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The map label for this gene is rumA

Identifier: 121610011

GI number: 121610011

Start: 3417939

End: 3419399

Strand: Direct

Name: rumA

Synonym: Veis_3067

Alternate gene names: 121610011

Gene position: 3417939-3419399 (Clockwise)

Preceding gene: 121610004

Following gene: 121610014

Centisome position: 61.4

GC content: 68.86

Gene sequence:

>1461_bases
ATGAGCGAACCGAAAGAACCATCCCCCGCATTGGCGTGCGCCGCACAGGATGGCAGCGGCCTGCCCGAAGGCTGGCTGCA
GGTGCAGTCGATGGACCTCGATGCCCAGGGCGTGGCCCGCAGGGCCGACGGCAAGGTGGTGTTCATCGACGGTGCGCTAC
CGTCGGAATGGGTCAGCGCCAGCACCTACCGAAAGAAAAACCACTGGGAGCAGGCCAACCTGACGGCGATCCACCGCGAA
TCCGCGCAGCGCGTGCGCCCTGGCTGCCCGCATTTTGGCCTGCATGCCGGCGCTTGCGGCGGCTGCAAGATGCAGCATCT
GCATATGGGGGGGCAGGTGGCGGTCAAGCAGCGGGTGCTGGAAGACAACCTCTGGCACCTGGGCCGGGTCAAGCCCGCCA
TGGTGCTGCGCCCCATCGAGGGCCCGGCCTGGGGCTACCGCTACCGGGCCCGCCTGTCCGTGCGCCATGTGATCAAGAAG
GGCGTGGTGCTGGTGGGTTTCCATGAGCGCAAAAGCCGTTACGTGGCCGACATGGCGCAATGCCCCGTGCTGCCGCCCCA
TGCGGCGGCGCTGCTGATGCCGCTGCGCTCGCTGATCGCCGCGCTGGATGCGCATGACAGTTGCCCGCAGATCGAGCTGG
CCTGCGGCGACAGCGTGACCGCGCTGGTGCTGCGCCACCTGCAGCCGCTGAGCGTGGGCGATCACGCCCGGTTGCGCGCC
TTTGCGGCCGAGCACGGCGTGCAATGGTGGCTGCAACCCCGGGGTCCGGACACCGTGCAACCACTGGACGAGGGCGGCGA
GCAACTGTCCTACGCCCTGCCGGACTTCGGCATCACGATGCCGTTCAAGCCCACCGACTTCACCCAGGTCAACCCCCATA
TCAACCGTGTGCTGGTTGCGCGCGCGTTGCGGCTGCTGGCGGTGCGCCCCGATGAGCGCGTGATCGACTGGTTCTGCGGT
CTGGGCAACTTTACGCTGCCACTGGCCACACAGGCGCGTGCGGTGCTGGGCGTCGAAGGCAACGCGGCCCTGGTCGCCCG
TGCGCGCGCGAACCATGGCCTGAACCAGGCCCTGGCGCAGAACCATCGCGTGCTGGCTGCCACCGATTTCGTGGCCTGCA
ACCTGTTCGCGATGACTCCCGGAATGCTGCTGGGCCATGGTGCGGCCGACAAGTGGCTGGTCGACCCGCCCCGCGAGGGC
GCCCTGGCCCTGGTCAAGGCGCTGGCCGACATCGAGCAGTCGCGCCGAGGTGCGCAGGGCGCTACTGCGCTGCCCGCCGG
CGCCGAGGACTGGGTGCCGCCCCGGCGCATTGTCTACGTCAGTTGCAACCCCGCCACGCTGGCGCGGGATGCCGCTTTGC
TGGTGCATCTGGCAGGCTACCAGTGCGCGGCGGCAGGGATGGTGAACATGTTCCCGCACACCGCCCATGTCGAGAGCATC
GCGGTGTTCGAGCGGGGCTGA

Upstream 100 bases:

>100_bases
GATGGCGATCCGGCACTCGAAGATCGCCCGGATTCCGCGGCCGACAAGGCCCCGGGGAACACCGAAAGCCCGGGCCTTGC
GGCCCTGAGACAATCCGGGC

Downstream 100 bases:

>100_bases
AGAAGCTCATCCGCGCCCGGCCGGCATCACGGCATCAGCTGTGCTTGCCGCCGGTGGCGCTATCGTCGCGCGGCCCGGGC
TCGATGTCCGGGGCTTGCTC

Product: 23S rRNA 5-methyluridine methyltransferase

Products: NA

Alternate protein names: 23S rRNA(M-5-U1939)-methyltransferase

Number of amino acids: Translated: 486; Mature: 485

Protein sequence:

>486_residues
MSEPKEPSPALACAAQDGSGLPEGWLQVQSMDLDAQGVARRADGKVVFIDGALPSEWVSASTYRKKNHWEQANLTAIHRE
SAQRVRPGCPHFGLHAGACGGCKMQHLHMGGQVAVKQRVLEDNLWHLGRVKPAMVLRPIEGPAWGYRYRARLSVRHVIKK
GVVLVGFHERKSRYVADMAQCPVLPPHAAALLMPLRSLIAALDAHDSCPQIELACGDSVTALVLRHLQPLSVGDHARLRA
FAAEHGVQWWLQPRGPDTVQPLDEGGEQLSYALPDFGITMPFKPTDFTQVNPHINRVLVARALRLLAVRPDERVIDWFCG
LGNFTLPLATQARAVLGVEGNAALVARARANHGLNQALAQNHRVLAATDFVACNLFAMTPGMLLGHGAADKWLVDPPREG
ALALVKALADIEQSRRGAQGATALPAGAEDWVPPRRIVYVSCNPATLARDAALLVHLAGYQCAAAGMVNMFPHTAHVESI
AVFERG

Sequences:

>Translated_486_residues
MSEPKEPSPALACAAQDGSGLPEGWLQVQSMDLDAQGVARRADGKVVFIDGALPSEWVSASTYRKKNHWEQANLTAIHRE
SAQRVRPGCPHFGLHAGACGGCKMQHLHMGGQVAVKQRVLEDNLWHLGRVKPAMVLRPIEGPAWGYRYRARLSVRHVIKK
GVVLVGFHERKSRYVADMAQCPVLPPHAAALLMPLRSLIAALDAHDSCPQIELACGDSVTALVLRHLQPLSVGDHARLRA
FAAEHGVQWWLQPRGPDTVQPLDEGGEQLSYALPDFGITMPFKPTDFTQVNPHINRVLVARALRLLAVRPDERVIDWFCG
LGNFTLPLATQARAVLGVEGNAALVARARANHGLNQALAQNHRVLAATDFVACNLFAMTPGMLLGHGAADKWLVDPPREG
ALALVKALADIEQSRRGAQGATALPAGAEDWVPPRRIVYVSCNPATLARDAALLVHLAGYQCAAAGMVNMFPHTAHVESI
AVFERG
>Mature_485_residues
SEPKEPSPALACAAQDGSGLPEGWLQVQSMDLDAQGVARRADGKVVFIDGALPSEWVSASTYRKKNHWEQANLTAIHRES
AQRVRPGCPHFGLHAGACGGCKMQHLHMGGQVAVKQRVLEDNLWHLGRVKPAMVLRPIEGPAWGYRYRARLSVRHVIKKG
VVLVGFHERKSRYVADMAQCPVLPPHAAALLMPLRSLIAALDAHDSCPQIELACGDSVTALVLRHLQPLSVGDHARLRAF
AAEHGVQWWLQPRGPDTVQPLDEGGEQLSYALPDFGITMPFKPTDFTQVNPHINRVLVARALRLLAVRPDERVIDWFCGL
GNFTLPLATQARAVLGVEGNAALVARARANHGLNQALAQNHRVLAATDFVACNLFAMTPGMLLGHGAADKWLVDPPREGA
LALVKALADIEQSRRGAQGATALPAGAEDWVPPRRIVYVSCNPATLARDAALLVHLAGYQCAAAGMVNMFPHTAHVESIA
VFERG

Specific function: Catalyzes the formation of 5-methyl-uridine at position 1939 (M-5-U1939) in 23S rRNA

COG id: COG2265

COG function: function code J; SAM-dependent methyltransferases related to tRNA (uracil-5-)-methyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 TRAM domain

Homologues:

Organism=Escherichia coli, GI1789148, Length=463, Percent_Identity=37.5809935205184, Blast_Score=270, Evalue=1e-73,
Organism=Escherichia coli, GI1787083, Length=370, Percent_Identity=25.4054054054054, Blast_Score=76, Evalue=6e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RUMA_VEREI (A1WME3)

Other databases:

- EMBL:   CP000542
- RefSeq:   YP_997818.1
- ProteinModelPortal:   A1WME3
- SMR:   A1WME3
- STRING:   A1WME3
- GeneID:   4692013
- GenomeReviews:   CP000542_GR
- KEGG:   vei:Veis_3067
- NMPDR:   fig|391735.5.peg.2847
- eggNOG:   COG2265
- HOGENOM:   HBG690163
- OMA:   HRADGKV
- PhylomeDB:   A1WME3
- ProtClustDB:   PRK13168
- BioCyc:   VEIS391735:VEIS_3067-MONOMER
- HAMAP:   MF_01010
- InterPro:   IPR016027
- InterPro:   IPR001566
- InterPro:   IPR010280
- TIGRFAMs:   TIGR00479

Pfam domain/function: PF05958 tRNA_U5-meth_tr; SSF50249 Nucleic_acid_OB

EC number: =2.1.1.190

Molecular weight: Translated: 52622; Mature: 52491

Theoretical pI: Translated: 8.50; Mature: 8.50

Prosite motif: PS50926 TRAM; PS01230 TRMA_1; PS01231 TRMA_2

Important sites: ACT_SITE 442-442 BINDING 289-289 BINDING 318-318 BINDING 323-323 BINDING 339-339 BINDING 374-374 BINDING 395-395

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.3 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
2.3 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSEPKEPSPALACAAQDGSGLPEGWLQVQSMDLDAQGVARRADGKVVFIDGALPSEWVSA
CCCCCCCCCCEEEEECCCCCCCHHHEEEEECCCCHHHHHHCCCCCEEEEECCCCHHHHHH
STYRKKNHWEQANLTAIHRESAQRVRPGCPHFGLHAGACGGCKMQHLHMGGQVAVKQRVL
HHHHHHCCCCCCCCHHEEHHHHHHCCCCCCCCCCCCCCCCCCEEEEEECCCHHHHHHHHH
EDNLWHLGRVKPAMVLRPIEGPAWGYRYRARLSVRHVIKKGVVLVGFHERKSRYVADMAQ
HHCCHHHCCCCHHHEEEECCCCCCCEEEEEHHHHHHHHHCCEEEEEEHHHHHHHHHHHHH
CPVLPPHAAALLMPLRSLIAALDAHDSCPQIELACGDSVTALVLRHLQPLSVGDHARLRA
CCCCCHHHHHHHHHHHHHHHHHCCCCCCCCEEEECCCHHHHHHHHHCCCCCCCCHHHHHH
FAAEHGVQWWLQPRGPDTVQPLDEGGEQLSYALPDFGITMPFKPTDFTQVNPHINRVLVA
HHHHCCCEEEECCCCCCCCCCHHCCCCCEEEECCCCCEECCCCCCCCCCCCHHHHHHHHH
RALRLLAVRPDERVIDWFCGLGNFTLPLATQARAVLGVEGNAALVARARANHGLNQALAQ
HHHHHHCCCCCHHHHHHHHCCCCCEECCCCCCEEEEEECCCEEEEEEEHHCCCHHHHHHH
NHRVLAATDFVACNLFAMTPGMLLGHGAADKWLVDPPREGALALVKALADIEQSRRGAQG
CCEEEEEEHHHHEEHHHHCCCCEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCC
ATALPAGAEDWVPPRRIVYVSCNPATLARDAALLVHLAGYQCAAAGMVNMFPHTAHVESI
CCCCCCCCCCCCCCCEEEEEECCCHHHHHHHEEEEEECCCHHHHCCHHHHCCCHHHHHEE
AVFERG
EEEECC
>Mature Secondary Structure 
SEPKEPSPALACAAQDGSGLPEGWLQVQSMDLDAQGVARRADGKVVFIDGALPSEWVSA
CCCCCCCCCEEEEECCCCCCCHHHEEEEECCCCHHHHHHCCCCCEEEEECCCCHHHHHH
STYRKKNHWEQANLTAIHRESAQRVRPGCPHFGLHAGACGGCKMQHLHMGGQVAVKQRVL
HHHHHHCCCCCCCCHHEEHHHHHHCCCCCCCCCCCCCCCCCCEEEEEECCCHHHHHHHHH
EDNLWHLGRVKPAMVLRPIEGPAWGYRYRARLSVRHVIKKGVVLVGFHERKSRYVADMAQ
HHCCHHHCCCCHHHEEEECCCCCCCEEEEEHHHHHHHHHCCEEEEEEHHHHHHHHHHHHH
CPVLPPHAAALLMPLRSLIAALDAHDSCPQIELACGDSVTALVLRHLQPLSVGDHARLRA
CCCCCHHHHHHHHHHHHHHHHHCCCCCCCCEEEECCCHHHHHHHHHCCCCCCCCHHHHHH
FAAEHGVQWWLQPRGPDTVQPLDEGGEQLSYALPDFGITMPFKPTDFTQVNPHINRVLVA
HHHHCCCEEEECCCCCCCCCCHHCCCCCEEEECCCCCEECCCCCCCCCCCCHHHHHHHHH
RALRLLAVRPDERVIDWFCGLGNFTLPLATQARAVLGVEGNAALVARARANHGLNQALAQ
HHHHHHCCCCCHHHHHHHHCCCCCEECCCCCCEEEEEECCCEEEEEEEHHCCCHHHHHHH
NHRVLAATDFVACNLFAMTPGMLLGHGAADKWLVDPPREGALALVKALADIEQSRRGAQG
CCEEEEEEHHHHEEHHHHCCCCEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCC
ATALPAGAEDWVPPRRIVYVSCNPATLARDAALLVHLAGYQCAAAGMVNMFPHTAHVESI
CCCCCCCCCCCCCCCEEEEEECCCHHHHHHHEEEEEECCCHHHHCCHHHHCCCHHHHHEE
AVFERG
EEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA