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The map label for this gene is ynfE [H]

Identifier: 124027269

GI number: 124027269

Start: 363077

End: 365650

Strand: Reverse

Name: ynfE [H]

Synonym: Hbut_0373

Alternate gene names: 124027269

Gene position: 365650-363077 (Counterclockwise)

Preceding gene: 124027270

Following gene: 124027268

Centisome position: 21.93

GC content: 47.86

Gene sequence:

>2574_bases
GTGAAGATATCACGGCGTGATTTTATAAAGATCGCTGCTGTGGCAGGAGCATCAGTAGCACTACCCCTTTCGCTGCCAAG
GCCTCGGACGGTTATCGATAAAGGTGGTGTAAAGTTCGCAGCAGCTGCTAGCGAGCCTTCTAAATCTAAAGTAGTTGTTA
GCAGTTGTCTAATCTGTGGTCAACGCTGCCCGTTCAAGATTTACGTGCGTAAAGTTGGCGATGAGGAAGTAATAGAGAAG
ATAGTATATGCATCCGATCCTTCACATGATGAGTTCTTCGCTATCTGTGGCAGGCCACAAACCATATTCGAGCTGCGATA
TTTGCCGGAGCGAATACCTAGGCCCCTAAAGAGAGTCGGTCCTCGTGGTAGTGGCCAATTCGTGGAGATAACGTGGGAGG
AAGCACTTGACATCATAGCTAAGAAAATAAAAGAACTCATGGATAAGGGTGAGGTACACCGCATAGTCGCGGTATCCCAC
CAGGGTTACGAAGGTAGTCATGTAAGAGACTTCTTCAAGAACGTTATTGGCACTCCCAATGCTACTCAGCACTGTGATAC
TTGCCACACAGGCATGGATATCGGCCACAAGTTCATATTCGGCAGCCCAAAGGGGCCCTCCGCGTTTCAGCCAGACTATA
AGAATGCCAAGATGATCATCGTTATGGGCCGCAACCCAGTAGGAGGAATTGTTGCCTCAGCATGGACAAAGATGTTCACC
GAAGGTAGAGCCAATAAGGCAAAGCTTGTGGTATTCGATGTGAGGAAGAGCCGGCTCACAGCGATAGCCGACGAATACTA
CATAATACCTCCTGCAACCGATTTAGCTATATCACTCGCTATTCTCAACGTGATACTTACCGAAAAGCTCTACAATAAAG
ACTATCTAATCAAGTGGACTAACGCGCCAATGCTCTTCTACGCGGACACCCTAGAACCTGTCAAGCTCGCCGATAATCCG
TTGATGGAGGGTAAGAAGACGTATCTAGTATACGACGAGGCTACCGGCGAGTTCAAGCTGAAGACAGAAGCTAAACAGCC
AGCGCTAGAGTATGAAGGTACCTACAATGGCAGGCCTGTAAAGACAGCCCTTCTCCTACTGAAGGATCATGTGAAAGACT
ATACACCTGAATGGGCTGAAAAGATTACAGGTGTACCAGCCAACACTATAAGGAGAATTGCAAGAGAGCTAGCTGAAGCA
GCACCCAAGGCCTTCATAGACCATGGCTACAAAGGTGCAAGATATTATAACGAGGGTATGCTGAAGAGAGTGAACATGCT
CATAAACGTGCTTATAGGTAGTGTTGGAAGCGTCGGAGGCGTAGCATATCCGGCTGGCAAACCAAAGATTAAGCATCCCT
TCGACATCATAGGAATAGAGAAAACGAAGCCCACCGGGCTATCCATACCCGACTACTGGGCCCAGAACGGTATTGAAAAT
ATTATAGGTAAGTGCTGGAGTCAGCTCCTCGTCAAAACAATAATGACGGGTAAGCCATACCCAATAGGTCTACTATTCAT
ACATCTCGAGAACCTCGTAGCCCATACGGTAAGCGGCAGGAAGTTCCTGGAAGCATTAAAGGATGAGAAGCGTGTTGAAC
TCATAGTGGTAGCTGACACAACCTTCAATGAGACGGTGATGTATGCCGATATAGTACTCCCCATACCGCTCTTCTTCGAG
GCAACAACCTGGTCGCTGCAAACTGCTAAGAAGAGCTATGTTAGTCTTGTAACCACAGGCATAAAGGCTGTCGATCCACC
ATCCGGCATAGATGCTAGACCCACTTGGTGGATCCTTGTAGAACTCGCTAAGAGACTCGGCAAACTGCCACCAAACGTGG
AAGTTGACCCACTTGAGGTCCAGCGCAAGCAAGCTGAAGCACTAGGCATAAACTTCGACGAGCTGCTGGAGAAGGGTTAT
GTAATGCTATGGACGAAGCCAAAGTATCATCCATGGGGTGGTGAGCCTCTATCAACAGTTACCGGTGAACTCGAGATAGT
TAATGTGGCACTTCTAGAGAAGTATAGAGGGTTCGTCGGAAAAGAGTCGCCGCTGAACCCATTCCCTATATGGATACGGC
CACTCTGGATGAGGAAAGGCCAGCTGGCACCAGATGAGTTCATACCGGTAGAGTATCAGGACCCGCTAACATCGATAAAC
ATGCTCATAAGATTCGCGAAGCTCTCAATAGATGCTCTAGAATACAAGAACTTGTACGGTGTCCTAATACATCCAAGCAG
AGCCGAGAAGCTTGGGCTGAGGACAGGAGACCTGGTAAGGATTAAGGGTGCCGGAGGCGAAATAATTGCAAAGGTCATAG
TAAGTGAGGATGTACACCCATACATTATCGCAGCTCCACATGCAACAGCCATAGATGAGATGGTTATACCAAGGACAGCT
AGGATAAAGACACGGGAGGGCAGAGAGATCACAGTTAAACTCTTCAGCAAAGGAGGCGGTTATGGCATCAATAATAACCT
CCTTGCCGATCCTGATGAGGCTGTAGTTCCCGAAGAGGGTTATAGAGCTGCCCAGCATGATTTTGTCGTAAGAATAGAGA
AGATCACGGGATGA

Upstream 100 bases:

>100_bases
TACCTCAAAAAGCAGGGATACTACATAAATAACCCACATATCGATAAGTACTACTATAACGGCAGAGAATGGTGTAGCAT
CACGGGAGGGATGTATTGTC

Downstream 100 bases:

>100_bases
ATTAGCTGGCTAGGGGGTGGCTAGAATGGCTAGACTTGCAATGGTTATAGATCTCAATAGATGTACCGGCTGCATGGCGT
GTAGTATCGCATGTATAAGA

Product: anaerobic dehydrogenase, BisC

Products: NA

Alternate protein names: DMSO reductase [H]

Number of amino acids: Translated: 857; Mature: 857

Protein sequence:

>857_residues
MKISRRDFIKIAAVAGASVALPLSLPRPRTVIDKGGVKFAAAASEPSKSKVVVSSCLICGQRCPFKIYVRKVGDEEVIEK
IVYASDPSHDEFFAICGRPQTIFELRYLPERIPRPLKRVGPRGSGQFVEITWEEALDIIAKKIKELMDKGEVHRIVAVSH
QGYEGSHVRDFFKNVIGTPNATQHCDTCHTGMDIGHKFIFGSPKGPSAFQPDYKNAKMIIVMGRNPVGGIVASAWTKMFT
EGRANKAKLVVFDVRKSRLTAIADEYYIIPPATDLAISLAILNVILTEKLYNKDYLIKWTNAPMLFYADTLEPVKLADNP
LMEGKKTYLVYDEATGEFKLKTEAKQPALEYEGTYNGRPVKTALLLLKDHVKDYTPEWAEKITGVPANTIRRIARELAEA
APKAFIDHGYKGARYYNEGMLKRVNMLINVLIGSVGSVGGVAYPAGKPKIKHPFDIIGIEKTKPTGLSIPDYWAQNGIEN
IIGKCWSQLLVKTIMTGKPYPIGLLFIHLENLVAHTVSGRKFLEALKDEKRVELIVVADTTFNETVMYADIVLPIPLFFE
ATTWSLQTAKKSYVSLVTTGIKAVDPPSGIDARPTWWILVELAKRLGKLPPNVEVDPLEVQRKQAEALGINFDELLEKGY
VMLWTKPKYHPWGGEPLSTVTGELEIVNVALLEKYRGFVGKESPLNPFPIWIRPLWMRKGQLAPDEFIPVEYQDPLTSIN
MLIRFAKLSIDALEYKNLYGVLIHPSRAEKLGLRTGDLVRIKGAGGEIIAKVIVSEDVHPYIIAAPHATAIDEMVIPRTA
RIKTREGREITVKLFSKGGGYGINNNLLADPDEAVVPEEGYRAAQHDFVVRIEKITG

Sequences:

>Translated_857_residues
MKISRRDFIKIAAVAGASVALPLSLPRPRTVIDKGGVKFAAAASEPSKSKVVVSSCLICGQRCPFKIYVRKVGDEEVIEK
IVYASDPSHDEFFAICGRPQTIFELRYLPERIPRPLKRVGPRGSGQFVEITWEEALDIIAKKIKELMDKGEVHRIVAVSH
QGYEGSHVRDFFKNVIGTPNATQHCDTCHTGMDIGHKFIFGSPKGPSAFQPDYKNAKMIIVMGRNPVGGIVASAWTKMFT
EGRANKAKLVVFDVRKSRLTAIADEYYIIPPATDLAISLAILNVILTEKLYNKDYLIKWTNAPMLFYADTLEPVKLADNP
LMEGKKTYLVYDEATGEFKLKTEAKQPALEYEGTYNGRPVKTALLLLKDHVKDYTPEWAEKITGVPANTIRRIARELAEA
APKAFIDHGYKGARYYNEGMLKRVNMLINVLIGSVGSVGGVAYPAGKPKIKHPFDIIGIEKTKPTGLSIPDYWAQNGIEN
IIGKCWSQLLVKTIMTGKPYPIGLLFIHLENLVAHTVSGRKFLEALKDEKRVELIVVADTTFNETVMYADIVLPIPLFFE
ATTWSLQTAKKSYVSLVTTGIKAVDPPSGIDARPTWWILVELAKRLGKLPPNVEVDPLEVQRKQAEALGINFDELLEKGY
VMLWTKPKYHPWGGEPLSTVTGELEIVNVALLEKYRGFVGKESPLNPFPIWIRPLWMRKGQLAPDEFIPVEYQDPLTSIN
MLIRFAKLSIDALEYKNLYGVLIHPSRAEKLGLRTGDLVRIKGAGGEIIAKVIVSEDVHPYIIAAPHATAIDEMVIPRTA
RIKTREGREITVKLFSKGGGYGINNNLLADPDEAVVPEEGYRAAQHDFVVRIEKITG
>Mature_857_residues
MKISRRDFIKIAAVAGASVALPLSLPRPRTVIDKGGVKFAAAASEPSKSKVVVSSCLICGQRCPFKIYVRKVGDEEVIEK
IVYASDPSHDEFFAICGRPQTIFELRYLPERIPRPLKRVGPRGSGQFVEITWEEALDIIAKKIKELMDKGEVHRIVAVSH
QGYEGSHVRDFFKNVIGTPNATQHCDTCHTGMDIGHKFIFGSPKGPSAFQPDYKNAKMIIVMGRNPVGGIVASAWTKMFT
EGRANKAKLVVFDVRKSRLTAIADEYYIIPPATDLAISLAILNVILTEKLYNKDYLIKWTNAPMLFYADTLEPVKLADNP
LMEGKKTYLVYDEATGEFKLKTEAKQPALEYEGTYNGRPVKTALLLLKDHVKDYTPEWAEKITGVPANTIRRIARELAEA
APKAFIDHGYKGARYYNEGMLKRVNMLINVLIGSVGSVGGVAYPAGKPKIKHPFDIIGIEKTKPTGLSIPDYWAQNGIEN
IIGKCWSQLLVKTIMTGKPYPIGLLFIHLENLVAHTVSGRKFLEALKDEKRVELIVVADTTFNETVMYADIVLPIPLFFE
ATTWSLQTAKKSYVSLVTTGIKAVDPPSGIDARPTWWILVELAKRLGKLPPNVEVDPLEVQRKQAEALGINFDELLEKGY
VMLWTKPKYHPWGGEPLSTVTGELEIVNVALLEKYRGFVGKESPLNPFPIWIRPLWMRKGQLAPDEFIPVEYQDPLTSIN
MLIRFAKLSIDALEYKNLYGVLIHPSRAEKLGLRTGDLVRIKGAGGEIIAKVIVSEDVHPYIIAAPHATAIDEMVIPRTA
RIKTREGREITVKLFSKGGGYGINNNLLADPDEAVVPEEGYRAAQHDFVVRIEKITG

Specific function: Terminal reductase during anaerobic growth on various sulfoxide and N-oxide compounds [H]

COG id: COG0243

COG function: function code C; Anaerobic dehydrogenases, typically selenocysteine-containing

Gene ontology:

Cell location: Cell membrane; Peripheral membrane protein; Cytoplasmic side [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the prokaryotic molybdopterin-containing oxidoreductase family [H]

Homologues:

Organism=Escherichia coli, GI1787870, Length=846, Percent_Identity=25.531914893617, Blast_Score=176, Evalue=8e-45,
Organism=Escherichia coli, GI87081797, Length=828, Percent_Identity=25.3623188405797, Blast_Score=171, Evalue=2e-43,
Organism=Escherichia coli, GI171474008, Length=831, Percent_Identity=24.4283995186522, Blast_Score=168, Evalue=1e-42,
Organism=Escherichia coli, GI3868720, Length=511, Percent_Identity=24.0704500978474, Blast_Score=106, Evalue=5e-24,
Organism=Escherichia coli, GI3868719, Length=497, Percent_Identity=25.5533199195171, Blast_Score=95, Evalue=2e-20,
Organism=Escherichia coli, GI1788534, Length=668, Percent_Identity=21.5568862275449, Blast_Score=64, Evalue=4e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011888
- InterPro:   IPR009010
- InterPro:   IPR006657
- InterPro:   IPR006656
- InterPro:   IPR006963
- InterPro:   IPR006655
- InterPro:   IPR006311 [H]

Pfam domain/function: PF04879 Molybdop_Fe4S4; PF00384 Molybdopterin; PF01568 Molydop_binding [H]

EC number: 1.8.99.- [C]

Molecular weight: Translated: 95419; Mature: 95419

Theoretical pI: Translated: 9.33; Mature: 9.33

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKISRRDFIKIAAVAGASVALPLSLPRPRTVIDKGGVKFAAAASEPSKSKVVVSSCLICG
CCCCHHHHEEEEEECCCCEEEEECCCCCCEEECCCCEEEEEECCCCCCCHHHHHHHHHHC
QRCPFKIYVRKVGDEEVIEKIVYASDPSHDEFFAICGRPQTIFELRYLPERIPRPLKRVG
CCCCEEEEEEECCCHHHHHHHHHCCCCCCCCEEEEECCCCHHHHHHHHHHHCCCHHHHCC
PRGSGQFVEITWEEALDIIAKKIKELMDKGEVHRIVAVSHQGYEGSHVRDFFKNVIGTPN
CCCCCCEEEEEHHHHHHHHHHHHHHHHCCCCEEEEEEEECCCCCCHHHHHHHHHHCCCCC
ATQHCDTCHTGMDIGHKFIFGSPKGPSAFQPDYKNAKMIIVMGRNPVGGIVASAWTKMFT
CCCCCHHHHCCCCCCCEEEECCCCCCCCCCCCCCCCEEEEEECCCCCCHHHHHHHHHHHH
EGRANKAKLVVFDVRKSRLTAIADEYYIIPPATDLAISLAILNVILTEKLYNKDYLIKWT
CCCCCCEEEEEEECCHHHHHEEECCEEEECCCHHHHHHHHHHHHHHHHHHCCCCEEEEEE
NAPMLFYADTLEPVKLADNPLMEGKKTYLVYDEATGEFKLKTEAKQPALEYEGTYNGRPV
CCCEEEEECCCCCEECCCCCCCCCCEEEEEEECCCCCEEEEECCCCCCEECCCCCCCCHH
KTALLLLKDHVKDYTPEWAEKITGVPANTIRRIARELAEAAPKAFIDHGYKGARYYNEGM
HHHHHHHHHHHHHCCHHHHHHHCCCCHHHHHHHHHHHHHHCHHHHHHCCCCCCHHHHHHH
LKRVNMLINVLIGSVGSVGGVAYPAGKPKIKHPFDIIGIEKTKPTGLSIPDYWAQNGIEN
HHHHHHHHHHHHHCCCCCCCEECCCCCCCCCCCCEEEEECCCCCCCCCCCHHHHHCCHHH
IIGKCWSQLLVKTIMTGKPYPIGLLFIHLENLVAHTVSGRKFLEALKDEKRVELIVVADT
HHHHHHHHHHHHHHHCCCCCCEEEEEEEHHHHHHHHCCHHHHHHHHCCCCCEEEEEEECC
TFNETVMYADIVLPIPLFFEATTWSLQTAKKSYVSLVTTGIKAVDPPSGIDARPTWWILV
CCCCEEEEEEEEECCCEEEECCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHH
ELAKRLGKLPPNVEVDPLEVQRKQAEALGINFDELLEKGYVMLWTKPKYHPWGGEPLSTV
HHHHHHCCCCCCCCCCHHHHHHHHHHHHCCCHHHHHHCCEEEEEECCCCCCCCCCCHHHC
TGELEIVNVALLEKYRGFVGKESPLNPFPIWIRPLWMRKGQLAPDEFIPVEYQDPLTSIN
CCCEEEEHHHHHHHHHHCCCCCCCCCCCCEEEEEEECCCCCCCCCCCCCCCCCCHHHHHH
MLIRFAKLSIDALEYKNLYGVLIHPSRAEKLGLRTGDLVRIKGAGGEIIAKVIVSEDVHP
HHHHHHHHHHHHEECCCEEEEEECCCCHHHCCCCCCCEEEEECCCHHEEHEEHHCCCCCC
YIIAAPHATAIDEMVIPRTARIKTREGREITVKLFSKGGGYGINNNLLADPDEAVVPEEG
EEEECCCCHHHHHHHCCCCCEEECCCCCEEEEEEEECCCCCCCCCCCCCCCCCCCCCCCC
YRAAQHDFVVRIEKITG
CCHHCCCEEEEEEEECC
>Mature Secondary Structure
MKISRRDFIKIAAVAGASVALPLSLPRPRTVIDKGGVKFAAAASEPSKSKVVVSSCLICG
CCCCHHHHEEEEEECCCCEEEEECCCCCCEEECCCCEEEEEECCCCCCCHHHHHHHHHHC
QRCPFKIYVRKVGDEEVIEKIVYASDPSHDEFFAICGRPQTIFELRYLPERIPRPLKRVG
CCCCEEEEEEECCCHHHHHHHHHCCCCCCCCEEEEECCCCHHHHHHHHHHHCCCHHHHCC
PRGSGQFVEITWEEALDIIAKKIKELMDKGEVHRIVAVSHQGYEGSHVRDFFKNVIGTPN
CCCCCCEEEEEHHHHHHHHHHHHHHHHCCCCEEEEEEEECCCCCCHHHHHHHHHHCCCCC
ATQHCDTCHTGMDIGHKFIFGSPKGPSAFQPDYKNAKMIIVMGRNPVGGIVASAWTKMFT
CCCCCHHHHCCCCCCCEEEECCCCCCCCCCCCCCCCEEEEEECCCCCCHHHHHHHHHHHH
EGRANKAKLVVFDVRKSRLTAIADEYYIIPPATDLAISLAILNVILTEKLYNKDYLIKWT
CCCCCCEEEEEEECCHHHHHEEECCEEEECCCHHHHHHHHHHHHHHHHHHCCCCEEEEEE
NAPMLFYADTLEPVKLADNPLMEGKKTYLVYDEATGEFKLKTEAKQPALEYEGTYNGRPV
CCCEEEEECCCCCEECCCCCCCCCCEEEEEEECCCCCEEEEECCCCCCEECCCCCCCCHH
KTALLLLKDHVKDYTPEWAEKITGVPANTIRRIARELAEAAPKAFIDHGYKGARYYNEGM
HHHHHHHHHHHHHCCHHHHHHHCCCCHHHHHHHHHHHHHHCHHHHHHCCCCCCHHHHHHH
LKRVNMLINVLIGSVGSVGGVAYPAGKPKIKHPFDIIGIEKTKPTGLSIPDYWAQNGIEN
HHHHHHHHHHHHHCCCCCCCEECCCCCCCCCCCCEEEEECCCCCCCCCCCHHHHHCCHHH
IIGKCWSQLLVKTIMTGKPYPIGLLFIHLENLVAHTVSGRKFLEALKDEKRVELIVVADT
HHHHHHHHHHHHHHHCCCCCCEEEEEEEHHHHHHHHCCHHHHHHHHCCCCCEEEEEEECC
TFNETVMYADIVLPIPLFFEATTWSLQTAKKSYVSLVTTGIKAVDPPSGIDARPTWWILV
CCCCEEEEEEEEECCCEEEECCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHH
ELAKRLGKLPPNVEVDPLEVQRKQAEALGINFDELLEKGYVMLWTKPKYHPWGGEPLSTV
HHHHHHCCCCCCCCCCHHHHHHHHHHHHCCCHHHHHHCCEEEEEECCCCCCCCCCCHHHC
TGELEIVNVALLEKYRGFVGKESPLNPFPIWIRPLWMRKGQLAPDEFIPVEYQDPLTSIN
CCCEEEEHHHHHHHHHHCCCCCCCCCCCCEEEEEEECCCCCCCCCCCCCCCCCCHHHHHH
MLIRFAKLSIDALEYKNLYGVLIHPSRAEKLGLRTGDLVRIKGAGGEIIAKVIVSEDVHP
HHHHHHHHHHHHEECCCEEEEEECCCCHHHCCCCCCCEEEEECCCHHEEHEEHHCCCCCC
YIIAAPHATAIDEMVIPRTARIKTREGREITVKLFSKGGGYGINNNLLADPDEAVVPEEG
EEEECCCCHHHHHHHCCCCCEEECCCCCEEEEEEEECCCCCCCCCCCCCCCCCCCCCCCC
YRAAQHDFVVRIEKITG
CCHHCCCEEEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: May Bind 4Fe-4S Cluster. [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9097039; 9278503 [H]