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The map label for this gene is leuD [H]

Identifier: 123965523

GI number: 123965523

Start: 264020

End: 264640

Strand: Reverse

Name: leuD [H]

Synonym: P9515_02881

Alternate gene names: 123965523

Gene position: 264640-264020 (Counterclockwise)

Preceding gene: 123965524

Following gene: 123965519

Centisome position: 15.53

GC content: 29.95

Gene sequence:

>621_bases
ATGAACGTTAAATTCACCCCCCCTATAGGTAGATTTTCACAATTAAATGGTAAATGCATCTCATTAGTTGGAGATGATAT
TGATACTGATAGAATAATACCCGCTCGTTTTTTAAAATGTGTAAATTTTGATTCCTTAGGAAAATCTGTTTTTGATGACG
ATCGCAAAGCTCTTAAAGGAAAACATCCTTTTGATTTAGAGGCTAATAAAGGTGCCTCGATACTCATTGTTAACAGTAAT
TTTGGATGTGGTTCAAGCAGAGAACATGCTCCCCAAGCATTAATGAGATGGGGCATTAAGGCAATCATTGGCGAAAGTTT
CGCGGATATTTTTTATAGCAATTGCATTGCGATTGGTGTTCCATGTTTTACTCTTTCTAAGAAATCAATACAAATTATCC
AAAAATATATCAATAATAAAGCTTTATTTTTTGAGGTTGACCTTTTAAAATCAGTTGCATTATCAAAAGATTTAAAATTT
GATCTTGAAATAAAAGAAACATCAAGAAAAATGTTTTTATCGGGCGAATGGGATACCACATCAAAATTAATTGAAAATAA
TGATTTAATTGAAAAAAAATTGAATGAATTACCTTATATTAAATTCAATTCTAATTTCTAA

Upstream 100 bases:

>100_bases
AAGGATCTCCGAGCGGTAGAACGCTTTTAATGAGCCCTGCGATGGTAGCTGCTGCTGCTATAAAGGGAAAAGTAAGTGAT
GTTAGAGATTTTCTAAATAA

Downstream 100 bases:

>100_bases
AACCTATTGGAAGCCAAATAAAGTAAAAGCTATACCCCCTGCTTCATTATGCGCTTGATAAAAGATGCCAAATTCATAGG
ATCTTTTTTTCCAATTTAAC

Product: 3-isopropylmalate dehydratase small subunit

Products: NA

Alternate protein names: Alpha-IPM isomerase; IPMI; Isopropylmalate isomerase [H]

Number of amino acids: Translated: 206; Mature: 206

Protein sequence:

>206_residues
MNVKFTPPIGRFSQLNGKCISLVGDDIDTDRIIPARFLKCVNFDSLGKSVFDDDRKALKGKHPFDLEANKGASILIVNSN
FGCGSSREHAPQALMRWGIKAIIGESFADIFYSNCIAIGVPCFTLSKKSIQIIQKYINNKALFFEVDLLKSVALSKDLKF
DLEIKETSRKMFLSGEWDTTSKLIENNDLIEKKLNELPYIKFNSNF

Sequences:

>Translated_206_residues
MNVKFTPPIGRFSQLNGKCISLVGDDIDTDRIIPARFLKCVNFDSLGKSVFDDDRKALKGKHPFDLEANKGASILIVNSN
FGCGSSREHAPQALMRWGIKAIIGESFADIFYSNCIAIGVPCFTLSKKSIQIIQKYINNKALFFEVDLLKSVALSKDLKF
DLEIKETSRKMFLSGEWDTTSKLIENNDLIEKKLNELPYIKFNSNF
>Mature_206_residues
MNVKFTPPIGRFSQLNGKCISLVGDDIDTDRIIPARFLKCVNFDSLGKSVFDDDRKALKGKHPFDLEANKGASILIVNSN
FGCGSSREHAPQALMRWGIKAIIGESFADIFYSNCIAIGVPCFTLSKKSIQIIQKYINNKALFFEVDLLKSVALSKDLKF
DLEIKETSRKMFLSGEWDTTSKLIENNDLIEKKLNELPYIKFNSNF

Specific function: Catalyzes the isomerization between 2-isopropylmalate and 3-isopropylmalate, via the formation of 2-isopropylmaleate [H]

COG id: COG0066

COG function: function code E; 3-isopropylmalate dehydratase small subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the leuD family. LeuD type 1 subfamily [H]

Homologues:

Organism=Escherichia coli, GI1786258, Length=187, Percent_Identity=33.1550802139037, Blast_Score=96, Evalue=1e-21,
Organism=Saccharomyces cerevisiae, GI6321429, Length=154, Percent_Identity=38.961038961039, Blast_Score=84, Evalue=2e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004431
- InterPro:   IPR012305
- InterPro:   IPR015937
- InterPro:   IPR015928
- InterPro:   IPR000573 [H]

Pfam domain/function: PF00694 Aconitase_C [H]

EC number: =4.2.1.33 [H]

Molecular weight: Translated: 23212; Mature: 23212

Theoretical pI: Translated: 8.72; Mature: 8.72

Prosite motif: PS00094 C5_MTASE_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.4 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
2.4 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNVKFTPPIGRFSQLNGKCISLVGDDIDTDRIIPARFLKCVNFDSLGKSVFDDDRKALKG
CCCEECCCCCCHHHCCCEEEEEECCCCCCCCCCHHHHHHHCCHHHHHHHHHHCHHHHHCC
KHPFDLEANKGASILIVNSNFGCGSSREHAPQALMRWGIKAIIGESFADIFYSNCIAIGV
CCCCCCCCCCCCEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCHHHCCC
PCFTLSKKSIQIIQKYINNKALFFEVDLLKSVALSKDLKFDLEIKETSRKMFLSGEWDTT
CCEECCHHHHHHHHHHHCCCEEEEEHHHHHHHHHCCCCEEEEEEECCCCEEEEECCCCHH
SKLIENNDLIEKKLNELPYIKFNSNF
HHHHCCCHHHHHHHHHCCEEEECCCC
>Mature Secondary Structure
MNVKFTPPIGRFSQLNGKCISLVGDDIDTDRIIPARFLKCVNFDSLGKSVFDDDRKALKG
CCCEECCCCCCHHHCCCEEEEEECCCCCCCCCCHHHHHHHCCHHHHHHHHHHCHHHHHCC
KHPFDLEANKGASILIVNSNFGCGSSREHAPQALMRWGIKAIIGESFADIFYSNCIAIGV
CCCCCCCCCCCCEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCHHHCCC
PCFTLSKKSIQIIQKYINNKALFFEVDLLKSVALSKDLKFDLEIKETSRKMFLSGEWDTT
CCEECCHHHHHHHHHHHCCCEEEEEHHHHHHHHHCCCCEEEEEEECCCCEEEEECCCCHH
SKLIENNDLIEKKLNELPYIKFNSNF
HHHHCCCHHHHHHHHHCCEEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8905231 [H]