASS_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPGEVSRSLYEKALTLFPGGVNSPVRAAVKPYPFYVERAEGPYIYTVDGEKLIDYVLAYG
CCCHHHHHHHHHHHHHCCCCCCCCHHHHCCCCCEEEEECCCCEEEEECHHHHHHHHHHHC
PLILGHKHPRVEEAVRRQLEKGWLYGAPYELEIRLAEKILKYYHPGGMVRFVNTGTEATM
CEEECCCCCCHHHHHHHHHHHCCCCCCCCEEEHHHHHHHHHHHCCCCEEEEEECCCHHHH
TAIRLARGVTGRKYIVKFNGCYHGAHDAVLVGAGSAAAEYGVPTSKGIPEEVAKLTLVAK
HHHHHHHCCCCCEEEEEEECEECCCCCEEEEECCCHHHHCCCCCCCCCHHHHHHEEEEEE
YNDIESVEKIMSKHGDEVAAIIVEPVAGNAGVIPPKKGFLQELRRIASEHGALLIMDEVI
CCCHHHHHHHHHHCCCCEEEEEEEECCCCCCCCCCCHHHHHHHHHHHHHCCCEEEEHHHH
TGFRLALGGAQEYYRVKADITTLGKIVGGGFPIGVVVADRKIMEHLTPSGKVFNAGTFNA
HHHHHHHCCCHHHEEEHHHHHHHHHHHCCCCCEEEEEECHHHHHHCCCCCCEEECCCCCC
HPVTMAAGLATIEVLEEGTPYRVASEAGRALAEELESLVLSYGIDAAVNHVESMLQIFFV
CCHHHHHHHHHHHHHHCCCCEEHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHEE
KGEVWSPEDAAKSDKKLYLKLHEELLKLGVFIAPSQMEAIFTSAAHTSDVVSETIEKLRK
CCCCCCCHHHCCCCHHHHHHHHHHHHHHCCEECCCHHHHHHHHHHHHHHHHHHHHHHHHH
AFKRLR
HHHHCC
>Mature Secondary Structure 
PGEVSRSLYEKALTLFPGGVNSPVRAAVKPYPFYVERAEGPYIYTVDGEKLIDYVLAYG
CCHHHHHHHHHHHHHCCCCCCCCHHHHCCCCCEEEEECCCCEEEEECHHHHHHHHHHHC
PLILGHKHPRVEEAVRRQLEKGWLYGAPYELEIRLAEKILKYYHPGGMVRFVNTGTEATM
CEEECCCCCCHHHHHHHHHHHCCCCCCCCEEEHHHHHHHHHHHCCCCEEEEEECCCHHHH
TAIRLARGVTGRKYIVKFNGCYHGAHDAVLVGAGSAAAEYGVPTSKGIPEEVAKLTLVAK
HHHHHHHCCCCCEEEEEEECEECCCCCEEEEECCCHHHHCCCCCCCCCHHHHHHEEEEEE
YNDIESVEKIMSKHGDEVAAIIVEPVAGNAGVIPPKKGFLQELRRIASEHGALLIMDEVI
CCCHHHHHHHHHHCCCCEEEEEEEECCCCCCCCCCCHHHHHHHHHHHHHCCCEEEEHHHH
TGFRLALGGAQEYYRVKADITTLGKIVGGGFPIGVVVADRKIMEHLTPSGKVFNAGTFNA
HHHHHHHCCCHHHEEEHHHHHHHHHHHCCCCCEEEEEECHHHHHHCCCCCCEEECCCCCC
HPVTMAAGLATIEVLEEGTPYRVASEAGRALAEELESLVLSYGIDAAVNHVESMLQIFFV
CCHHHHHHHHHHHHHHCCCCEEHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHEE
KGEVWSPEDAAKSDKKLYLKLHEELLKLGVFIAPSQMEAIFTSAAHTSDVVSETIEKLRK
CCCCCCCHHHCCCCHHHHHHHHHHHHHHCCEECCCHHHHHHHHHHHHHHHHHHHHHHHHH
AFKRLR
HHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA