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The map label for this gene is garL [H]

Identifier: 121610004

GI number: 121610004

Start: 3412685

End: 3413419

Strand: Direct

Name: garL [H]

Synonym: Veis_3060

Alternate gene names: 121610004

Gene position: 3412685-3413419 (Clockwise)

Preceding gene: 121610003

Following gene: 121610011

Centisome position: 61.3

GC content: 69.25

Gene sequence:

>735_bases
ATGAACAGCACGAACCCTGCGCCGATCGGCATCTTCGTCAAGACCGGTTCGCCCCAACTGATCGAGGTGCTGGGCACCAC
GGCACTCGACTTCGTCGTGCTCGATGCCGAGCATGCGCCCTTCGATCGCGGCATGCTCGACCTGATGGTGCTGGGTGGCC
GCGCCGCGCGCCTGCCGGTGTTCATCCGCATTCCCGTGATCGATCCGGCGGTGATTCTCTCCTGCCTGGACATGGGCGCC
ACCGGACTGCTGGTGCCGCATGTCGACACGGCCGAACAGGCGCGCGAAGTGGTCGCCATGGCGCGCTACCGCGGCGGCCG
GCGCGGCTATTCCGGTGGGCCGCGCAGCGCCGGCTATGCGAGCCTGAGCATGGCGCAAGCCGTGCACCTCGGAGACCAGG
CGCAGATCGTCTGCCAGATAGAGAGCGCCCAGGCGGTCGGGCAAGCCCGGGCCATTGCCGAAGTACCGGGCGTGGCGGGC
CTGTTCGTGGGCCGCGCCGATCTGGCGCTGTCGCTGGGACTGGACGATCCGCGCGCCCCCGAAGTGTTGCAGGCGACGCG
CGCGACACTGCAAGCGGCCACACGTGCCGGCAAGCTCGCCGGCATGCATGTGGGCGGCACGCCCGAGCGCGAGCAGTTCA
TCGACGAGGGCGCGAACTGGTTCGTCGTTTCTTCCGACCAGAGTTTGCTGCGGCAGGCCGTGCAGGCCATCGCCGGAGCG
CCGAAGCAGCGCTGA

Upstream 100 bases:

>100_bases
GTCGCCCGAATGGCTGCGCCAGCGCATCCATGCCGACGGCCAGAAATGGCAGCAGGTGATCCGGGCCGCACAGGTCAAGC
TCGACTGAAAGCACGCTCCC

Downstream 100 bases:

>100_bases
AAAAGATCAACCCCGTCGCGCAGTCATCCGTTCAATCTGAATGAGCCAACAAACCCTTGCAATGGCAGCCAATGGCCGCT
GATCGAGGCGCCGGATTCGA

Product: HpcH/HpaI aldolase

Products: NA

Alternate protein names: KDGluc aldolase; KDGlucA; 2-dehydro-3-deoxy-D-glucarate aldolase; 2-keto-3-deoxy-D-glucarate aldolase; 5-dehydro-4-deoxy-D-glucarate aldolase; Alpha-keto-beta-deoxy-D-glucarate aldolase [H]

Number of amino acids: Translated: 244; Mature: 244

Protein sequence:

>244_residues
MNSTNPAPIGIFVKTGSPQLIEVLGTTALDFVVLDAEHAPFDRGMLDLMVLGGRAARLPVFIRIPVIDPAVILSCLDMGA
TGLLVPHVDTAEQAREVVAMARYRGGRRGYSGGPRSAGYASLSMAQAVHLGDQAQIVCQIESAQAVGQARAIAEVPGVAG
LFVGRADLALSLGLDDPRAPEVLQATRATLQAATRAGKLAGMHVGGTPEREQFIDEGANWFVVSSDQSLLRQAVQAIAGA
PKQR

Sequences:

>Translated_244_residues
MNSTNPAPIGIFVKTGSPQLIEVLGTTALDFVVLDAEHAPFDRGMLDLMVLGGRAARLPVFIRIPVIDPAVILSCLDMGA
TGLLVPHVDTAEQAREVVAMARYRGGRRGYSGGPRSAGYASLSMAQAVHLGDQAQIVCQIESAQAVGQARAIAEVPGVAG
LFVGRADLALSLGLDDPRAPEVLQATRATLQAATRAGKLAGMHVGGTPEREQFIDEGANWFVVSSDQSLLRQAVQAIAGA
PKQR
>Mature_244_residues
MNSTNPAPIGIFVKTGSPQLIEVLGTTALDFVVLDAEHAPFDRGMLDLMVLGGRAARLPVFIRIPVIDPAVILSCLDMGA
TGLLVPHVDTAEQAREVVAMARYRGGRRGYSGGPRSAGYASLSMAQAVHLGDQAQIVCQIESAQAVGQARAIAEVPGVAG
LFVGRADLALSLGLDDPRAPEVLQATRATLQAATRAGKLAGMHVGGTPEREQFIDEGANWFVVSSDQSLLRQAVQAIAGA
PKQR

Specific function: Catalyzes the reversible retro-aldol cleavage of both 5- keto-4-deoxy-D-glucarate and 2-keto-3-deoxy-D-glucarate to pyruvate and tartronic semialdehyde [H]

COG id: COG3836

COG function: function code G; 2,4-dihydroxyhept-2-ene-1,7-dioic acid aldolase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the HpcH/HpaI aldolase family. KDGluc aldolase subfamily [H]

Homologues:

Organism=Escherichia coli, GI1789514, Length=241, Percent_Identity=35.6846473029046, Blast_Score=118, Evalue=3e-28,
Organism=Escherichia coli, GI1788578, Length=234, Percent_Identity=32.4786324786325, Blast_Score=98, Evalue=4e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005000
- InterPro:   IPR017648
- InterPro:   IPR015813 [H]

Pfam domain/function: PF03328 HpcH_HpaI [H]

EC number: =4.1.2.20 [H]

Molecular weight: Translated: 25528; Mature: 25528

Theoretical pI: Translated: 6.52; Mature: 6.52

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNSTNPAPIGIFVKTGSPQLIEVLGTTALDFVVLDAEHAPFDRGMLDLMVLGGRAARLPV
CCCCCCCCEEEEEECCCCHHHHHHHHHHEEEEEEECCCCCCCCCCEEEEEECCCCCCCEE
FIRIPVIDPAVILSCLDMGATGLLVPHVDTAEQAREVVAMARYRGGRRGYSGGPRSAGYA
EEEECCCCHHHHHHHHHCCCCEEEECCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCHH
SLSMAQAVHLGDQAQIVCQIESAQAVGQARAIAEVPGVAGLFVGRADLALSLGLDDPRAP
HHHHHHHHHCCCCEEEEEEECCHHHHHHHHHHHHCCCCHHEEECCEEEEEEECCCCCCCH
EVLQATRATLQAATRAGKLAGMHVGGTPEREQFIDEGANWFVVSSDQSLLRQAVQAIAGA
HHHHHHHHHHHHHHHHCCEEEEECCCCCHHHHHHHCCCCEEEEECCHHHHHHHHHHHHCC
PKQR
CCCC
>Mature Secondary Structure
MNSTNPAPIGIFVKTGSPQLIEVLGTTALDFVVLDAEHAPFDRGMLDLMVLGGRAARLPV
CCCCCCCCEEEEEECCCCHHHHHHHHHHEEEEEEECCCCCCCCCCEEEEEECCCCCCCEE
FIRIPVIDPAVILSCLDMGATGLLVPHVDTAEQAREVVAMARYRGGRRGYSGGPRSAGYA
EEEECCCCHHHHHHHHHCCCCEEEECCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCHH
SLSMAQAVHLGDQAQIVCQIESAQAVGQARAIAEVPGVAGLFVGRADLALSLGLDDPRAP
HHHHHHHHHCCCCEEEEEEECCHHHHHHHHHHHHCCCCHHEEECCEEEEEEECCCCCCCH
EVLQATRATLQAATRAGKLAGMHVGGTPEREQFIDEGANWFVVSSDQSLLRQAVQAIAGA
HHHHHHHHHHHHHHHHCCEEEEECCCCCHHHHHHHCCCCEEEEECCHHHHHHHHHHHHCC
PKQR
CCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA