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The map label for this gene is cysS2

Identifier: 121638012

GI number: 121638012

Start: 2371225

End: 2372469

Strand: Reverse

Name: cysS2

Synonym: BCG_2147c

Alternate gene names: 121638012

Gene position: 2372469-2371225 (Counterclockwise)

Preceding gene: 121638013

Following gene: 121638011

Centisome position: 54.23

GC content: 65.62

Gene sequence:

>1245_bases
ATGCAGTCGTGGTATTGCCCACCGGTTCCGGTGTTGCCGGGACGAGGCCCGCAGCTACGGCTGTACGACAGCGCCGACCG
GCAGGTCCGTCCGGTGGCGCCCGGATCTAAGGCCACCATGTACGTCTGCGGGATCACGCCCTACGACGCCACGCATCTGG
GCCATGCTGCCACCTATGTGACGTTCGACCTGATCCATCGGCTGTGGCTGGATCTCGGTCATGAATTGCACTATGTCCAG
AACATCACCGACATCGACGATCCACTATTTGAGCGCGCGGATCGCGACGGTGTCGACTGGCGTGACCTTGCCCAAGCCGA
GGTCGCCCTGTTCTGTGAGGACATGGCGGCGCTGCGGGTGCTACCACCGCAAGACTACGTGGGGGCCACCGAAGCGATTG
CTGAAATGGTCGAGCTCATCGAAAAAATGCTGGCGTGCGGGGCGGCCTATGTCATAGACCGGGAAATGGGAGAGTACCAG
GACATCTACTTCCGCGCTGACGCCACCCTGCAGTTCGGCTACGAGTCAGGGTATGACCGTGACACCATGCTGCGGCTGTG
CGAGGAACGTGGCGGCGATCCGCGGCGCCCCGGCAAGAGCGACGAACTCGACGCGTTGTTGTGGCGGGCCGCGCGGCCCG
GTGAGCCCAGCTGGCCGTCCCCGTTCGGGCCTGGCCGGCCAGGCTGGCATGTCGAGTGCGCAGCCATCGCGCTCAGTCGT
ATCGGAAGCGGCCTCGACATCCAGGGCGGTGGTAGCGATCTGATCTTTCCGCACCACGAGTTCACCGCTGCGCACGCCGA
ATGTGTCAGCGGCGAACGGCGATTCGCGCGGCACTACGTGCATGCCGGGATGATCGGCTGGGACGGGCACAAGATGTCAA
AGAGCCGCGGCAACCTCGTGCTGGTGTCGGCGCTGCGTGCGCAGGACGTTGAGCCATCGGCGGTTCGGCTGGGTTTGCTC
GCCGGACACTACCGAGCCGATCGGTTCTGGAGCCAGCAGGTGCTTGACGAGGCGACCGCCCGGCTGCACCGTTGGCGCAC
CGCAACCGCACTTCCCGCCGGTCCGGCCGCAGTTGACGTTGTCGCTCGGGTGCGCCGCTACCTGGCCGACGATCTCGATA
CGCCCAAAGCGATTGCCGCACTGGATGGTTGGGTCACCGATGCGGTGGAGTACGGCGGCCACGATGCCGGGGCGCCGAAG
TTGGTGGCGACGGCGATCGATGCCCTGCTCGGGGTGGACCTGTAG

Upstream 100 bases:

>100_bases
GGCGCCGATCCTGCTCGGTGCCATCGCCGACGCGTGGCGCTGATCGGCGCGCGGGCTGCTCAGAAAGGCTGGAGTCACCG
GGCCGTTTAGAGTCGAGGAC

Downstream 100 bases:

>100_bases
CCGGCGCGGCGGTACGTTTTCGGCCATGACATCGCTGCAAGGCAAGGTCGTCTTCATTACCGGTGCTGCCCGGGGAATCG
GGGCTGAGGTCGCCCGTCGG

Product: cysteinyl-tRNA synthetase

Products: NA

Alternate protein names: L-Cys:GlcN-Ins ligase; Mycothiol ligase; MSH ligase

Number of amino acids: Translated: 414; Mature: 414

Protein sequence:

>414_residues
MQSWYCPPVPVLPGRGPQLRLYDSADRQVRPVAPGSKATMYVCGITPYDATHLGHAATYVTFDLIHRLWLDLGHELHYVQ
NITDIDDPLFERADRDGVDWRDLAQAEVALFCEDMAALRVLPPQDYVGATEAIAEMVELIEKMLACGAAYVIDREMGEYQ
DIYFRADATLQFGYESGYDRDTMLRLCEERGGDPRRPGKSDELDALLWRAARPGEPSWPSPFGPGRPGWHVECAAIALSR
IGSGLDIQGGGSDLIFPHHEFTAAHAECVSGERRFARHYVHAGMIGWDGHKMSKSRGNLVLVSALRAQDVEPSAVRLGLL
AGHYRADRFWSQQVLDEATARLHRWRTATALPAGPAAVDVVARVRRYLADDLDTPKAIAALDGWVTDAVEYGGHDAGAPK
LVATAIDALLGVDL

Sequences:

>Translated_414_residues
MQSWYCPPVPVLPGRGPQLRLYDSADRQVRPVAPGSKATMYVCGITPYDATHLGHAATYVTFDLIHRLWLDLGHELHYVQ
NITDIDDPLFERADRDGVDWRDLAQAEVALFCEDMAALRVLPPQDYVGATEAIAEMVELIEKMLACGAAYVIDREMGEYQ
DIYFRADATLQFGYESGYDRDTMLRLCEERGGDPRRPGKSDELDALLWRAARPGEPSWPSPFGPGRPGWHVECAAIALSR
IGSGLDIQGGGSDLIFPHHEFTAAHAECVSGERRFARHYVHAGMIGWDGHKMSKSRGNLVLVSALRAQDVEPSAVRLGLL
AGHYRADRFWSQQVLDEATARLHRWRTATALPAGPAAVDVVARVRRYLADDLDTPKAIAALDGWVTDAVEYGGHDAGAPK
LVATAIDALLGVDL
>Mature_414_residues
MQSWYCPPVPVLPGRGPQLRLYDSADRQVRPVAPGSKATMYVCGITPYDATHLGHAATYVTFDLIHRLWLDLGHELHYVQ
NITDIDDPLFERADRDGVDWRDLAQAEVALFCEDMAALRVLPPQDYVGATEAIAEMVELIEKMLACGAAYVIDREMGEYQ
DIYFRADATLQFGYESGYDRDTMLRLCEERGGDPRRPGKSDELDALLWRAARPGEPSWPSPFGPGRPGWHVECAAIALSR
IGSGLDIQGGGSDLIFPHHEFTAAHAECVSGERRFARHYVHAGMIGWDGHKMSKSRGNLVLVSALRAQDVEPSAVRLGLL
AGHYRADRFWSQQVLDEATARLHRWRTATALPAGPAAVDVVARVRRYLADDLDTPKAIAALDGWVTDAVEYGGHDAGAPK
LVATAIDALLGVDL

Specific function: Catalyzes the ATP-dependent condensation of GlcN-Ins and L-cysteine to form L-Cys-GlcN-Ins

COG id: COG0215

COG function: function code J; Cysteinyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I aminoacyl-tRNA synthetase family. MshC subfamily

Homologues:

Organism=Homo sapiens, GI13375695, Length=392, Percent_Identity=34.4387755102041, Blast_Score=191, Evalue=1e-48,
Organism=Homo sapiens, GI303304987, Length=238, Percent_Identity=32.7731092436975, Blast_Score=132, Evalue=4e-31,
Organism=Homo sapiens, GI21269877, Length=238, Percent_Identity=32.7731092436975, Blast_Score=132, Evalue=5e-31,
Organism=Homo sapiens, GI62240992, Length=238, Percent_Identity=32.7731092436975, Blast_Score=132, Evalue=6e-31,
Organism=Homo sapiens, GI10835051, Length=238, Percent_Identity=32.7731092436975, Blast_Score=132, Evalue=6e-31,
Organism=Escherichia coli, GI1786737, Length=403, Percent_Identity=36.9727047146402, Blast_Score=251, Evalue=7e-68,
Organism=Caenorhabditis elegans, GI17509797, Length=262, Percent_Identity=32.824427480916, Blast_Score=129, Evalue=3e-30,
Organism=Caenorhabditis elegans, GI17509795, Length=262, Percent_Identity=32.824427480916, Blast_Score=129, Evalue=3e-30,
Organism=Saccharomyces cerevisiae, GI6324082, Length=315, Percent_Identity=28.2539682539683, Blast_Score=138, Evalue=2e-33,
Organism=Drosophila melanogaster, GI19922218, Length=393, Percent_Identity=33.3333333333333, Blast_Score=181, Evalue=8e-46,
Organism=Drosophila melanogaster, GI19922364, Length=242, Percent_Identity=31.8181818181818, Blast_Score=126, Evalue=3e-29,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MSHC_MYCBO (P67018)

Other databases:

- EMBL:   BX248341
- RefSeq:   NP_855803.1
- ProteinModelPortal:   P67018
- SMR:   P67018
- EnsemblBacteria:   EBMYCT00000016336
- GeneID:   1091211
- GenomeReviews:   BX248333_GR
- KEGG:   mbo:Mb2154c
- GeneTree:   EBGT00050000016491
- HOGENOM:   HBG327651
- OMA:   ALFREDM
- ProtClustDB:   PRK12418
- BioCyc:   MBOV233413:MB2154C-MONOMER
- BRENDA:   6.1.1.16
- GO:   GO:0005737
- HAMAP:   MF_01697
- InterPro:   IPR015803
- InterPro:   IPR017812
- InterPro:   IPR014729
- InterPro:   IPR009080
- Gene3D:   G3DSA:3.40.50.620
- PANTHER:   PTHR10890
- PRINTS:   PR00983
- TIGRFAMs:   TIGR03447

Pfam domain/function: PF01406 tRNA-synt_1e; SSF47323 tRNAsyn_1a_bind

EC number: =6.3.1.13

Molecular weight: Translated: 45595; Mature: 45595

Theoretical pI: Translated: 5.17; Mature: 5.17

Prosite motif: NA

Important sites: BINDING 58-58 BINDING 229-229 BINDING 285-285

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQSWYCPPVPVLPGRGPQLRLYDSADRQVRPVAPGSKATMYVCGITPYDATHLGHAATYV
CCCCCCCCCCCCCCCCCEEEEECCCCCEEECCCCCCCCEEEEEECCCCCHHHHCHHHHHH
TFDLIHRLWLDLGHELHYVQNITDIDDPLFERADRDGVDWRDLAQAEVALFCEDMAALRV
HHHHHHHHHHHHCCHHHHHHCCCCCCCHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHEE
LPPQDYVGATEAIAEMVELIEKMLACGAAYVIDREMGEYQDIYFRADATLQFGYESGYDR
CCCHHHCCHHHHHHHHHHHHHHHHHCCHHHHHHHHCCCCEEEEEEECCEEEECCCCCCCH
DTMLRLCEERGGDPRRPGKSDELDALLWRAARPGEPSWPSPFGPGRPGWHVECAAIALSR
HHHHHHHHHCCCCCCCCCCCCHHHHHHHHCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHH
IGSGLDIQGGGSDLIFPHHEFTAAHAECVSGERRFARHYVHAGMIGWDGHKMSKSRGNLV
CCCCCEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCEECCCCCCCCCCCCEE
LVSALRAQDVEPSAVRLGLLAGHYRADRFWSQQVLDEATARLHRWRTATALPAGPAAVDV
EEEEHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHH
VARVRRYLADDLDTPKAIAALDGWVTDAVEYGGHDAGAPKLVATAIDALLGVDL
HHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MQSWYCPPVPVLPGRGPQLRLYDSADRQVRPVAPGSKATMYVCGITPYDATHLGHAATYV
CCCCCCCCCCCCCCCCCEEEEECCCCCEEECCCCCCCCEEEEEECCCCCHHHHCHHHHHH
TFDLIHRLWLDLGHELHYVQNITDIDDPLFERADRDGVDWRDLAQAEVALFCEDMAALRV
HHHHHHHHHHHHCCHHHHHHCCCCCCCHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHEE
LPPQDYVGATEAIAEMVELIEKMLACGAAYVIDREMGEYQDIYFRADATLQFGYESGYDR
CCCHHHCCHHHHHHHHHHHHHHHHHCCHHHHHHHHCCCCEEEEEEECCEEEECCCCCCCH
DTMLRLCEERGGDPRRPGKSDELDALLWRAARPGEPSWPSPFGPGRPGWHVECAAIALSR
HHHHHHHHHCCCCCCCCCCCCHHHHHHHHCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHH
IGSGLDIQGGGSDLIFPHHEFTAAHAECVSGERRFARHYVHAGMIGWDGHKMSKSRGNLV
CCCCCEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCEECCCCCCCCCCCCEE
LVSALRAQDVEPSAVRLGLLAGHYRADRFWSQQVLDEATARLHRWRTATALPAGPAAVDV
EEEEHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHH
VARVRRYLADDLDTPKAIAALDGWVTDAVEYGGHDAGAPKLVATAIDALLGVDL
HHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12788972