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The map label for this gene is dlaT [H]

Identifier: 121638096

GI number: 121638096

Start: 2460609

End: 2462270

Strand: Direct

Name: dlaT [H]

Synonym: BCG_2231

Alternate gene names: 121638096

Gene position: 2460609-2462270 (Clockwise)

Preceding gene: 121638094

Following gene: 121638097

Centisome position: 56.25

GC content: 67.69

Gene sequence:

>1662_bases
ATGGCCTTCTCCGTCCAGATGCCGGCACTCGGTGAGAGCGTCACCGAGGGGACGGTTACCCGCTGGCTCAAACAGGAAGG
CGACACGGTCGAACTCGACGAGCCCCTCGTGGAGGTGTCGACCGACAAGGTCGACACCGAAATCCCCTCGCCGGCCGCGG
GTGTGCTGACCAAGATCATCGCCCAGGAGGATGACACGGTCGAGGTCGGCGGCGAGCTCGCTGTCATTGGCGACGCCAAG
GATGCCGGCGAGGCCGCGGCCCCGGCACCCGAGAAAGTCCCTGCGGCCCAACCCGAGTCCAAGCCGGCACCCGAACCACC
ACCGGTCCAACCGACGTCCGGAGCGCCTGCTGGTGGCGATGCCAAGCCGGTGCTGATGCCCGAGCTCGGCGAATCGGTGA
CCGAGGGGACCGTCATTCGTTGGCTGAAGAAGATCGGGGATTCGGTTCAGGTTGACGAGCCACTCGTGGAGGTGTCCACC
GACAAGGTGGACACCGAGATCCCGTCCCCGGTGGCTGGGGTCTTGGTCAGTATCAGCGCCGACGAGGACGCCACGGTGCC
CGTCGGCGGCGAGTTGGCCCGGATCGGTGTCGCTGCCGACATCGGCGCCGCGCCCGCCCCCAAGCCCGCACCCAAGCCCG
TCCCCGAGCCAGCGCCGACGCCGAAGGCCGAACCCGCACCATCGCCGCCGGCGGCCCAGCCAGCCGGTGCGGCCGAGGGC
GCACCGTACGTGACGCCGCTGGTGCGAAAGCTGGCGTCGGAAAACAACATCGACCTCGCCGGGGTGACCGGCACCGGAGT
GGGTGGTCGCATCCGCAAACAGGATGTGCTGGCCGCGGCTGAGCAAAAGAAGCGGGCGAAAGCACCGGCGCCGGCCGCCC
AGGCCGCCGCCGCGCCGGCCCCGAAAGCGCCGCCTGCCCCTGCGCCGGCGTTGGCACATCTACGGGGCACCACCCAGAAG
GCCAGCCGGATTCGTCAGATCACCGCCAACAAGACCCGCGAATCTTTGCAGGCAACGGCACAGCTGACACAAACCCATGA
GGTCGACATGACCAAGATCGTGGGGCTACGGGCCCGGGCCAAGGCGGCGTTCGCCGAGCGTGAGGGCGTGAACCTGACCT
TCCTGCCGTTCTTCGCCAAGGCCGTGATCGATGCCCTCAAGATTCACCCGAACATCAACGCTAGCTACAACGAGGACACC
AAGGAGATCACCTACTACGACGCCGAGCACCTAGGATTCGCTGTCGACACCGAGCAGGGCCTGCTCTCCCCGGTCATCCA
CGACGCCGGCGATCTGTCACTGGCCGGTCTGGCGCGGGCGATCGCCGATATCGCGGCCCGTGCCCGGTCGGGCAACCTGA
AACCCGACGAGTTGTCCGGCGGCACCTTCACCATCACCAACATCGGTAGCCAGGGCGCGTTGTTCGACACCCCGATCCTG
GTTCCGCCGCAGGCCGCCATGCTGGGCACCGGGGCGATCGTCAAGCGGCCGCGGGTGGTCGTCGATGCCAGCGGCAACGA
GTCGATCGGGGTGCGCTCGGTCTGCTACGTCCCGTTGACCTATGACCATCGGCTCATCGACGGCGCCGACGCCGGACGTT
TCCTCACCACGATCAAGCACCGCCTCGAAGAGGGAGCGTTCGAGGCCGATTTAGGACTGTGA

Upstream 100 bases:

>100_bases
GGTTTCGACATCCACCTGGGTTCCGCACCCGGTGCGCGACCGTGTGATAGGCCAGAGGTGGACCTGCGCCGACCGACGAT
CGATCGAGGAGTCAACAGAA

Downstream 100 bases:

>100_bases
TGGCCAACGCCGTTGTCGCGATCGCGGGTTCGTCTGGCTTGATCGGCTCTGCCCTGACCGCGGCGCTGCGCGCGGCCGAC
CACACGGTGCTGCGGATCGT

Product: dihydrolipoamide acetyltransferase

Products: NA

Alternate protein names: 2-oxoglutarate dehydrogenase complex component E2; OGDC-E2; Dihydrolipoamide succinyltransferase component of 2-oxoglutarate dehydrogenase complex [H]

Number of amino acids: Translated: 553; Mature: 552

Protein sequence:

>553_residues
MAFSVQMPALGESVTEGTVTRWLKQEGDTVELDEPLVEVSTDKVDTEIPSPAAGVLTKIIAQEDDTVEVGGELAVIGDAK
DAGEAAAPAPEKVPAAQPESKPAPEPPPVQPTSGAPAGGDAKPVLMPELGESVTEGTVIRWLKKIGDSVQVDEPLVEVST
DKVDTEIPSPVAGVLVSISADEDATVPVGGELARIGVAADIGAAPAPKPAPKPVPEPAPTPKAEPAPSPPAAQPAGAAEG
APYVTPLVRKLASENNIDLAGVTGTGVGGRIRKQDVLAAAEQKKRAKAPAPAAQAAAAPAPKAPPAPAPALAHLRGTTQK
ASRIRQITANKTRESLQATAQLTQTHEVDMTKIVGLRARAKAAFAEREGVNLTFLPFFAKAVIDALKIHPNINASYNEDT
KEITYYDAEHLGFAVDTEQGLLSPVIHDAGDLSLAGLARAIADIAARARSGNLKPDELSGGTFTITNIGSQGALFDTPIL
VPPQAAMLGTGAIVKRPRVVVDASGNESIGVRSVCYVPLTYDHRLIDGADAGRFLTTIKHRLEEGAFEADLGL

Sequences:

>Translated_553_residues
MAFSVQMPALGESVTEGTVTRWLKQEGDTVELDEPLVEVSTDKVDTEIPSPAAGVLTKIIAQEDDTVEVGGELAVIGDAK
DAGEAAAPAPEKVPAAQPESKPAPEPPPVQPTSGAPAGGDAKPVLMPELGESVTEGTVIRWLKKIGDSVQVDEPLVEVST
DKVDTEIPSPVAGVLVSISADEDATVPVGGELARIGVAADIGAAPAPKPAPKPVPEPAPTPKAEPAPSPPAAQPAGAAEG
APYVTPLVRKLASENNIDLAGVTGTGVGGRIRKQDVLAAAEQKKRAKAPAPAAQAAAAPAPKAPPAPAPALAHLRGTTQK
ASRIRQITANKTRESLQATAQLTQTHEVDMTKIVGLRARAKAAFAEREGVNLTFLPFFAKAVIDALKIHPNINASYNEDT
KEITYYDAEHLGFAVDTEQGLLSPVIHDAGDLSLAGLARAIADIAARARSGNLKPDELSGGTFTITNIGSQGALFDTPIL
VPPQAAMLGTGAIVKRPRVVVDASGNESIGVRSVCYVPLTYDHRLIDGADAGRFLTTIKHRLEEGAFEADLGL
>Mature_552_residues
AFSVQMPALGESVTEGTVTRWLKQEGDTVELDEPLVEVSTDKVDTEIPSPAAGVLTKIIAQEDDTVEVGGELAVIGDAKD
AGEAAAPAPEKVPAAQPESKPAPEPPPVQPTSGAPAGGDAKPVLMPELGESVTEGTVIRWLKKIGDSVQVDEPLVEVSTD
KVDTEIPSPVAGVLVSISADEDATVPVGGELARIGVAADIGAAPAPKPAPKPVPEPAPTPKAEPAPSPPAAQPAGAAEGA
PYVTPLVRKLASENNIDLAGVTGTGVGGRIRKQDVLAAAEQKKRAKAPAPAAQAAAAPAPKAPPAPAPALAHLRGTTQKA
SRIRQITANKTRESLQATAQLTQTHEVDMTKIVGLRARAKAAFAEREGVNLTFLPFFAKAVIDALKIHPNINASYNEDTK
EITYYDAEHLGFAVDTEQGLLSPVIHDAGDLSLAGLARAIADIAARARSGNLKPDELSGGTFTITNIGSQGALFDTPILV
PPQAAMLGTGAIVKRPRVVVDASGNESIGVRSVCYVPLTYDHRLIDGADAGRFLTTIKHRLEEGAFEADLGL

Specific function: The 2-oxoglutarate dehydrogenase complex catalyzes the overall conversion of 2-oxoglutarate to succinyl-CoA and CO(2). It contains multiple copies of three enzymatic components:2- oxoglutarate dehydrogenase (E1), dihydrolipoamide succinyltransferase (E2)

COG id: COG0508

COG function: function code C; Pyruvate/2-oxoglutarate dehydrogenase complex, dihydrolipoamide acyltransferase (E2) component, and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 lipoyl-binding domains [H]

Homologues:

Organism=Homo sapiens, GI110671329, Length=483, Percent_Identity=30.2277432712215, Blast_Score=176, Evalue=4e-44,
Organism=Homo sapiens, GI19923748, Length=226, Percent_Identity=41.1504424778761, Blast_Score=170, Evalue=3e-42,
Organism=Homo sapiens, GI31711992, Length=570, Percent_Identity=29.2982456140351, Blast_Score=166, Evalue=4e-41,
Organism=Homo sapiens, GI203098816, Length=467, Percent_Identity=28.4796573875803, Blast_Score=165, Evalue=1e-40,
Organism=Homo sapiens, GI203098753, Length=453, Percent_Identity=27.8145695364238, Blast_Score=155, Evalue=7e-38,
Organism=Homo sapiens, GI260898739, Length=156, Percent_Identity=33.3333333333333, Blast_Score=93, Evalue=8e-19,
Organism=Escherichia coli, GI1786946, Length=422, Percent_Identity=37.6777251184834, Blast_Score=243, Evalue=2e-65,
Organism=Escherichia coli, GI1786305, Length=545, Percent_Identity=31.1926605504587, Blast_Score=184, Evalue=1e-47,
Organism=Caenorhabditis elegans, GI25146366, Length=429, Percent_Identity=31.4685314685315, Blast_Score=178, Evalue=8e-45,
Organism=Caenorhabditis elegans, GI17537937, Length=431, Percent_Identity=27.3781902552204, Blast_Score=163, Evalue=2e-40,
Organism=Caenorhabditis elegans, GI17560088, Length=446, Percent_Identity=32.0627802690583, Blast_Score=143, Evalue=2e-34,
Organism=Caenorhabditis elegans, GI17538894, Length=326, Percent_Identity=26.9938650306748, Blast_Score=79, Evalue=7e-15,
Organism=Saccharomyces cerevisiae, GI6320352, Length=427, Percent_Identity=32.0843091334895, Blast_Score=193, Evalue=6e-50,
Organism=Saccharomyces cerevisiae, GI6324258, Length=442, Percent_Identity=26.0180995475113, Blast_Score=126, Evalue=1e-29,
Organism=Drosophila melanogaster, GI18859875, Length=437, Percent_Identity=28.3752860411899, Blast_Score=147, Evalue=1e-35,
Organism=Drosophila melanogaster, GI24645909, Length=247, Percent_Identity=36.0323886639676, Blast_Score=142, Evalue=6e-34,
Organism=Drosophila melanogaster, GI24582497, Length=435, Percent_Identity=27.5862068965517, Blast_Score=106, Evalue=3e-23,
Organism=Drosophila melanogaster, GI20129315, Length=447, Percent_Identity=27.0693512304251, Blast_Score=105, Evalue=6e-23,

Paralogues:

None

Copy number: 420 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 3096 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 3,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003016
- InterPro:   IPR001078
- InterPro:   IPR014276
- InterPro:   IPR000089
- InterPro:   IPR023213
- InterPro:   IPR004167
- InterPro:   IPR011053 [H]

Pfam domain/function: PF00198 2-oxoacid_dh; PF00364 Biotin_lipoyl; PF02817 E3_binding [H]

EC number: =2.3.1.61 [H]

Molecular weight: Translated: 57074; Mature: 56943

Theoretical pI: Translated: 4.64; Mature: 4.64

Prosite motif: PS50968 BIOTINYL_LIPOYL ; PS00189 LIPOYL

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
0.9 %Met     (Translated Protein)
1.1 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
0.7 %Met     (Mature Protein)
0.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAFSVQMPALGESVTEGTVTRWLKQEGDTVELDEPLVEVSTDKVDTEIPSPAAGVLTKII
CCEEECCCCCCCHHHHHHHHHHHHHCCCEEECCCCCEEECCCCCCCCCCCCHHHHHHHHH
AQEDDTVEVGGELAVIGDAKDAGEAAAPAPEKVPAAQPESKPAPEPPPVQPTSGAPAGGD
HCCCCCEEECCEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
AKPVLMPELGESVTEGTVIRWLKKIGDSVQVDEPLVEVSTDKVDTEIPSPVAGVLVSISA
CCCEECHHHCCHHHHHHHHHHHHHHCCCCCCCCCHHEECCCCCCCCCCCCCEEEEEEEEC
DEDATVPVGGELARIGVAADIGAAPAPKPAPKPVPEPAPTPKAEPAPSPPAAQPAGAAEG
CCCCCCCCCCCEEEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
APYVTPLVRKLASENNIDLAGVTGTGVGGRIRKQDVLAAAEQKKRAKAPAPAAQAAAAPA
CCHHHHHHHHHHCCCCCEEEEECCCCCCCCCHHHHHHHHHHHHHHCCCCCCHHHHHCCCC
PKAPPAPAPALAHLRGTTQKASRIRQITANKTRESLQATAQLTQTHEVDMTKIVGLRARA
CCCCCCCCCHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHH
KAAFAEREGVNLTFLPFFAKAVIDALKIHPNINASYNEDTKEITYYDAEHLGFAVDTEQG
HHHHHHHCCCCEEEHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEEEEHHHCCEEEECCCC
LLSPVIHDAGDLSLAGLARAIADIAARARSGNLKPDELSGGTFTITNIGSQGALFDTPIL
HHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEEECCCCCCEECCCEE
VPPQAAMLGTGAIVKRPRVVVDASGNESIGVRSVCYVPLTYDHRLIDGADAGRFLTTIKH
CCCCHHHHCCCHHHCCCEEEEECCCCCCCCCEEEEEEEECCCCEEECCCCCHHHHHHHHH
RLEEGAFEADLGL
HHHHCCCHHCCCC
>Mature Secondary Structure 
AFSVQMPALGESVTEGTVTRWLKQEGDTVELDEPLVEVSTDKVDTEIPSPAAGVLTKII
CEEECCCCCCCHHHHHHHHHHHHHCCCEEECCCCCEEECCCCCCCCCCCCHHHHHHHHH
AQEDDTVEVGGELAVIGDAKDAGEAAAPAPEKVPAAQPESKPAPEPPPVQPTSGAPAGGD
HCCCCCEEECCEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
AKPVLMPELGESVTEGTVIRWLKKIGDSVQVDEPLVEVSTDKVDTEIPSPVAGVLVSISA
CCCEECHHHCCHHHHHHHHHHHHHHCCCCCCCCCHHEECCCCCCCCCCCCCEEEEEEEEC
DEDATVPVGGELARIGVAADIGAAPAPKPAPKPVPEPAPTPKAEPAPSPPAAQPAGAAEG
CCCCCCCCCCCEEEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
APYVTPLVRKLASENNIDLAGVTGTGVGGRIRKQDVLAAAEQKKRAKAPAPAAQAAAAPA
CCHHHHHHHHHHCCCCCEEEEECCCCCCCCCHHHHHHHHHHHHHHCCCCCCHHHHHCCCC
PKAPPAPAPALAHLRGTTQKASRIRQITANKTRESLQATAQLTQTHEVDMTKIVGLRARA
CCCCCCCCCHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHH
KAAFAEREGVNLTFLPFFAKAVIDALKIHPNINASYNEDTKEITYYDAEHLGFAVDTEQG
HHHHHHHCCCCEEEHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEEEEHHHCCEEEECCCC
LLSPVIHDAGDLSLAGLARAIADIAARARSGNLKPDELSGGTFTITNIGSQGALFDTPIL
HHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEEECCCCCCEECCCEE
VPPQAAMLGTGAIVKRPRVVVDASGNESIGVRSVCYVPLTYDHRLIDGADAGRFLTTIKH
CCCCHHHHCCCHHHCCCEEEEECCCCCCCCCEEEEEEEECCCCEEECCCCCHHHHHHHHH
RLEEGAFEADLGL
HHHHCCCHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12788972 [H]