Click here to switch to the map view.

The map label for this gene is hmeA [H]

Identifier: 124027268

GI number: 124027268

Start: 362404

End: 363051

Strand: Reverse

Name: hmeA [H]

Synonym: Hbut_0372

Alternate gene names: 124027268

Gene position: 363051-362404 (Counterclockwise)

Preceding gene: 124027269

Following gene: 124027267

Centisome position: 21.78

GC content: 50.62

Gene sequence:

>648_bases
ATGGCTAGACTTGCAATGGTTATAGATCTCAATAGATGTACCGGCTGCATGGCGTGTAGTATCGCATGTATAAGAGAGAA
TATTGCTAGACAGTTTAGCGAAAATGTTGAACTTCCGGAGGGCAACGCTATATTCTACGCAAGGACGAAGCCTGCAATGA
TCCGTGGAGGGCCATTTGGCGATGTACCGTACTTCATACAGTGCATGCACTGTGAAAACCCGCCATGTGCCGCTGTCTGC
CCCACCGGGGCAACCTACAAGACTAAGGAAGGCGTCGTAATGCTCGACCATAGTAAGTGTATTGGCTGCCGTGCATGTGT
CATCGCGTGTCCCTACGCTGCGAGAACAGTGTACCGTGGCAAGATGAGAGGACCAGCACCGCATTCAGAAGCTCTAGCAC
CGGGATATCCCGACAAGTGCACGTTCTGCTATCATCGTGCTAAGGAAGGTGATGGTAAGTGGGTTCCAGCATGTGTCGAA
GCTTGTGCCTTCCAGGCAAGGTTCTTCGGTGACTTAGACGACCCGAACAGCATTGTACATAAGCTTGTAAGTGAAGGTTA
TGCTGTTGCACTAGCTCCAGAGCTTGGTACTAAACCCAAGCTCTTCTTCGTTCCTCCAGCCAAAACCCATGTGATGAGGA
GGAGGTGA

Upstream 100 bases:

>100_bases
GCTGTAGTTCCCGAAGAGGGTTATAGAGCTGCCCAGCATGATTTTGTCGTAAGAATAGAGAAGATCACGGGATGAATTAG
CTGGCTAGGGGGTGGCTAGA

Downstream 100 bases:

>100_bases
GCGACCTATGAGCCATGGTAGGGTTGCTAGTGGTGCTAGGCTAGGAGCGGCTGGAGCAGCCTTCATAATTGTCGGCGGCC
TGCTTACAGTACTATTCATC

Product: Fe-S cluster-containing hydrogenase component 1

Products: oxidized cytochrome C552; NH3 [C]

Alternate protein names: Hme subunit A [H]

Number of amino acids: Translated: 215; Mature: 214

Protein sequence:

>215_residues
MARLAMVIDLNRCTGCMACSIACIRENIARQFSENVELPEGNAIFYARTKPAMIRGGPFGDVPYFIQCMHCENPPCAAVC
PTGATYKTKEGVVMLDHSKCIGCRACVIACPYAARTVYRGKMRGPAPHSEALAPGYPDKCTFCYHRAKEGDGKWVPACVE
ACAFQARFFGDLDDPNSIVHKLVSEGYAVALAPELGTKPKLFFVPPAKTHVMRRR

Sequences:

>Translated_215_residues
MARLAMVIDLNRCTGCMACSIACIRENIARQFSENVELPEGNAIFYARTKPAMIRGGPFGDVPYFIQCMHCENPPCAAVC
PTGATYKTKEGVVMLDHSKCIGCRACVIACPYAARTVYRGKMRGPAPHSEALAPGYPDKCTFCYHRAKEGDGKWVPACVE
ACAFQARFFGDLDDPNSIVHKLVSEGYAVALAPELGTKPKLFFVPPAKTHVMRRR
>Mature_214_residues
ARLAMVIDLNRCTGCMACSIACIRENIARQFSENVELPEGNAIFYARTKPAMIRGGPFGDVPYFIQCMHCENPPCAAVCP
TGATYKTKEGVVMLDHSKCIGCRACVIACPYAARTVYRGKMRGPAPHSEALAPGYPDKCTFCYHRAKEGDGKWVPACVEA
CAFQARFFGDLDDPNSIVHKLVSEGYAVALAPELGTKPKLFFVPPAKTHVMRRR

Specific function: Has menaquinol-oxidizing activity. HmeA, hmeB and hmeE subunits may together catalyze electron transfer from menaquinol to cytochrome c [H]

COG id: COG0437

COG function: function code C; Fe-S-cluster-containing hydrogenase components 1

Gene ontology:

Cell location: Cell membrane; Peripheral membrane protein; Extracellular side [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 4Fe-4S ferredoxin-type domain [H]

Homologues:

Organism=Escherichia coli, GI2367345, Length=208, Percent_Identity=37.0192307692308, Blast_Score=115, Evalue=3e-27,
Organism=Escherichia coli, GI1787122, Length=217, Percent_Identity=32.258064516129, Blast_Score=95, Evalue=4e-21,
Organism=Escherichia coli, GI1787872, Length=217, Percent_Identity=31.7972350230415, Blast_Score=91, Evalue=6e-20,
Organism=Escherichia coli, GI226510944, Length=201, Percent_Identity=32.3383084577114, Blast_Score=87, Evalue=1e-18,
Organism=Escherichia coli, GI1789370, Length=182, Percent_Identity=32.4175824175824, Blast_Score=76, Evalue=2e-15,
Organism=Escherichia coli, GI1787749, Length=200, Percent_Identity=30.5, Blast_Score=74, Evalue=6e-15,
Organism=Escherichia coli, GI1789067, Length=168, Percent_Identity=33.9285714285714, Blast_Score=74, Evalue=9e-15,
Organism=Escherichia coli, GI1787478, Length=106, Percent_Identity=37.7358490566038, Blast_Score=72, Evalue=3e-14,
Organism=Escherichia coli, GI1787740, Length=106, Percent_Identity=37.7358490566038, Blast_Score=72, Evalue=3e-14,
Organism=Escherichia coli, GI2367245, Length=168, Percent_Identity=28.5714285714286, Blast_Score=70, Evalue=1e-13,
Organism=Escherichia coli, GI1788811, Length=164, Percent_Identity=29.2682926829268, Blast_Score=69, Evalue=2e-13,
Organism=Escherichia coli, GI87082179, Length=162, Percent_Identity=31.4814814814815, Blast_Score=67, Evalue=1e-12,
Organism=Escherichia coli, GI1790326, Length=194, Percent_Identity=27.8350515463918, Blast_Score=67, Evalue=1e-12,
Organism=Escherichia coli, GI1787963, Length=117, Percent_Identity=35.8974358974359, Blast_Score=65, Evalue=3e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017896
- InterPro:   IPR017900
- InterPro:   IPR006311 [H]

Pfam domain/function: NA

EC number: NA

Molecular weight: Translated: 23558; Mature: 23427

Theoretical pI: Translated: 8.44; Mature: 8.44

Prosite motif: PS00198 4FE4S_FERREDOXIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

7.4 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
11.2 %Cys+Met (Translated Protein)
7.5 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
10.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MARLAMVIDLNRCTGCMACSIACIRENIARQFSENVELPEGNAIFYARTKPAMIRGGPFG
CCEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEECCCCEEECCCCC
DVPYFIQCMHCENPPCAAVCPTGATYKTKEGVVMLDHSKCIGCRACVIACPYAARTVYRG
CCCCEEEEEECCCCCCEEECCCCCCEECCCCEEEEECCCCCCCEEHEEECCHHHHHHHHC
KMRGPAPHSEALAPGYPDKCTFCYHRAKEGDGKWVPACVEACAFQARFFGDLDDPNSIVH
CCCCCCCCCCCCCCCCCCCCEEEEECCCCCCCCCCHHHHHHHHHHHHHCCCCCCHHHHHH
KLVSEGYAVALAPELGTKPKLFFVPPAKTHVMRRR
HHHHCCCEEEEECCCCCCCEEEEECCCHHHHCCCC
>Mature Secondary Structure 
ARLAMVIDLNRCTGCMACSIACIRENIARQFSENVELPEGNAIFYARTKPAMIRGGPFG
CEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEECCCCEEECCCCC
DVPYFIQCMHCENPPCAAVCPTGATYKTKEGVVMLDHSKCIGCRACVIACPYAARTVYRG
CCCCEEEEEECCCCCCEEECCCCCCEECCCCEEEEECCCCCCCEEHEEECCHHHHHHHHC
KMRGPAPHSEALAPGYPDKCTFCYHRAKEGDGKWVPACVEACAFQARFFGDLDDPNSIVH
CCCCCCCCCCCCCCCCCCCCEEEEECCCCCCCCCCHHHHHHHHHHHHHCCCCCCHHHHHH
KLVSEGYAVALAPELGTKPKLFFVPPAKTHVMRRR
HHHHCCCEEEEECCCCCCCEEEEECCCHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: Fe [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: reduced cytochrome C552; nitrite [C]

Specific reaction: reduced cytochrome C552 + nitrite = oxidized cytochrome C552 + NH3 [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 9389475; 11952791 [H]