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The map label for this gene is amiD

Identifier: 121639301

GI number: 121639301

Start: 3776185

End: 3777612

Strand: Direct

Name: amiD

Synonym: BCG_3446

Alternate gene names: 121639301

Gene position: 3776185-3777612 (Clockwise)

Preceding gene: 121639300

Following gene: 121639302

Centisome position: 86.32

GC content: 66.39

Gene sequence:

>1428_bases
ATGACCGATGCTGACAGTGCGGTCCCTCCCCGACTCGACGAGGACGCGATCTCGAAACTCGAGCTGACCGAGGTCGCCGA
CCTGATCCGCACCCGGCAACTGACGTCGGCAGAAGTGACCGAGTCGACGCTGCGGCGTATCGAAAGGCTTGACCCCCAGC
TGAAGAGCTACGCCTTCGTCATGCCGGAAACTGCGCTAGCGGCGGCACGTGCCGCCGACGCCGACATCGCGCGCGGCCAC
TACGAGGGTGTCCTGCACGGCGTACCGATCGGCGTGAAGGATCTCTGCTACACGGTCGACGCCCCGACCGCGGCCGGCAC
CACCATCTTTCGTGACTTTCGCCCGGCATACGACGCGACGGTTGTCGCGAGGTTGCGCGCGGCCGGCGCGGTGATCATCG
GCAAGCTGGCCATGACGGAGGGGGCCTATCTCGGCTATCACCCCAGTCTGCCGACCCCGGTCAATCCCTGGGACCCGACA
GCGTGGGCGGGCGTGTCCTCGAGCGGCTGCGGCGTGGCCACCGCGGCGGGATTGTGCTTCGGCTCGATCGGGTCGGACAC
CGGGGGGTCGATTCGCTTTCCGACGAGCATGTGCGGCGTCACCGGGATCAAACCGACGTGGGGCCGGGTCAGCCGTCACG
GCGTCGTCGAACTTGCGGCAAGCTACGACCACGTCGGGCCGATCACCCGTAGCGCTCACGATGCGGCGGTATTGCTCAGT
GTCATAGCGGGATCCGATATCCACGATCCCTCGTGCTCGGCGGAGCCCGTTCCGGACTATGCCGCCGACCTCGCCTTGAC
ACGGATTCCGCGTGTCGGGGTGGACTGGTCGCAGACGACGTCGTTTGACGAGGACACCACGGCGATGCTGGCCGATGTCG
TCAAAACGCTCGACGACATCGGATGGCCCGTCATCGACGTCAAGCTGCCCGCGCTTGCGCCGATGGTGGCAGCGTTCGGA
AAAATGCGCGCGGTCGAAACGGCGATCGCGCATGCCGACACCTACCCGGCGCGCGCCGACGAGTACGGGCCGATCATGCG
CGCAATGATCGACGCCGGACACAGGCTGGCTGCGGTGGAATATCAGACGCTGACCGAGCGGCGTCTGGAATTCACGCGAT
CGCTGCGTCGCGTGTTCCACGACGTGGACATCCTGCTGATGCCCAGCGCCGGAATTGCCTCGCCCACACTGGAAACCATG
CGCGGGCTCGGACAAGACCCGGAGCTGACCGCCAGACTGGCGATGCCGACAGCACCGTTCAACGTCAGCGGTAATCCCGC
GATATGCCTACCGGCGGGAACGACGGCGCGCGGAACGCCGCTCGGCGTCCAGTTCATCGGCCGTGAATTCGACGAGCACT
TGCTCGTCCGAGCCGGCCACGCATTTCAGCAAGTCACCGGGTATCATCGCCGACGCCCGCCGGTGTGA

Upstream 100 bases:

>100_bases
CTGATCCGGTTCGGTGTCGAAGCCTACGAGGGGGCGGACCTACGCGAAGGGGTGGCGGCCTTCAGCCAGGGACGCCCACC
CAAATTTGATGATTAGCGCC

Downstream 100 bases:

>100_bases
AAAACCCTCGGCCGCAAAAGGCTTGCGAATGTCGCACCGAAGGTCGCGGCGAATCGCCTTACTGGTATGTTTACGAACAC
AATCTGTGGCCATCAAGGGA

Product: putative amidase amiD

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 475; Mature: 474

Protein sequence:

>475_residues
MTDADSAVPPRLDEDAISKLELTEVADLIRTRQLTSAEVTESTLRRIERLDPQLKSYAFVMPETALAAARAADADIARGH
YEGVLHGVPIGVKDLCYTVDAPTAAGTTIFRDFRPAYDATVVARLRAAGAVIIGKLAMTEGAYLGYHPSLPTPVNPWDPT
AWAGVSSSGCGVATAAGLCFGSIGSDTGGSIRFPTSMCGVTGIKPTWGRVSRHGVVELAASYDHVGPITRSAHDAAVLLS
VIAGSDIHDPSCSAEPVPDYAADLALTRIPRVGVDWSQTTSFDEDTTAMLADVVKTLDDIGWPVIDVKLPALAPMVAAFG
KMRAVETAIAHADTYPARADEYGPIMRAMIDAGHRLAAVEYQTLTERRLEFTRSLRRVFHDVDILLMPSAGIASPTLETM
RGLGQDPELTARLAMPTAPFNVSGNPAICLPAGTTARGTPLGVQFIGREFDEHLLVRAGHAFQQVTGYHRRRPPV

Sequences:

>Translated_475_residues
MTDADSAVPPRLDEDAISKLELTEVADLIRTRQLTSAEVTESTLRRIERLDPQLKSYAFVMPETALAAARAADADIARGH
YEGVLHGVPIGVKDLCYTVDAPTAAGTTIFRDFRPAYDATVVARLRAAGAVIIGKLAMTEGAYLGYHPSLPTPVNPWDPT
AWAGVSSSGCGVATAAGLCFGSIGSDTGGSIRFPTSMCGVTGIKPTWGRVSRHGVVELAASYDHVGPITRSAHDAAVLLS
VIAGSDIHDPSCSAEPVPDYAADLALTRIPRVGVDWSQTTSFDEDTTAMLADVVKTLDDIGWPVIDVKLPALAPMVAAFG
KMRAVETAIAHADTYPARADEYGPIMRAMIDAGHRLAAVEYQTLTERRLEFTRSLRRVFHDVDILLMPSAGIASPTLETM
RGLGQDPELTARLAMPTAPFNVSGNPAICLPAGTTARGTPLGVQFIGREFDEHLLVRAGHAFQQVTGYHRRRPPV
>Mature_474_residues
TDADSAVPPRLDEDAISKLELTEVADLIRTRQLTSAEVTESTLRRIERLDPQLKSYAFVMPETALAAARAADADIARGHY
EGVLHGVPIGVKDLCYTVDAPTAAGTTIFRDFRPAYDATVVARLRAAGAVIIGKLAMTEGAYLGYHPSLPTPVNPWDPTA
WAGVSSSGCGVATAAGLCFGSIGSDTGGSIRFPTSMCGVTGIKPTWGRVSRHGVVELAASYDHVGPITRSAHDAAVLLSV
IAGSDIHDPSCSAEPVPDYAADLALTRIPRVGVDWSQTTSFDEDTTAMLADVVKTLDDIGWPVIDVKLPALAPMVAAFGK
MRAVETAIAHADTYPARADEYGPIMRAMIDAGHRLAAVEYQTLTERRLEFTRSLRRVFHDVDILLMPSAGIASPTLETMR
GLGQDPELTARLAMPTAPFNVSGNPAICLPAGTTARGTPLGVQFIGREFDEHLLVRAGHAFQQVTGYHRRRPPV

Specific function: Unknown

COG id: COG0154

COG function: function code J; Asp-tRNAAsn/Glu-tRNAGln amidotransferase A subunit and related amidases

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the amidase family

Homologues:

Organism=Homo sapiens, GI222831590, Length=496, Percent_Identity=28.6290322580645, Blast_Score=155, Evalue=1e-37,
Organism=Homo sapiens, GI195972892, Length=503, Percent_Identity=26.6401590457256, Blast_Score=119, Evalue=4e-27,
Organism=Homo sapiens, GI166795287, Length=236, Percent_Identity=27.1186440677966, Blast_Score=69, Evalue=1e-11,
Organism=Caenorhabditis elegans, GI17543272, Length=442, Percent_Identity=27.6018099547511, Blast_Score=122, Evalue=4e-28,
Organism=Caenorhabditis elegans, GI17537465, Length=493, Percent_Identity=23.3265720081136, Blast_Score=92, Evalue=7e-19,
Organism=Caenorhabditis elegans, GI17556264, Length=296, Percent_Identity=24.6621621621622, Blast_Score=75, Evalue=6e-14,
Organism=Saccharomyces cerevisiae, GI6319685, Length=401, Percent_Identity=26.1845386533666, Blast_Score=105, Evalue=2e-23,
Organism=Saccharomyces cerevisiae, GI6323950, Length=255, Percent_Identity=27.4509803921569, Blast_Score=88, Evalue=2e-18,
Organism=Drosophila melanogaster, GI24648113, Length=469, Percent_Identity=31.5565031982942, Blast_Score=151, Evalue=9e-37,
Organism=Drosophila melanogaster, GI161078093, Length=498, Percent_Identity=25.7028112449799, Blast_Score=117, Evalue=2e-26,
Organism=Drosophila melanogaster, GI24644968, Length=514, Percent_Identity=25.2918287937743, Blast_Score=111, Evalue=1e-24,
Organism=Drosophila melanogaster, GI24652985, Length=502, Percent_Identity=26.8924302788845, Blast_Score=92, Evalue=7e-19,
Organism=Drosophila melanogaster, GI19922090, Length=502, Percent_Identity=26.8924302788845, Blast_Score=92, Evalue=7e-19,
Organism=Drosophila melanogaster, GI24652981, Length=502, Percent_Identity=26.8924302788845, Blast_Score=92, Evalue=7e-19,
Organism=Drosophila melanogaster, GI24652983, Length=502, Percent_Identity=26.8924302788845, Blast_Score=92, Evalue=7e-19,
Organism=Drosophila melanogaster, GI21356731, Length=490, Percent_Identity=23.265306122449, Blast_Score=87, Evalue=2e-17,
Organism=Drosophila melanogaster, GI24648435, Length=235, Percent_Identity=30.2127659574468, Blast_Score=75, Evalue=7e-14,
Organism=Drosophila melanogaster, GI24648437, Length=235, Percent_Identity=30.2127659574468, Blast_Score=75, Evalue=7e-14,
Organism=Drosophila melanogaster, GI45550774, Length=235, Percent_Identity=30.2127659574468, Blast_Score=75, Evalue=7e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): AMI4_MYCBO (P63497)

Other databases:

- EMBL:   BX248345
- RefSeq:   NP_857050.1
- ProteinModelPortal:   P63497
- EnsemblBacteria:   EBMYCT00000015682
- GeneID:   1090948
- GenomeReviews:   BX248333_GR
- KEGG:   mbo:Mb3409
- GeneTree:   EBGT00050000014631
- HOGENOM:   HBG481888
- OMA:   YGAFELA
- ProtClustDB:   CLSK872172
- BioCyc:   MBOV233413:MB3409-MONOMER
- BRENDA:   3.5.1.4
- InterPro:   IPR000120
- InterPro:   IPR020556
- Gene3D:   G3DSA:3.90.1300.10
- PANTHER:   PTHR11895

Pfam domain/function: PF01425 Amidase; SSF75304 Amidase_sig_enz

EC number: =3.5.1.4

Molecular weight: Translated: 50646; Mature: 50515

Theoretical pI: Translated: 5.74; Mature: 5.74

Prosite motif: PS00571 AMIDASES

Important sites: ACT_SITE 93-93 ACT_SITE 166-166 ACT_SITE 190-190

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTDADSAVPPRLDEDAISKLELTEVADLIRTRQLTSAEVTESTLRRIERLDPQLKSYAFV
CCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCHHHHHEEEE
MPETALAAARAADADIARGHYEGVLHGVPIGVKDLCYTVDAPTAAGTTIFRDFRPAYDAT
CCHHHHHHHHHCCHHHHHHHHHHHHCCCCCCHHHHEEECCCCCCCCHHHHHHCCCHHHHH
VVARLRAAGAVIIGKLAMTEGAYLGYHPSLPTPVNPWDPTAWAGVSSSGCGVATAAGLCF
HHHHHHHCCHHEEHHHHHCCCCEECCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHH
GSIGSDTGGSIRFPTSMCGVTGIKPTWGRVSRHGVVELAASYDHVGPITRSAHDAAVLLS
HCCCCCCCCCEECCHHHCCCCCCCCCHHHHHHCCHHHHHHCCCCCCCCCCCHHHHHHHHH
VIAGSDIHDPSCSAEPVPDYAADLALTRIPRVGVDWSQTTSFDEDTTAMLADVVKTLDDI
HHHCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHC
GWPVIDVKLPALAPMVAAFGKMRAVETAIAHADTYPARADEYGPIMRAMIDAGHRLAAVE
CCCEEEEECHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHCCCCEEEEH
YQTLTERRLEFTRSLRRVFHDVDILLMPSAGIASPTLETMRGLGQDPELTARLAMPTAPF
HHHHHHHHHHHHHHHHHHHHCCCEEEECCCCCCCCHHHHHHCCCCCCCCEEEEECCCCCC
NVSGNPAICLPAGTTARGTPLGVQFIGREFDEHLLVRAGHAFQQVTGYHRRRPPV
CCCCCCEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCC
>Mature Secondary Structure 
TDADSAVPPRLDEDAISKLELTEVADLIRTRQLTSAEVTESTLRRIERLDPQLKSYAFV
CCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCHHHHHEEEE
MPETALAAARAADADIARGHYEGVLHGVPIGVKDLCYTVDAPTAAGTTIFRDFRPAYDAT
CCHHHHHHHHHCCHHHHHHHHHHHHCCCCCCHHHHEEECCCCCCCCHHHHHHCCCHHHHH
VVARLRAAGAVIIGKLAMTEGAYLGYHPSLPTPVNPWDPTAWAGVSSSGCGVATAAGLCF
HHHHHHHCCHHEEHHHHHCCCCEECCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHH
GSIGSDTGGSIRFPTSMCGVTGIKPTWGRVSRHGVVELAASYDHVGPITRSAHDAAVLLS
HCCCCCCCCCEECCHHHCCCCCCCCCHHHHHHCCHHHHHHCCCCCCCCCCCHHHHHHHHH
VIAGSDIHDPSCSAEPVPDYAADLALTRIPRVGVDWSQTTSFDEDTTAMLADVVKTLDDI
HHHCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHC
GWPVIDVKLPALAPMVAAFGKMRAVETAIAHADTYPARADEYGPIMRAMIDAGHRLAAVE
CCCEEEEECHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHCCCCEEEEH
YQTLTERRLEFTRSLRRVFHDVDILLMPSAGIASPTLETMRGLGQDPELTARLAMPTAPF
HHHHHHHHHHHHHHHHHHHHCCCEEEECCCCCCCCHHHHHHCCCCCCCCEEEEECCCCCC
NVSGNPAICLPAGTTARGTPLGVQFIGREFDEHLLVRAGHAFQQVTGYHRRRPPV
CCCCCCEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12788972