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The map label for this gene is wcaJ [H]

Identifier: 121609999

GI number: 121609999

Start: 3409211

End: 3409768

Strand: Direct

Name: wcaJ [H]

Synonym: Veis_3055

Alternate gene names: 121609999

Gene position: 3409211-3409768 (Clockwise)

Preceding gene: 121609998

Following gene: 121610000

Centisome position: 61.24

GC content: 67.92

Gene sequence:

>558_bases
GTGAAGCGCCTGCTGGATATCGCGCTGTCTGTGGCGGCGCTGCTCCTGCTCAGCCCGGTCTGGCTCGCCGCCGCGCTGGC
CAGCGCGCTGGGCGACGGTTTTCCGGTGCTGTTCCGGCAGGTGCGGGTGGGGCTGCATGGGCGCGAGTTCCGCATGTACA
AGTTCCGCAGCATGGTGCGCCAGGCGGACCGCCTCGGCCCCCATTTCACGACGGGCGGTGACGACCCGCGCATCACGCCG
GTGGGGCGCTGGCTGCGGCGCACCAGCATCGACGAATTGCCGCAACTGCTGAATGTGCTCAAGGGCGACATGAGCCTGGT
CGGCCCCCGCCCCGACCTGCCGGTGCAGCGTGATCTCTACACCGAGGCGCAATGGCTGGCACGTTGCAGCGTTCGGCCCG
GCATCACCGGTTTGGCGCAGGCGCTGTACCGTTCAGAGGCCACGCATGAGCAGCGACTGGCGGCAGATTTGCGCTACGCA
CGCGAAATGAGCCTGTGGCTGGATCTGCAAATCATGCTGTGGAGCCTGCGGCGGTTGGGCGGCAAGGCGGCGAATTAG

Upstream 100 bases:

>100_bases
AGCGCGATCTGGCCGCACGCAGCGCCGATCAGGTCGAGCGCGACCGCTGGCGCATCCGTTCGGCAATAGCCGGCCTTGCC
GCAGGCATGGGGCCTGCCGC

Downstream 100 bases:

>100_bases
CCTGGCCACCGGAATTGCCGGGGCCTGCTAGTGTCGCGTCACGGATCAGATGTCGTAGGCTGCGCGCAGCCATCGGAGCG
TAGCGCAAGGCGCATCGCGC

Product: sugar transferase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 185; Mature: 185

Protein sequence:

>185_residues
MKRLLDIALSVAALLLLSPVWLAAALASALGDGFPVLFRQVRVGLHGREFRMYKFRSMVRQADRLGPHFTTGGDDPRITP
VGRWLRRTSIDELPQLLNVLKGDMSLVGPRPDLPVQRDLYTEAQWLARCSVRPGITGLAQALYRSEATHEQRLAADLRYA
REMSLWLDLQIMLWSLRRLGGKAAN

Sequences:

>Translated_185_residues
MKRLLDIALSVAALLLLSPVWLAAALASALGDGFPVLFRQVRVGLHGREFRMYKFRSMVRQADRLGPHFTTGGDDPRITP
VGRWLRRTSIDELPQLLNVLKGDMSLVGPRPDLPVQRDLYTEAQWLARCSVRPGITGLAQALYRSEATHEQRLAADLRYA
REMSLWLDLQIMLWSLRRLGGKAAN
>Mature_185_residues
MKRLLDIALSVAALLLLSPVWLAAALASALGDGFPVLFRQVRVGLHGREFRMYKFRSMVRQADRLGPHFTTGGDDPRITP
VGRWLRRTSIDELPQLLNVLKGDMSLVGPRPDLPVQRDLYTEAQWLARCSVRPGITGLAQALYRSEATHEQRLAADLRYA
REMSLWLDLQIMLWSLRRLGGKAAN

Specific function: Slime polysaccharide colanic acid biosynthesis. [C]

COG id: COG2148

COG function: function code M; Sugar transferases involved in lipopolysaccharide synthesis

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial sugar transferase family [H]

Homologues:

Organism=Escherichia coli, GI1788360, Length=186, Percent_Identity=43.5483870967742, Blast_Score=141, Evalue=3e-35,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003362
- InterPro:   IPR017475
- InterPro:   IPR017473 [H]

Pfam domain/function: PF02397 Bac_transf [H]

EC number: NA

Molecular weight: Translated: 20859; Mature: 20859

Theoretical pI: Translated: 11.07; Mature: 11.07

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKRLLDIALSVAALLLLSPVWLAAALASALGDGFPVLFRQVRVGLHGREFRMYKFRSMVR
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCHHHHHHHHHHHH
QADRLGPHFTTGGDDPRITPVGRWLRRTSIDELPQLLNVLKGDMSLVGPRPDLPVQRDLY
HHHHCCCCCCCCCCCCCCCHHHHHHHHCCHHHHHHHHHHHHCCHHHCCCCCCCCCHHHHH
TEAQWLARCSVRPGITGLAQALYRSEATHEQRLAADLRYAREMSLWLDLQIMLWSLRRLG
HHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
GKAAN
CCCCC
>Mature Secondary Structure
MKRLLDIALSVAALLLLSPVWLAAALASALGDGFPVLFRQVRVGLHGREFRMYKFRSMVR
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCHHHHHHHHHHHH
QADRLGPHFTTGGDDPRITPVGRWLRRTSIDELPQLLNVLKGDMSLVGPRPDLPVQRDLY
HHHHCCCCCCCCCCCCCCCHHHHHHHHCCHHHHHHHHHHHHCCHHHCCCCCCCCCHHHHH
TEAQWLARCSVRPGITGLAQALYRSEATHEQRLAADLRYAREMSLWLDLQIMLWSLRRLG
HHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
GKAAN
CCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 8759852; 9097040; 9278503 [H]