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The map label for this gene is glcD [H]

Identifier: 124022406

GI number: 124022406

Start: 649997

End: 651472

Strand: Direct

Name: glcD [H]

Synonym: P9303_06971

Alternate gene names: 124022406

Gene position: 649997-651472 (Clockwise)

Preceding gene: 124022404

Following gene: 124022408

Centisome position: 24.23

GC content: 55.49

Gene sequence:

>1476_bases
ATGGTGTCCCACGACTGGACTGCTCTAGACAGGGACTTAAGGCGATTTTTGCCGAAGAAGTCGGTGGTCTCCAAGCGGCA
AGAGTTGCTCGTTTACGACTGTGACGGGTTAACCATCGATCGTCATTGTCCTCCTTTGGCAGTGTTGCCAGAGACCACTG
AGGAGGTGTCTCAGATCTTGGCTTGTTGTCATCGCCATGGCATCCCTTTTGTCGCTAGGGGTAGTGGTACGGGGCTCTCG
GGAGGTGCATTAGTGGAGCAAGAGGCGCTGTTGGTGGTGACCAGTCGCATGCGCAGGATCCTTTCGATGGACCTTGATAA
CCACACCATTACGGTGCAGCCAGGAGTGATCAATAGTTGGGTGACTCGGGCGGTCGCTGGCGAAGGGTTTTATTACGCCC
CTGATCCTTCCAGTCAGGTGGTGTGCAGCATCGGCGGCAATGTGGCCGAGAACTCAGGTGGTGTGCACTGTCTCAAATAT
GGCGTCACCAGTAATCATGTTCTGGGGCTTGAAGTAGTGCTTGCTGATGGCACGGTGACCCTCCTCGGTGGTGGATTGTC
GGAGCATGCGGAGCTCGACCTGCGTGGGGCATTTATTGGCAGCGAAGGCACCCTGGGGATCGCCACAGCGATCACTCTGC
GGCTCTTGCGGGCGCCGCAACATGTGACTGTGCTGCTCGCAGATTTCCCCACCATGGAGTCTGCTGGCGAGGCGGTCTGC
CAGGTCACAGCCGCCGGAGTTTTACCTGCGGGTATGGAGATCATGGACAACTTCATGATTAATGCAGTCAATGATCTGTT
TGGCTTCGACGAATACCCTCGTGATGCCGCGGCTGTGCTGCTGATTGAGCTCGATGGTCAGTCGGCGGAAGTCACTGTGG
CCGCAGATCAGGCCAGTGGACTTTGTAGCAAGGCAGGAGCGAGGACCATTCGTCGTGCAGAAGAGGCTGCTGACCGTGCC
TTGCTTTGGAAGGGACGCAAGTCAGCATTTGCTGCGGTTGGGCAGATTTCACCCACCTATTACGTGCAGGACGGTGTCGT
GCCACGCAGCACTTTGCCGAGGGTGCTGACGGCAATAGAGAGGCTTAGTCGAGAGTATGAGTTGCCGGTGGCCAATGTTT
TTCATGCTGGCGATGGCAATTTGCATCCTTTAATCCTCTACAACGCAGACACGCCTGATGTGGAGTCTCGTGTTCGGGAT
CTGGGGTCAGCCATTCTGCGTGAGTGTCTTGATGTTGGCGGCAGCATCACTGGTGAACATGGGGTTGGGGCTGATAAGCG
CTGTTATCTCGATTGGATGTTTGCAGCGGATGATCTGTCCACAATGAAACTGCTACGAAGAGCCTTCGATCCAGATGGAC
GGGCCAATCCTGACAAAATTTTCCCCACTCCAAAAAGCTGTGGTGAGTCGGCTCGGCGCCAGGTCATCTTGACTTCAGAA
GGTCTTGAGATGCCTTCAGAAGCCAAGGCATTCTGA

Upstream 100 bases:

>100_bases
TCGCCATCTTGACCCAAAACGGTCAGAAGCGGATGAACTGTGTCAGGCCATCCGTTTACTTCTGGTTTTGCTGCCTAGGT
TATTGGACAGATGCGCGGGC

Downstream 100 bases:

>100_bases
CTGCTCCGGAGGTGAATCCTTGACTGCGACTATTGCCTTAAATAGCCATGTCCTTCAATTGCCAAACAGTTCCGACATCT
CCTCATCGTCATCAATTGGA

Product: glycolate oxidase subunit glcD

Products: 2-oxo acid; H2O2

Alternate protein names: NA

Number of amino acids: Translated: 491; Mature: 491

Protein sequence:

>491_residues
MVSHDWTALDRDLRRFLPKKSVVSKRQELLVYDCDGLTIDRHCPPLAVLPETTEEVSQILACCHRHGIPFVARGSGTGLS
GGALVEQEALLVVTSRMRRILSMDLDNHTITVQPGVINSWVTRAVAGEGFYYAPDPSSQVVCSIGGNVAENSGGVHCLKY
GVTSNHVLGLEVVLADGTVTLLGGGLSEHAELDLRGAFIGSEGTLGIATAITLRLLRAPQHVTVLLADFPTMESAGEAVC
QVTAAGVLPAGMEIMDNFMINAVNDLFGFDEYPRDAAAVLLIELDGQSAEVTVAADQASGLCSKAGARTIRRAEEAADRA
LLWKGRKSAFAAVGQISPTYYVQDGVVPRSTLPRVLTAIERLSREYELPVANVFHAGDGNLHPLILYNADTPDVESRVRD
LGSAILRECLDVGGSITGEHGVGADKRCYLDWMFAADDLSTMKLLRRAFDPDGRANPDKIFPTPKSCGESARRQVILTSE
GLEMPSEAKAF

Sequences:

>Translated_491_residues
MVSHDWTALDRDLRRFLPKKSVVSKRQELLVYDCDGLTIDRHCPPLAVLPETTEEVSQILACCHRHGIPFVARGSGTGLS
GGALVEQEALLVVTSRMRRILSMDLDNHTITVQPGVINSWVTRAVAGEGFYYAPDPSSQVVCSIGGNVAENSGGVHCLKY
GVTSNHVLGLEVVLADGTVTLLGGGLSEHAELDLRGAFIGSEGTLGIATAITLRLLRAPQHVTVLLADFPTMESAGEAVC
QVTAAGVLPAGMEIMDNFMINAVNDLFGFDEYPRDAAAVLLIELDGQSAEVTVAADQASGLCSKAGARTIRRAEEAADRA
LLWKGRKSAFAAVGQISPTYYVQDGVVPRSTLPRVLTAIERLSREYELPVANVFHAGDGNLHPLILYNADTPDVESRVRD
LGSAILRECLDVGGSITGEHGVGADKRCYLDWMFAADDLSTMKLLRRAFDPDGRANPDKIFPTPKSCGESARRQVILTSE
GLEMPSEAKAF
>Mature_491_residues
MVSHDWTALDRDLRRFLPKKSVVSKRQELLVYDCDGLTIDRHCPPLAVLPETTEEVSQILACCHRHGIPFVARGSGTGLS
GGALVEQEALLVVTSRMRRILSMDLDNHTITVQPGVINSWVTRAVAGEGFYYAPDPSSQVVCSIGGNVAENSGGVHCLKY
GVTSNHVLGLEVVLADGTVTLLGGGLSEHAELDLRGAFIGSEGTLGIATAITLRLLRAPQHVTVLLADFPTMESAGEAVC
QVTAAGVLPAGMEIMDNFMINAVNDLFGFDEYPRDAAAVLLIELDGQSAEVTVAADQASGLCSKAGARTIRRAEEAADRA
LLWKGRKSAFAAVGQISPTYYVQDGVVPRSTLPRVLTAIERLSREYELPVANVFHAGDGNLHPLILYNADTPDVESRVRD
LGSAILRECLDVGGSITGEHGVGADKRCYLDWMFAADDLSTMKLLRRAFDPDGRANPDKIFPTPKSCGESARRQVILTSE
GLEMPSEAKAF

Specific function: Unknown

COG id: COG0277

COG function: function code C; FAD/FMN-containing dehydrogenases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 FAD-binding PCMH-type domain [H]

Homologues:

Organism=Homo sapiens, GI37595756, Length=432, Percent_Identity=32.4074074074074, Blast_Score=218, Evalue=7e-57,
Organism=Homo sapiens, GI37595754, Length=455, Percent_Identity=30.7692307692308, Blast_Score=209, Evalue=4e-54,
Organism=Homo sapiens, GI119964728, Length=457, Percent_Identity=30.1969365426696, Blast_Score=161, Evalue=1e-39,
Organism=Homo sapiens, GI4501993, Length=452, Percent_Identity=21.2389380530973, Blast_Score=85, Evalue=2e-16,
Organism=Escherichia coli, GI1789351, Length=471, Percent_Identity=47.5583864118896, Blast_Score=434, Evalue=1e-123,
Organism=Escherichia coli, GI48994907, Length=445, Percent_Identity=23.5955056179775, Blast_Score=99, Evalue=6e-22,
Organism=Escherichia coli, GI1787977, Length=504, Percent_Identity=23.8095238095238, Blast_Score=97, Evalue=3e-21,
Organism=Caenorhabditis elegans, GI71992373, Length=432, Percent_Identity=30.5555555555556, Blast_Score=187, Evalue=2e-47,
Organism=Caenorhabditis elegans, GI71992381, Length=432, Percent_Identity=29.6296296296296, Blast_Score=167, Evalue=1e-41,
Organism=Caenorhabditis elegans, GI71992392, Length=364, Percent_Identity=30.2197802197802, Blast_Score=154, Evalue=7e-38,
Organism=Caenorhabditis elegans, GI17534361, Length=426, Percent_Identity=25.8215962441315, Blast_Score=132, Evalue=3e-31,
Organism=Caenorhabditis elegans, GI17556096, Length=218, Percent_Identity=25.6880733944954, Blast_Score=79, Evalue=4e-15,
Organism=Saccharomyces cerevisiae, GI6320027, Length=431, Percent_Identity=29.4663573085847, Blast_Score=184, Evalue=2e-47,
Organism=Saccharomyces cerevisiae, GI6320023, Length=461, Percent_Identity=26.4642082429501, Blast_Score=132, Evalue=1e-31,
Organism=Saccharomyces cerevisiae, GI6320764, Length=465, Percent_Identity=23.6559139784946, Blast_Score=123, Evalue=8e-29,
Organism=Drosophila melanogaster, GI18921117, Length=474, Percent_Identity=25.7383966244726, Blast_Score=123, Evalue=3e-28,
Organism=Drosophila melanogaster, GI24639277, Length=474, Percent_Identity=25.7383966244726, Blast_Score=123, Evalue=3e-28,
Organism=Drosophila melanogaster, GI24639275, Length=474, Percent_Identity=25.7383966244726, Blast_Score=123, Evalue=3e-28,
Organism=Drosophila melanogaster, GI24653753, Length=455, Percent_Identity=22.6373626373626, Blast_Score=91, Evalue=3e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016166
- InterPro:   IPR016167
- InterPro:   IPR016164
- InterPro:   IPR016168
- InterPro:   IPR004113
- InterPro:   IPR004490
- InterPro:   IPR006094
- InterPro:   IPR016171 [H]

Pfam domain/function: PF02913 FAD-oxidase_C; PF01565 FAD_binding_4 [H]

EC number: 1.1.3.15

Molecular weight: Translated: 52667; Mature: 52667

Theoretical pI: Translated: 5.03; Mature: 5.03

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.2 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVSHDWTALDRDLRRFLPKKSVVSKRQELLVYDCDGLTIDRHCPPLAVLPETTEEVSQIL
CCCCCHHHHHHHHHHHCCHHHHHHHHHCEEEECCCCEEECCCCCCEEECCCCHHHHHHHH
ACCHRHGIPFVARGSGTGLSGGALVEQEALLVVTSRMRRILSMDLDNHTITVQPGVINSW
HHHHHCCCCEEECCCCCCCCCCCEEHHHHHHHHHHHHHHHHHCCCCCCEEEECCCHHHHH
VTRAVAGEGFYYAPDPSSQVVCSIGGNVAENSGGVHCLKYGVTSNHVLGLEVVLADGTVT
HHHHHCCCCCEECCCCCCCEEEEECCCCCCCCCCEEEEEECCCCCCEEEEEEEEECCEEE
LLGGGLSEHAELDLRGAFIGSEGTLGIATAITLRLLRAPQHVTVLLADFPTMESAGEAVC
EEECCCCCCCCEEEEEEEECCCCCCHHHHHHHHHHHCCCCEEEEEEECCCCHHHHHHHHH
QVTAAGVLPAGMEIMDNFMINAVNDLFGFDEYPRDAAAVLLIELDGQSAEVTVAADQASG
HHHHHCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEEEECCCCEEEEEEECCCCC
LCSKAGARTIRRAEEAADRALLWKGRKSAFAAVGQISPTYYVQDGVVPRSTLPRVLTAIE
HHHHHHHHHHHHHHHHHHHHEEECCCHHHHHHHCCCCCEEEEECCCCCHHHHHHHHHHHH
RLSREYELPVANVFHAGDGNLHPLILYNADTPDVESRVRDLGSAILRECLDVGGSITGEH
HHHHHCCCCHHHEEECCCCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCC
GVGADKRCYLDWMFAADDLSTMKLLRRAFDPDGRANPDKIFPTPKSCGESARRQVILTSE
CCCCCCEEEEEEEEECCCHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHCEEEEECC
GLEMPSEAKAF
CCCCCCCCCCC
>Mature Secondary Structure
MVSHDWTALDRDLRRFLPKKSVVSKRQELLVYDCDGLTIDRHCPPLAVLPETTEEVSQIL
CCCCCHHHHHHHHHHHCCHHHHHHHHHCEEEECCCCEEECCCCCCEEECCCCHHHHHHHH
ACCHRHGIPFVARGSGTGLSGGALVEQEALLVVTSRMRRILSMDLDNHTITVQPGVINSW
HHHHHCCCCEEECCCCCCCCCCCEEHHHHHHHHHHHHHHHHHCCCCCCEEEECCCHHHHH
VTRAVAGEGFYYAPDPSSQVVCSIGGNVAENSGGVHCLKYGVTSNHVLGLEVVLADGTVT
HHHHHCCCCCEECCCCCCCEEEEECCCCCCCCCCEEEEEECCCCCCEEEEEEEEECCEEE
LLGGGLSEHAELDLRGAFIGSEGTLGIATAITLRLLRAPQHVTVLLADFPTMESAGEAVC
EEECCCCCCCCEEEEEEEECCCCCCHHHHHHHHHHHCCCCEEEEEEECCCCHHHHHHHHH
QVTAAGVLPAGMEIMDNFMINAVNDLFGFDEYPRDAAAVLLIELDGQSAEVTVAADQASG
HHHHHCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEEEECCCCEEEEEEECCCCC
LCSKAGARTIRRAEEAADRALLWKGRKSAFAAVGQISPTYYVQDGVVPRSTLPRVLTAIE
HHHHHHHHHHHHHHHHHHHHEEECCCHHHHHHHCCCCCEEEEECCCCCHHHHHHHHHHHH
RLSREYELPVANVFHAGDGNLHPLILYNADTPDVESRVRDLGSAILRECLDVGGSITGEH
HHHHHCCCCHHHEEECCCCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCC
GVGADKRCYLDWMFAADDLSTMKLLRRAFDPDGRANPDKIFPTPKSCGESARRQVILTSE
CCCCCCEEEEEEEEECCCHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHCEEEEECC
GLEMPSEAKAF
CCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: (S)-2-hydroxy acid; O2

Specific reaction: (S)-2-hydroxy acid + O2 = 2-oxo acid + H2O2

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12471157 [H]