Click here to switch to the map view.

The map label for this gene is hisA

Identifier: 123966020

GI number: 123966020

Start: 708523

End: 709290

Strand: Direct

Name: hisA

Synonym: P9515_07851

Alternate gene names: 123966020

Gene position: 708523-709290 (Clockwise)

Preceding gene: 123966018

Following gene: 123966021

Centisome position: 41.58

GC content: 31.51

Gene sequence:

>768_bases
ATGAAATTAATTCCAGCAATAGATTTGATGAATGGTAAATGTGTAAGACTTTTTAAAGGTGACTTTAACAAGAGAAAAGA
CTTCTCTAGAAAACCTTATGAGCAAGCTAAATATTGGGAAGAACAGGGTGCAAAATGTATTCATATCGTTGATTTGGATG
CCGCTAAAAGCGGATATCCATCAAATGATCAATCAATTAAAAAAATTGCCAAAGAAGTCAATATTCCTATCCAAATTGGA
GGGGGTATAAGATCTTTAGAAAGGATTGAACAATTATTTTCATACGGTGTAGATAAGGTAATAATGGGTACCTCAGCTAT
TGAAAATAAAGAACTAGTTAAAAATCTATCAACCAAATTTCCAAGAAGAATAATTATTGGAATTGATGCAAAAGATGGGA
AAGTAAGCACTAGAGGATGGATAGAACAATCAGATGTATTAGCCACAGATTTAGTAAAGGAATTTTCCAAGTTTGAAATT
GCCAGTTTTATTGTCACCGACATCAATACTGATGGAACCTTAGAAGGGACAAATGAAGTTTTTATAAAAAAGATTCTTGA
AATTACTGATATTCCTGTTATCGCTTCAGGAGGAGTAGGTGCAATTTCTGATTTATTGTCATTAACCAAATTTGAACATT
TAGGACTATGTGGAGTAATAGTAGGAAAAGCACTTTATGAAAATAAATTTAAGATCAGTGAAGCTAATAACATATTATCT
CCTGAAAGATTACAAGATATTCCAATTAATAAGGACTATTTCGCTTGA

Upstream 100 bases:

>100_bases
ATTTTCATATTTCAAAAGTATTAAACAAAAGTAGTAATCTTAATTAGAACTACATAATGAATAGTACTACTAAGAAGTAA
TTAATGAAAAGGATGAATCA

Downstream 100 bases:

>100_bases
AAATACAAGAATTATTTATGTGGCACTGGTTTTTACAAGATTTGTTAGTTAATTTTGAATGAGAGGTAAACTTTGAGCAT
TGGAATTAATTAAGAAGAAA

Product: 1-(5-phosphoribosyl)-5-[(5- phosphoribosylamino)methylideneamino] imidazole-4-carboxamide isomerase

Products: NA

Alternate protein names: Phosphoribosylformimino-5-aminoimidazole carboxamide ribotide isomerase

Number of amino acids: Translated: 255; Mature: 255

Protein sequence:

>255_residues
MKLIPAIDLMNGKCVRLFKGDFNKRKDFSRKPYEQAKYWEEQGAKCIHIVDLDAAKSGYPSNDQSIKKIAKEVNIPIQIG
GGIRSLERIEQLFSYGVDKVIMGTSAIENKELVKNLSTKFPRRIIIGIDAKDGKVSTRGWIEQSDVLATDLVKEFSKFEI
ASFIVTDINTDGTLEGTNEVFIKKILEITDIPVIASGGVGAISDLLSLTKFEHLGLCGVIVGKALYENKFKISEANNILS
PERLQDIPINKDYFA

Sequences:

>Translated_255_residues
MKLIPAIDLMNGKCVRLFKGDFNKRKDFSRKPYEQAKYWEEQGAKCIHIVDLDAAKSGYPSNDQSIKKIAKEVNIPIQIG
GGIRSLERIEQLFSYGVDKVIMGTSAIENKELVKNLSTKFPRRIIIGIDAKDGKVSTRGWIEQSDVLATDLVKEFSKFEI
ASFIVTDINTDGTLEGTNEVFIKKILEITDIPVIASGGVGAISDLLSLTKFEHLGLCGVIVGKALYENKFKISEANNILS
PERLQDIPINKDYFA
>Mature_255_residues
MKLIPAIDLMNGKCVRLFKGDFNKRKDFSRKPYEQAKYWEEQGAKCIHIVDLDAAKSGYPSNDQSIKKIAKEVNIPIQIG
GGIRSLERIEQLFSYGVDKVIMGTSAIENKELVKNLSTKFPRRIIIGIDAKDGKVSTRGWIEQSDVLATDLVKEFSKFEI
ASFIVTDINTDGTLEGTNEVFIKKILEITDIPVIASGGVGAISDLLSLTKFEHLGLCGVIVGKALYENKFKISEANNILS
PERLQDIPINKDYFA

Specific function: Histidine biosynthesis; fourth step. [C]

COG id: COG0106

COG function: function code E; Phosphoribosylformimino-5-aminoimidazole carboxamide ribonucleotide (ProFAR) isomerase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the hisA/hisF family

Homologues:

Organism=Escherichia coli, GI87082028, Length=240, Percent_Identity=34.1666666666667, Blast_Score=151, Evalue=5e-38,
Organism=Escherichia coli, GI1788336, Length=257, Percent_Identity=25.6809338521401, Blast_Score=91, Evalue=8e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): HIS4_PROM5 (A2BW33)

Other databases:

- EMBL:   CP000552
- RefSeq:   YP_001011101.1
- ProteinModelPortal:   A2BW33
- SMR:   A2BW33
- STRING:   A2BW33
- GeneID:   4719450
- GenomeReviews:   CP000552_GR
- KEGG:   pmc:P9515_07851
- eggNOG:   COG0106
- HOGENOM:   HBG541613
- OMA:   SIIYTDI
- ProtClustDB:   PRK00748
- BioCyc:   PMAR167542:P9515ORF_0826-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_01014
- InterPro:   IPR013785
- InterPro:   IPR006062
- InterPro:   IPR006063
- InterPro:   IPR023016
- InterPro:   IPR011060
- Gene3D:   G3DSA:3.20.20.70
- TIGRFAMs:   TIGR00007

Pfam domain/function: PF00977 His_biosynth; SSF51366 RibP_bind_barrel

EC number: =5.3.1.16

Molecular weight: Translated: 28373; Mature: 28373

Theoretical pI: Translated: 8.03; Mature: 8.03

Prosite motif: NA

Important sites: ACT_SITE 8-8 ACT_SITE 129-129

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLIPAIDLMNGKCVRLFKGDFNKRKDFSRKPYEQAKYWEEQGAKCIHIVDLDAAKSGYP
CCCCCCEECCCCCEEEEECCCCCCCCCCCCCCHHHHHHHHHCCCCEEEEEEECCCCCCCC
SNDQSIKKIAKEVNIPIQIGGGIRSLERIEQLFSYGVDKVIMGTSAIENKELVKNLSTKF
CCHHHHHHHHHHCCCCEEECCCHHHHHHHHHHHHHCCHHEECCCCCCCHHHHHHHHHHCC
PRRIIIGIDAKDGKVSTRGWIEQSDVLATDLVKEFSKFEIASFIVTDINTDGTLEGTNEV
CCEEEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHEEEECCCCCCCCCCHHH
FIKKILEITDIPVIASGGVGAISDLLSLTKFEHLGLCGVIVGKALYENKFKISEANNILS
HHHHHHHHHCCCEEECCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCEECCCCCCCC
PERLQDIPINKDYFA
HHHHHCCCCCCCCCC
>Mature Secondary Structure
MKLIPAIDLMNGKCVRLFKGDFNKRKDFSRKPYEQAKYWEEQGAKCIHIVDLDAAKSGYP
CCCCCCEECCCCCEEEEECCCCCCCCCCCCCCHHHHHHHHHCCCCEEEEEEECCCCCCCC
SNDQSIKKIAKEVNIPIQIGGGIRSLERIEQLFSYGVDKVIMGTSAIENKELVKNLSTKF
CCHHHHHHHHHHCCCCEEECCCHHHHHHHHHHHHHCCHHEECCCCCCCHHHHHHHHHHCC
PRRIIIGIDAKDGKVSTRGWIEQSDVLATDLVKEFSKFEIASFIVTDINTDGTLEGTNEV
CCEEEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHEEEECCCCCCCCCCHHH
FIKKILEITDIPVIASGGVGAISDLLSLTKFEHLGLCGVIVGKALYENKFKISEANNILS
HHHHHHHHHCCCEEECCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCEECCCCCCCC
PERLQDIPINKDYFA
HHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA