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The map label for this gene is gatA [H]

Identifier: 124022404

GI number: 124022404

Start: 646582

End: 648360

Strand: Direct

Name: gatA [H]

Synonym: P9303_06951

Alternate gene names: 124022404

Gene position: 646582-648360 (Clockwise)

Preceding gene: 124022403

Following gene: 124022406

Centisome position: 24.1

GC content: 45.36

Gene sequence:

>1779_bases
ATGAATCATTCGAAGGGGTCGAAGATATTTAACCAAGGCATAAGGCTTGCGCTTGCTCTGAGCATGACTTGTCTTATGGC
TGAGAAGTCTATGGCCAACCAGTTTGGTAGTCAGAAATTTGGGATTAAGGAGTCTACAATTGAATCCATCCATGATGCTT
TTAAAGAGAGAGAGATTGATTGTGAGCAATTAATTCAGAGGTATTTATATCGAATCAAGAAGTATAATTTTAGCCTCGAA
AGAGGAGCTCCGCTTAATGCATTTGTTGCACTTAATCCGAATGCAGTCAGGCAGGCAAAGGCTCTGGATAGGCATTTTAA
ACAAAGCAATATATTGATTGGCCCTTTGCATTGCATCCCGATTGCTGTTAAAGACAACATTGATACCGTTGACACTCCTT
CCACTTCTGGGTCGTTGGCTTTGCTTGGCTCTCAGCCAATCAGTAATGCTTTTTTGGTGAATCAATTAAGAGCCGCTGGC
GGCATCATTATTGGTAAAGCCGCTATGGATGAATTTGCTTCTGGTGGGGAAGGAATTAGTGGTCGTAGCGGAAGAATTGG
CAATGCTTATGACCCTAATCAAAATTCCGGTGGCTCAAGTGGAGGCTCTGCGGTTGCGGTGAGTGCGAACTTTGCTGTGT
TGGGGATCGGCACGGACAACAGTGGGTCGGTCAGAGTGCCTGCAGCCTTCAACGGGGTTTATGCCATTCGCCCAAGCACG
GGGCTGATTAGTCATTCAGGAATTCTCCCGAGAGGCAACCTGGATGGAGTAGCAGGAGTGATGGCAAGGTCGATTCCTGA
TCTTGCTATTGGCCTTGCTGCGATAGCAAGCAGATCTGATCCCGATGATCATTTTACGAAACAAGTACCGCGCACAGATT
CTTATGCAAATAATTTAAAAACTGCTTCCCTTAATGGAAGGCGAATTGGCGTGATTCGCAGTGTTGCAGGCAATGAGGTC
TTTGACGCCACCGACAAGACTTCAATGGCTCTATTCAATCAGGTCAAAGCCAGGATTGAGAGAAATGGTGCATCACTTGT
TGAAATCAATCTGCCGTTATTCGACACGAACAGAGACAATAATATGGCGGGCGAGGCGGAAGATATTGATCAATACCTAG
GTTCTTTTGCGTCGACCAGAAGGAGCCTTCAGGATATATGCTTGTCTGGTCGCACAAGGCTAGGCGAGAAAGCCTGCATA
GGGTATATGGAAAGTATTGCGCCAAAGTACAGCGATCGATATGATTCTGCCTTAAAGACTTTTGAGAAGAATAGAAACTA
TGTAGAGGGATTGATGCGAGAAGATGGCATTGATGCTTTGCTAATGCCACTTAGTTCTTGGCAACCCCCAAGCTATTACG
ATGATATGTATCGCACAGCAACAACGGAATCGCCGGTTGCATCAAACTCCGGATTGCCGGCAATTGCTTTGATTGCTGGT
TGGACATCGGCAAGTCCAGCGATGCCTATCGGCTTTGAGTTGATTGGTTTTCAATATGGAGAGGGGGACCTAATCGGCCT
TGCGCAGGCTTATTCTTCTGGCCTTCCAGGCAGGCCTTTGCCTGAATTAAACGCAGGTAATAATGACTCTCCTTTCGAGG
ATATTTGTGTTCAAGGAATCAATTACTTTATTACTCAGGCTGGTTGGCATAGTTATAAGCAGTTCCTAAAGGATGCTAAT
GGTCAGAATATTGAGCCAGCTGCTTATCAAAGGTTTTTTGAGCAGCAAACGCAAAAATTCGCTCAGGCAAATCAACAGTC
TGTCCAAGCTTGTGAATAA

Upstream 100 bases:

>100_bases
GGTGCCGCTTCAATTTGAAAATTATCGATAATGAAACACTCTTGTGAATGTTGCTGTGTGTATAGTAGTGGCAGTAAAAT
TCTTTGTTTAAGTATTAGAC

Downstream 100 bases:

>100_bases
CTGCTCTGCTTATCTAGCTTTTTCCTGTTCTTGAGAGGCTTCTGCTCCGCGAAAACAGGCAAAATTAGGGCTTGCTAAAA
AACGAGACTTGAGTTAACTC

Product: Asp-tRNAAsn/Glu-tRNAGln amidotransferase subunit alpha

Products: NA

Alternate protein names: Glu-ADT subunit A [H]

Number of amino acids: Translated: 592; Mature: 592

Protein sequence:

>592_residues
MNHSKGSKIFNQGIRLALALSMTCLMAEKSMANQFGSQKFGIKESTIESIHDAFKEREIDCEQLIQRYLYRIKKYNFSLE
RGAPLNAFVALNPNAVRQAKALDRHFKQSNILIGPLHCIPIAVKDNIDTVDTPSTSGSLALLGSQPISNAFLVNQLRAAG
GIIIGKAAMDEFASGGEGISGRSGRIGNAYDPNQNSGGSSGGSAVAVSANFAVLGIGTDNSGSVRVPAAFNGVYAIRPST
GLISHSGILPRGNLDGVAGVMARSIPDLAIGLAAIASRSDPDDHFTKQVPRTDSYANNLKTASLNGRRIGVIRSVAGNEV
FDATDKTSMALFNQVKARIERNGASLVEINLPLFDTNRDNNMAGEAEDIDQYLGSFASTRRSLQDICLSGRTRLGEKACI
GYMESIAPKYSDRYDSALKTFEKNRNYVEGLMREDGIDALLMPLSSWQPPSYYDDMYRTATTESPVASNSGLPAIALIAG
WTSASPAMPIGFELIGFQYGEGDLIGLAQAYSSGLPGRPLPELNAGNNDSPFEDICVQGINYFITQAGWHSYKQFLKDAN
GQNIEPAAYQRFFEQQTQKFAQANQQSVQACE

Sequences:

>Translated_592_residues
MNHSKGSKIFNQGIRLALALSMTCLMAEKSMANQFGSQKFGIKESTIESIHDAFKEREIDCEQLIQRYLYRIKKYNFSLE
RGAPLNAFVALNPNAVRQAKALDRHFKQSNILIGPLHCIPIAVKDNIDTVDTPSTSGSLALLGSQPISNAFLVNQLRAAG
GIIIGKAAMDEFASGGEGISGRSGRIGNAYDPNQNSGGSSGGSAVAVSANFAVLGIGTDNSGSVRVPAAFNGVYAIRPST
GLISHSGILPRGNLDGVAGVMARSIPDLAIGLAAIASRSDPDDHFTKQVPRTDSYANNLKTASLNGRRIGVIRSVAGNEV
FDATDKTSMALFNQVKARIERNGASLVEINLPLFDTNRDNNMAGEAEDIDQYLGSFASTRRSLQDICLSGRTRLGEKACI
GYMESIAPKYSDRYDSALKTFEKNRNYVEGLMREDGIDALLMPLSSWQPPSYYDDMYRTATTESPVASNSGLPAIALIAG
WTSASPAMPIGFELIGFQYGEGDLIGLAQAYSSGLPGRPLPELNAGNNDSPFEDICVQGINYFITQAGWHSYKQFLKDAN
GQNIEPAAYQRFFEQQTQKFAQANQQSVQACE
>Mature_592_residues
MNHSKGSKIFNQGIRLALALSMTCLMAEKSMANQFGSQKFGIKESTIESIHDAFKEREIDCEQLIQRYLYRIKKYNFSLE
RGAPLNAFVALNPNAVRQAKALDRHFKQSNILIGPLHCIPIAVKDNIDTVDTPSTSGSLALLGSQPISNAFLVNQLRAAG
GIIIGKAAMDEFASGGEGISGRSGRIGNAYDPNQNSGGSSGGSAVAVSANFAVLGIGTDNSGSVRVPAAFNGVYAIRPST
GLISHSGILPRGNLDGVAGVMARSIPDLAIGLAAIASRSDPDDHFTKQVPRTDSYANNLKTASLNGRRIGVIRSVAGNEV
FDATDKTSMALFNQVKARIERNGASLVEINLPLFDTNRDNNMAGEAEDIDQYLGSFASTRRSLQDICLSGRTRLGEKACI
GYMESIAPKYSDRYDSALKTFEKNRNYVEGLMREDGIDALLMPLSSWQPPSYYDDMYRTATTESPVASNSGLPAIALIAG
WTSASPAMPIGFELIGFQYGEGDLIGLAQAYSSGLPGRPLPELNAGNNDSPFEDICVQGINYFITQAGWHSYKQFLKDAN
GQNIEPAAYQRFFEQQTQKFAQANQQSVQACE

Specific function: Furnishes a means for formation of correctly charged Gln-tRNA(Gln) through the transamidation of misacylated Glu- tRNA(Gln) in organisms which lack glutaminyl-tRNA synthetase. The reaction takes place in the presence of glutamine and ATP through an activa

COG id: COG0154

COG function: function code J; Asp-tRNAAsn/Glu-tRNAGln amidotransferase A subunit and related amidases

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the amidase family [H]

Homologues:

Organism=Homo sapiens, GI222831590, Length=514, Percent_Identity=27.2373540856031, Blast_Score=124, Evalue=2e-28,
Organism=Homo sapiens, GI195972892, Length=233, Percent_Identity=30.0429184549356, Blast_Score=96, Evalue=1e-19,
Organism=Caenorhabditis elegans, GI17543272, Length=412, Percent_Identity=26.4563106796117, Blast_Score=105, Evalue=1e-22,
Organism=Caenorhabditis elegans, GI17537465, Length=502, Percent_Identity=23.9043824701195, Blast_Score=91, Evalue=2e-18,
Organism=Caenorhabditis elegans, GI17556264, Length=223, Percent_Identity=26.457399103139, Blast_Score=79, Evalue=6e-15,
Organism=Caenorhabditis elegans, GI17538252, Length=201, Percent_Identity=25.8706467661692, Blast_Score=76, Evalue=5e-14,
Organism=Saccharomyces cerevisiae, GI6319685, Length=449, Percent_Identity=27.6169265033408, Blast_Score=103, Evalue=9e-23,
Organism=Saccharomyces cerevisiae, GI6323950, Length=409, Percent_Identity=23.7163814180929, Blast_Score=80, Evalue=9e-16,
Organism=Saccharomyces cerevisiae, GI6320448, Length=357, Percent_Identity=24.3697478991597, Blast_Score=71, Evalue=4e-13,
Organism=Drosophila melanogaster, GI24648113, Length=461, Percent_Identity=27.3318872017354, Blast_Score=114, Evalue=2e-25,
Organism=Drosophila melanogaster, GI24652985, Length=507, Percent_Identity=24.4575936883629, Blast_Score=86, Evalue=7e-17,
Organism=Drosophila melanogaster, GI19922090, Length=507, Percent_Identity=24.4575936883629, Blast_Score=86, Evalue=7e-17,
Organism=Drosophila melanogaster, GI24652981, Length=507, Percent_Identity=24.4575936883629, Blast_Score=86, Evalue=7e-17,
Organism=Drosophila melanogaster, GI24652983, Length=507, Percent_Identity=24.4575936883629, Blast_Score=86, Evalue=7e-17,
Organism=Drosophila melanogaster, GI21356731, Length=241, Percent_Identity=28.6307053941909, Blast_Score=86, Evalue=8e-17,
Organism=Drosophila melanogaster, GI45550774, Length=245, Percent_Identity=24.8979591836735, Blast_Score=83, Evalue=4e-16,
Organism=Drosophila melanogaster, GI24648435, Length=245, Percent_Identity=24.8979591836735, Blast_Score=83, Evalue=4e-16,
Organism=Drosophila melanogaster, GI24648437, Length=245, Percent_Identity=24.8979591836735, Blast_Score=83, Evalue=4e-16,
Organism=Drosophila melanogaster, GI24644968, Length=218, Percent_Identity=31.1926605504587, Blast_Score=83, Evalue=6e-16,
Organism=Drosophila melanogaster, GI24648441, Length=174, Percent_Identity=28.1609195402299, Blast_Score=75, Evalue=2e-13,
Organism=Drosophila melanogaster, GI24648439, Length=174, Percent_Identity=28.1609195402299, Blast_Score=75, Evalue=2e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000120
- InterPro:   IPR020556
- InterPro:   IPR004412 [H]

Pfam domain/function: PF01425 Amidase [H]

EC number: NA

Molecular weight: Translated: 63811; Mature: 63811

Theoretical pI: Translated: 6.42; Mature: 6.42

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNHSKGSKIFNQGIRLALALSMTCLMAEKSMANQFGSQKFGIKESTIESIHDAFKEREID
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHCCCC
CEQLIQRYLYRIKKYNFSLERGAPLNAFVALNPNAVRQAKALDRHFKQSNILIGPLHCIP
HHHHHHHHHHHHHHCCCCCCCCCCCEEEEEECCHHHHHHHHHHHHHHHCCCEEECCEEEE
IAVKDNIDTVDTPSTSGSLALLGSQPISNAFLVNQLRAAGGIIIGKAAMDEFASGGEGIS
EEEECCCCCCCCCCCCCCEEEECCCCCCHHHHHHHHHHHCCEEEEHHHHHHHHCCCCCCC
GRSGRIGNAYDPNQNSGGSSGGSAVAVSANFAVLGIGTDNSGSVRVPAAFNGVYAIRPST
CCCCCCCCCCCCCCCCCCCCCCCEEEEEECEEEEEECCCCCCCEECCEECCCEEEECCCC
GLISHSGILPRGNLDGVAGVMARSIPDLAIGLAAIASRSDPDDHFTKQVPRTDSYANNLK
CCEECCCCCCCCCCCHHHHHHHHCCHHHHHHHHHHHCCCCCCHHHHHCCCCCCHHHHCCE
TASLNGRRIGVIRSVAGNEVFDATDKTSMALFNQVKARIERNGASLVEINLPLFDTNRDN
EECCCCCEEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCEEEEEECCEEECCCCC
NMAGEAEDIDQYLGSFASTRRSLQDICLSGRTRLGEKACIGYMESIAPKYSDRYDSALKT
CCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHCCHHHHHHHHHHHH
FEKNRNYVEGLMREDGIDALLMPLSSWQPPSYYDDMYRTATTESPVASNSGLPAIALIAG
HHHCCHHHHHHHHHCCCCEEECCCCCCCCCCHHHHHHHHCCCCCCCCCCCCCCEEEEEEC
WTSASPAMPIGFELIGFQYGEGDLIGLAQAYSSGLPGRPLPELNAGNNDSPFEDICVQGI
CCCCCCCCCCCEEEEEEEECCCCEEEHHHHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHH
NYFITQAGWHSYKQFLKDANGQNIEPAAYQRFFEQQTQKFAQANQQSVQACE
HHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MNHSKGSKIFNQGIRLALALSMTCLMAEKSMANQFGSQKFGIKESTIESIHDAFKEREID
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHCCCC
CEQLIQRYLYRIKKYNFSLERGAPLNAFVALNPNAVRQAKALDRHFKQSNILIGPLHCIP
HHHHHHHHHHHHHHCCCCCCCCCCCEEEEEECCHHHHHHHHHHHHHHHCCCEEECCEEEE
IAVKDNIDTVDTPSTSGSLALLGSQPISNAFLVNQLRAAGGIIIGKAAMDEFASGGEGIS
EEEECCCCCCCCCCCCCCEEEECCCCCCHHHHHHHHHHHCCEEEEHHHHHHHHCCCCCCC
GRSGRIGNAYDPNQNSGGSSGGSAVAVSANFAVLGIGTDNSGSVRVPAAFNGVYAIRPST
CCCCCCCCCCCCCCCCCCCCCCCEEEEEECEEEEEECCCCCCCEECCEECCCEEEECCCC
GLISHSGILPRGNLDGVAGVMARSIPDLAIGLAAIASRSDPDDHFTKQVPRTDSYANNLK
CCEECCCCCCCCCCCHHHHHHHHCCHHHHHHHHHHHCCCCCCHHHHHCCCCCCHHHHCCE
TASLNGRRIGVIRSVAGNEVFDATDKTSMALFNQVKARIERNGASLVEINLPLFDTNRDN
EECCCCCEEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCEEEEEECCEEECCCCC
NMAGEAEDIDQYLGSFASTRRSLQDICLSGRTRLGEKACIGYMESIAPKYSDRYDSALKT
CCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHCCHHHHHHHHHHHH
FEKNRNYVEGLMREDGIDALLMPLSSWQPPSYYDDMYRTATTESPVASNSGLPAIALIAG
HHHCCHHHHHHHHHCCCCEEECCCCCCCCCCHHHHHHHHCCCCCCCCCCCCCCEEEEEEC
WTSASPAMPIGFELIGFQYGEGDLIGLAQAYSSGLPGRPLPELNAGNNDSPFEDICVQGI
CCCCCCCCCCCEEEEEEEECCCCEEEHHHHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHH
NYFITQAGWHSYKQFLKDANGQNIEPAAYQRFFEQQTQKFAQANQQSVQACE
HHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA