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The map label for this gene is echA18 [H]

Identifier: 121639300

GI number: 121639300

Start: 3775290

End: 3776180

Strand: Direct

Name: echA18 [H]

Synonym: BCG_3445

Alternate gene names: 121639300

Gene position: 3775290-3776180 (Clockwise)

Preceding gene: 121639299

Following gene: 121639301

Centisome position: 86.3

GC content: 67.0

Gene sequence:

>891_bases
ATGAGGCGGCGTGCAATGACGAAGATGGACGAGGCTAGCAATCCGTGCGGCGGGGACATCGAAGCTGAGATGTGCCAGTT
GATGCGCGAGCAACCACCCGCCGAAGGCGTCGTCGATCGTGTCGCGCTGCAACGCCATCGAAACGTTGCGTTGATCACGC
TGAGCCATCCGCAGGCGCAGAACGCACTCAACCTGGCGAGCTGGCGTCGGCTGAAGCGGCTGCTGGACGATCTCGCCGGC
GAATCGGGGCTGCGGGCGGTGGTGCTGCGGGGCGCCGGTGACAAGGCGTTCGCCGCGGGTGCCGACATCAAGGAGTTTCC
GAACACCCGCATGAGCGCCGCGGACGCCGCGGAGTACAACGAGAGCCTGGCCGTCTGCCTGAGGGCGTTGACCACGATGC
CGATCCCAGTCATCGCGGCGGTCCGGGGGCTCGCCGTCGGTGGCGGCTGTGAGCTGGCGACGGCCTGCGATGTGTGCATC
GCGACCGACGACGCGCGCTTCGGCATCCCGCTGGGCAAGCTCGGCGTCACGACGGGCTTCACCGAGGCGGACACCGTCGC
GCGCCTCATCGGTCCGGCGGCGCTGAAGTATCTGTTGTTCAGCGGAGAACTGATCGGCATTGAGGAAGCCGCCCGCTGGG
GATTGGTGCAAAAGGTCGTCGCACCACAGGATTTGGCGGCCGCGACGGCCAAACTGGTCGGCCAGGTCTGTCGGCAATCC
GCGGTGACCATGCGTGCGGCGAAGGTGGTCGCCAACATGCACGGCCGAGCGCTGACCGGCGCCGACACCGATGCGCTGAT
CCGGTTCGGTGTCGAAGCCTACGAGGGGGCGGACCTACGCGAAGGGGTGGCGGCCTTCAGCCAGGGACGCCCACCCAAAT
TTGATGATTAG

Upstream 100 bases:

>100_bases
CTATGCGCGTCCTCTGCGGTGACCTGGCTGGTAGCTTAGGCACGTCTTTATCGACACCGGGTGCTGCCAGAGAACTCGAG
ACGCGGCACAGGTCGGCACC

Downstream 100 bases:

>100_bases
CGCCATGACCGATGCTGACAGTGCGGTCCCTCCCCGACTCGACGAGGACGCGATCTCGAAACTCGAGCTGACCGAGGTCG
CCGACCTGATCCGCACCCGG

Product: putative enoyl-CoA hydratase echA18

Products: NA

Alternate protein names: Crotonase [H]

Number of amino acids: Translated: 296; Mature: 296

Protein sequence:

>296_residues
MRRRAMTKMDEASNPCGGDIEAEMCQLMREQPPAEGVVDRVALQRHRNVALITLSHPQAQNALNLASWRRLKRLLDDLAG
ESGLRAVVLRGAGDKAFAAGADIKEFPNTRMSAADAAEYNESLAVCLRALTTMPIPVIAAVRGLAVGGGCELATACDVCI
ATDDARFGIPLGKLGVTTGFTEADTVARLIGPAALKYLLFSGELIGIEEAARWGLVQKVVAPQDLAAATAKLVGQVCRQS
AVTMRAAKVVANMHGRALTGADTDALIRFGVEAYEGADLREGVAAFSQGRPPKFDD

Sequences:

>Translated_296_residues
MRRRAMTKMDEASNPCGGDIEAEMCQLMREQPPAEGVVDRVALQRHRNVALITLSHPQAQNALNLASWRRLKRLLDDLAG
ESGLRAVVLRGAGDKAFAAGADIKEFPNTRMSAADAAEYNESLAVCLRALTTMPIPVIAAVRGLAVGGGCELATACDVCI
ATDDARFGIPLGKLGVTTGFTEADTVARLIGPAALKYLLFSGELIGIEEAARWGLVQKVVAPQDLAAATAKLVGQVCRQS
AVTMRAAKVVANMHGRALTGADTDALIRFGVEAYEGADLREGVAAFSQGRPPKFDD
>Mature_296_residues
MRRRAMTKMDEASNPCGGDIEAEMCQLMREQPPAEGVVDRVALQRHRNVALITLSHPQAQNALNLASWRRLKRLLDDLAG
ESGLRAVVLRGAGDKAFAAGADIKEFPNTRMSAADAAEYNESLAVCLRALTTMPIPVIAAVRGLAVGGGCELATACDVCI
ATDDARFGIPLGKLGVTTGFTEADTVARLIGPAALKYLLFSGELIGIEEAARWGLVQKVVAPQDLAAATAKLVGQVCRQS
AVTMRAAKVVANMHGRALTGADTDALIRFGVEAYEGADLREGVAAFSQGRPPKFDD

Specific function: Could Possibly Oxidizes Fatty Acids Using Specific Components (By Similarity). [C]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the enoyl-CoA hydratase/isomerase family [H]

Homologues:

Organism=Homo sapiens, GI194097323, Length=258, Percent_Identity=31.7829457364341, Blast_Score=110, Evalue=2e-24,
Organism=Homo sapiens, GI31542718, Length=245, Percent_Identity=29.7959183673469, Blast_Score=92, Evalue=5e-19,
Organism=Homo sapiens, GI4502327, Length=261, Percent_Identity=28.735632183908, Blast_Score=87, Evalue=3e-17,
Organism=Homo sapiens, GI70995211, Length=264, Percent_Identity=26.5151515151515, Blast_Score=79, Evalue=4e-15,
Organism=Homo sapiens, GI68989263, Length=176, Percent_Identity=35.7954545454545, Blast_Score=79, Evalue=6e-15,
Organism=Escherichia coli, GI1787659, Length=259, Percent_Identity=33.5907335907336, Blast_Score=111, Evalue=4e-26,
Organism=Escherichia coli, GI1788597, Length=264, Percent_Identity=29.1666666666667, Blast_Score=102, Evalue=3e-23,
Organism=Escherichia coli, GI221142681, Length=259, Percent_Identity=28.957528957529, Blast_Score=102, Evalue=4e-23,
Organism=Escherichia coli, GI87082183, Length=250, Percent_Identity=27.6, Blast_Score=91, Evalue=7e-20,
Organism=Escherichia coli, GI1790281, Length=178, Percent_Identity=32.0224719101124, Blast_Score=87, Evalue=9e-19,
Organism=Escherichia coli, GI1788682, Length=219, Percent_Identity=31.9634703196347, Blast_Score=80, Evalue=2e-16,
Organism=Escherichia coli, GI1787660, Length=255, Percent_Identity=28.2352941176471, Blast_Score=69, Evalue=3e-13,
Organism=Caenorhabditis elegans, GI25145438, Length=248, Percent_Identity=32.258064516129, Blast_Score=112, Evalue=3e-25,
Organism=Caenorhabditis elegans, GI17554946, Length=250, Percent_Identity=31.6, Blast_Score=110, Evalue=1e-24,
Organism=Caenorhabditis elegans, GI17540714, Length=242, Percent_Identity=26.8595041322314, Blast_Score=100, Evalue=8e-22,
Organism=Caenorhabditis elegans, GI17536985, Length=260, Percent_Identity=23.8461538461538, Blast_Score=85, Evalue=5e-17,
Organism=Caenorhabditis elegans, GI17549921, Length=205, Percent_Identity=28.780487804878, Blast_Score=78, Evalue=5e-15,
Organism=Caenorhabditis elegans, GI17558304, Length=221, Percent_Identity=26.2443438914027, Blast_Score=74, Evalue=1e-13,
Organism=Caenorhabditis elegans, GI17540306, Length=246, Percent_Identity=28.8617886178862, Blast_Score=72, Evalue=2e-13,
Organism=Caenorhabditis elegans, GI17560910, Length=177, Percent_Identity=29.9435028248588, Blast_Score=70, Evalue=1e-12,
Organism=Caenorhabditis elegans, GI25144157, Length=219, Percent_Identity=26.027397260274, Blast_Score=67, Evalue=9e-12,
Organism=Caenorhabditis elegans, GI25144276, Length=200, Percent_Identity=27.5, Blast_Score=66, Evalue=2e-11,
Organism=Caenorhabditis elegans, GI17508951, Length=200, Percent_Identity=27.5, Blast_Score=66, Evalue=2e-11,
Organism=Caenorhabditis elegans, GI17508953, Length=200, Percent_Identity=27.5, Blast_Score=66, Evalue=3e-11,
Organism=Caenorhabditis elegans, GI17535521, Length=266, Percent_Identity=24.4360902255639, Blast_Score=65, Evalue=3e-11,
Organism=Caenorhabditis elegans, GI25144160, Length=201, Percent_Identity=25.8706467661692, Blast_Score=65, Evalue=4e-11,
Organism=Caenorhabditis elegans, GI17534483, Length=218, Percent_Identity=26.1467889908257, Blast_Score=65, Evalue=5e-11,
Organism=Drosophila melanogaster, GI20129971, Length=250, Percent_Identity=33.2, Blast_Score=124, Evalue=7e-29,
Organism=Drosophila melanogaster, GI24653477, Length=250, Percent_Identity=33.2, Blast_Score=124, Evalue=7e-29,
Organism=Drosophila melanogaster, GI24653139, Length=270, Percent_Identity=31.8518518518519, Blast_Score=113, Evalue=1e-25,
Organism=Drosophila melanogaster, GI19922422, Length=264, Percent_Identity=27.6515151515151, Blast_Score=99, Evalue=3e-21,
Organism=Drosophila melanogaster, GI19920382, Length=212, Percent_Identity=26.8867924528302, Blast_Score=70, Evalue=1e-12,
Organism=Drosophila melanogaster, GI24654903, Length=174, Percent_Identity=22.9885057471264, Blast_Score=67, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014748
- InterPro:   IPR001753
- InterPro:   IPR018376 [H]

Pfam domain/function: PF00378 ECH [H]

EC number: =4.2.1.55 [H]

Molecular weight: Translated: 31299; Mature: 31299

Theoretical pI: Translated: 6.75; Mature: 6.75

Prosite motif: PS00166 ENOYL_COA_HYDRATASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.4 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
5.4 %Cys+Met (Translated Protein)
2.4 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
5.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRRRAMTKMDEASNPCGGDIEAEMCQLMREQPPAEGVVDRVALQRHRNVALITLSHPQAQ
CCCHHHHHHHHCCCCCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHCCCEEEEEECCCCHH
NALNLASWRRLKRLLDDLAGESGLRAVVLRGAGDKAFAAGADIKEFPNTRMSAADAAEYN
HHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCHHCCCCCHHHCCCCCCCHHHHHHHH
ESLAVCLRALTTMPIPVIAAVRGLAVGGGCELATACDVCIATDDARFGIPLGKLGVTTGF
HHHHHHHHHHHHCCHHHHHHHHHHHCCCCCCHHHHCCEEEECCCCCCCCCHHHHCCCCCC
TEADTVARLIGPAALKYLLFSGELIGIEEAARWGLVQKVVAPQDLAAATAKLVGQVCRQS
CHHHHHHHHHHHHHHHHHHHCCCEECHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHH
AVTMRAAKVVANMHGRALTGADTDALIRFGVEAYEGADLREGVAAFSQGRPPKFDD
HHHHHHHHHHHHHCCCEECCCCHHHHHHHCHHHHCCCCHHHHHHHHHCCCCCCCCC
>Mature Secondary Structure
MRRRAMTKMDEASNPCGGDIEAEMCQLMREQPPAEGVVDRVALQRHRNVALITLSHPQAQ
CCCHHHHHHHHCCCCCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHCCCEEEEEECCCCHH
NALNLASWRRLKRLLDDLAGESGLRAVVLRGAGDKAFAAGADIKEFPNTRMSAADAAEYN
HHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCHHCCCCCHHHCCCCCCCHHHHHHHH
ESLAVCLRALTTMPIPVIAAVRGLAVGGGCELATACDVCIATDDARFGIPLGKLGVTTGF
HHHHHHHHHHHHCCHHHHHHHHHHHCCCCCCHHHHCCEEEECCCCCCCCCHHHHCCCCCC
TEADTVARLIGPAALKYLLFSGELIGIEEAARWGLVQKVVAPQDLAAATAKLVGQVCRQS
CHHHHHHHHHHHHHHHHHHHCCCEECHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHH
AVTMRAAKVVANMHGRALTGADTDALIRFGVEAYEGADLREGVAAFSQGRPPKFDD
HHHHHHHHHHHHHCCCEECCCCHHHHHHHCHHHHCCCCHHHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8655474; 11466286 [H]