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The map label for this gene is tnpA [H]

Identifier: 121610049

GI number: 121610049

Start: 3453337

End: 3454656

Strand: Direct

Name: tnpA [H]

Synonym: Veis_3105

Alternate gene names: 121610049

Gene position: 3453337-3454656 (Clockwise)

Preceding gene: 121610047

Following gene: 121610050

Centisome position: 62.04

GC content: 67.58

Gene sequence:

>1320_bases
ATGGAACGGATGAGCTGGGCACAAGAGGAGTTCGCGGACCTGGACCTGGGCGACAAGCGCCTGAACCGGCGGCAGGTGAA
ACTGGCCGAGACCTTCAGCCGGCAGCCCACACCCAGCATCCCGGCTGCCTGCGGGGGCTGGGGCGACACCCGCGGTGCCT
ACCGCTTCTTTGCGCACGAGGACATTGACTGGCAGGACATCCTCAAGCCGCACTGGCAGGCCAGCGCCCAGCGCATGGCC
GAGCACCCGGTGGTGCTGTGCCTGCAGGACACGACGGAGCTGGACTTCAACGGCCAGGGCATTGCCGGGCTGGGCCGGCT
GAGCTACGACGCGCAGCGCGGGATGTACCTGCATCCGACCTACGCGATCACGCCCGATCGCGAACCGCTGGGCGTGATCG
ATGCGTGGATGTGGGCGCGTTCGGCCAAGAAGGGGCAGGCTGCGAGCGATGAGGTGCTGGAGAGCACGCGCTGGATCGAA
GGCTACGAGCGCATCGCCGAGCAGGCGGCCAGCCTGCCTGGAACCCGGCTGGTCTATGTGGCCGACCGCGAAAGCGACCT
GCTGGCGCTGATGCGCCGCGCTGCCGAATTGGGCCATCCGGCCGACTGGCTGGTGCGCGCCAAGCACAACCGGGTTCTGC
CCGAAGACGGCAAGCTGTGGGCCACAGTGACAAGCGGCCACCCTCTGGGCCAGATCGTCTTCACCCACGCGCCGCGCGGC
CAGCGCGCCCGGCGGGTGCACCAGACGGTGTGGGCCAAGTCGGTGCGGCTGGCCCAGGGCGTCACCGTGACCTGTGTGAT
CGCTCGCGAAGAACACCCGCCGAGCGGCGTCAAACCCATCGAATGGCGCTTGCTGAGCAACCGCCCAGCCGGCGACTTGC
AGGCAGCCACGCAGTTGATCGAGTGGTACCGCGCCCGCTGGGAGATCGAACTGTTCTTTCACGTCGTCAAGAACGGCTGC
CGGATCGAGGCACTGCAATTGAGCCGCATCGAGCGGCTCGAGCGCGCCATCGCCCTGTACCTGGTGATAGCCTGGCGCCT
CGCGCGCTTGATGCGCCTGGGGCGCAATGGCCCGGATCTACCCGCCGAACTGTTCTTCGAGCCCGACGAATGGCGCGGCG
TGCACTTCCTGCTCAAAAAGCCCATCCCCAAGCAGGCGCCGCGCCTGAACACCGTCATCCGGATGGTTGCCACGCTGGGC
GGCTTTCTGGGGCGCAAGTGCGATGGCGAGCCCGGCGTGAAGACGTTATGGATCGGCATGCAGCGCGTGGCCGACTTCGC
TGCCGGACTGGACTACGCCAGGGAAATCCAGGCGCTGTGA

Upstream 100 bases:

>100_bases
ATCGTAGCCCTTGTCAGCCAGTACCGCATCGGCTTTCAAGCCATCCGATTGCACACATCTTGAAGATTCGGCGGTAAGCT
GTTGATTTTTCGAAGAGAAG

Downstream 100 bases:

>100_bases
GATGTGTGCAATCGGATGCTTTCAAGCCTTCCAACAGCGACTTGGCTTGTGTAATATCGGCTACCTCGCCACCCGTGAGC
AGCCAACGCACTGGGCTACC

Product: transposase, IS4 family protein

Products: NA

Alternate protein names: Tnp [H]

Number of amino acids: Translated: 439; Mature: 439

Protein sequence:

>439_residues
MERMSWAQEEFADLDLGDKRLNRRQVKLAETFSRQPTPSIPAACGGWGDTRGAYRFFAHEDIDWQDILKPHWQASAQRMA
EHPVVLCLQDTTELDFNGQGIAGLGRLSYDAQRGMYLHPTYAITPDREPLGVIDAWMWARSAKKGQAASDEVLESTRWIE
GYERIAEQAASLPGTRLVYVADRESDLLALMRRAAELGHPADWLVRAKHNRVLPEDGKLWATVTSGHPLGQIVFTHAPRG
QRARRVHQTVWAKSVRLAQGVTVTCVIAREEHPPSGVKPIEWRLLSNRPAGDLQAATQLIEWYRARWEIELFFHVVKNGC
RIEALQLSRIERLERAIALYLVIAWRLARLMRLGRNGPDLPAELFFEPDEWRGVHFLLKKPIPKQAPRLNTVIRMVATLG
GFLGRKCDGEPGVKTLWIGMQRVADFAAGLDYAREIQAL

Sequences:

>Translated_439_residues
MERMSWAQEEFADLDLGDKRLNRRQVKLAETFSRQPTPSIPAACGGWGDTRGAYRFFAHEDIDWQDILKPHWQASAQRMA
EHPVVLCLQDTTELDFNGQGIAGLGRLSYDAQRGMYLHPTYAITPDREPLGVIDAWMWARSAKKGQAASDEVLESTRWIE
GYERIAEQAASLPGTRLVYVADRESDLLALMRRAAELGHPADWLVRAKHNRVLPEDGKLWATVTSGHPLGQIVFTHAPRG
QRARRVHQTVWAKSVRLAQGVTVTCVIAREEHPPSGVKPIEWRLLSNRPAGDLQAATQLIEWYRARWEIELFFHVVKNGC
RIEALQLSRIERLERAIALYLVIAWRLARLMRLGRNGPDLPAELFFEPDEWRGVHFLLKKPIPKQAPRLNTVIRMVATLG
GFLGRKCDGEPGVKTLWIGMQRVADFAAGLDYAREIQAL
>Mature_439_residues
MERMSWAQEEFADLDLGDKRLNRRQVKLAETFSRQPTPSIPAACGGWGDTRGAYRFFAHEDIDWQDILKPHWQASAQRMA
EHPVVLCLQDTTELDFNGQGIAGLGRLSYDAQRGMYLHPTYAITPDREPLGVIDAWMWARSAKKGQAASDEVLESTRWIE
GYERIAEQAASLPGTRLVYVADRESDLLALMRRAAELGHPADWLVRAKHNRVLPEDGKLWATVTSGHPLGQIVFTHAPRG
QRARRVHQTVWAKSVRLAQGVTVTCVIAREEHPPSGVKPIEWRLLSNRPAGDLQAATQLIEWYRARWEIELFFHVVKNGC
RIEALQLSRIERLERAIALYLVIAWRLARLMRLGRNGPDLPAELFFEPDEWRGVHFLLKKPIPKQAPRLNTVIRMVATLG
GFLGRKCDGEPGVKTLWIGMQRVADFAAGLDYAREIQAL

Specific function: Mediates transposition of transposon Tn5 by a 'cut and paste' mechanism. First, the monomeric transposase binds the 19 bp inverted DNA repeats flanking the transposon. Then, dimerization of the DNA-bound transposase creates a synaptic DNA complex. After n

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the transposase 11 family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012337
- InterPro:   IPR002559
- InterPro:   IPR003201
- InterPro:   IPR014737
- InterPro:   IPR014736
- InterPro:   IPR014735 [H]

Pfam domain/function: PF01609 Transposase_11; PF02281 Transposase_Tn5 [H]

EC number: NA

Molecular weight: Translated: 49794; Mature: 49794

Theoretical pI: Translated: 9.13; Mature: 9.13

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MERMSWAQEEFADLDLGDKRLNRRQVKLAETFSRQPTPSIPAACGGWGDTRGAYRFFAHE
CCCCHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCEEEEECC
DIDWQDILKPHWQASAQRMAEHPVVLCLQDTTELDFNGQGIAGLGRLSYDAQRGMYLHPT
CCCHHHHHCHHHHHHHHHHHCCCEEEEEECCCCCCCCCCCCCCCCCCCHHHHCCEEECCE
YAITPDREPLGVIDAWMWARSAKKGQAASDEVLESTRWIEGYERIAEQAASLPGTRLVYV
EEECCCCCCCHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEE
ADRESDLLALMRRAAELGHPADWLVRAKHNRVLPEDGKLWATVTSGHPLGQIVFTHAPRG
ECCCHHHHHHHHHHHHCCCCHHHHHCCCCCCCCCCCCCEEEEEECCCCCEEEEEEECCCC
QRARRVHQTVWAKSVRLAQGVTVTCVIAREEHPPSGVKPIEWRLLSNRPAGDLQAATQLI
HHHHHHHHHHHHHHHHHHCCCEEEEEEEECCCCCCCCCCEEEEEECCCCCCHHHHHHHHH
EWYRARWEIELFFHVVKNGCRIEALQLSRIERLERAIALYLVIAWRLARLMRLGRNGPDL
HHHHHHHHHHHHHHHHHCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
PAELFFEPDEWRGVHFLLKKPIPKQAPRLNTVIRMVATLGGFLGRKCDGEPGVKTLWIGM
CHHHEECCCCCCCCCEEECCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHH
QRVADFAAGLDYAREIQAL
HHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MERMSWAQEEFADLDLGDKRLNRRQVKLAETFSRQPTPSIPAACGGWGDTRGAYRFFAHE
CCCCHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCEEEEECC
DIDWQDILKPHWQASAQRMAEHPVVLCLQDTTELDFNGQGIAGLGRLSYDAQRGMYLHPT
CCCHHHHHCHHHHHHHHHHHCCCEEEEEECCCCCCCCCCCCCCCCCCCHHHHCCEEECCE
YAITPDREPLGVIDAWMWARSAKKGQAASDEVLESTRWIEGYERIAEQAASLPGTRLVYV
EEECCCCCCCHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEE
ADRESDLLALMRRAAELGHPADWLVRAKHNRVLPEDGKLWATVTSGHPLGQIVFTHAPRG
ECCCHHHHHHHHHHHHCCCCHHHHHCCCCCCCCCCCCCEEEEEECCCCCEEEEEEECCCC
QRARRVHQTVWAKSVRLAQGVTVTCVIAREEHPPSGVKPIEWRLLSNRPAGDLQAATQLI
HHHHHHHHHHHHHHHHHHCCCEEEEEEEECCCCCCCCCCEEEEEECCCCCCHHHHHHHHH
EWYRARWEIELFFHVVKNGCRIEALQLSRIERLERAIALYLVIAWRLARLMRLGRNGPDL
HHHHHHHHHHHHHHHHHCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
PAELFFEPDEWRGVHFLLKKPIPKQAPRLNTVIRMVATLGGFLGRKCDGEPGVKTLWIGM
CHHHEECCCCCCCCCEEECCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHH
QRVADFAAGLDYAREIQAL
HHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 6260374; 6271452; 7867940; 6291786; 10884228; 12367522; 11896402 [H]