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The map label for this gene is hemL [H]

Identifier: 124025251

GI number: 124025251

Start: 488146

End: 489432

Strand: Reverse

Name: hemL [H]

Synonym: NATL1_05401

Alternate gene names: 124025251

Gene position: 489432-488146 (Counterclockwise)

Preceding gene: 124025252

Following gene: 124025245

Centisome position: 26.25

GC content: 43.05

Gene sequence:

>1287_bases
TTGAACACAAAAAGATCTGAAGAGATTTTTGGTGCTGCAAAAAATTTGATGCCTGGTGGAGTCAGTTCACCTGTAAGGGC
TTTCAAATCTGTTGAAGGGGATCCAATCGTTTTTGATCGCGTTAAAGGTCCCTATGCTTGGGATGTTGATGGGAACAGAT
ACATCGACTATGTAGGCAGTTGGGGGCCTGCGATTTGTGGCCATGCTCACCCTGAGGTAATAGCAGCTCTTCAAGAAACA
CTTGAAAAAGGCACGAGCTTCGGAGCACCTTGTGTTCTGGAAAACCAGCTCGCTGAAATGGTTATAGATGCCGTACCGAG
CGTTGAAATGGTCCGCTTCGTCAATAGTGGTACTGAAGCATGTATGGCTGTTCTCCGTTTAATGAGAGCCTTCACTGGAA
GAGATAAAGTTATCAAGTTTGAGGGCTGCTATCACGGACATGCAGATATGTTTTTAGTGAAAGCTGGCTCAGGAGTAGCA
ACACTTGGTTTGCCAGACTCACCAGGTGTACCAAGAAGTACAACAGCCAATACACTTACTGCTCCTTATAACGATCTTGA
AGCAGTCAAAGAACTATTTGCTGAAAACCCTGATGCTATTTCAGGAGTCATACTGGAGCCTGTTGTGGGGAATGCAGGCT
TCATAACACCTGAGCCAGGATTCTTAGAAGGGTTAAGAGAGGTAACTCAAGAGAACGGTGCTCTTTTGGTTTTTGATGAG
GTAATGACTGGTTTTCGAATCAGCTATGGGGGAGCACAAGAGCGTTTTGGAGTTACTCCTGATTTGACGACTATGGGGAA
AGTAATTGGTGGTGGCCTTCCAGTAGGTGCTTATGGAGGCCGTAAAGAAATCATGTCAATGGTCTCACCATCCGGTCCAA
TGTATCAAGCGGGTACTTTGAGCGGAAATCCTCTTGCCATGACAGCAGGTATTAAAACTCTTGAACTGCTTAAACAAGAA
GGAACATATGAAAAATTAGAAGCTCTTACAAAAAAGCTTTTGGATGGGATCCTCACTGCTGCTAAAGAATCAAATATTCC
CATTTATGGTCAGAGCATTAGCGCTATGTTCGGTTTTTACTTATGTGAGGGCCCTGTTAGGAACTTTGAGGAAGCCAAAT
CTTCTGACACTGAACTATTCAGTAAAATTCATAGACTGATGCTTCAGAAGGGTGTTTATCTAGCTCCAAGTGCATTTGAA
GCTGGTTTTACATCACTTGCTCATTCTGAAGATGATATAAATGCGACAATAAAAGCTTTTCAAGAAATCTTCTCTGAAAT
CTCATAA

Upstream 100 bases:

>100_bases
TGCTGAACAGATTCAAAAATTTAGACGAATCAGTCACTTTTAGGCCATTATGACTGACTGTGTTTATTAGCTCATTCGCT
GAGGTGCTGTGACAGACGCG

Downstream 100 bases:

>100_bases
AAAAAGTCCAATTCAAGTTTTACTTGAATTGGACTTTTTTAGTAATTTTGAAAGGATAATTAGTTTTTTCCTCTAACAAT
TACAGGAACATTTCCTGAAC

Product: glutamate-1-semialdehyde aminotransferase

Products: NA

Alternate protein names: GSA; Glutamate-1-semialdehyde aminotransferase; GSA-AT [H]

Number of amino acids: Translated: 428; Mature: 428

Protein sequence:

>428_residues
MNTKRSEEIFGAAKNLMPGGVSSPVRAFKSVEGDPIVFDRVKGPYAWDVDGNRYIDYVGSWGPAICGHAHPEVIAALQET
LEKGTSFGAPCVLENQLAEMVIDAVPSVEMVRFVNSGTEACMAVLRLMRAFTGRDKVIKFEGCYHGHADMFLVKAGSGVA
TLGLPDSPGVPRSTTANTLTAPYNDLEAVKELFAENPDAISGVILEPVVGNAGFITPEPGFLEGLREVTQENGALLVFDE
VMTGFRISYGGAQERFGVTPDLTTMGKVIGGGLPVGAYGGRKEIMSMVSPSGPMYQAGTLSGNPLAMTAGIKTLELLKQE
GTYEKLEALTKKLLDGILTAAKESNIPIYGQSISAMFGFYLCEGPVRNFEEAKSSDTELFSKIHRLMLQKGVYLAPSAFE
AGFTSLAHSEDDINATIKAFQEIFSEIS

Sequences:

>Translated_428_residues
MNTKRSEEIFGAAKNLMPGGVSSPVRAFKSVEGDPIVFDRVKGPYAWDVDGNRYIDYVGSWGPAICGHAHPEVIAALQET
LEKGTSFGAPCVLENQLAEMVIDAVPSVEMVRFVNSGTEACMAVLRLMRAFTGRDKVIKFEGCYHGHADMFLVKAGSGVA
TLGLPDSPGVPRSTTANTLTAPYNDLEAVKELFAENPDAISGVILEPVVGNAGFITPEPGFLEGLREVTQENGALLVFDE
VMTGFRISYGGAQERFGVTPDLTTMGKVIGGGLPVGAYGGRKEIMSMVSPSGPMYQAGTLSGNPLAMTAGIKTLELLKQE
GTYEKLEALTKKLLDGILTAAKESNIPIYGQSISAMFGFYLCEGPVRNFEEAKSSDTELFSKIHRLMLQKGVYLAPSAFE
AGFTSLAHSEDDINATIKAFQEIFSEIS
>Mature_428_residues
MNTKRSEEIFGAAKNLMPGGVSSPVRAFKSVEGDPIVFDRVKGPYAWDVDGNRYIDYVGSWGPAICGHAHPEVIAALQET
LEKGTSFGAPCVLENQLAEMVIDAVPSVEMVRFVNSGTEACMAVLRLMRAFTGRDKVIKFEGCYHGHADMFLVKAGSGVA
TLGLPDSPGVPRSTTANTLTAPYNDLEAVKELFAENPDAISGVILEPVVGNAGFITPEPGFLEGLREVTQENGALLVFDE
VMTGFRISYGGAQERFGVTPDLTTMGKVIGGGLPVGAYGGRKEIMSMVSPSGPMYQAGTLSGNPLAMTAGIKTLELLKQE
GTYEKLEALTKKLLDGILTAAKESNIPIYGQSISAMFGFYLCEGPVRNFEEAKSSDTELFSKIHRLMLQKGVYLAPSAFE
AGFTSLAHSEDDINATIKAFQEIFSEIS

Specific function: Porphyrin biosynthesis by the C5 pathway; second step. [C]

COG id: COG0001

COG function: function code H; Glutamate-1-semialdehyde aminotransferase

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-III pyridoxal-phosphate-dependent aminotransferase family. HemL subfamily [H]

Homologues:

Organism=Homo sapiens, GI4557809, Length=305, Percent_Identity=30.4918032786885, Blast_Score=124, Evalue=1e-28,
Organism=Homo sapiens, GI37574042, Length=309, Percent_Identity=29.7734627831715, Blast_Score=109, Evalue=4e-24,
Organism=Homo sapiens, GI13994255, Length=335, Percent_Identity=25.9701492537313, Blast_Score=105, Evalue=7e-23,
Organism=Homo sapiens, GI24119277, Length=319, Percent_Identity=27.5862068965517, Blast_Score=103, Evalue=4e-22,
Organism=Homo sapiens, GI226442705, Length=308, Percent_Identity=28.8961038961039, Blast_Score=102, Evalue=6e-22,
Organism=Homo sapiens, GI226442709, Length=276, Percent_Identity=29.7101449275362, Blast_Score=94, Evalue=3e-19,
Organism=Homo sapiens, GI284507298, Length=150, Percent_Identity=34.6666666666667, Blast_Score=86, Evalue=5e-17,
Organism=Escherichia coli, GI1786349, Length=425, Percent_Identity=52.9411764705882, Blast_Score=480, Evalue=1e-137,
Organism=Escherichia coli, GI1789016, Length=354, Percent_Identity=33.6158192090396, Blast_Score=167, Evalue=2e-42,
Organism=Escherichia coli, GI1788044, Length=295, Percent_Identity=35.5932203389831, Blast_Score=142, Evalue=3e-35,
Organism=Escherichia coli, GI1789759, Length=316, Percent_Identity=31.0126582278481, Blast_Score=139, Evalue=5e-34,
Organism=Escherichia coli, GI145693181, Length=307, Percent_Identity=31.5960912052117, Blast_Score=130, Evalue=1e-31,
Organism=Escherichia coli, GI1787560, Length=361, Percent_Identity=29.6398891966759, Blast_Score=125, Evalue=6e-30,
Organism=Escherichia coli, GI1786991, Length=319, Percent_Identity=26.0188087774295, Blast_Score=86, Evalue=5e-18,
Organism=Caenorhabditis elegans, GI25144271, Length=319, Percent_Identity=27.2727272727273, Blast_Score=122, Evalue=3e-28,
Organism=Caenorhabditis elegans, GI71992977, Length=362, Percent_Identity=27.3480662983425, Blast_Score=117, Evalue=2e-26,
Organism=Caenorhabditis elegans, GI32564660, Length=327, Percent_Identity=28.4403669724771, Blast_Score=102, Evalue=5e-22,
Organism=Caenorhabditis elegans, GI25144274, Length=166, Percent_Identity=30.7228915662651, Blast_Score=86, Evalue=4e-17,
Organism=Saccharomyces cerevisiae, GI6323470, Length=330, Percent_Identity=26.6666666666667, Blast_Score=108, Evalue=2e-24,
Organism=Saccharomyces cerevisiae, GI6324432, Length=310, Percent_Identity=24.8387096774194, Blast_Score=80, Evalue=6e-16,
Organism=Drosophila melanogaster, GI21357415, Length=300, Percent_Identity=26, Blast_Score=99, Evalue=6e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004639
- InterPro:   IPR005814
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422 [H]

Pfam domain/function: PF00202 Aminotran_3 [H]

EC number: =5.4.3.8 [H]

Molecular weight: Translated: 45824; Mature: 45824

Theoretical pI: Translated: 4.66; Mature: 4.66

Prosite motif: PS00600 AA_TRANSFER_CLASS_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNTKRSEEIFGAAKNLMPGGVSSPVRAFKSVEGDPIVFDRVKGPYAWDVDGNRYIDYVGS
CCCCHHHHHHHHHHHCCCCCCCHHHHHHHCCCCCCEEEECCCCCEEEECCCCEEEEECCC
WGPAICGHAHPEVIAALQETLEKGTSFGAPCVLENQLAEMVIDAVPSVEMVRFVNSGTEA
CCCHHCCCCCHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHCCHHH
CMAVLRLMRAFTGRDKVIKFEGCYHGHADMFLVKAGSGVATLGLPDSPGVPRSTTANTLT
HHHHHHHHHHHCCCCCEEEEECCCCCCCCEEEEECCCCEEEECCCCCCCCCCCCCCCCCC
APYNDLEAVKELFAENPDAISGVILEPVVGNAGFITPEPGFLEGLREVTQENGALLVFDE
CCCHHHHHHHHHHCCCCCHHCCEEECCCCCCCCCCCCCCHHHHHHHHHHHCCCCEEEEHH
VMTGFRISYGGAQERFGVTPDLTTMGKVIGGGLPVGAYGGRKEIMSMVSPSGPMYQAGTL
HHCCEEEECCCCHHCCCCCCCHHHHHHHHCCCCCCCCCCCHHHHHHHHCCCCCCEECCCC
SGNPLAMTAGIKTLELLKQEGTYEKLEALTKKLLDGILTAAKESNIPIYGQSISAMFGFY
CCCCEEEECCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCCCEECCHHHHHHHHH
LCEGPVRNFEEAKSSDTELFSKIHRLMLQKGVYLAPSAFEAGFTSLAHSEDDINATIKAF
HHCCHHHHHHHHCCCHHHHHHHHHHHHHHCCCEECCCHHHHHHHHHHCCCHHHHHHHHHH
QEIFSEIS
HHHHHHCC
>Mature Secondary Structure
MNTKRSEEIFGAAKNLMPGGVSSPVRAFKSVEGDPIVFDRVKGPYAWDVDGNRYIDYVGS
CCCCHHHHHHHHHHHCCCCCCCHHHHHHHCCCCCCEEEECCCCCEEEECCCCEEEEECCC
WGPAICGHAHPEVIAALQETLEKGTSFGAPCVLENQLAEMVIDAVPSVEMVRFVNSGTEA
CCCHHCCCCCHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHCCHHH
CMAVLRLMRAFTGRDKVIKFEGCYHGHADMFLVKAGSGVATLGLPDSPGVPRSTTANTLT
HHHHHHHHHHHCCCCCEEEEECCCCCCCCEEEEECCCCEEEECCCCCCCCCCCCCCCCCC
APYNDLEAVKELFAENPDAISGVILEPVVGNAGFITPEPGFLEGLREVTQENGALLVFDE
CCCHHHHHHHHHHCCCCCHHCCEEECCCCCCCCCCCCCCHHHHHHHHHHHCCCCEEEEHH
VMTGFRISYGGAQERFGVTPDLTTMGKVIGGGLPVGAYGGRKEIMSMVSPSGPMYQAGTL
HHCCEEEECCCCHHCCCCCCCHHHHHHHHCCCCCCCCCCCHHHHHHHHCCCCCCEECCCC
SGNPLAMTAGIKTLELLKQEGTYEKLEALTKKLLDGILTAAKESNIPIYGQSISAMFGFY
CCCCEEEECCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCCCEECCHHHHHHHHH
LCEGPVRNFEEAKSSDTELFSKIHRLMLQKGVYLAPSAFEAGFTSLAHSEDDINATIKAF
HHCCHHHHHHHHCCCHHHHHHHHHHHHHHCCCEECCCHHHHHHHHHHCCCHHHHHHHHHH
QEIFSEIS
HHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA