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The map label for this gene is tnpA [H]

Identifier: 121610050

GI number: 121610050

Start: 3455023

End: 3456282

Strand: Direct

Name: tnpA [H]

Synonym: Veis_3107

Alternate gene names: 121610050

Gene position: 3455023-3456282 (Clockwise)

Preceding gene: 121610049

Following gene: 121610052

Centisome position: 62.07

GC content: 67.3

Gene sequence:

>1260_bases
ATGGAACGGATGAGCTGGGCACAAGAGGAGTTCGCGGACCTGGACCTGGGCGACAAGCGCCTGAACCGGCGGCAGGTGAA
ACTGGCCGAGACCTTCAGCCGGCAGCCCACACCCAGCATCCCGGCTGCCTGCGGGGGCTGGGGCGACACCCGCGGTGCCT
ACCGCTTCTTTGCGCACGAGGACATTGACTGGCAGGACATCCTCAAGCCGCACTGGCAGGCCAGCGCCCAGCGCATGGCC
GAGCACCCGGTGGTGCTGTGCCTGCAGGACACGACGGAGCTGGACTTCAACGGCCAGGGCATTGCCGGGCTGGGCCGGCT
GAGCTACGACGCGCAGCGCGGGATGTACCTGCATCCGACCTACGCGATCACGCCCGATCGCGAACCGCTGGGCGTGATCG
ATGCGTGGATGTGGGCGCGTTCGGCCAAGAAGGGGCAGGCTGCGAGCGATGAGGTGCTGGAGAGCACGCGCTGGATCGAA
GGCTACGAGCGCATCGCCGAGCAGGCGGCCAGCCTGCCTGGAACCCGGCTGGTCTATGTGGCCGACCGCGAAAGCGACCT
GCTGGCGCTGATGCGCCGCGCTGCCGAATTGGGCCATCCGGCCGACTGGCTGGTGCGCGCCAAGCACAACCGGGTTCTGC
CCGAAGACGGCAAGCTGTGGGCCACAGTGACAAGCGGCCACCCTCTGGGCCAGATCGTCTTCACCCACGCGCCGCGCGGC
CAGCGCGCCCGGCGGGTGCACCAGACGGTGTGGGCCAAGTCGGTGCGGCTGGCCCAGGGCGTCACCGTGACCTGTGTGAT
CGCTCGCGAAGAACACCCGCCGAGCGGCGTCAAACCCATCGAATGGCGCTTGCTGAGCAACCGCCCAGCCGGCGACTTGC
AGGCAGCCACGCAGTTGATCGAGTGGTACCGCGCCCGCTGGGAGATCGAACTGTTCTTTCACGTCGTCAAGAACGGCTGC
CGGATCGAGGCACTGCAATTGAGCCGCATCGAGCGGCTCGAGCGCGCCATCGCCCTGTACCTGGTGATAGCCTGGCGCAT
CGCGCGCTTGATGCGCCTGGGGCGCAATGGCCCGGATCTACCCGCCGAACTGTTCTTCGAGCCCGACGAATGGCGCGGCG
TGCACTTCCTGCTCAAAAAGCCCATCCCCAAGCAGGCGCCGCGCCTGAACACCGTCATCCGGATGGTTGCCACGCTGGGC
GGCTTTCTGGGGCGCAAGTGCGATGGCGAGCCCGGCGTGAAGACGTTAGAGCATATTTGA

Upstream 100 bases:

>100_bases
AGACCCGCTGCCATACGCCCGACTCGCCCCAGCGCTTGAACCATCCGATTGCACACATCTTGAAGATTCGGCGGTAAGCT
GTTGATTTTTCGAAGAGAAG

Downstream 100 bases:

>100_bases
CTTAAAACCAAACAATTCATGATATGATGCGGCGTCTTTTTTCTGAAGGAGTATGCAATGCAAGCGTATTCGCAGGATTT
GCGAGATAGGGTGCTGAAGG

Product: transposase, IS4 family protein

Products: NA

Alternate protein names: Tnp [H]

Number of amino acids: Translated: 419; Mature: 419

Protein sequence:

>419_residues
MERMSWAQEEFADLDLGDKRLNRRQVKLAETFSRQPTPSIPAACGGWGDTRGAYRFFAHEDIDWQDILKPHWQASAQRMA
EHPVVLCLQDTTELDFNGQGIAGLGRLSYDAQRGMYLHPTYAITPDREPLGVIDAWMWARSAKKGQAASDEVLESTRWIE
GYERIAEQAASLPGTRLVYVADRESDLLALMRRAAELGHPADWLVRAKHNRVLPEDGKLWATVTSGHPLGQIVFTHAPRG
QRARRVHQTVWAKSVRLAQGVTVTCVIAREEHPPSGVKPIEWRLLSNRPAGDLQAATQLIEWYRARWEIELFFHVVKNGC
RIEALQLSRIERLERAIALYLVIAWRIARLMRLGRNGPDLPAELFFEPDEWRGVHFLLKKPIPKQAPRLNTVIRMVATLG
GFLGRKCDGEPGVKTLEHI

Sequences:

>Translated_419_residues
MERMSWAQEEFADLDLGDKRLNRRQVKLAETFSRQPTPSIPAACGGWGDTRGAYRFFAHEDIDWQDILKPHWQASAQRMA
EHPVVLCLQDTTELDFNGQGIAGLGRLSYDAQRGMYLHPTYAITPDREPLGVIDAWMWARSAKKGQAASDEVLESTRWIE
GYERIAEQAASLPGTRLVYVADRESDLLALMRRAAELGHPADWLVRAKHNRVLPEDGKLWATVTSGHPLGQIVFTHAPRG
QRARRVHQTVWAKSVRLAQGVTVTCVIAREEHPPSGVKPIEWRLLSNRPAGDLQAATQLIEWYRARWEIELFFHVVKNGC
RIEALQLSRIERLERAIALYLVIAWRIARLMRLGRNGPDLPAELFFEPDEWRGVHFLLKKPIPKQAPRLNTVIRMVATLG
GFLGRKCDGEPGVKTLEHI
>Mature_419_residues
MERMSWAQEEFADLDLGDKRLNRRQVKLAETFSRQPTPSIPAACGGWGDTRGAYRFFAHEDIDWQDILKPHWQASAQRMA
EHPVVLCLQDTTELDFNGQGIAGLGRLSYDAQRGMYLHPTYAITPDREPLGVIDAWMWARSAKKGQAASDEVLESTRWIE
GYERIAEQAASLPGTRLVYVADRESDLLALMRRAAELGHPADWLVRAKHNRVLPEDGKLWATVTSGHPLGQIVFTHAPRG
QRARRVHQTVWAKSVRLAQGVTVTCVIAREEHPPSGVKPIEWRLLSNRPAGDLQAATQLIEWYRARWEIELFFHVVKNGC
RIEALQLSRIERLERAIALYLVIAWRIARLMRLGRNGPDLPAELFFEPDEWRGVHFLLKKPIPKQAPRLNTVIRMVATLG
GFLGRKCDGEPGVKTLEHI

Specific function: Mediates transposition of transposon Tn5 by a 'cut and paste' mechanism. First, the monomeric transposase binds the 19 bp inverted DNA repeats flanking the transposon. Then, dimerization of the DNA-bound transposase creates a synaptic DNA complex. After n

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the transposase 11 family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012337
- InterPro:   IPR002559
- InterPro:   IPR003201
- InterPro:   IPR014737
- InterPro:   IPR014736
- InterPro:   IPR014735 [H]

Pfam domain/function: PF01609 Transposase_11; PF02281 Transposase_Tn5 [H]

EC number: NA

Molecular weight: Translated: 47596; Mature: 47596

Theoretical pI: Translated: 9.15; Mature: 9.15

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MERMSWAQEEFADLDLGDKRLNRRQVKLAETFSRQPTPSIPAACGGWGDTRGAYRFFAHE
CCCCHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCEEEEECC
DIDWQDILKPHWQASAQRMAEHPVVLCLQDTTELDFNGQGIAGLGRLSYDAQRGMYLHPT
CCCHHHHHCHHHHHHHHHHHCCCEEEEEECCCCCCCCCCCCCCCCCCCHHHHCCEEECCE
YAITPDREPLGVIDAWMWARSAKKGQAASDEVLESTRWIEGYERIAEQAASLPGTRLVYV
EEECCCCCCCHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEE
ADRESDLLALMRRAAELGHPADWLVRAKHNRVLPEDGKLWATVTSGHPLGQIVFTHAPRG
ECCCHHHHHHHHHHHHCCCCHHHHHHHHCCCCCCCCCCEEEEEECCCCCEEEEEEECCCC
QRARRVHQTVWAKSVRLAQGVTVTCVIAREEHPPSGVKPIEWRLLSNRPAGDLQAATQLI
HHHHHHHHHHHHHHHHHHCCCEEEEEEEECCCCCCCCCCEEEEEECCCCCCHHHHHHHHH
EWYRARWEIELFFHVVKNGCRIEALQLSRIERLERAIALYLVIAWRIARLMRLGRNGPDL
HHHHHHHHHHHHHHHHHCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
PAELFFEPDEWRGVHFLLKKPIPKQAPRLNTVIRMVATLGGFLGRKCDGEPGVKTLEHI
CHHHEECCCCCCCEEEEECCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHCC
>Mature Secondary Structure
MERMSWAQEEFADLDLGDKRLNRRQVKLAETFSRQPTPSIPAACGGWGDTRGAYRFFAHE
CCCCHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCEEEEECC
DIDWQDILKPHWQASAQRMAEHPVVLCLQDTTELDFNGQGIAGLGRLSYDAQRGMYLHPT
CCCHHHHHCHHHHHHHHHHHCCCEEEEEECCCCCCCCCCCCCCCCCCCHHHHCCEEECCE
YAITPDREPLGVIDAWMWARSAKKGQAASDEVLESTRWIEGYERIAEQAASLPGTRLVYV
EEECCCCCCCHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEE
ADRESDLLALMRRAAELGHPADWLVRAKHNRVLPEDGKLWATVTSGHPLGQIVFTHAPRG
ECCCHHHHHHHHHHHHCCCCHHHHHHHHCCCCCCCCCCEEEEEECCCCCEEEEEEECCCC
QRARRVHQTVWAKSVRLAQGVTVTCVIAREEHPPSGVKPIEWRLLSNRPAGDLQAATQLI
HHHHHHHHHHHHHHHHHHCCCEEEEEEEECCCCCCCCCCEEEEEECCCCCCHHHHHHHHH
EWYRARWEIELFFHVVKNGCRIEALQLSRIERLERAIALYLVIAWRIARLMRLGRNGPDL
HHHHHHHHHHHHHHHHHCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
PAELFFEPDEWRGVHFLLKKPIPKQAPRLNTVIRMVATLGGFLGRKCDGEPGVKTLEHI
CHHHEECCCCCCCEEEEECCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 6260374; 6271452; 7867940; 6291786; 10884228; 12367522; 11896402 [H]