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The map label for this gene is potB [H]

Identifier: 121610132

GI number: 121610132

Start: 3558460

End: 3559296

Strand: Direct

Name: potB [H]

Synonym: Veis_3191

Alternate gene names: 121610132

Gene position: 3558460-3559296 (Clockwise)

Preceding gene: 121610131

Following gene: 121610133

Centisome position: 63.92

GC content: 59.98

Gene sequence:

>837_bases
ATGAATGCCGTGAACTCCACCGCCTTCAAACGCGCATTGCTGATCGCACCGCTGCCGCTATTGCTGGCGCTGCTCTATGG
TTTGCCGCTGGCCGGTGTGATCGGCTGGAGCGTCACTTTGCCGACGCCTGGCCTGGAGCAGTATCGGCAGGTGGTGACCG
ATCCATCGATTCGGGACATGCTGCTGCGCACGTTGCGGCTGTGCCTGTCCAGCACCGTTCTTGCGGTTGCGATTGGCTAT
CTGCTGTCGTATTGCTGGGTGTTCGCGTCGCGTCCATGGCAGCGTGTGGTGGAAATCGCGGTGTTCGTACCGATGTGGAT
TTCGGTCCTGGTGCGTGCATTCGGCTGGCTGATTGCGCTTGGCAACGAGGGCATTCTCAACACCTTGCTGATGAGCGTTG
GCTTGATTCACGGGCCTGTGCAAATGACGCGCAATGAGTTTGCCGTGGTCGTTGGCATGGTGCATTTCATGGTGCCATAT
GCGGTCTTTCCGCTGTCTTCGAGCATGCGCCAAATCGACCGGCGCACGCTGCTTGCAGCACGCGGTTTGGGCGCCGGCGG
CCTGCGCACCTTCTGGGCCGTGCTGGTGCCGCAGACGCTGCCCGGACTGCTTGGTGCCTTTCTGATCGTGTTCGTGTTCT
GCCTTGGCTTTTTCATCACACCGGCCATTCTTGGCGGCGGACAGACCACCATGATCGCCGAATATGTTTATCTGCAGATG
TTCCAGACCAACAACTGGGGTCTCGGTGCAGCGTTGAGCGTCGTGCTGCTGGCGTTCATAGGGCTGATGGCCGCGCTCCT
GATGTGGGCGAGCCGCGTCAACAAACTGGTCCGTTGA

Upstream 100 bases:

>100_bases
AACCTTGCCAAGCAGATTCGTCTGAACGGTGATTGGTATGCGGACCACTATGCCGCCGCGCTCGAACAATATCTGAGACG
GTTGGCCAAGTAGATGGGTG

Downstream 100 bases:

>100_bases
AATGGATGCCAAAAATGGATGCCAAACATACCGGTCATATGAGCCGCTCCATGGCCGTCTTCGCCATCGGCTTTTTGTTG
TTCCCGTTGCTCGCGGTGGT

Product: binding-protein-dependent transport systems inner membrane component

Products: phosphate; putrescine [Cytoplasm]; ADP; spermidine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 278; Mature: 278

Protein sequence:

>278_residues
MNAVNSTAFKRALLIAPLPLLLALLYGLPLAGVIGWSVTLPTPGLEQYRQVVTDPSIRDMLLRTLRLCLSSTVLAVAIGY
LLSYCWVFASRPWQRVVEIAVFVPMWISVLVRAFGWLIALGNEGILNTLLMSVGLIHGPVQMTRNEFAVVVGMVHFMVPY
AVFPLSSSMRQIDRRTLLAARGLGAGGLRTFWAVLVPQTLPGLLGAFLIVFVFCLGFFITPAILGGGQTTMIAEYVYLQM
FQTNNWGLGAALSVVLLAFIGLMAALLMWASRVNKLVR

Sequences:

>Translated_278_residues
MNAVNSTAFKRALLIAPLPLLLALLYGLPLAGVIGWSVTLPTPGLEQYRQVVTDPSIRDMLLRTLRLCLSSTVLAVAIGY
LLSYCWVFASRPWQRVVEIAVFVPMWISVLVRAFGWLIALGNEGILNTLLMSVGLIHGPVQMTRNEFAVVVGMVHFMVPY
AVFPLSSSMRQIDRRTLLAARGLGAGGLRTFWAVLVPQTLPGLLGAFLIVFVFCLGFFITPAILGGGQTTMIAEYVYLQM
FQTNNWGLGAALSVVLLAFIGLMAALLMWASRVNKLVR
>Mature_278_residues
MNAVNSTAFKRALLIAPLPLLLALLYGLPLAGVIGWSVTLPTPGLEQYRQVVTDPSIRDMLLRTLRLCLSSTVLAVAIGY
LLSYCWVFASRPWQRVVEIAVFVPMWISVLVRAFGWLIALGNEGILNTLLMSVGLIHGPVQMTRNEFAVVVGMVHFMVPY
AVFPLSSSMRQIDRRTLLAARGLGAGGLRTFWAVLVPQTLPGLLGAFLIVFVFCLGFFITPAILGGGQTTMIAEYVYLQM
FQTNNWGLGAALSVVLLAFIGLMAALLMWASRVNKLVR

Specific function: Required for the activity of the bacterial periplasmic transport system of putrescine and spermidine [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ABC transmembrane type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI1787369, Length=238, Percent_Identity=33.1932773109244, Blast_Score=135, Evalue=4e-33,
Organism=Escherichia coli, GI1787080, Length=240, Percent_Identity=31.6666666666667, Blast_Score=129, Evalue=2e-31,
Organism=Escherichia coli, GI1787713, Length=226, Percent_Identity=24.7787610619469, Blast_Score=75, Evalue=6e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000515 [H]

Pfam domain/function: PF00528 BPD_transp_1 [H]

EC number: NA

Molecular weight: Translated: 30414; Mature: 30414

Theoretical pI: Translated: 10.54; Mature: 10.54

Prosite motif: PS50928 ABC_TM1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
4.3 %Met     (Translated Protein)
5.4 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
4.3 %Met     (Mature Protein)
5.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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HHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure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HHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: putrescine [Periplasm]; H2O; ATP; spermidine [Periplasm] [C]

Specific reaction: ATP + putrescine [Periplasm] + H2O = ADP + phosphate + putrescine [Cytoplasm] ATP + spermidine [Periplasm] + H2O = ADP + phosphate + spermidine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 11677608; 12644504 [H]