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The map label for this gene is ogt

Identifier: 121637246

GI number: 121637246

Start: 1503648

End: 1504145

Strand: Reverse

Name: ogt

Synonym: BCG_1377c

Alternate gene names: 121637246

Gene position: 1504145-1503648 (Counterclockwise)

Preceding gene: 121637247

Following gene: 121637244

Centisome position: 34.38

GC content: 61.85

Gene sequence:

>498_bases
ATGATTCACTACCGCACCATCGATAGCCCCATCGGGCCATTAACCCTGGCCGGGCATGGCTCGGTGTTGACGAACCTGCG
GATGCTCGAGCAGACGTATGAGCCAAGCCGCACACACTGGACACCCGACCCCGGCGCATTTTCTGGCGCTGTCGACCAAC
TCAACGCTTATTTCGCCGGCGAGCTCACCGAATTCGATGTGGAACTTGACCTCCGGGGAACCGACTTTCAGCAACGAGTA
TGGAAAGCATTGCTGACAATCCCGTACGGGGAAACCCGGTCCTACGGGGAAATCGCCGACCAGATCGGCGCCCCCGGCGC
CGCACGCGCCGTGGGATTGGCCAACGGCCACAATCCCATCGCCATCATCGTCCCGTGCCACCGCGTGATCGGCGCCAGCG
GAAAGCTCACCGGGTACGGCGGTGGAATCAACCGGAAACGAGCTCTGCTCGAGTTGGAGAAAAGCCGGGCGCCCGCAGAC
TTGACGCTCTTCGACTGA

Upstream 100 bases:

>100_bases
ACAGCGCTCGCTGGCGCCCCTGGCGCTCCTATGCCACCCAGCACCTGTGGACCACCCTGGAACATCCGGTAAACCAATGG
CCACCGCAGGAGAAGATCGC

Downstream 100 bases:

>100_bases
GCGCCCCCGCCCGCGAGGGTATCGTCATTGCGAAAATCGAAGCCATAATTCGCCCGCTCGCGAGCGGCTGAGTCGATATA
AACATACAAAAACACCACCG

Product: putative methylated-DNA--protein-cysteine methyltransferase ogt

Products: NA

Alternate protein names: 6-O-methylguanine-DNA methyltransferase; MGMT; O-6-methylguanine-DNA-alkyltransferase

Number of amino acids: Translated: 165; Mature: 165

Protein sequence:

>165_residues
MIHYRTIDSPIGPLTLAGHGSVLTNLRMLEQTYEPSRTHWTPDPGAFSGAVDQLNAYFAGELTEFDVELDLRGTDFQQRV
WKALLTIPYGETRSYGEIADQIGAPGAARAVGLANGHNPIAIIVPCHRVIGASGKLTGYGGGINRKRALLELEKSRAPAD
LTLFD

Sequences:

>Translated_165_residues
MIHYRTIDSPIGPLTLAGHGSVLTNLRMLEQTYEPSRTHWTPDPGAFSGAVDQLNAYFAGELTEFDVELDLRGTDFQQRV
WKALLTIPYGETRSYGEIADQIGAPGAARAVGLANGHNPIAIIVPCHRVIGASGKLTGYGGGINRKRALLELEKSRAPAD
LTLFD
>Mature_165_residues
MIHYRTIDSPIGPLTLAGHGSVLTNLRMLEQTYEPSRTHWTPDPGAFSGAVDQLNAYFAGELTEFDVELDLRGTDFQQRV
WKALLTIPYGETRSYGEIADQIGAPGAARAVGLANGHNPIAIIVPCHRVIGASGKLTGYGGGINRKRALLELEKSRAPAD
LTLFD

Specific function: Involved in the cellular defense against the biological effects of O6-methylguanine (O6-MeG) in DNA. Repairs alkylated guanine in DNA by stoichiometrically transferring the alkyl group at the O-6 position to a cysteine residue in the enzyme. This is a sui

COG id: COG0350

COG function: function code L; Methylated DNA-protein cysteine methyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MGMT family

Homologues:

Organism=Homo sapiens, GI197304670, Length=166, Percent_Identity=36.7469879518072, Blast_Score=87, Evalue=6e-18,
Organism=Escherichia coli, GI1787596, Length=112, Percent_Identity=55.3571428571429, Blast_Score=122, Evalue=1e-29,
Organism=Escherichia coli, GI1788542, Length=107, Percent_Identity=48.5981308411215, Blast_Score=103, Evalue=6e-24,
Organism=Caenorhabditis elegans, GI115533068, Length=80, Percent_Identity=55, Blast_Score=90, Evalue=6e-19,
Organism=Caenorhabditis elegans, GI115533070, Length=80, Percent_Identity=55, Blast_Score=89, Evalue=7e-19,
Organism=Saccharomyces cerevisiae, GI6320001, Length=87, Percent_Identity=49.4252873563218, Blast_Score=96, Evalue=3e-21,
Organism=Drosophila melanogaster, GI17137554, Length=131, Percent_Identity=36.6412213740458, Blast_Score=69, Evalue=1e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): OGT_MYCBO (P0A697)

Other databases:

- EMBL:   BX248338
- RefSeq:   NP_855003.1
- ProteinModelPortal:   P0A697
- SMR:   P0A697
- EnsemblBacteria:   EBMYCT00000016082
- GeneID:   1090642
- GenomeReviews:   BX248333_GR
- KEGG:   mbo:Mb1349c
- GeneTree:   EBGT00050000016345
- HOGENOM:   HBG752398
- OMA:   THARAVG
- ProtClustDB:   CLSK791095
- BioCyc:   MBOV233413:MB1349C-MONOMER
- BRENDA:   2.1.1.63
- InterPro:   IPR001497
- InterPro:   IPR014048
- InterPro:   IPR008332
- InterPro:   IPR011991
- Gene3D:   G3DSA:1.10.10.10
- PANTHER:   PTHR10815
- TIGRFAMs:   TIGR00589

Pfam domain/function: PF01035 DNA_binding_1; PF02870 Methyltransf_1N; SSF46767 MethylDNA_cys_mtrans_DNA_bd

EC number: =2.1.1.63

Molecular weight: Translated: 17858; Mature: 17858

Theoretical pI: Translated: 6.35; Mature: 6.35

Prosite motif: PS00374 MGMT

Important sites: ACT_SITE 126-126

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
1.8 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIHYRTIDSPIGPLTLAGHGSVLTNLRMLEQTYEPSRTHWTPDPGAFSGAVDQLNAYFAG
CCEEEECCCCCCCEEECCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHCC
ELTEFDVELDLRGTDFQQRVWKALLTIPYGETRSYGEIADQIGAPGAARAVGLANGHNPI
CEEEEEEEEEECCCCHHHHHHHHHHHCCCCCCCCHHHHHHHHCCCCCHHEEECCCCCCCE
AIIVPCHRVIGASGKLTGYGGGINRKRALLELEKSRAPADLTLFD
EEEEECHHHHCCCCCEEEECCCCCCHHHHHHHHHCCCCCCEEECC
>Mature Secondary Structure
MIHYRTIDSPIGPLTLAGHGSVLTNLRMLEQTYEPSRTHWTPDPGAFSGAVDQLNAYFAG
CCEEEECCCCCCCEEECCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHCC
ELTEFDVELDLRGTDFQQRVWKALLTIPYGETRSYGEIADQIGAPGAARAVGLANGHNPI
CEEEEEEEEEECCCCHHHHHHHHHHHCCCCCCCCHHHHHHHHCCCCCHHEEECCCCCCCE
AIIVPCHRVIGASGKLTGYGGGINRKRALLELEKSRAPADLTLFD
EEEEECHHHHCCCCCEEEECCCCCCHHHHHHHHHCCCCCCEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12788972