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The map label for this gene is alkA [H]

Identifier: 121637247

GI number: 121637247

Start: 1504142

End: 1505632

Strand: Reverse

Name: alkA [H]

Synonym: BCG_1378c

Alternate gene names: 121637247

Gene position: 1505632-1504142 (Counterclockwise)

Preceding gene: 121637248

Following gene: 121637246

Centisome position: 34.42

GC content: 68.34

Gene sequence:

>1491_bases
GTGCACGACGACTTCGAACGCTGCTACCGGGCGGTCCAGTCCAAAGACGCCCGGTTCGACGGCTGGTTCGTCGTCGCGGT
TTTGACCACCGGTGTCTACTGCCGGCCGAGTTGCCCCGTCCGGCCACCGTTCGCGCGCAATGTCCGGTTCCTGCCGACTG
CGGCGGCCGCTCAGGGGGAGGGATTCCGGGCCTGCAAACGGTGCCGCCCCGACGCCTCGCCTGGGTCTCCGGAATGGAAT
GTGCGTAGTGACGTCGTGGCGCGGGCGATGCGGCTGATTGCCGACGGAACGGTGGACCGCGACGGTGTCAGCGGCCTCGC
GGCCCAGCTCGGTTACACCATTCGCCAGCTGGAGCGGCTGTTGCAGGCCGTGGTCGGCGCCGGTCCGCTCGCGTTGGCCC
GCGCCCAACGCATGCAGACCGCCCGGGTGCTGATCGAGACCACGAACCTGCCGTTCGGCGATGTCGCATTCGCCGCCGGG
TTTTCCAGCATCCGTCAGTTCAACGACACCGTTCGCCTGGCGTGCGACGGCACACCGACGGCATTGCGTGCGCGCGCGGC
CGCCCGATTCGAGTCTGCCACCGCATCAGCGGGCACGGTGTCGCTGCGGCTACCCGTCCGTGCACCATTCGCCTTCGAGG
GTGTTTTCGGCCATCTGGCCGCCACCGCGGTGCCGGGTTGCGAAGAGGTCCGCGATGGTGCGTACCGACGCACGCTACGG
CTCCCATGGGGCAACGGCATCGTCAGCCTGACGCCGGCACCCGATCATGTGCGCTGCCTGCTTGTGCTCGATGATTTCCG
CGACCTAATGACGGCCACTGCACGTTGCCGACGGCTGCTGGACCTCGACGCCGATCCCGAAGCGATCGTCGAGGCGCTGG
GCGCCGATCCGGATCTGCGCGCAGTGGTGGGCAAGGCACCCGGGCAACGCATTCCCCGCACAGTCGACGAGGCAGAATTC
GCCGTGCGGGCGGTCCTCGCCCAACAGGTATCGACGAAGGCCGCAAGCACTCACGCGGGCCGACTGGTCGCCGCCTACGG
ACGGCCGGTCCACGATCGCCACGGCGCTTTGACCCACACCTTCCCGTCGATCGAGCAGCTCGCTGAGATCGATCCCGGCC
ATCTGGCCGTCCCCAAGGCGCGTCAAAGGACCATAAACGCGCTCGTCGCCAGCCTTGCCGACAAAAGTCTGGTCCTGGAC
GCCGGATGTGACTGGCAACGCGCCCGCGGGCAGTTGCTAGCGCTGCCCGGAGTGGGCCCCTGGACCGCGGAGGTCATCGC
CATGCGCGGCCTCGGTGACCCGGACGCCTTTCCGGCCAGTGATCTCGGCCTGCGGCTGGCCGCCAAAAAGCTGGGCCTGC
CTGCACAACGACGAGCCCTGACGGTGCACAGCGCTCGCTGGCGCCCCTGGCGCTCCTATGCCACCCAGCACCTGTGGACC
ACCCTGGAACATCCGGTAAACCAATGGCCACCGCAGGAGAAGATCGCATGA

Upstream 100 bases:

>100_bases
TGACCAAGCCGCGTAGGTAACCTGCCCGAACCCACGACGACCCCATCACAATGTCGTTTTTCCGCCAGTCATGTCGGTGG
GCGGGTGTAATTGTTGAAGG

Downstream 100 bases:

>100_bases
TTCACTACCGCACCATCGATAGCCCCATCGGGCCATTAACCCTGGCCGGGCATGGCTCGGTGTTGACGAACCTGCGGATG
CTCGAGCAGACGTATGAGCC

Product: putative ada regulatory protein alkA

Products: NA

Alternate protein names: Regulatory protein of adaptative response; Methylated-DNA--protein-cysteine methyltransferase; O-6-methylguanine-DNA alkyltransferase [H]

Number of amino acids: Translated: 496; Mature: 496

Protein sequence:

>496_residues
MHDDFERCYRAVQSKDARFDGWFVVAVLTTGVYCRPSCPVRPPFARNVRFLPTAAAAQGEGFRACKRCRPDASPGSPEWN
VRSDVVARAMRLIADGTVDRDGVSGLAAQLGYTIRQLERLLQAVVGAGPLALARAQRMQTARVLIETTNLPFGDVAFAAG
FSSIRQFNDTVRLACDGTPTALRARAAARFESATASAGTVSLRLPVRAPFAFEGVFGHLAATAVPGCEEVRDGAYRRTLR
LPWGNGIVSLTPAPDHVRCLLVLDDFRDLMTATARCRRLLDLDADPEAIVEALGADPDLRAVVGKAPGQRIPRTVDEAEF
AVRAVLAQQVSTKAASTHAGRLVAAYGRPVHDRHGALTHTFPSIEQLAEIDPGHLAVPKARQRTINALVASLADKSLVLD
AGCDWQRARGQLLALPGVGPWTAEVIAMRGLGDPDAFPASDLGLRLAAKKLGLPAQRRALTVHSARWRPWRSYATQHLWT
TLEHPVNQWPPQEKIA

Sequences:

>Translated_496_residues
MHDDFERCYRAVQSKDARFDGWFVVAVLTTGVYCRPSCPVRPPFARNVRFLPTAAAAQGEGFRACKRCRPDASPGSPEWN
VRSDVVARAMRLIADGTVDRDGVSGLAAQLGYTIRQLERLLQAVVGAGPLALARAQRMQTARVLIETTNLPFGDVAFAAG
FSSIRQFNDTVRLACDGTPTALRARAAARFESATASAGTVSLRLPVRAPFAFEGVFGHLAATAVPGCEEVRDGAYRRTLR
LPWGNGIVSLTPAPDHVRCLLVLDDFRDLMTATARCRRLLDLDADPEAIVEALGADPDLRAVVGKAPGQRIPRTVDEAEF
AVRAVLAQQVSTKAASTHAGRLVAAYGRPVHDRHGALTHTFPSIEQLAEIDPGHLAVPKARQRTINALVASLADKSLVLD
AGCDWQRARGQLLALPGVGPWTAEVIAMRGLGDPDAFPASDLGLRLAAKKLGLPAQRRALTVHSARWRPWRSYATQHLWT
TLEHPVNQWPPQEKIA
>Mature_496_residues
MHDDFERCYRAVQSKDARFDGWFVVAVLTTGVYCRPSCPVRPPFARNVRFLPTAAAAQGEGFRACKRCRPDASPGSPEWN
VRSDVVARAMRLIADGTVDRDGVSGLAAQLGYTIRQLERLLQAVVGAGPLALARAQRMQTARVLIETTNLPFGDVAFAAG
FSSIRQFNDTVRLACDGTPTALRARAAARFESATASAGTVSLRLPVRAPFAFEGVFGHLAATAVPGCEEVRDGAYRRTLR
LPWGNGIVSLTPAPDHVRCLLVLDDFRDLMTATARCRRLLDLDADPEAIVEALGADPDLRAVVGKAPGQRIPRTVDEAEF
AVRAVLAQQVSTKAASTHAGRLVAAYGRPVHDRHGALTHTFPSIEQLAEIDPGHLAVPKARQRTINALVASLADKSLVLD
AGCDWQRARGQLLALPGVGPWTAEVIAMRGLGDPDAFPASDLGLRLAAKKLGLPAQRRALTVHSARWRPWRSYATQHLWT
TLEHPVNQWPPQEKIA

Specific function: The methylated ADA protein acts as a positive regulator of its own synthesis, as well as that of other proteins. The transcription-activating function of the ADA protein resides in its N-terminus [H]

COG id: COG0122

COG function: function code L; 3-methyladenine DNA glycosylase/8-oxoguanine DNA glycosylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH araC/xylS-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1788383, Length=293, Percent_Identity=32.7645051194539, Blast_Score=115, Evalue=7e-27,
Organism=Escherichia coli, GI1788542, Length=166, Percent_Identity=32.5301204819277, Blast_Score=82, Evalue=7e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004026
- InterPro:   IPR010316
- InterPro:   IPR011257
- InterPro:   IPR003265
- InterPro:   IPR003583
- InterPro:   IPR009057
- InterPro:   IPR012287
- InterPro:   IPR018062
- InterPro:   IPR018060
- InterPro:   IPR023170
- InterPro:   IPR012294 [H]

Pfam domain/function: PF02805 Ada_Zn_binding; PF06029 AlkA_N; PF00730 HhH-GPD [H]

EC number: =2.1.1.63 [H]

Molecular weight: Translated: 53729; Mature: 53729

Theoretical pI: Translated: 9.74; Mature: 9.74

Prosite motif: PS00041 HTH_ARAC_FAMILY_1 ; PS01124 HTH_ARAC_FAMILY_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHDDFERCYRAVQSKDARFDGWFVVAVLTTGVYCRPSCPVRPPFARNVRFLPTAAAAQGE
CCCHHHHHHHHHHCCCCCCCCEEEEEHHHCCCEECCCCCCCCCHHCCEEECCCHHHCCCC
GFRACKRCRPDASPGSPEWNVRSDVVARAMRLIADGTVDRDGVSGLAAQLGYTIRQLERL
CHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHH
LQAVVGAGPLALARAQRMQTARVLIETTNLPFGDVAFAAGFSSIRQFNDTVRLACDGTPT
HHHHHCCCHHHHHHHHHHHHHHEEEEECCCCCCHHHHHHHHHHHHHCCCCEEEEECCCCH
ALRARAAARFESATASAGTVSLRLPVRAPFAFEGVFGHLAATAVPGCEEVRDGAYRRTLR
HHHHHHHHHHHHCCCCCCEEEEEECCCCCCHHHHHHHHHHHHCCCCHHHHHCCHHHEEEE
LPWGNGIVSLTPAPDHVRCLLVLDDFRDLMTATARCRRLLDLDADPEAIVEALGADPDLR
CCCCCCEEEECCCCCCEEEEEEHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHCCCCCHH
AVVGKAPGQRIPRTVDEAEFAVRAVLAQQVSTKAASTHAGRLVAAYGRPVHDRHGALTHT
HHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEECCCCCCCCCCCCCCC
FPSIEQLAEIDPGHLAVPKARQRTINALVASLADKSLVLDAGCDWQRARGQLLALPGVGP
CCCHHHHHCCCCCCEECCHHHHHHHHHHHHHHCCCCEEEECCCCHHHCCCCEEECCCCCC
WTAEVIAMRGLGDPDAFPASDLGLRLAAKKLGLPAQRRALTVHSARWRPWRSYATQHLWT
HHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHCCCHHHHEEEEECCCCCHHHHHHHHHHHH
TLEHPVNQWPPQEKIA
HHHHHHHCCCCHHCCC
>Mature Secondary Structure
MHDDFERCYRAVQSKDARFDGWFVVAVLTTGVYCRPSCPVRPPFARNVRFLPTAAAAQGE
CCCHHHHHHHHHHCCCCCCCCEEEEEHHHCCCEECCCCCCCCCHHCCEEECCCHHHCCCC
GFRACKRCRPDASPGSPEWNVRSDVVARAMRLIADGTVDRDGVSGLAAQLGYTIRQLERL
CHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHH
LQAVVGAGPLALARAQRMQTARVLIETTNLPFGDVAFAAGFSSIRQFNDTVRLACDGTPT
HHHHHCCCHHHHHHHHHHHHHHEEEEECCCCCCHHHHHHHHHHHHHCCCCEEEEECCCCH
ALRARAAARFESATASAGTVSLRLPVRAPFAFEGVFGHLAATAVPGCEEVRDGAYRRTLR
HHHHHHHHHHHHCCCCCCEEEEEECCCCCCHHHHHHHHHHHHCCCCHHHHHCCHHHEEEE
LPWGNGIVSLTPAPDHVRCLLVLDDFRDLMTATARCRRLLDLDADPEAIVEALGADPDLR
CCCCCCEEEECCCCCCEEEEEEHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHCCCCCHH
AVVGKAPGQRIPRTVDEAEFAVRAVLAQQVSTKAASTHAGRLVAAYGRPVHDRHGALTHT
HHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEECCCCCCCCCCCCCCC
FPSIEQLAEIDPGHLAVPKARQRTINALVASLADKSLVLDAGCDWQRARGQLLALPGVGP
CCCHHHHHCCCCCCEECCHHHHHHHHHHHHHHCCCCEEEECCCCHHHCCCCEEECCCCCC
WTAEVIAMRGLGDPDAFPASDLGLRLAAKKLGLPAQRRALTVHSARWRPWRSYATQHLWT
HHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHCCCHHHHEEEEECCCCCHHHHHHHHHHHH
TLEHPVNQWPPQEKIA
HHHHHHHCCCCHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9634230; 12218036 [H]