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The map label for this gene is leuC

Identifier: 123965524

GI number: 123965524

Start: 264637

End: 266046

Strand: Reverse

Name: leuC

Synonym: P9515_02891

Alternate gene names: 123965524

Gene position: 266046-264637 (Counterclockwise)

Preceding gene: 123965525

Following gene: 123965523

Centisome position: 15.61

GC content: 36.88

Gene sequence:

>1410_bases
TTGAGTCAAGATACCTTATTTGATAAAGTCTGGGAATTACACAAAGTTGCAAATCTTCCTGGTGGCTCGGATCAAATCTT
TATCGGTCTTCACCTTATTCATGAAGTTACTAGCCCCCAAGCGTTTGGAGCTTTAAAAGATAAAAATTTAAAAGTCAAAT
TTCCAAATAGGACTGTTGCAACAGTTGATCATATCGTCCCAACAGATAATCAAAACAGGCCTTTTAGAGATAATCTGGCT
GAACAAATGATTGATACTCTAGAAAAGAATTGTTTCGAACACAAAATTAAATTTTTTAATATTGGTAGTGGTAAGCAAGG
AATTGTACATGTAGTAGCTCCAGAACTTGGATTAACACAGCCAGGCATGACTATAGCCTGTGGAGATTCTCATACATCAA
CACATGGAGCCTTTGGATCAATAGCTTTTGGCATTGGTACTAGTCAAGTAAGAGATGTCCTTGCTAGTCAAACGATTGCT
ATGAATAAGCTAAAAGTTAGGCAAATTTGGTGTGAGAATAAATTATCCAATGGAATTTATGCTAAAGATTTAGTACTACA
TATAATTAATCATCTTGGAGTAAAAGCTGGAGTAGGTTATGCCTATGAATTTGCGGGCCCAGCTATAAGTGCATTATCAA
TGGAAGAGAGGATGACAATATGCAATATGTCTATTGAAGGAGGAGCAAGATGCGGTTATATAAATCCTGATGAAAAAACC
TTTAGTTACATCAAGGATAAATTATGCTCGCCTCAAAATGAAAATTGGGATAAGGCTATTAAGTGGTGGAAATCATTACA
AAGTAATGAGAATTCTATTTTTGATGACGTTATCAAAATAGATGCCTCAAGAGTGGAACCTACTGTTACATGGGGTATTA
CTCCTGGACAAAGTATAGGCATTAGCCAGAAAATCCCTTCATTGGGAGAAATACATCCAAATGATCAGTTTATTGCTGGG
GAGGCTTATGAATATATGGGTTTCAAACCTGGACAACCAATAAAAGATACTCCTATCGATGTTTGTTTTATAGGTAGTTG
CACTAATGGAAGAATCAGTGATCTAAGAGTTGCTGCTCGAGTATTAGGGAATCATAAAATAGCAAAAAATATCAAAGCTT
TTGTGGTTCCAGGATCAGAGAGGGTTGCAAAAGAAGCGAAAGAGGAGGGTCTTGATAAAGTTTTCATAGATGCAGGTTTT
CAGTGGAGAGAGCCAGGATGTTCGATGTGTTTAGCAATGAATTCTGACAAATTAATAGGTAACCAAGTAAGTGCAAGCTC
GAGCAATAGAAATTTCAAAGGGAGACAAGGATCTCCGAGCGGTAGAACGCTTTTAATGAGCCCTGCGATGGTAGCTGCTG
CTGCTATAAAGGGAAAAGTAAGTGATGTTAGAGATTTTCTAAATAAATGA

Upstream 100 bases:

>100_bases
CACTAGAAATAGAGTTGAAATTCAAAGACTAAGTGTAAATGTGTGTTTAAACAGTCTTCGATTAATCTTATTATCTAAAA
GTAAGTAACTTTTTTTCAAA

Downstream 100 bases:

>100_bases
ACGTTAAATTCACCCCCCCTATAGGTAGATTTTCACAATTAAATGGTAAATGCATCTCATTAGTTGGAGATGATATTGAT
ACTGATAGAATAATACCCGC

Product: isopropylmalate isomerase large subunit

Products: NA

Alternate protein names: Alpha-IPM isomerase; IPMI; Isopropylmalate isomerase

Number of amino acids: Translated: 469; Mature: 468

Protein sequence:

>469_residues
MSQDTLFDKVWELHKVANLPGGSDQIFIGLHLIHEVTSPQAFGALKDKNLKVKFPNRTVATVDHIVPTDNQNRPFRDNLA
EQMIDTLEKNCFEHKIKFFNIGSGKQGIVHVVAPELGLTQPGMTIACGDSHTSTHGAFGSIAFGIGTSQVRDVLASQTIA
MNKLKVRQIWCENKLSNGIYAKDLVLHIINHLGVKAGVGYAYEFAGPAISALSMEERMTICNMSIEGGARCGYINPDEKT
FSYIKDKLCSPQNENWDKAIKWWKSLQSNENSIFDDVIKIDASRVEPTVTWGITPGQSIGISQKIPSLGEIHPNDQFIAG
EAYEYMGFKPGQPIKDTPIDVCFIGSCTNGRISDLRVAARVLGNHKIAKNIKAFVVPGSERVAKEAKEEGLDKVFIDAGF
QWREPGCSMCLAMNSDKLIGNQVSASSSNRNFKGRQGSPSGRTLLMSPAMVAAAAIKGKVSDVRDFLNK

Sequences:

>Translated_469_residues
MSQDTLFDKVWELHKVANLPGGSDQIFIGLHLIHEVTSPQAFGALKDKNLKVKFPNRTVATVDHIVPTDNQNRPFRDNLA
EQMIDTLEKNCFEHKIKFFNIGSGKQGIVHVVAPELGLTQPGMTIACGDSHTSTHGAFGSIAFGIGTSQVRDVLASQTIA
MNKLKVRQIWCENKLSNGIYAKDLVLHIINHLGVKAGVGYAYEFAGPAISALSMEERMTICNMSIEGGARCGYINPDEKT
FSYIKDKLCSPQNENWDKAIKWWKSLQSNENSIFDDVIKIDASRVEPTVTWGITPGQSIGISQKIPSLGEIHPNDQFIAG
EAYEYMGFKPGQPIKDTPIDVCFIGSCTNGRISDLRVAARVLGNHKIAKNIKAFVVPGSERVAKEAKEEGLDKVFIDAGF
QWREPGCSMCLAMNSDKLIGNQVSASSSNRNFKGRQGSPSGRTLLMSPAMVAAAAIKGKVSDVRDFLNK
>Mature_468_residues
SQDTLFDKVWELHKVANLPGGSDQIFIGLHLIHEVTSPQAFGALKDKNLKVKFPNRTVATVDHIVPTDNQNRPFRDNLAE
QMIDTLEKNCFEHKIKFFNIGSGKQGIVHVVAPELGLTQPGMTIACGDSHTSTHGAFGSIAFGIGTSQVRDVLASQTIAM
NKLKVRQIWCENKLSNGIYAKDLVLHIINHLGVKAGVGYAYEFAGPAISALSMEERMTICNMSIEGGARCGYINPDEKTF
SYIKDKLCSPQNENWDKAIKWWKSLQSNENSIFDDVIKIDASRVEPTVTWGITPGQSIGISQKIPSLGEIHPNDQFIAGE
AYEYMGFKPGQPIKDTPIDVCFIGSCTNGRISDLRVAARVLGNHKIAKNIKAFVVPGSERVAKEAKEEGLDKVFIDAGFQ
WREPGCSMCLAMNSDKLIGNQVSASSSNRNFKGRQGSPSGRTLLMSPAMVAAAAIKGKVSDVRDFLNK

Specific function: Catalyzes the isomerization between 2-isopropylmalate and 3-isopropylmalate, via the formation of 2-isopropylmaleate

COG id: COG0065

COG function: function code E; 3-isopropylmalate dehydratase large subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the aconitase/IPM isomerase family. LeuC type 1 subfamily

Homologues:

Organism=Homo sapiens, GI4501867, Length=400, Percent_Identity=25.5, Blast_Score=96, Evalue=1e-19,
Organism=Homo sapiens, GI8659555, Length=459, Percent_Identity=24.1830065359477, Blast_Score=92, Evalue=9e-19,
Organism=Homo sapiens, GI41352693, Length=395, Percent_Identity=25.0632911392405, Blast_Score=83, Evalue=5e-16,
Organism=Escherichia coli, GI1786259, Length=463, Percent_Identity=48.5961123110151, Blast_Score=450, Evalue=1e-128,
Organism=Escherichia coli, GI1787531, Length=445, Percent_Identity=24.9438202247191, Blast_Score=96, Evalue=4e-21,
Organism=Caenorhabditis elegans, GI32564738, Length=409, Percent_Identity=27.6283618581907, Blast_Score=120, Evalue=1e-27,
Organism=Caenorhabditis elegans, GI25149337, Length=409, Percent_Identity=27.6283618581907, Blast_Score=120, Evalue=1e-27,
Organism=Caenorhabditis elegans, GI25149342, Length=317, Percent_Identity=28.7066246056782, Blast_Score=108, Evalue=5e-24,
Organism=Caenorhabditis elegans, GI17568399, Length=460, Percent_Identity=25.2173913043478, Blast_Score=90, Evalue=2e-18,
Organism=Saccharomyces cerevisiae, GI6321429, Length=473, Percent_Identity=50.3171247357294, Blast_Score=439, Evalue=1e-124,
Organism=Saccharomyces cerevisiae, GI6322261, Length=408, Percent_Identity=27.9411764705882, Blast_Score=131, Evalue=2e-31,
Organism=Saccharomyces cerevisiae, GI6320440, Length=451, Percent_Identity=26.6075388026608, Blast_Score=124, Evalue=3e-29,
Organism=Saccharomyces cerevisiae, GI6323335, Length=393, Percent_Identity=25.6997455470738, Blast_Score=114, Evalue=2e-26,
Organism=Drosophila melanogaster, GI161076999, Length=391, Percent_Identity=27.8772378516624, Blast_Score=112, Evalue=7e-25,
Organism=Drosophila melanogaster, GI281365315, Length=391, Percent_Identity=27.8772378516624, Blast_Score=112, Evalue=7e-25,
Organism=Drosophila melanogaster, GI17864292, Length=391, Percent_Identity=27.8772378516624, Blast_Score=112, Evalue=7e-25,
Organism=Drosophila melanogaster, GI28571643, Length=390, Percent_Identity=27.6923076923077, Blast_Score=110, Evalue=2e-24,
Organism=Drosophila melanogaster, GI24645686, Length=373, Percent_Identity=28.686327077748, Blast_Score=97, Evalue=2e-20,
Organism=Drosophila melanogaster, GI17137564, Length=370, Percent_Identity=27.5675675675676, Blast_Score=94, Evalue=1e-19,

Paralogues:

None

Copy number: 280 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): LEUC_PROM5 (A2BUN7)

Other databases:

- EMBL:   CP000552
- RefSeq:   YP_001010605.1
- ProteinModelPortal:   A2BUN7
- SMR:   A2BUN7
- STRING:   A2BUN7
- GeneID:   4720161
- GenomeReviews:   CP000552_GR
- KEGG:   pmc:P9515_02891
- eggNOG:   COG0065
- HOGENOM:   HBG330745
- OMA:   RPHAPKG
- ProtClustDB:   PRK05478
- BioCyc:   PMAR167542:P9515ORF_0300-MONOMER
- HAMAP:   MF_01026
- InterPro:   IPR004430
- InterPro:   IPR015931
- InterPro:   IPR015937
- InterPro:   IPR001030
- InterPro:   IPR015932
- InterPro:   IPR018136
- InterPro:   IPR015936
- Gene3D:   G3DSA:3.30.499.10
- Gene3D:   G3DSA:3.40.1060.10
- PANTHER:   PTHR11670
- PANTHER:   PTHR11670:SF6
- PRINTS:   PR00415
- TIGRFAMs:   TIGR00170

Pfam domain/function: PF00330 Aconitase; SSF53732 Aconitase_N

EC number: =4.2.1.33

Molecular weight: Translated: 51342; Mature: 51211

Theoretical pI: Translated: 8.27; Mature: 8.27

Prosite motif: PS00450 ACONITASE_1; PS01244 ACONITASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSQDTLFDKVWELHKVANLPGGSDQIFIGLHLIHEVTSPQAFGALKDKNLKVKFPNRTVA
CCCCHHHHHHHHHHHHHCCCCCCCEEEEHHHHHHHHCCCHHHCCCCCCCEEEECCCCEEE
TVDHIVPTDNQNRPFRDNLAEQMIDTLEKNCFEHKIKFFNIGSGKQGIVHVVAPELGLTQ
EHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCEEEEEECCCCCCEEEEEECCCCCCC
PGMTIACGDSHTSTHGAFGSIAFGIGTSQVRDVLASQTIAMNKLKVRQIWCENKLSNGIY
CCCEEEECCCCCCCCCCCCEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCH
AKDLVLHIINHLGVKAGVGYAYEFAGPAISALSMEERMTICNMSIEGGARCGYINPDEKT
HHHHHHHHHHHCCCCCCCCEEEECCCCHHHHHHHHHHHEEEEEEECCCCCCCCCCCCHHH
FSYIKDKLCSPQNENWDKAIKWWKSLQSNENSIFDDVIKIDASRVEPTVTWGITPGQSIG
HHHHHHHHCCCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCEEEECCCCCCCCC
ISQKIPSLGEIHPNDQFIAGEAYEYMGFKPGQPIKDTPIDVCFIGSCTNGRISDLRVAAR
CHHCCCCCCCCCCCCCEEECCHHHHCCCCCCCCCCCCCCCEEEEECCCCCCHHHHHHHHH
VLGNHKIAKNIKAFVVPGSERVAKEAKEEGLDKVFIDAGFQWREPGCSMCLAMNSDKLIG
HHCCHHHHCCCEEEEECCHHHHHHHHHHCCCCEEEEECCCEECCCCCCEEEEECCCCCCC
NQVSASSSNRNFKGRQGSPSGRTLLMSPAMVAAAAIKGKVSDVRDFLNK
CCCCCCCCCCCCCCCCCCCCCCEEEECHHHHHHHHHHCCHHHHHHHHCC
>Mature Secondary Structure 
SQDTLFDKVWELHKVANLPGGSDQIFIGLHLIHEVTSPQAFGALKDKNLKVKFPNRTVA
CCCHHHHHHHHHHHHHCCCCCCCEEEEHHHHHHHHCCCHHHCCCCCCCEEEECCCCEEE
TVDHIVPTDNQNRPFRDNLAEQMIDTLEKNCFEHKIKFFNIGSGKQGIVHVVAPELGLTQ
EHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCEEEEEECCCCCCEEEEEECCCCCCC
PGMTIACGDSHTSTHGAFGSIAFGIGTSQVRDVLASQTIAMNKLKVRQIWCENKLSNGIY
CCCEEEECCCCCCCCCCCCEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCH
AKDLVLHIINHLGVKAGVGYAYEFAGPAISALSMEERMTICNMSIEGGARCGYINPDEKT
HHHHHHHHHHHCCCCCCCCEEEECCCCHHHHHHHHHHHEEEEEEECCCCCCCCCCCCHHH
FSYIKDKLCSPQNENWDKAIKWWKSLQSNENSIFDDVIKIDASRVEPTVTWGITPGQSIG
HHHHHHHHCCCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCEEEECCCCCCCCC
ISQKIPSLGEIHPNDQFIAGEAYEYMGFKPGQPIKDTPIDVCFIGSCTNGRISDLRVAAR
CHHCCCCCCCCCCCCCEEECCHHHHCCCCCCCCCCCCCCCEEEEECCCCCCHHHHHHHHH
VLGNHKIAKNIKAFVVPGSERVAKEAKEEGLDKVFIDAGFQWREPGCSMCLAMNSDKLIG
HHCCHHHHCCCEEEEECCHHHHHHHHHHCCCCEEEEECCCEECCCCCCEEEEECCCCCCC
NQVSASSSNRNFKGRQGSPSGRTLLMSPAMVAAAAIKGKVSDVRDFLNK
CCCCCCCCCCCCCCCCCCCCCCEEEECHHHHHHHHHHCCHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA