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The map label for this gene is hisH

Identifier: 121637509

GI number: 121637509

Start: 1807911

End: 1808531

Strand: Direct

Name: hisH

Synonym: BCG_1640

Alternate gene names: 121637509

Gene position: 1807911-1808531 (Clockwise)

Preceding gene: 121637508

Following gene: 121637510

Centisome position: 41.33

GC content: 64.25

Gene sequence:

>621_bases
GTGACAGCAAAATCGGTTGTAGTCCTTGACTACGGCTCAGGAAACCTGCGGTCGGCCCAACGTGCGCTGCAACGAGTAGG
CGCCGAGGTCGAAGTAACCGCCGATACCGACGCCGCAATGACCGCTGACGGACTGGTGGTGCCGGGCGTCGGTGCTTTCG
CGGCGTGCATGGCGGGCCTGCGCAAGATCAGCGGAGAGCGAATCATCGCCGAGCGGGTGGCCGCCGGCCGCCCGGTGCTG
GGGGTCTGTGTCGGTATGCAGATTCTGTTTGCTTGCGGGGTCGAATTCGGTGTGCAGACGCCAGGCTGCGGGCACTGGCC
GGGGGCGGTCATTCGACTTGAGGCCCCGGTGATTCCGCACATGGGCTGGAATGTCGTGGATTCCGCTGCGGGCAGCGCGC
TGTTCAAAGGGTTGGACGTCGACGCCCGGTTTTATTTCGTGCATTCCTATGCCGCGCAGCGATGGGAAGGCTCACCCGAC
GCGCTGCTGACCTGGGCCACATATCGGGCGCCGTTCCTCGCTGCGGTGGAGGACGGCGCATTGGCCGCCACCCAGTTTCA
TCCGGAGAAGAGTGGCGATGCCGGTGCAGCCGTACTGAGCAACTGGGTTGATGGACTTTAA

Upstream 100 bases:

>100_bases
ATATCACCGAAGCTCAATACAAGGCCGTCGCGCGCGCGTTGCGTCAAGCGGTCGAGCCAGATCCTCGGGTGTCAGGCGTG
CCGTCCACCAAAGGTGCTCT

Downstream 100 bases:

>100_bases
AGGATACTGGTGATGCCGCTGATACTTTTGCCCGCCGTCGACGTGGTCGAGGGTCGTGCCGTGCGCCTCGTTCAAGGGAA
GGCCGGCAGCCAAACCGAGT

Product: imidazole glycerol phosphate synthase subunit HisH

Products: NA

Alternate protein names: IGP synthase glutamine amidotransferase subunit; IGP synthase subunit hisH; ImGP synthase subunit hisH; IGPS subunit hisH

Number of amino acids: Translated: 206; Mature: 205

Protein sequence:

>206_residues
MTAKSVVVLDYGSGNLRSAQRALQRVGAEVEVTADTDAAMTADGLVVPGVGAFAACMAGLRKISGERIIAERVAAGRPVL
GVCVGMQILFACGVEFGVQTPGCGHWPGAVIRLEAPVIPHMGWNVVDSAAGSALFKGLDVDARFYFVHSYAAQRWEGSPD
ALLTWATYRAPFLAAVEDGALAATQFHPEKSGDAGAAVLSNWVDGL

Sequences:

>Translated_206_residues
MTAKSVVVLDYGSGNLRSAQRALQRVGAEVEVTADTDAAMTADGLVVPGVGAFAACMAGLRKISGERIIAERVAAGRPVL
GVCVGMQILFACGVEFGVQTPGCGHWPGAVIRLEAPVIPHMGWNVVDSAAGSALFKGLDVDARFYFVHSYAAQRWEGSPD
ALLTWATYRAPFLAAVEDGALAATQFHPEKSGDAGAAVLSNWVDGL
>Mature_205_residues
TAKSVVVLDYGSGNLRSAQRALQRVGAEVEVTADTDAAMTADGLVVPGVGAFAACMAGLRKISGERIIAERVAAGRPVLG
VCVGMQILFACGVEFGVQTPGCGHWPGAVIRLEAPVIPHMGWNVVDSAAGSALFKGLDVDARFYFVHSYAAQRWEGSPDA
LLTWATYRAPFLAAVEDGALAATQFHPEKSGDAGAAVLSNWVDGL

Specific function: IGPS catalyzes the conversion of PRFAR and glutamine to IGP, AICAR and glutamate. The hisH subunit provides the glutamine amidotransferase activity that produces the ammonia necessary to hisF for the synthesis of IGP and AICAR

COG id: COG0118

COG function: function code E; Glutamine amidotransferase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-1 domain

Homologues:

Organism=Escherichia coli, GI1788334, Length=211, Percent_Identity=39.3364928909953, Blast_Score=127, Evalue=7e-31,
Organism=Saccharomyces cerevisiae, GI6319725, Length=210, Percent_Identity=37.1428571428571, Blast_Score=119, Evalue=5e-28,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): HIS5_MYCBO (P59957)

Other databases:

- EMBL:   BX248339
- RefSeq:   NP_855281.1
- ProteinModelPortal:   P59957
- SMR:   P59957
- EnsemblBacteria:   EBMYCT00000014278
- GeneID:   1092520
- GenomeReviews:   BX248333_GR
- KEGG:   mbo:Mb1628
- GeneTree:   EBGT00050000016176
- HOGENOM:   HBG292341
- OMA:   SVRFAFE
- ProtClustDB:   PRK13146
- BioCyc:   MBOV233413:MB1628-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00278
- InterPro:   IPR017926
- InterPro:   IPR000991
- InterPro:   IPR010139
- InterPro:   IPR016226
- PIRSF:   PIRSF000495
- TIGRFAMs:   TIGR01855

Pfam domain/function: PF00117 GATase

EC number: 2.4.2.-

Molecular weight: Translated: 21446; Mature: 21314

Theoretical pI: Translated: 5.21; Mature: 5.21

Prosite motif: PS51273 GATASE_TYPE_1; PS00442 GATASE_TYPE_I

Important sites: ACT_SITE 83-83 ACT_SITE 187-187 ACT_SITE 189-189

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTAKSVVVLDYGSGNLRSAQRALQRVGAEVEVTADTDAAMTADGLVVPGVGAFAACMAGL
CCCCEEEEEECCCCCHHHHHHHHHHCCCEEEEEECCCCCCCCCCEEECCHHHHHHHHHHH
RKISGERIIAERVAAGRPVLGVCVGMQILFACGVEFGVQTPGCGHWPGAVIRLEAPVIPH
HHHCCCHHHHHHHHCCCCHHHHHHHHHHHHHHCHHHCCCCCCCCCCCCEEEEEECCCCCC
MGWNVVDSAAGSALFKGLDVDARFYFVHSYAAQRWEGSPDALLTWATYRAPFLAAVEDGA
CCCCHHHHHHHHHHHHCCCCCEEEEEEEHHHHHHCCCCCCCEEEEEHHCCCEEEEECCCC
LAATQFHPEKSGDAGAAVLSNWVDGL
EEEEEECCCCCCCHHHHHHHHHHCCC
>Mature Secondary Structure 
TAKSVVVLDYGSGNLRSAQRALQRVGAEVEVTADTDAAMTADGLVVPGVGAFAACMAGL
CCCEEEEEECCCCCHHHHHHHHHHCCCEEEEEECCCCCCCCCCEEECCHHHHHHHHHHH
RKISGERIIAERVAAGRPVLGVCVGMQILFACGVEFGVQTPGCGHWPGAVIRLEAPVIPH
HHHCCCHHHHHHHHCCCCHHHHHHHHHHHHHHCHHHCCCCCCCCCCCCEEEEEECCCCCC
MGWNVVDSAAGSALFKGLDVDARFYFVHSYAAQRWEGSPDALLTWATYRAPFLAAVEDGA
CCCCHHHHHHHHHHHHCCCCCEEEEEEEHHHHHHCCCCCCCEEEEEHHCCCEEEEECCCC
LAATQFHPEKSGDAGAAVLSNWVDGL
EEEEEECCCCCCCHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Transferases; Glycosyltransferases; Pentosyltransferases [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12788972