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The map label for this gene is purH

Identifier: 123965534

GI number: 123965534

Start: 275364

End: 276917

Strand: Reverse

Name: purH

Synonym: P9515_02991

Alternate gene names: 123965534

Gene position: 276917-275364 (Counterclockwise)

Preceding gene: 123965536

Following gene: 123965529

Centisome position: 16.25

GC content: 34.23

Gene sequence:

>1554_bases
ATGTCACCATTAGCCCTAGTAAGTGTCTCTGATAAAACAAATATCATTCCATTTTGTAAGGATTTAGTTGAAAAATTTGG
TTATAATATTTTATCCAGCGGAGGGACCGCTGAGTACTTGACAGAGGCAAAAGTTCCTGTTCTTAAAGTAGCAGATTTTA
CTGAATCTCCAGAGATTCTTGACGGGAGAGTTAAAACTTTACATCCAAAGATTCATGGAGGAATTCTTGCTAAAAGATCT
AATGAAGAGCATCAAAGGGAAATATTAGAAAACAAACTAGAATTGATTGATTTGGTAGTTGTTAATTTGTATCCCTTTAA
GAAAAAAGTGGAAGAGCAATGTCCTTGGGAAGAAGCAATTGAGAATATTGATATAGGAGGTCCATCTATGATACGCTCTG
CAGCTAAAAATCATGCTGATGTTGCAGTTTTAGTAGATCCTAATCAATATCAAAATTATATTGAAGAGATCAAAAAAGGA
CCACTTAGCAAAGACTTTAAAACGAAATTAGCATTTGAGGCGTTTCAACATACCGCAAGTTATGACTCTGCAATATCAAA
TTGGATTAGTAAAGAAAAGGATTTAAGACCTTCGAATTTTATAGAATCATACCCGCTTATAAAGCAATTAAGGTATGGAG
AAAATCCCCATCAAAAAGCATTATGGTATGGATTAAATAATATTGGATGGAATTCAGCAGAACAATTACAAGGCAAAGAG
TTAAGCTATAACAACATACTCGATCTTGAATCAGCTCTATTAACTGTATTAGAATTTGGATATGAAACAAAGCCTAACAT
TAAGACCGAATCAATAGCTGCAGTTATTCTCAAACATAATAATCCTTGTGGGGCTTCGATTAGCAACTCAGCATCTAGTT
CTTTTAAGAATGCGTTAAAGTGCGATTCAGTTAGTGCCTTTGGGGGCATAGTAGCATTTAATGCCAATGTTGATAAAGAA
ACTGCCCTTATTCTGAAAGACATTTTTTTAGAGTGCGTAGTAGCACCATCCTTTGATAAAGAAGCTTTAGAAATATTTAA
AACCAAAAAGAATTTGAGAGTTTTAAAGTTAACAAAAGAAATGCTGCCTAAAGAAAACCAAACTTGTTCCAAATCAATTA
TGGGAGGAATACTCATACAAGATTCTGATAATCAGGAAAATTCAGAAGATTCTTGGATTTCAGTAACCAAAAAGAATCCA
ACTGAACAGGAATATTTAGATTTGAAATTTGCTTGGAAAATTTGTAAACATGTTAAGTCGAACGCTATTGTAGTTGCAAA
AGATCAACAAACTCTTGGCATAGGAGCTGGGCAAATGAATAGAGTTGGGGCTTCAAAAATAGCTTTAGAAGCAGCTAAAG
AAATTGATTCTGGAGGGGTTTTAGCAAGCGATGGTTTTTTCCCGTTCGCAGATACAGTGCGACTTGCAGATAAGTATGGA
ATAAGTTCTATTATTCAGCCGGGAGGTAGTATAAGAGATGAAGAAAGCATAAAAATGTGTGATTCAAGGGGTATTTCCAT
GATATTTACCCACAAAAGACACTTTTTACATTAA

Upstream 100 bases:

>100_bases
AAATGATTCTGTGCTTCGCTGTTTGAGAGCTAATGGAAACATATTCATGATTGATATCGTATTTCATGTTTATATTCTTA
AGTAATCAAGTTTTTTTGTA

Downstream 100 bases:

>100_bases
GTTAATTTGTTTTTGGATAATAAGTTATTTTATTAGTCTTACGAAGTAAAGCCAACCTTCCAGGCCCGGCACATAAAAAA
TAAAGAGAAATAATTCCATA

Product: bifunctional phosphoribosylaminoimidazolecarboxamide formyltransferase/IMP cyclohydrolase

Products: NA

Alternate protein names: Phosphoribosylaminoimidazolecarboxamide formyltransferase; AICAR transformylase; IMP cyclohydrolase; ATIC; IMP synthase; Inosinicase

Number of amino acids: Translated: 517; Mature: 516

Protein sequence:

>517_residues
MSPLALVSVSDKTNIIPFCKDLVEKFGYNILSSGGTAEYLTEAKVPVLKVADFTESPEILDGRVKTLHPKIHGGILAKRS
NEEHQREILENKLELIDLVVVNLYPFKKKVEEQCPWEEAIENIDIGGPSMIRSAAKNHADVAVLVDPNQYQNYIEEIKKG
PLSKDFKTKLAFEAFQHTASYDSAISNWISKEKDLRPSNFIESYPLIKQLRYGENPHQKALWYGLNNIGWNSAEQLQGKE
LSYNNILDLESALLTVLEFGYETKPNIKTESIAAVILKHNNPCGASISNSASSSFKNALKCDSVSAFGGIVAFNANVDKE
TALILKDIFLECVVAPSFDKEALEIFKTKKNLRVLKLTKEMLPKENQTCSKSIMGGILIQDSDNQENSEDSWISVTKKNP
TEQEYLDLKFAWKICKHVKSNAIVVAKDQQTLGIGAGQMNRVGASKIALEAAKEIDSGGVLASDGFFPFADTVRLADKYG
ISSIIQPGGSIRDEESIKMCDSRGISMIFTHKRHFLH

Sequences:

>Translated_517_residues
MSPLALVSVSDKTNIIPFCKDLVEKFGYNILSSGGTAEYLTEAKVPVLKVADFTESPEILDGRVKTLHPKIHGGILAKRS
NEEHQREILENKLELIDLVVVNLYPFKKKVEEQCPWEEAIENIDIGGPSMIRSAAKNHADVAVLVDPNQYQNYIEEIKKG
PLSKDFKTKLAFEAFQHTASYDSAISNWISKEKDLRPSNFIESYPLIKQLRYGENPHQKALWYGLNNIGWNSAEQLQGKE
LSYNNILDLESALLTVLEFGYETKPNIKTESIAAVILKHNNPCGASISNSASSSFKNALKCDSVSAFGGIVAFNANVDKE
TALILKDIFLECVVAPSFDKEALEIFKTKKNLRVLKLTKEMLPKENQTCSKSIMGGILIQDSDNQENSEDSWISVTKKNP
TEQEYLDLKFAWKICKHVKSNAIVVAKDQQTLGIGAGQMNRVGASKIALEAAKEIDSGGVLASDGFFPFADTVRLADKYG
ISSIIQPGGSIRDEESIKMCDSRGISMIFTHKRHFLH
>Mature_516_residues
SPLALVSVSDKTNIIPFCKDLVEKFGYNILSSGGTAEYLTEAKVPVLKVADFTESPEILDGRVKTLHPKIHGGILAKRSN
EEHQREILENKLELIDLVVVNLYPFKKKVEEQCPWEEAIENIDIGGPSMIRSAAKNHADVAVLVDPNQYQNYIEEIKKGP
LSKDFKTKLAFEAFQHTASYDSAISNWISKEKDLRPSNFIESYPLIKQLRYGENPHQKALWYGLNNIGWNSAEQLQGKEL
SYNNILDLESALLTVLEFGYETKPNIKTESIAAVILKHNNPCGASISNSASSSFKNALKCDSVSAFGGIVAFNANVDKET
ALILKDIFLECVVAPSFDKEALEIFKTKKNLRVLKLTKEMLPKENQTCSKSIMGGILIQDSDNQENSEDSWISVTKKNPT
EQEYLDLKFAWKICKHVKSNAIVVAKDQQTLGIGAGQMNRVGASKIALEAAKEIDSGGVLASDGFFPFADTVRLADKYGI
SSIIQPGGSIRDEESIKMCDSRGISMIFTHKRHFLH

Specific function: De novo purine biosynthesis; ninth step. De novo purine biosynthesis; tenth step. [C]

COG id: COG0138

COG function: function code F; AICAR transformylase/IMP cyclohydrolase PurH (only IMP cyclohydrolase domain in Aful)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the purH family

Homologues:

Organism=Homo sapiens, GI20127454, Length=487, Percent_Identity=34.9075975359343, Blast_Score=220, Evalue=3e-57,
Organism=Escherichia coli, GI1790439, Length=539, Percent_Identity=46.9387755102041, Blast_Score=437, Evalue=1e-124,
Organism=Caenorhabditis elegans, GI71985564, Length=608, Percent_Identity=32.2368421052632, Blast_Score=254, Evalue=1e-67,
Organism=Caenorhabditis elegans, GI71985574, Length=308, Percent_Identity=28.2467532467532, Blast_Score=88, Evalue=1e-17,
Organism=Saccharomyces cerevisiae, GI6323056, Length=473, Percent_Identity=34.2494714587738, Blast_Score=245, Evalue=2e-65,
Organism=Saccharomyces cerevisiae, GI6323768, Length=473, Percent_Identity=33.8266384778013, Blast_Score=237, Evalue=4e-63,
Organism=Drosophila melanogaster, GI24649832, Length=479, Percent_Identity=33.8204592901879, Blast_Score=220, Evalue=2e-57,

Paralogues:

None

Copy number: 160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 640 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): PUR9_PROM5 (A2BUP7)

Other databases:

- EMBL:   CP000552
- RefSeq:   YP_001010615.1
- ProteinModelPortal:   A2BUP7
- SMR:   A2BUP7
- STRING:   A2BUP7
- GeneID:   4720172
- GenomeReviews:   CP000552_GR
- KEGG:   pmc:P9515_02991
- eggNOG:   COG0138
- HOGENOM:   HBG498048
- OMA:   ASDGFFP
- ProtClustDB:   PRK00881
- BioCyc:   PMAR167542:P9515ORF_0311-MONOMER
- HAMAP:   MF_00139
- InterPro:   IPR002695
- InterPro:   IPR013982
- InterPro:   IPR016193
- InterPro:   IPR011607
- Gene3D:   G3DSA:3.40.50.1380
- PANTHER:   PTHR11692
- PIRSF:   PIRSF000414
- SMART:   SM00798
- SMART:   SM00851
- TIGRFAMs:   TIGR00355

Pfam domain/function: PF01808 AICARFT_IMPCHas; PF02142 MGS; SSF53927 Cytidine_deaminase-like; SSF52335 MGS-like_dom

EC number: =2.1.2.3; =3.5.4.10

Molecular weight: Translated: 57437; Mature: 57306

Theoretical pI: Translated: 6.45; Mature: 6.45

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSPLALVSVSDKTNIIPFCKDLVEKFGYNILSSGGTAEYLTEAKVPVLKVADFTESPEIL
CCCEEEEEECCCCCCCHHHHHHHHHCCCCEECCCCCHHHHHHCCCCEEEECCCCCCCHHH
DGRVKTLHPKIHGGILAKRSNEEHQREILENKLELIDLVVVNLYPFKKKVEEQCPWEEAI
CCCHHEECCHHHCCEEECCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHCCCHHHHH
ENIDIGGPSMIRSAAKNHADVAVLVDPNQYQNYIEEIKKGPLSKDFKTKLAFEAFQHTAS
HCCCCCCHHHHHHHHCCCCCEEEEECCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHH
YDSAISNWISKEKDLRPSNFIESYPLIKQLRYGENPHQKALWYGLNNIGWNSAEQLQGKE
HHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCCCHHHHHHCCCCCCCCCHHHHCCCC
LSYNNILDLESALLTVLEFGYETKPNIKTESIAAVILKHNNPCGASISNSASSSFKNALK
CCCCCHHHHHHHHHHHHHHCCCCCCCCCHHCEEEEEEECCCCCCCCCCCHHHHHHHHHHC
CDSVSAFGGIVAFNANVDKETALILKDIFLECVVAPSFDKEALEIFKTKKNLRVLKLTKE
CCCCHHCCCEEEEECCCCHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCCCEEHHHH
MLPKENQTCSKSIMGGILIQDSDNQENSEDSWISVTKKNPTEQEYLDLKFAWKICKHVKS
HCCCCCCHHHHHHHCCEEEECCCCCCCCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHCC
NAIVVAKDQQTLGIGAGQMNRVGASKIALEAAKEIDSGGVLASDGFFPFADTVRLADKYG
CEEEEEECCCEEECCCCCCCCCCHHHHHHHHHHHCCCCCEEECCCCCCCHHHHHHHHHHC
ISSIIQPGGSIRDEESIKMCDSRGISMIFTHKRHFLH
HHHHHCCCCCCCCCHHHHHHHCCCCEEEEECCHHCCC
>Mature Secondary Structure 
SPLALVSVSDKTNIIPFCKDLVEKFGYNILSSGGTAEYLTEAKVPVLKVADFTESPEIL
CCEEEEEECCCCCCCHHHHHHHHHCCCCEECCCCCHHHHHHCCCCEEEECCCCCCCHHH
DGRVKTLHPKIHGGILAKRSNEEHQREILENKLELIDLVVVNLYPFKKKVEEQCPWEEAI
CCCHHEECCHHHCCEEECCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHCCCHHHHH
ENIDIGGPSMIRSAAKNHADVAVLVDPNQYQNYIEEIKKGPLSKDFKTKLAFEAFQHTAS
HCCCCCCHHHHHHHHCCCCCEEEEECCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHH
YDSAISNWISKEKDLRPSNFIESYPLIKQLRYGENPHQKALWYGLNNIGWNSAEQLQGKE
HHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCCCHHHHHHCCCCCCCCCHHHHCCCC
LSYNNILDLESALLTVLEFGYETKPNIKTESIAAVILKHNNPCGASISNSASSSFKNALK
CCCCCHHHHHHHHHHHHHHCCCCCCCCCHHCEEEEEEECCCCCCCCCCCHHHHHHHHHHC
CDSVSAFGGIVAFNANVDKETALILKDIFLECVVAPSFDKEALEIFKTKKNLRVLKLTKE
CCCCHHCCCEEEEECCCCHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCCCEEHHHH
MLPKENQTCSKSIMGGILIQDSDNQENSEDSWISVTKKNPTEQEYLDLKFAWKICKHVKS
HCCCCCCHHHHHHHCCEEEECCCCCCCCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHCC
NAIVVAKDQQTLGIGAGQMNRVGASKIALEAAKEIDSGGVLASDGFFPFADTVRLADKYG
CEEEEEECCCEEECCCCCCCCCCHHHHHHHHHHHCCCCCEEECCCCCCCHHHHHHHHHHC
ISSIIQPGGSIRDEESIKMCDSRGISMIFTHKRHFLH
HHHHHCCCCCCCCCHHHHHHHCCCCEEEEECCHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA