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The map label for this gene is ddn

Identifier: 121639466

GI number: 121639466

Start: 3965736

End: 3966191

Strand: Direct

Name: ddn

Synonym: BCG_3611

Alternate gene names: 121639466

Gene position: 3965736-3966191 (Clockwise)

Preceding gene: 121639465

Following gene: 121639469

Centisome position: 90.66

GC content: 60.96

Gene sequence:

>456_bases
ATGCCGAAATCACCGCCGCGGTTTCTGAATTCGCCGCTCAGCGACTTCTTTATCAAGTGGATGTCACGGATTAATACCTG
GATGTACCGCCGCAACGACGGGGAGGGTCTGGGCGGCACCTTCCAGAAGATTCCGGTCGCGCTGCTGACCACCACCGGCC
GCAAGACCGGCCAGCCGCGGGTCAACCCGCTCTACTTCCTGCGCGACGGTGGGCGGGTCATTGTCGCGGCCTCCAAGGGC
GGCGCGGAGAAGAACCCGATGTGGTACCTCAACCTCAAGGCCAACCCCAAGGTTCAGGTACAGATCAAAAAGGAAGTGCT
GGACCTTACCGCGCGGGACGCGACCGACGAGGAGCGCGCCGAATATTGGCCACAGTTGGTCACGATGTACCCAAGTTATC
AGGACTACCAGTCCTGGACCGACCGCACGATCCCGATCGTGGTTTGCGAACCCTGA

Upstream 100 bases:

>100_bases
CAGGGCACCGTGCGGCGTGACTGCAACATGAAGCGACCGATGATTAGATAGCGAGGCGGACGCGCGCCTTTGGCGACCCT
TGGTCGCTAGGATCAGCGTC

Downstream 100 bases:

>100_bases
CCGTTCCCAACTTCGCCGAACGTGAAGCCAGGGCGAGAAAACGGCCGAAATCTCGCCCTGAGTTCACGCTCGGCGCAGAT
AACTAGGCCCCATAGACCGG

Product: hypothetical protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 151; Mature: 150

Protein sequence:

>151_residues
MPKSPPRFLNSPLSDFFIKWMSRINTWMYRRNDGEGLGGTFQKIPVALLTTTGRKTGQPRVNPLYFLRDGGRVIVAASKG
GAEKNPMWYLNLKANPKVQVQIKKEVLDLTARDATDEERAEYWPQLVTMYPSYQDYQSWTDRTIPIVVCEP

Sequences:

>Translated_151_residues
MPKSPPRFLNSPLSDFFIKWMSRINTWMYRRNDGEGLGGTFQKIPVALLTTTGRKTGQPRVNPLYFLRDGGRVIVAASKG
GAEKNPMWYLNLKANPKVQVQIKKEVLDLTARDATDEERAEYWPQLVTMYPSYQDYQSWTDRTIPIVVCEP
>Mature_150_residues
PKSPPRFLNSPLSDFFIKWMSRINTWMYRRNDGEGLGGTFQKIPVALLTTTGRKTGQPRVNPLYFLRDGGRVIVAASKGG
AEKNPMWYLNLKANPKVQVQIKKEVLDLTARDATDEERAEYWPQLVTMYPSYQDYQSWTDRTIPIVVCEP

Specific function: Nitroreductase involved in the bioreductive activation of the antitubercular prodrug PA-824 (nitroimidazo-oxazine) developed for anti-tuberculosis therapy against both replicating and persistent bacteria. It converts PA-824 into three primary metabolites;

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the deazaflavin-dependent nitroreductase family

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): DDN_MYCTU (P71854)

Other databases:

- EMBL:   BX842583
- EMBL:   AE000516
- PIR:   D70677
- RefSeq:   NP_218064.1
- RefSeq:   NP_338196.1
- ProteinModelPortal:   P71854
- EnsemblBacteria:   EBMYCT00000002095
- EnsemblBacteria:   EBMYCT00000071962
- GeneID:   887496
- GeneID:   922850
- GenomeReviews:   AE000516_GR
- GenomeReviews:   AL123456_GR
- KEGG:   mtc:MT3651
- KEGG:   mtu:Rv3547
- TIGR:   MT3651
- TubercuList:   Rv3547
- GeneTree:   EBGT00050000015629
- HOGENOM:   HBG657787
- OMA:   ILTTIGR
- ProtClustDB:   CLSK792555
- InterPro:   IPR012349
- InterPro:   IPR009002
- InterPro:   IPR004378
- Gene3D:   G3DSA:2.30.110.10
- TIGRFAMs:   TIGR00026

Pfam domain/function: PF04075 DUF385; SSF50475 FMN_binding

EC number: NA

Molecular weight: Translated: 17371; Mature: 17240

Theoretical pI: Translated: 9.94; Mature: 9.94

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPKSPPRFLNSPLSDFFIKWMSRINTWMYRRNDGEGLGGTFQKIPVALLTTTGRKTGQPR
CCCCCHHHHCCCHHHHHHHHHHHHHHHEEECCCCCCCCCCHHHCCEEEEEECCCCCCCCC
VNPLYFLRDGGRVIVAASKGGAEKNPMWYLNLKANPKVQVQIKKEVLDLTARDATDEERA
CCEEEEEECCCEEEEEECCCCCCCCCEEEEEECCCCEEEEEEHHHHHHHHCCCCCCHHHH
EYWPQLVTMYPSYQDYQSWTDRTIPIVVCEP
HHHHHHEEECCCCHHHHHHCCCCEEEEEECC
>Mature Secondary Structure 
PKSPPRFLNSPLSDFFIKWMSRINTWMYRRNDGEGLGGTFQKIPVALLTTTGRKTGQPR
CCCCHHHHCCCHHHHHHHHHHHHHHHEEECCCCCCCCCCHHHCCEEEEEECCCCCCCCC
VNPLYFLRDGGRVIVAASKGGAEKNPMWYLNLKANPKVQVQIKKEVLDLTARDATDEERA
CCEEEEEECCCEEEEEECCCCCCCCCEEEEEECCCCEEEEEEHHHHHHHHCCCCCCHHHH
EYWPQLVTMYPSYQDYQSWTDRTIPIVVCEP
HHHHHHEEECCCCHHHHHHCCCCEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9634230; 12218036