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The map label for this gene is cobA [H]

Identifier: 124027711

GI number: 124027711

Start: 831701

End: 832447

Strand: Reverse

Name: cobA [H]

Synonym: Hbut_0835

Alternate gene names: 124027711

Gene position: 832447-831701 (Counterclockwise)

Preceding gene: 124027712

Following gene: 124027710

Centisome position: 49.93

GC content: 55.69

Gene sequence:

>747_bases
ATGCAATGCAGTGGTAGAGTCTACATCGTTGGTGCAGGTCCTGGAGACCCTGAGCTGATAACCGTCAAGGGGCTAAAGCT
GATCCGGGAAGCAGACGTCATAGTCTATGACAGGCTTATTCCGAGAGAGCTGCTAGAGGAAGCCAAGCCAGGTGCGAAGC
TGATATATGCTGGTAAGAGGCCAGGCATGCATGCTATGACGCAGGAGGAGATAAACAAGCTGCTCTACGAGCTGGCATGT
CAGGGTCTCACGGTCGTGAGGCTACACGGCGGGGACCCTTACGTCTTTGGTCGCGGCGAGGAGGAGTGCATGTACCTACG
GGAGCGTGGCGTAGACTGTGCGGTTGTGCCAGGGATTACCAGTGCCATAGCAGGCCCAGCCTATGCGGGTATACCCGTTA
CAAGCAGGTATGTAGCGTCAAGCTTTGCAGTTGTAACCGGGCGAGAAGCTGAGGGCAAGAAACAACGCATAGACTATGCA
GCCATAGCATCCGCCGTGGACACACTGGTCATACTCATGGGTGTAGGGAGACTTGAAGTGATAACCAGCCAGCTCCTAGA
GGCTGGCATAGATCCCGAGACTCCAGTCGCGATTGTTGAGAATGCTACAACTCCGGGCCAGCGCGTGGTAATCGGCACCC
TAAAGAACATAGCCGTGAAGGCACGCGAGGCGGGCATCGCGCCCCCGGCTGTCATTGTTATTGGTAGAGTGGTTGAGCTG
AGGGAGAAGCTTTGGCGGCTAAGTTAG

Upstream 100 bases:

>100_bases
AAGTAAGGGTCGAGGGTGATCCGGATAAGCCTACACAGGTAGGCCTAGAGGCAGCATTCGAGCTGAGGAGGCTAGCAGCA
AGGGAGGGGATAAGCCTGTA

Downstream 100 bases:

>100_bases
CGGGTAAGCCGACGATACTACTGCTTCGTCCTCCCGATTCCCAGCCGGGCCCACTAGCCGAGATAGGGTATGTGGCACAC
ATACCCATTGTAGAAGTCGT

Product: uroporphyrin-III C-methyltransferase

Products: NA

Alternate protein names: Urogen III methylase; SUMT; Uroporphyrinogen III methylase; UROM [H]

Number of amino acids: Translated: 248; Mature: 248

Protein sequence:

>248_residues
MQCSGRVYIVGAGPGDPELITVKGLKLIREADVIVYDRLIPRELLEEAKPGAKLIYAGKRPGMHAMTQEEINKLLYELAC
QGLTVVRLHGGDPYVFGRGEEECMYLRERGVDCAVVPGITSAIAGPAYAGIPVTSRYVASSFAVVTGREAEGKKQRIDYA
AIASAVDTLVILMGVGRLEVITSQLLEAGIDPETPVAIVENATTPGQRVVIGTLKNIAVKAREAGIAPPAVIVIGRVVEL
REKLWRLS

Sequences:

>Translated_248_residues
MQCSGRVYIVGAGPGDPELITVKGLKLIREADVIVYDRLIPRELLEEAKPGAKLIYAGKRPGMHAMTQEEINKLLYELAC
QGLTVVRLHGGDPYVFGRGEEECMYLRERGVDCAVVPGITSAIAGPAYAGIPVTSRYVASSFAVVTGREAEGKKQRIDYA
AIASAVDTLVILMGVGRLEVITSQLLEAGIDPETPVAIVENATTPGQRVVIGTLKNIAVKAREAGIAPPAVIVIGRVVEL
REKLWRLS
>Mature_248_residues
MQCSGRVYIVGAGPGDPELITVKGLKLIREADVIVYDRLIPRELLEEAKPGAKLIYAGKRPGMHAMTQEEINKLLYELAC
QGLTVVRLHGGDPYVFGRGEEECMYLRERGVDCAVVPGITSAIAGPAYAGIPVTSRYVASSFAVVTGREAEGKKQRIDYA
AIASAVDTLVILMGVGRLEVITSQLLEAGIDPETPVAIVENATTPGQRVVIGTLKNIAVKAREAGIAPPAVIVIGRVVEL
REKLWRLS

Specific function: Catalyzes both methylations at C-2 and C-7 of uroporphyrinogen III leading to precorrin-1 and precorrin-2; their oxidative esterification gives respectively factor I octamethyl ester and sirohydrochlorin [H]

COG id: COG0007

COG function: function code H; Uroporphyrinogen-III methylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the precorrin methyltransferase family [H]

Homologues:

Organism=Escherichia coli, GI1789768, Length=244, Percent_Identity=45.4918032786885, Blast_Score=202, Evalue=2e-53,
Organism=Saccharomyces cerevisiae, GI6322922, Length=235, Percent_Identity=36.5957446808511, Blast_Score=147, Evalue=2e-36,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000878
- InterPro:   IPR014777
- InterPro:   IPR014776
- InterPro:   IPR006366
- InterPro:   IPR003043 [H]

Pfam domain/function: PF00590 TP_methylase [H]

EC number: =2.1.1.107 [H]

Molecular weight: Translated: 26588; Mature: 26588

Theoretical pI: Translated: 7.29; Mature: 7.29

Prosite motif: PS00839 SUMT_1 ; PS00840 SUMT_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQCSGRVYIVGAGPGDPELITVKGLKLIREADVIVYDRLIPRELLEEAKPGAKLIYAGKR
CCCCCEEEEEECCCCCCCEEEECCHHHHHHCCCCEEHHHHHHHHHHHCCCCCEEEEECCC
PGMHAMTQEEINKLLYELACQGLTVVRLHGGDPYVFGRGEEECMYLRERGVDCAVVPGIT
CCCCHHHHHHHHHHHHHHHHCCEEEEEEECCCEEEECCCHHHHHHHHHCCCCEEECCCCH
SAIAGPAYAGIPVTSRYVASSFAVVTGREAEGKKQRIDYAAIASAVDTLVILMGVGRLEV
HHHCCCCCCCCCCHHHHHHHHEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHH
ITSQLLEAGIDPETPVAIVENATTPGQRVVIGTLKNIAVKAREAGIAPPAVIVIGRVVEL
HHHHHHHCCCCCCCCEEEEECCCCCCCEEEEEEHHHHHHHHHHCCCCCHHHHHHHHHHHH
REKLWRLS
HHHHHCCC
>Mature Secondary Structure
MQCSGRVYIVGAGPGDPELITVKGLKLIREADVIVYDRLIPRELLEEAKPGAKLIYAGKR
CCCCCEEEEEECCCCCCCEEEECCHHHHHHCCCCEEHHHHHHHHHHHCCCCCEEEEECCC
PGMHAMTQEEINKLLYELACQGLTVVRLHGGDPYVFGRGEEECMYLRERGVDCAVVPGIT
CCCCHHHHHHHHHHHHHHHHCCEEEEEEECCCEEEECCCHHHHHHHHHCCCCEEECCCCH
SAIAGPAYAGIPVTSRYVASSFAVVTGREAEGKKQRIDYAAIASAVDTLVILMGVGRLEV
HHHCCCCCCCCCCHHHHHHHHEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHH
ITSQLLEAGIDPETPVAIVENATTPGQRVVIGTLKNIAVKAREAGIAPPAVIVIGRVVEL
HHHHHHHCCCCCCCCEEEEECCCCCCCEEEEEEHHHHHHHHHHCCCCCHHHHHHHHHHHH
REKLWRLS
HHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8688087 [H]