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The map label for this gene is xthA [H]

Identifier: 124025252

GI number: 124025252

Start: 489714

End: 490541

Strand: Reverse

Name: xthA [H]

Synonym: NATL1_05411

Alternate gene names: 124025252

Gene position: 490541-489714 (Counterclockwise)

Preceding gene: 124025257

Following gene: 124025251

Centisome position: 26.31

GC content: 34.18

Gene sequence:

>828_bases
TTGCTTGTTGCAACCTGGAATGTGAATTCTATTCGTTCAAGAATAGATCATGTCAAAGAATGGCTCATCACTAACAAGAT
TGACATTCTTTGCTTACAAGAAACAAAAACAGAGGATAAGTTTTTTCCAATTGAAAATTTTTCAGACCTTGGGTATGAGG
TATCAATCTCTGGCCAAAAGTCTTACAATGGAGTGGCAATAATAAGTCGATTCCCAATTAATAATATAAAGATTGGGTTT
AATGAAGTTATCAACGACTATCAAGATTTATCTATATTAAGTGAGCAAAAAAGAATTATAAGCGCTGATATCAATGATAT
TAGAATCATAAATGTTTATGTTCCAAATGGATCATCTATAAATTCAGATAAGTTTATTTATAAAAAAAAATGGCTGGAAT
GCCTACAGGTTTACCTAAGAGAAATCAATAAAAATAATACACCAATATGTTTACTAGGAGACTTTAATATTGCCCCTGAA
GATAGAGATATCCATACACCAAGTAGATATGAAGAGTCAATTATGGCCTCTTCCAAAGAGAGGGAATTACTCAAAGATGC
TTTAGGAGGGGAATTAGAAGATGTTTTTAGGATTTTTGAGCCGGGAGAGAAAAATTGGTCTTGGTGGGATTATCGCCATT
CAGCCTGGGAAAGAGACAAGGGTTGGAGGATCGATCATATTTACCTAACAGAAGATATTCTTAGTTGTGCCAATAGTTGT
TGGATCGACAAAGAACAAAGATCTAGAGAGAAACCTAGTGATCACGCACCTGTCGTTGTGGATATAAACTGGCCTCCATC
AGATATTGAAAATGAATTTTTCTTCTAA

Upstream 100 bases:

>100_bases
AAACCATAATTGGCCATTAATAAAACGTGCAGTTTGCTTCAAAACTAAACTTTCGTCTAAATTTCCATAATAATAACTTA
ATAAATAAAATTTTTAAGTC

Downstream 100 bases:

>100_bases
AAATTCATTTGTTTTCTATTTTCAAATCATCACAGAATTAACAAATCAAATATTTACAATTAGCAAAAAACAATTGAAAT
CAAAGGGCATAATATCCAAG

Product: exodeoxyribonuclease III

Products: NA

Alternate protein names: EXO III; Exonuclease III; AP endonuclease VI [H]

Number of amino acids: Translated: 275; Mature: 275

Protein sequence:

>275_residues
MLVATWNVNSIRSRIDHVKEWLITNKIDILCLQETKTEDKFFPIENFSDLGYEVSISGQKSYNGVAIISRFPINNIKIGF
NEVINDYQDLSILSEQKRIISADINDIRIINVYVPNGSSINSDKFIYKKKWLECLQVYLREINKNNTPICLLGDFNIAPE
DRDIHTPSRYEESIMASSKERELLKDALGGELEDVFRIFEPGEKNWSWWDYRHSAWERDKGWRIDHIYLTEDILSCANSC
WIDKEQRSREKPSDHAPVVVDINWPPSDIENEFFF

Sequences:

>Translated_275_residues
MLVATWNVNSIRSRIDHVKEWLITNKIDILCLQETKTEDKFFPIENFSDLGYEVSISGQKSYNGVAIISRFPINNIKIGF
NEVINDYQDLSILSEQKRIISADINDIRIINVYVPNGSSINSDKFIYKKKWLECLQVYLREINKNNTPICLLGDFNIAPE
DRDIHTPSRYEESIMASSKERELLKDALGGELEDVFRIFEPGEKNWSWWDYRHSAWERDKGWRIDHIYLTEDILSCANSC
WIDKEQRSREKPSDHAPVVVDINWPPSDIENEFFF
>Mature_275_residues
MLVATWNVNSIRSRIDHVKEWLITNKIDILCLQETKTEDKFFPIENFSDLGYEVSISGQKSYNGVAIISRFPINNIKIGF
NEVINDYQDLSILSEQKRIISADINDIRIINVYVPNGSSINSDKFIYKKKWLECLQVYLREINKNNTPICLLGDFNIAPE
DRDIHTPSRYEESIMASSKERELLKDALGGELEDVFRIFEPGEKNWSWWDYRHSAWERDKGWRIDHIYLTEDILSCANSC
WIDKEQRSREKPSDHAPVVVDINWPPSDIENEFFF

Specific function: Major apurinic-apyrimidinic endonuclease of E.coli. It removes the damaged DNA at cytosines and guanines by cleaving on the 3'-side of the AP site by a beta-elimination reaction. It exhibits 3'-5'-exonuclease, 3'-phosphomonoesterase, 3'-repair diesterase

COG id: COG0708

COG function: function code L; Exonuclease III

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the DNA repair enzymes AP/ExoA family [H]

Homologues:

Organism=Homo sapiens, GI18375505, Length=267, Percent_Identity=26.2172284644195, Blast_Score=104, Evalue=7e-23,
Organism=Homo sapiens, GI18375503, Length=267, Percent_Identity=26.2172284644195, Blast_Score=104, Evalue=7e-23,
Organism=Homo sapiens, GI18375501, Length=267, Percent_Identity=26.2172284644195, Blast_Score=104, Evalue=7e-23,
Organism=Escherichia coli, GI1788046, Length=266, Percent_Identity=32.7067669172932, Blast_Score=144, Evalue=7e-36,
Organism=Caenorhabditis elegans, GI71989536, Length=269, Percent_Identity=26.3940520446097, Blast_Score=84, Evalue=7e-17,
Organism=Drosophila melanogaster, GI221330655, Length=271, Percent_Identity=27.6752767527675, Blast_Score=89, Evalue=4e-18,
Organism=Drosophila melanogaster, GI17136678, Length=271, Percent_Identity=27.6752767527675, Blast_Score=87, Evalue=8e-18,

Paralogues:

None

Copy number: 900 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000097
- InterPro:   IPR020847
- InterPro:   IPR020848
- InterPro:   IPR005135
- InterPro:   IPR004808 [H]

Pfam domain/function: PF03372 Exo_endo_phos [H]

EC number: =3.1.11.2 [H]

Molecular weight: Translated: 32274; Mature: 32274

Theoretical pI: Translated: 4.69; Mature: 4.69

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
0.7 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
0.7 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLVATWNVNSIRSRIDHVKEWLITNKIDILCLQETKTEDKFFPIENFSDLGYEVSISGQK
CEEEEECHHHHHHHHHHHHHHHHCCCEEEEEEECCCCCCCEECCCCCCCCCEEEEECCCC
SYNGVAIISRFPINNIKIGFNEVINDYQDLSILSEQKRIISADINDIRIINVYVPNGSSI
CCCCEEEEEECCCCCEEECHHHHHCHHHHHHHHHHHHHHEECCCCCEEEEEEEECCCCCC
NSDKFIYKKKWLECLQVYLREINKNNTPICLLGDFNIAPEDRDIHTPSRYEESIMASSKE
CCCCHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCCCCCCCCCHHHHHHHHHCCHH
RELLKDALGGELEDVFRIFEPGEKNWSWWDYRHSAWERDKGWRIDHIYLTEDILSCANSC
HHHHHHHHCCCHHHHHHHHCCCCCCCCEECHHHHHCCCCCCCEEEEEEECHHHHHHHHHC
WIDKEQRSREKPSDHAPVVVDINWPPSDIENEFFF
CCCCHHHHCCCCCCCCCEEEECCCCCCCCCCCCCC
>Mature Secondary Structure
MLVATWNVNSIRSRIDHVKEWLITNKIDILCLQETKTEDKFFPIENFSDLGYEVSISGQK
CEEEEECHHHHHHHHHHHHHHHHCCCEEEEEEECCCCCCCEECCCCCCCCCEEEEECCCC
SYNGVAIISRFPINNIKIGFNEVINDYQDLSILSEQKRIISADINDIRIINVYVPNGSSI
CCCCEEEEEECCCCCEEECHHHHHCHHHHHHHHHHHHHHEECCCCCEEEEEEEECCCCCC
NSDKFIYKKKWLECLQVYLREINKNNTPICLLGDFNIAPEDRDIHTPSRYEESIMASSKE
CCCCHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCCCCCCCCCHHHHHHHHHCCHH
RELLKDALGGELEDVFRIFEPGEKNWSWWDYRHSAWERDKGWRIDHIYLTEDILSCANSC
HHHHHHHHCCCHHHHHHHHCCCCCCCCEECHHHHHCCCCCCCEEEEEEECHHHHHHHHHC
WIDKEQRSREKPSDHAPVVVDINWPPSDIENEFFF
CCCCHHHHCCCCCCCCCEEEECCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 3049539; 9097039; 9278503; 8948651; 7885481 [H]