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The map label for this gene is potI [H]

Identifier: 121610133

GI number: 121610133

Start: 3559302

End: 3560129

Strand: Direct

Name: potI [H]

Synonym: Veis_3192

Alternate gene names: 121610133

Gene position: 3559302-3560129 (Clockwise)

Preceding gene: 121610132

Following gene: 121610135

Centisome position: 63.94

GC content: 61.59

Gene sequence:

>828_bases
ATGCCAAAAATGGATGCCAAACATACCGGTCATATGAGCCGCTCCATGGCCGTCTTCGCCATCGGCTTTTTGTTGTTCCC
GTTGCTCGCGGTGGTGCCCGTGTCGTTCACGTCAAAGCGCTACCTGTCGATGCCTGAGGGCCACTGGTCGCTGCGCCACT
ATGCCGCGTTGTTCAGCAGCAGCGACTGGATGTCCAGCATAGTCCAGAGCGTGCTGGTGGCGGCGTGCGCGAGCGTGATC
GCGACGGTGCTCGCGGTGACATTCAGCGTGGGTGTCTGGTATGTGCGCTCACGCATCGCCAAGGCGATGGTGGCGCTCGT
GCTGGTGCCGATGGTGATTCCGCCGGTGATTTCCGCGATGGTGCTGTATTTCATGGAGACGAAGCTGGGTTCGTGGATGC
CGTCGGCCGGTTATGACACGCTGCCCGGCGTCGTGATCGCGCACATCGTGGTGATTACGCCGTACTGCGTGGTGACGATG
CTCGTCGCGTTGTCCCCACTCGACCGGCGCATCGAGCTTGCCGCGCGCAATCTTGGGGCGGGGCTGGCGCAAACCACGTT
CATGGTGGTGTTGCCGAACCTCAAATTGGCCATTGCGTCGACGTTTTTCCTCGGGCTTGCGCTGTCCTGGGAAGAAATTT
CGGTGACGCTGTTCGTGACCAGCGTCAACGTCAATACCGTGCCGCGACATATCTGGACGGATCTGCGGGACAACATCAGT
CCTGGCGTCGCGGCGATTTCCGTGCTGCTGCTTGCGCTCACGACGGCCGTGCTGCTCGGGCGCATGGCGGTGCAGCATCT
GGCAGCACGCAGGCAAACCACGCACTGA

Upstream 100 bases:

>100_bases
TCGGTGCAGCGTTGAGCGTCGTGCTGCTGGCGTTCATAGGGCTGATGGCCGCGCTCCTGATGTGGGCGAGCCGCGTCAAC
AAACTGGTCCGTTGAAATGG

Downstream 100 bases:

>100_bases
GGAACTCCGTCATGGCAAGTGCGAACGGGGCGGAGATGCGCAGATCGGTGCGCTACCACGCTGTGGTGGGGATCTCTTTC
GCCACGGGTGCGAGTTTGCG

Product: binding-protein-dependent transport systems inner membrane component

Products: ADP; phosphate; putrescine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 275; Mature: 274

Protein sequence:

>275_residues
MPKMDAKHTGHMSRSMAVFAIGFLLFPLLAVVPVSFTSKRYLSMPEGHWSLRHYAALFSSSDWMSSIVQSVLVAACASVI
ATVLAVTFSVGVWYVRSRIAKAMVALVLVPMVIPPVISAMVLYFMETKLGSWMPSAGYDTLPGVVIAHIVVITPYCVVTM
LVALSPLDRRIELAARNLGAGLAQTTFMVVLPNLKLAIASTFFLGLALSWEEISVTLFVTSVNVNTVPRHIWTDLRDNIS
PGVAAISVLLLALTTAVLLGRMAVQHLAARRQTTH

Sequences:

>Translated_275_residues
MPKMDAKHTGHMSRSMAVFAIGFLLFPLLAVVPVSFTSKRYLSMPEGHWSLRHYAALFSSSDWMSSIVQSVLVAACASVI
ATVLAVTFSVGVWYVRSRIAKAMVALVLVPMVIPPVISAMVLYFMETKLGSWMPSAGYDTLPGVVIAHIVVITPYCVVTM
LVALSPLDRRIELAARNLGAGLAQTTFMVVLPNLKLAIASTFFLGLALSWEEISVTLFVTSVNVNTVPRHIWTDLRDNIS
PGVAAISVLLLALTTAVLLGRMAVQHLAARRQTTH
>Mature_274_residues
PKMDAKHTGHMSRSMAVFAIGFLLFPLLAVVPVSFTSKRYLSMPEGHWSLRHYAALFSSSDWMSSIVQSVLVAACASVIA
TVLAVTFSVGVWYVRSRIAKAMVALVLVPMVIPPVISAMVLYFMETKLGSWMPSAGYDTLPGVVIAHIVVITPYCVVTML
VALSPLDRRIELAARNLGAGLAQTTFMVVLPNLKLAIASTFFLGLALSWEEISVTLFVTSVNVNTVPRHIWTDLRDNISP
GVAAISVLLLALTTAVLLGRMAVQHLAARRQTTH

Specific function: Required for the activity of the bacterial periplasmic transport system of putrescine [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ABC transmembrane type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI1787081, Length=234, Percent_Identity=30.3418803418803, Blast_Score=75, Evalue=4e-15,
Organism=Escherichia coli, GI1787368, Length=243, Percent_Identity=25.5144032921811, Blast_Score=69, Evalue=3e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000515 [H]

Pfam domain/function: PF00528 BPD_transp_1 [H]

EC number: NA

Molecular weight: Translated: 29835; Mature: 29704

Theoretical pI: Translated: 10.28; Mature: 10.28

Prosite motif: PS50928 ABC_TM1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
5.1 %Met     (Translated Protein)
5.8 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
4.7 %Met     (Mature Protein)
5.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
PKMDAKHTGHMSRSMAVFAIGFLLFPLLAVVPVSFTSKRYLSMPEGHWSLRHYAALFSS
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHCCCCCCCHHHHHHHHHHCC
SDWMSSIVQSVLVAACASVIATVLAVTFSVGVWYVRSRIAKAMVALVLVPMVIPPVISAM
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VLYFMETKLGSWMPSAGYDTLPGVVIAHIVVITPYCVVTMLVALSPLDRRIELAARNLGA
HHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCC
GLAQTTFMVVLPNLKLAIASTFFLGLALSWEEISVTLFVTSVNVNTVPRHIWTDLRDNIS
HHHHHHHHHHCCCHHHHHHHHHHHHHHCCHHHEEEEEEEEEECCCHHHHHHHHHHHHCCC
PGVAAISVLLLALTTAVLLGRMAVQHLAARRQTTH
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; putrescine [Periplasm]; H2O [C]

Specific reaction: ATP + putrescine [Periplasm] + H2O = ADP + phosphate + putrescine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 12471157 [H]