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The map label for this gene is echA20 [H]

Identifier: 121639469

GI number: 121639469

Start: 3968045

End: 3968788

Strand: Direct

Name: echA20 [H]

Synonym: BCG_3614

Alternate gene names: 121639469

Gene position: 3968045-3968788 (Clockwise)

Preceding gene: 121639466

Following gene: 121639470

Centisome position: 90.71

GC content: 68.15

Gene sequence:

>744_bases
ATGCCGATCACCTCCACCACGCCCGAACCGGGCATCGTCGCGGTCACCGTCGACTACCCGCCGGTCAACGCCATCCCGTC
GAAAGCGTGGTTCGACCTGGCCGACGCGGTGACGGCCGCGGGCGCCAACTCCGACACCCGCGCGGTGATCCTGCGGGCCG
AGGGGCGCGGCTTCAACGCCGGGGTGGACATCAAAGAGATGCAACGAACCGAAGGTTTCACGGCGCTGATCGACGCCAAC
CGCGGCTGCTTCGCCGCATTCCGCGCCGTCTACGAGTGCGCGGTGCCGGTGATCGCCGCCGTGAACGGATTCTGCGTGGG
CGGCGGCATCGGCCTGGTCGGCAACTCCGACGTCATCGTGGCCTCCGAGGACGCCACCTTCGGCCTGCCCGAGGTGGAAC
GGGGCGCGCTGGGCGCGGCCACGCACCTCTCGCGGCTGGTGCCCCAGCACCTGATGCGACGGCTGTTCTTTACGGCGGCC
ACCGTGGACGCGGCCACCTTGCAGCACTTCGGCTCGGTGCACGAGGTGGTGTCCCGCGATCAGCTGGACGAGGCCGCTTT
GCGGGTGGCCCGCGACATCGCCGCCAAAGACACCCGGGTCATCCGCGCCGCCAAGGAGGCGCTGAACTTCATCGACGTGC
AACGGGTCAATGCGAGTTACCGGATGGAGCAAGGTTTTACCTTCGAGCTCAACCTCGCCGGAGTCGCCGACGAGCACCGC
GACGCCTTTGTGAAGAAGTCATAG

Upstream 100 bases:

>100_bases
CGGCCAGCCCGAAATTGATGGCGTCGGCTGCCTCGGCGAGAGTCACTGTGCTAGTGTACCAAGCAAGTGCTTGCTTAGGT
AGCGAACCCGCAGGAGTGCA

Downstream 100 bases:

>100_bases
TGCCCGATAAACGAACCTCTCTTGACGACGCCGTCGCGCAATTGCGCAGCGGCATGACCATCGGCATCGCCGGCTGGGGC
TCGCGGCGCAAGCCCATGGC

Product: enoyl-CoA hydratase

Products: trans-2-enoyl-CoA; trans-3-enoyl-CoA; H2O

Alternate protein names: Crotonobetainyl-CoA hydratase [H]

Number of amino acids: Translated: 247; Mature: 246

Protein sequence:

>247_residues
MPITSTTPEPGIVAVTVDYPPVNAIPSKAWFDLADAVTAAGANSDTRAVILRAEGRGFNAGVDIKEMQRTEGFTALIDAN
RGCFAAFRAVYECAVPVIAAVNGFCVGGGIGLVGNSDVIVASEDATFGLPEVERGALGAATHLSRLVPQHLMRRLFFTAA
TVDAATLQHFGSVHEVVSRDQLDEAALRVARDIAAKDTRVIRAAKEALNFIDVQRVNASYRMEQGFTFELNLAGVADEHR
DAFVKKS

Sequences:

>Translated_247_residues
MPITSTTPEPGIVAVTVDYPPVNAIPSKAWFDLADAVTAAGANSDTRAVILRAEGRGFNAGVDIKEMQRTEGFTALIDAN
RGCFAAFRAVYECAVPVIAAVNGFCVGGGIGLVGNSDVIVASEDATFGLPEVERGALGAATHLSRLVPQHLMRRLFFTAA
TVDAATLQHFGSVHEVVSRDQLDEAALRVARDIAAKDTRVIRAAKEALNFIDVQRVNASYRMEQGFTFELNLAGVADEHR
DAFVKKS
>Mature_246_residues
PITSTTPEPGIVAVTVDYPPVNAIPSKAWFDLADAVTAAGANSDTRAVILRAEGRGFNAGVDIKEMQRTEGFTALIDANR
GCFAAFRAVYECAVPVIAAVNGFCVGGGIGLVGNSDVIVASEDATFGLPEVERGALGAATHLSRLVPQHLMRRLFFTAAT
VDAATLQHFGSVHEVVSRDQLDEAALRVARDIAAKDTRVIRAAKEALNFIDVQRVNASYRMEQGFTFELNLAGVADEHRD
AFVKKS

Specific function: Catalyzes the reversible dehydration of L-carnitinyl-CoA to crotonobetainyl-CoA [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the enoyl-CoA hydratase/isomerase family [H]

Homologues:

Organism=Homo sapiens, GI31542718, Length=165, Percent_Identity=30.3030303030303, Blast_Score=82, Evalue=3e-16,
Organism=Homo sapiens, GI194097323, Length=207, Percent_Identity=28.5024154589372, Blast_Score=79, Evalue=3e-15,
Organism=Homo sapiens, GI70995211, Length=171, Percent_Identity=29.2397660818713, Blast_Score=69, Evalue=3e-12,
Organism=Escherichia coli, GI1787659, Length=242, Percent_Identity=30.1652892561983, Blast_Score=92, Evalue=5e-20,
Organism=Escherichia coli, GI221142681, Length=214, Percent_Identity=29.9065420560748, Blast_Score=90, Evalue=2e-19,
Organism=Escherichia coli, GI1788597, Length=205, Percent_Identity=26.8292682926829, Blast_Score=65, Evalue=5e-12,
Organism=Escherichia coli, GI87082183, Length=192, Percent_Identity=27.6041666666667, Blast_Score=63, Evalue=2e-11,
Organism=Escherichia coli, GI1790281, Length=221, Percent_Identity=26.2443438914027, Blast_Score=60, Evalue=9e-11,
Organism=Caenorhabditis elegans, GI17554946, Length=239, Percent_Identity=29.2887029288703, Blast_Score=92, Evalue=4e-19,
Organism=Caenorhabditis elegans, GI17534483, Length=246, Percent_Identity=27.2357723577236, Blast_Score=88, Evalue=5e-18,
Organism=Caenorhabditis elegans, GI25145438, Length=228, Percent_Identity=26.3157894736842, Blast_Score=82, Evalue=2e-16,
Organism=Caenorhabditis elegans, GI17536985, Length=230, Percent_Identity=26.0869565217391, Blast_Score=76, Evalue=1e-14,
Organism=Caenorhabditis elegans, GI17540714, Length=224, Percent_Identity=27.6785714285714, Blast_Score=72, Evalue=3e-13,
Organism=Caenorhabditis elegans, GI17540306, Length=184, Percent_Identity=28.2608695652174, Blast_Score=68, Evalue=4e-12,
Organism=Drosophila melanogaster, GI19920382, Length=222, Percent_Identity=30.1801801801802, Blast_Score=94, Evalue=7e-20,
Organism=Drosophila melanogaster, GI20129971, Length=221, Percent_Identity=28.0542986425339, Blast_Score=86, Evalue=3e-17,
Organism=Drosophila melanogaster, GI24653477, Length=221, Percent_Identity=28.0542986425339, Blast_Score=86, Evalue=3e-17,
Organism=Drosophila melanogaster, GI24653139, Length=210, Percent_Identity=28.0952380952381, Blast_Score=68, Evalue=5e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR022852
- InterPro:   IPR014748
- InterPro:   IPR001753
- InterPro:   IPR018376 [H]

Pfam domain/function: PF00378 ECH [H]

EC number: 4.2.1.17

Molecular weight: Translated: 26333; Mature: 26202

Theoretical pI: Translated: 5.34; Mature: 5.34

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPITSTTPEPGIVAVTVDYPPVNAIPSKAWFDLADAVTAAGANSDTRAVILRAEGRGFNA
CCCCCCCCCCCEEEEEECCCCCCCCCCHHHHHHHHHHHHCCCCCCCEEEEEEECCCCCCC
GVDIKEMQRTEGFTALIDANRGCFAAFRAVYECAVPVIAAVNGFCVGGGIGLVGNSDVIV
CCCHHHHHHCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHCCEEEECCEEEECCCCEEE
ASEDATFGLPEVERGALGAATHLSRLVPQHLMRRLFFTAATVDAATLQHFGSVHEVVSRD
EECCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
QLDEAALRVARDIAAKDTRVIRAAKEALNFIDVQRVNASYRMEQGFTFELNLAGVADEHR
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEECCCCEEEEEECCCCCHHH
DAFVKKS
HHHCCCC
>Mature Secondary Structure 
PITSTTPEPGIVAVTVDYPPVNAIPSKAWFDLADAVTAAGANSDTRAVILRAEGRGFNA
CCCCCCCCCCEEEEEECCCCCCCCCCHHHHHHHHHHHHCCCCCCCEEEEEEECCCCCCC
GVDIKEMQRTEGFTALIDANRGCFAAFRAVYECAVPVIAAVNGFCVGGGIGLVGNSDVIV
CCCHHHHHHCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHCCEEEECCEEEECCCCEEE
ASEDATFGLPEVERGALGAATHLSRLVPQHLMRRLFFTAATVDAATLQHFGSVHEVVSRD
EECCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
QLDEAALRVARDIAAKDTRVIRAAKEALNFIDVQRVNASYRMEQGFTFELNLAGVADEHR
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEECCCCEEEEEECCCCCHHH
DAFVKKS
HHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 0.05 {crotonyl-CoA}} 0.008 {2-decenoyl-CoA}} [C]

Substrates: (3S)-3-hydroxyacyl-CoA

Specific reaction: (3S)-3-hydroxyacyl-CoA = trans-2(or 3)-enoyl-CoA + H2O

General reaction: addition (of H2O to carbon carbon double bondelimination (of H2O C-O bond cleavagehydration [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA